Applicability Assessment of Different Materials for Standards Ensuring Comparability of Optical and Tactile Coordinate Measurements

Multisensor CMMs are systems with an established position on the market, but their popularity still grows, as they provide access to the advantages offered by tactile and contactless measurement methods. Yet there are still questions of the comparability of results obtained using the optical and tactile operation modes of multisensor system. This phenomenon can be assessed by measuring appropriate gauges, most often reference rings or spheres. Due to the completely different nature of probing processes for tactile and contactless measurements, the material from which reference object is made may significantly affect measurement results. In order to assess the influence of this factor on measurement accuracy, three reference spheres made from different materials were measured on optical multisensor CMMs. Measurements involved tactile measurements as well as optical measurements made using different probing systems: a video probe and white light sensor. Results obtained from performed experiments show large differences depending on the material used for spherical standard production. On the basis of obtained results, it can be stated that the best material for a reference object that can be used for comparability tests of tactile and optical measurements is a composite of alumina with at least one oxidic additive.     

GL Beams Reinforced with Plywood in the Outer Layer

Glulam beams are increasingly used in the construction industry because of their high strength and the possibility of using round timber with smaller cross-sections. The load-bearing capacity of beams is strongly related to the quality of the outer layers and, in the case of wood, especially the tension zones. For these reasons, this study decided to replace the outer lamella with tensile plywood. The produced beams were subjected to static bending strength and modulus of elasticity evaluation. It was shown that the best static bending strength values were obtained for beams containing plywood in the tension layer. However, the change in structure in the tension zone of beams made of glued laminated timber results not only in an increase in the load capacity of elements produced in this way but also in a decrease in the range/range of the obtained results of bending strength. This way of modifying the construction of glued laminated beams allows a more rational use of available pine timber.     

Growth and development of trabecular structure in the calcaneus of Japanese macaques (Macaca fuscata) reflects locomotor behavior,life history,and neuromuscular development

Bone structure dynamically adapts to its mechanical environment throughout ontogeny by altering the structure of trabecular bone, the three‐dimensional mesh‐like structure found underneath joint surfaces. Trabecular structure, then, can provide a record of variation in loading directions and magnitude; and in ontogenetic samples, it can potentially be used to track developmental shifts in limb posture. We aim to broaden the analysis of trabecular bone ontogeny by incorporating interactions between ontogenetic variation in locomotor repertoire, neuromuscular maturation, and life history. We examine the associations between these variables and age‐related variation in trabecular structure in the calcaneus of Japanese macaques (Macaca fuscata). We used high‐resolution micro‐computed tomography scanning to image the calcaneus in a cross‐sectional sample of 34 juvenile M. fuscata aged between 0 and 7 years old at the Primate Research Institute, Japan. We calculated whole bone averages of standard trabecular properties and generated whole‐bone morphometric maps of bone volume fraction and Young’s modulus. Trabecular structure becomes increasingly heterogeneous in older individuals. Bone volume fraction (BV/total volume [TV]) decreases during the first month of life and increases afterward, coinciding with the onset of independent locomotion in M. fuscata. At birth, primary Young’s modulus is oriented orthogonal to the ossification center, but after locomotor onset bone structure becomes stiffest in the direction of joint surfaces and muscle attachments. Age‐related variation in bone volume fraction is best predicted by an interaction between the estimated percentage of adult brain size, body mass, and locomotor onset. To explain our findings, we propose a model where interactions between age‐related increases in body weight and maturation of the neuromuscular system alter the loading environment of the calcaneus, to which the internal trabecular structure dynamically adapts. This model cannot be directly tested based on our cross‐sectional data. However, confirmation of the model by longitudinal experiments and in multiple species would show that trabecular structure can be used both to infer behavior from fossil morphology and serve as a valuable proxy for neuromuscular maturation and life history events like locomotor onset and the achievement of an adult‐like gait. This approach could significantly expand our knowledge of the biology and behavior of fossil species.     

INAUGURAL ARTICLE by a Recently Elected Academy Member:Sustained stoichiometric imbalance and its ecological consequences in a large oligotrophic lake

Considerable attention is given to absolute nutrient levels in lakes, rivers, and oceans, but less is paid to their relative concentrations, their nitrogen:phosphorus (N:P) stoichiometry, and the consequences of imbalanced stoichiometry. Here, we report 38 y of nutrient dynamics in Flathead Lake, a large oligotrophic lake in Montana, and its inflows. While nutrient levels were low, the lake had sustained high total N: total P ratios (TN:TP: 60 to 90:1 molar) throughout the observation period. N and P loading to the lake as well as loading N:P ratios varied considerably among years but showed no systematic long-term trend. Surprisingly, TN:TP ratios in river inflows were consistently lower than in the lake, suggesting that forms of P in riverine loading are removed preferentially to N. In-lake processes, such as differential sedimentation of P relative to N or accumulation of fixed N in excess of denitrification, likely also operate to maintain the lake’s high TN:TP ratios. Regardless of causes, the lake’s stoichiometric imbalance is manifested in P limitation of phytoplankton growth during early and midsummer, resulting in high C:P and N:P ratios in suspended particulate matter that propagate P limitation to zooplankton. Finally, the lake’s imbalanced N:P stoichiometry appears to raise the potential for aerobic methane production via metabolism of phosphonate compounds by P-limited microbes. These data highlight the importance of not only absolute N and P levels in aquatic ecosystems, but also their stoichiometric balance, and they call attention to potential management implications of high N:P ratios.

The emergence of the Anthropocene era has been marked by major changes in all of Earth’s major biogeochemical cycles (1). For example, fluxes of carbon (C) (as CO₂) to the atmosphere have increased by ∼14% during the last 120 y largely due to fossil fuel combustion. Fluxes of nitrogen (N) into the biosphere have increased by at least 100% due to application of the Haber-Bosch reaction for fertilizer production, land use change favoring N-fixing legumes, and conversion of atmospheric N₂ to available forms (NO_x) by high temperature combustion of petroleum and fossil gas (2). Finally, large-scale mining of phosphorus (P)-rich geological deposits for production of fertilizers has amplified rates of P cycling in the biosphere by ∼400% (1). Each of these perturbations has biophysical and ecological impacts at differing time and space scales. For C, its accumulation in the atmosphere has altered Earth’s radiative balance, warming the planet and perturbing precipitation patterns globally. Amplified inputs of reactive N to the Earth system enter the hydrosphere and, thus, potentially lead to overenrichment of lakes, rivers, and coastal oceans across broad regions. Amplifications of P inputs often impair water quality at watershed and local scales (3), stimulating phytoplankton production and contributing, along with N, to harmful algal blooms, fish kills, and “dead zones” (4, 5). These differential amplifications and their contrasting spatial scales indicate that ecosystems are experiencing not only absolute changes in biogeochemical cycling, but also perturbations in the relative inputs and outputs of biologically important elements (6). Studies of elemental coupling and uncoupling in ecosystems are not yet widespread, but emerging work has shown how C, N, and P are differentially processed as they pass through watersheds (7).The potential for differential alteration in supplies of N and P to aquatic ecosystems suggests that understanding the nutrient status of a water body requires knowledge of not only absolute supplies of limiting nutrients, but also their relative proportions (i.e., their N:P stoichiometry). This work has been facilitated in recent years by the emergence of the theory of ecological stoichiometry (8). For example, seminal work by Redfield (9) found that N:P ratios in marine organic matter were tightly constrained around 16:1 (molar, here and throughout), a value that may represent the central tendency for the N:P ratio of phytoplankton undergoing balanced growth in which major pools of N (protein) and P (RNA) are produced at the same rate (10). In lakes, N:P ratios show much wider variation—around a value of ∼30—perhaps reflecting the biogeochemical connections of lakes to terrestrial systems where N:P ratios have a similar value and range of variation (11). Nevertheless, this classic “Redfield ratio” of 16:1 can be thought of as representing a balanced nutrient supply for primary producers in pelagic ecosystems. When the ratios of N and P supplied deviate from this balanced ratio, primary limitation of growth by N (when N:P is low) or by P (when N:P is high) can occur. For example, phytoplankton growth in lakes with imbalanced total N: total P (TN:TP) ratios that exceed 30:1 is generally P limited (12). Disproportionate inputs of N relative to P from atmospheric deposition can increase lake TN:TP ratios and shift lake phytoplankton from N to P limitation (13), inducing P limitation in zooplankton (14). Imbalanced N:P ratios in nutrient supplies can also shift the competitive advantage among phytoplankton and enhance production of potentially toxic compounds during harmful algal blooms. For example, skewed supplies of N relative to P can increase production of N-rich secondary compounds by phytoplankton, while disproportionate inputs of P relative to N can induce production of C-based toxins (15). High N:P ratios can also enhance proliferation of fungal parasites of phytoplankton (16).Imbalanced N:P ratios can impact aquatic ecosystems in other ways. For example, they can alter the functioning of food webs. In particular, shifts in nutrient supply regimes that enhance P limitation can impede energy flow in trophic interactions because biomass of P-limited primary producers is of low quality for animals due to its low P content (8). Ecosystem shifts to high N:P ratios and more prevalent P limitation can also impact the cycling of the greenhouse-active gas methane (CH₄) because phosphate limitation can result in production of methane under aerobic conditions in both marine and freshwater phytoplankton and bacteria (17, 18). Both chemoheterotrophic and photoautotrophic bacteria (e.g., Pseudomonas, SAR11, Trichodesmium, Synechococcus) can metabolize organic P compounds, called phosphonates, to acquire P. Microbial cleavage of one type of phosphonate, methylphosphonic acid (MPn), to acquire P results in formation of methane (17). While it is likely that anaerobic methane production due to oxygen depletion in response to P-driven eutrophication is the dominant process connecting P to methane dynamics, the significance of aerobic phosphonate metabolism to global methane cycles remains to be assessed. However, contributions are potentially large, given the prevalence of P limitation in both freshwater and marine ecosystems. In light of emerging trends that suggest overall increases in ecosystem N:P ratios due to human impacts (6), these trophic and biogeochemical impacts of stoichiometric imbalance show that it is critical to consider not only absolute levels of nutrients, but also their stoichiometry. In particular, high N:P ratios can accentuate P limitation, causing a suite of ecological impacts that, currently, are poorly described.In this paper, we illustrate the utility of stoichiometric approaches by combining analyses of long-term records of nutrient supply and dynamics, together with contemporary experiments, to examine how imbalances in N:P stoichiometry (e.g., strong divergence from classic Redfield proportions) influence plankton ecology and biogeochemistry across multiple scales in Flathead Lake, a large lake in western Montana. The lake is itself relatively unperturbed by human impacts and, thus, maintains low overall nutrient levels. However, the strong stoichiometric imbalance that we describe makes Flathead Lake appropriate for assessing ecosystem consequences of what appear to be general trends of increasing N:P ratios in global ecosystems (6). Numerous limnological properties of the lake and its inflow rivers have been monitored continuously for several decades, including concentrations of various forms of N and P. Thus, these time-series data allow us not only to assess long-term variability or stability in the stoichiometry of N and P in the lake and its river inflows over decadal time scales, but also to connect its stoichiometry with potential consequences for nutrient limitation, food web dynamics, and biogeochemical cycling under low-nutrient conditions.     

Current Evidence of Watermelon (Citrullus lanatus) Ingestion on Vascular Health: A Food Science and Technology Perspective

The amino acid L-arginine is crucial for nitric oxide (NO) synthesis, an important molecule regulating vascular tone. Considering that vascular dysfunction precedes cardiovascular disease, supplementation with precursors of NO synthesis (e.g., L-arginine) is warranted. However, supplementation of L-citrulline is recommended instead of L-arginine since most L-arginine is catabolized during its course to the endothelium. Given that L-citrulline, found mainly in watermelon, can be converted to L-arginine, watermelon supplementation seems to be effective in increasing plasma L-arginine and improving vascular function. Nonetheless, there are divergent findings when investigating the effect of watermelon supplementation on vascular function, which may be explained by the L-citrulline dose in watermelon products. In some instances, offering a sufficient amount of L-citrulline can be impaired by the greater volume (>700 mL) of watermelon needed to reach a proper dose of L-citrulline. Thus, food technology can be applied to reduce the watermelon volume and make supplementation more convenient. Therefore, this narrative review aims to discuss the current evidence showing the effects of watermelon ingestion on vascular health parameters, exploring the critical relevance of food technology for acceptable L-citrulline content in these products. Watermelon-derived L-citrulline appears as a supplementation that can improve vascular function, including arterial stiffness and blood pressure. Applying food technologies to concentrate bioactive compounds in a reduced volume is warranted so that its ingestion can be more convenient, improving the adherence of those who want to ingest watermelon products daily.     

Insights into Genetic Characteristics and Virological Features of Endemic Avian Influenza A (H9N2) Viruses in Egypt from 2017–2021

From 2010 to 2013, genotype I avian influenza A(H9N2) viruses of the G1-lineage were isolated from several poultry species in Egypt. In 2014, novel reassortant H9N2 viruses were detected in pigeons designated as genotype II. To monitor the subsequent genetic evolution of Egyptian A(H9N2) viruses, we characterized the full genomes of 173 viruses isolated through active surveillance from 2017 to 2022. In addition, we compared the virological characteristics and pathogenicity of representative viruses. Phylogenetic analysis of the HA indicated that all studied sequences from 2017–2021 were grouped into G1-like H9N2 viruses previously detected in Egypt. Phylogenetic analysis indicated that the Egyptian A(H9N2) viruses had undergone further reassortment, inheriting four genes (PB2, PB1, PA, NS) from genotype II, with their remaining segments deriving from genotype I viruses (these viruses designated as genotype III). Studying the virological features of the two most dominant genotypes (I and III) of Egyptian H9N2 viruses in vitro and in vivo indicated that both replicated well in mammalian cells, but did not show any clinical signs in chickens, ducks, and mice. Monitoring avian influenza viruses through surveillance programs and understanding the genetic and antigenic characteristics of circulating H9N2 viruses are essential for risk assessment and influenza pandemic preparedness.     

Kinetics of the SARS-CoV-2 Antibody Avidity Response Following Infection and Vaccination

Serological assays capable of measuring antibody responses induced by previous infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been critical tools in the response to the COVID-19 pandemic. In this study, we use bead-based multiplex assays to measure IgG and IgA antibodies and IgG avidity to five SARS-CoV-2 antigens (Spike (S), receptor-binding domain (RBD), Nucleocapsid (N), S subunit 2, and Membrane-Envelope fusion (ME)). These assays were performed in several cohorts of healthcare workers and nursing home residents, who were followed for up to eleven months after SARS-CoV-2 infection or up to six months after vaccination. Our results show distinct kinetic patterns of antibody quantity (IgG and IgA) and avidity. While IgG and IgA antibody levels waned over time, with IgA antibody levels waning more rapidly, avidity increased with time after infection or vaccination. These contrasting kinetic patterns allow for the estimation of time since previous SARS-CoV-2 infection. Including avidity measurements in addition to antibody levels in a classification algorithm for estimating time since infection led to a substantial improvement in accuracy, from 62% to 78%. The inclusion of antibody avidity in panels of serological assays can yield valuable information for improving serosurveillance during SARS-CoV-2 epidemics.     

Assessment of the Impact of Ionizing Radiation Absorption on the Structural,Mechanical and Biophysical Properties of Textiles Used in Multilayer Space Suit

The article presents research on ergonomics, biophysical comfort and safety of protective clothing. The resistance of the structural, thermal and mechanical properties of five fabrics (CBXS400, GG200T, Twaron CT736, Dyneema HB26 and T1790C), differing in geometry and raw material composition used in space suits, to dangerous ionizing radiation (β and γ) occurring in space was tested. For both types of radiation, four identical one-time doses in the range of 25–100 kGy were used. The effect of the applied absorbed doses of β and γ radiation on the parameters of textiles influencing ergonomics and safety of the cosmonaut’s work was verified by structural tests (micro-computed tomography and optical microcopy), thermal resistance tests (sweating guarded-hotplate) and strength tests (tensile testing machine). Experimental studies of thermal properties are supplemented with heat transport simulations using the finite volume method performed with 3D models of real textiles. The greatest reduction of thermal resistance for Twaron CT736 (−0.0667 m²·°C·W⁻¹ for 100 kGy of β-radiation) and Dyneema HB26 (−0.0347 m²·°C·W⁻¹ for 50 kGy of β-radiation) is observed. Strength tests have shown that all tested textiles are resistant to both types of radiation. Three textiles were selected to create a three-layer assembly with potential application in a cosmonaut’s glove (Extravehicular Activity—EVA).     

Modelling of the Flow in the Process of Washing Out Automotive Catalyst Carriers with the PbLi Alloy

The process of platinum recovery from used car catalysts is highly desirable for both economic and environmental reasons. From the many available methods of processing used car catalysts, the article conducted both numerical and experimental studies using a device based on the collector metal method with lead as a modified medium through a magnetohydrodynamic pump for washing platinum from the channels of the ceramic catalyst carrier. It was assumed that lead alloys with the addition of lithium increase the extraction of platinum from thin catalytic layers and accelerate the platinum dissolution reaction in the Pb-Li alloy, which is the result of a greater affinity of lithium for platinum compared to lead. This assumption was verified by numerical simulations as well as laboratory tests. Tests were carried out for the secondary supply voltage range between 40 and 60 V and the catalyst flushing time between 240 and 480 s. Graphical results of the research were discussed.     

Neoadjuvant therapy and biliary decompression lower biliary and pancreatic juice vascular endothelial growth factor levels in patients with periampullary adenocarcinoma

Increased expression of vascular endothelial growth factor (VEGF) by pancreatic cancer correlates with poor survival. The significance of VEGF in biliary and pancreatic secretions in periampullary cancers is unknown. Bile and pancreatic juice samples were collected from patients undergoing pancreaticoduodenectomy (PD). All samples were frozen at -70 degrees C until subsequent analysis for VEGF concentration using enzyme-linked immunoabsorbent assay (ELISA). Plasma VEGF levels in pancreatic cancer patients were <10 pg/mL. The biliary VEGF concentration for patients with malignancy was significantly elevated compared to benign disease (P = 0.05). There was no difference in pancreatic VEGF concentrations between benign and malignant disease. Cancer patients undergoing preoperative chemoradiation (CRT) had lower biliary and pancreatic VEGF concentrations than those who did not. Preoperative biliary drainage (BD) was associated with decreased VEGF concentrations in bile (3500 pg/mL vs 7740 pg/mL, P = 0.027). Patients undergoing both CRT and BD had diminished biliary and pancreatic VEGF concentrations compared to those who had neither. This was statistically significant for pancreatic VEGF concentrations (917 pg/mL vs 4723 pg/mL, P = 0.044). VEGF is highly concentrated in bile and pancreatic juice compared to plasma. Preoperative CRT and BD significantly reduce these levels in patients with periampullary cancers. Antiangiogenic therapy aimed at interrupting the VEGF pathway appears to be a logical target in periampullary cancer.