k阿片受体激动剂抑制大鼠吗啡戒断症状的发生

用多次注射吗啡的方法造成大鼠对吗啡的依赖，给大鼠脊髓蛛网下腔（ｉ．ｔ．）注射ｋ（ｋａｐｐａ）阿片受体激动剂Ｕ－５０，４８８Ｈ（２．５、５、１０μｌ）或ｋ受体拮抗剂ｎｏｒ—ＢＮＩ（１．２５、２．５、５μｌ／１０μｌ）后，用纳洛酮（ＮＸ）催瘾，观察并记录四种戒断症状：湿科（ｗｅｔｓｈａｋｅｓ）、咬牙（ｔｅｅｔｈｃｈａｔｔｅｒｉｎｇ）、逃跑企图（ｅｓｃａｐｅａｔｔｅｍｐｔｓ）、体重丢失（ｗｅｉｇｈｔｌｏｓｅ）。结果表明Ｕ—５０，４８８Ｈ可以明显减轻湿抖、咬牙和逃跑企图，并呈量效关系；ｎｏｒ—ＢＮＩ则使温抖、咬牙和体重丢失三种症状明显加重，也呈量效关系。上述结果表明，激活脊髓。受体对吗啡戒断症状有明显的抑制作用，阻断Ｋ受体则加重戒断症状。     

透皮促进剂对胰岛素离子导入大鼠体内血糖水平的影响

研究了几种常用透皮促进剂如月桂氮　酮(azone,AZ),油酸(oleicacid,OA),泊洛沙姆(poloxamer188,POL188)和丙二醇(propyleneglycol,PG)等对胰岛素离子导入大鼠体内血糖水平的影响。结果显示:离子导入3h后,100%AZ和5%AZ/PG处理组,处理后对处理前的大鼠血糖变化百分率(D%)分别为38.58%和25.28%,而对照组的为45.48%。100%OA,10%OA/PG,10%POL188%和100%PG处理组的D%分别为66.28%,60.47%,76.75%和57.93%。表明月桂氮　酮与离子导入有协同作用,丙二醇能够增强这种作用,3者并用有特别显著的促渗作用。而油酸、泊洛沙姆、丙二醇不增强离子导入的作用。离子导入和某些透皮促进剂并用,为多肽类药物的体内给药提供了新的思路和可能性。     

Noninterference of isosulfan blue on estrogen-receptor activity

Because a 1% sterile solution of methylene blue used for occult breast tumor localization has been shown to interfere with the estrogen-receptor protein (ERP) binding-capacity assay, isosulfan blue in a 1% injection was studied as a potential alternate stain. Cytosols derived from ERP-positive lyophilized powders and human breast tissue were evaluated with and without varying levels of treatment with isosulfan blue. No modification of the ERP-specific binding capacity was found with this stain. The use of isosulfan blue for localization of occult breast tumor is suggested when an ERP binding capacity assay is anticipated.