全文获取类型
收费全文 | 478篇 |
免费 | 15篇 |
专业分类
儿科学 | 62篇 |
妇产科学 | 6篇 |
基础医学 | 34篇 |
口腔科学 | 18篇 |
临床医学 | 46篇 |
内科学 | 112篇 |
皮肤病学 | 16篇 |
神经病学 | 32篇 |
外科学 | 73篇 |
综合类 | 4篇 |
预防医学 | 12篇 |
药学 | 47篇 |
肿瘤学 | 31篇 |
出版年
2022年 | 4篇 |
2017年 | 4篇 |
2016年 | 7篇 |
2015年 | 8篇 |
2014年 | 6篇 |
2013年 | 5篇 |
2012年 | 6篇 |
2011年 | 4篇 |
2010年 | 10篇 |
2009年 | 8篇 |
2008年 | 7篇 |
2007年 | 9篇 |
2006年 | 23篇 |
2005年 | 10篇 |
2004年 | 19篇 |
2003年 | 14篇 |
2002年 | 8篇 |
2001年 | 3篇 |
2000年 | 4篇 |
1999年 | 8篇 |
1998年 | 34篇 |
1997年 | 46篇 |
1996年 | 35篇 |
1995年 | 26篇 |
1994年 | 26篇 |
1993年 | 24篇 |
1992年 | 10篇 |
1991年 | 6篇 |
1990年 | 8篇 |
1989年 | 7篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 10篇 |
1985年 | 2篇 |
1984年 | 6篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1981年 | 8篇 |
1980年 | 9篇 |
1979年 | 6篇 |
1978年 | 5篇 |
1977年 | 5篇 |
1975年 | 3篇 |
1973年 | 3篇 |
1972年 | 3篇 |
1971年 | 2篇 |
1959年 | 2篇 |
1958年 | 3篇 |
1956年 | 3篇 |
1955年 | 6篇 |
排序方式: 共有493条查询结果,搜索用时 15 毫秒
11.
Human T cell responses to recombinant mite antigens of Dermatophagoides farinae 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Clinical and experimental immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
We studied T cell responses to four glutathione S transferase (GST)-fused mite antigens prepared in our laboratory using peripheral blood lymphocytes from mite-sensitive patients with bronchial asthma. Of the four recombinant antigens, purified GST-Mag3 had the strongest ability to cause patients' lymphocytes to proliferate, and its potency was almost comparable to that of crude mite bodies (Dfb) and faeces (Dff) antigens and a purified major antigen, Der f 2. The responder lymphocytes were mainly T cells, because the proliferative response was depleted by the treatment of lymphocytes with anti-CD3 antibody and complement, but not with anti-CD20 antibody and complement. The responsiveness of lymphocytes to GST-Mag3 correlated with that to Der f 2, but GST-Mag3 displayed slightly higher activity to stimulate lymphocytes than Der f 2. Simultaneously, the levels of Dff- and GST-Mag3-specific IgE antibodies correlated with the responsiveness of lymphocytes to GST-Mag3. These results suggest that Mag3 is a new valuable antigen for the response of T cell proliferation in mite-sensitive patients. 相似文献
12.
ALCOHOL-RELATED SUDDEN DEATH WITH HEPATIC FATTY METAMORPHOSIS: A COMPREHENSIVE CLINICOPATHOLOGICAL INQUIRY INTO ITS PATHOGENESIS 总被引:1,自引:0,他引:1
YUZURIHA TAKEFUMI; OKUDAIRA MASAHIKO; TOMINAGA ITARU; HORI SHINGO; SUZUKI HIROMICHI; MATSUO YOSHIHIRO; SHOJI MUNESUKE; YOKOYAMA AKIRA; TAKAGI SATOSHI; HAYASHIDA MOTOI 《Alcohol and alcoholism (Oxford, Oxfordshire)》1997,32(6):745-752
To clarify the pathogenesis of the widely known but obscuresyndrome of sudden death with hepatic fatty metamorphosis observedin alcohol abusers, we have scrutinized both the clinical andpathological data of 11 subjects who died under such circumstancesbetween 1987 and 1993. Death followed several days of uninterrupteddrinking often with little dietary intake. The notable clinicalfeatures on arrival at the emergency room were disturbance ofconsciousness (11/11), hypotension (47/6), hypothermia (3/5),hypoglycaemia (8/11), metabolic acidosis (6/6), renal dysfunction(11/11), and hyperammonaemia (5/5). The common hepatic pathologywas the extensive appearance of numerous microvesicular fattydroplets in the hepatocytes together with varying degrees ofmacrovesicular fatty change; four subjects had an underlyingcirrhosis. Death undoubtedly results from a variety of metabolicdisturbances triggered by the combination of massive ethanolintake and starvation. The appearance of extensive microvesicularfatty change superimposed on macrovesicular fatty change wasconsidered to be an associated phenomenon 相似文献
13.
MASAOMI CHINUSHI YOSHIFUSA AIZAWA TOSHIKAZU FUNAZAKI MAKOTO TAMURA AKIRA SHIBATA 《Pacing and clinical electrophysiology : PACE》1992,15(5):756-761
In a patient with sustained ventricular tachycardia, we obtained two different paced QRS morphologies from a single pacing site. In one QRS morphology the stimulus to the QRS complex was long, 150 msec, and in the other it was 100 msec. At the paced cycle length of 600 msec and the stimulus output of 4 V, one QRS morphology with the stimulus to the onset of QRS activation (St-QRS) interval of 150 msec was observed. At the paced cycle length of 400 msec, the other QRS morphology with a St-QRS interval of 100 msec was observed alternatively with the former. At the paced cycle length of 353 msec or 316 msec, the latter with a shorter St-QRS interval was exclusively observed. When the stimulus output was increased from 4 to 10 V, keeping with the paced cycle length at 400 msec, the St-QRS interval was shortened from 100 to 80 msec. For the two QRS morphologies with two St-QRS intervals, two slowly conducting pathways would be responsible. The site of the block in the faster pathway must be located at the proximity of the pacing site and the conduction at a shorter paced cycle length would be explained by "supernormal conduction." 相似文献
14.
Y. FUJITA N. FUJITA T. NAKAMURA T. GOTO T. FUJII N. HIRAMORI T. KIDA M. SAKAI Y. NAKAGAKI Y. IWASAKI M. NAKAGAWA 《Scandinavian journal of immunology》1992,36(4):597-606
To clarify the significance of basic fetoprotein (BFP) in lymphocytes, we investigated whether BFP is produced in lymphocytes during blastic transformation. Peripheral blood lymphocytes obtained from 14 adults were cultured under the stimulation of lectins. The concentration of BFP in the culture medium (extracellular BFP) was estimated serially. The incorporation of [6-3 H] thymidine was assayed simultaneously. The intracellular BFP was measured by dual flow cytometry for DNA and BFP. A lymph node was studies immunohistochemically. Serum BFP was measured in four cases of lumphocytic leukaemia. In two cases, dual staining was performed. The intracellular BFP of the mitogen-stimulated lymphocytes was increased within 24 h. The extracellular BFP was increased exponentially from 72 h. The extracellular BFP at 96 h did not correlate with the [3 H]-thymidine incorporation. The intracellular BFP increase began in G1 phase. Immunostaining showed that the B cells also produced BFP. The
serum BFP level in leukaemia was high in 1 of 4 cases and the leukaemic cells in two cases showed high intracellular BFP content. These observations indicate that BFP is produced in activated human lymphocytes and in lymphocytic leukaemic cells. The production of BFP during blastic transformation will be a useful new in vitro model for studying the biological role of BFP, and BFP labelling may offer some new possibilities for study of lymphocytes. 相似文献
serum BFP level in leukaemia was high in 1 of 4 cases and the leukaemic cells in two cases showed high intracellular BFP content. These observations indicate that BFP is produced in activated human lymphocytes and in lymphocytic leukaemic cells. The production of BFP during blastic transformation will be a useful new in vitro model for studying the biological role of BFP, and BFP labelling may offer some new possibilities for study of lymphocytes. 相似文献
15.
TAKADA AKIRA; TSUTSUMI MIKIHIRO; KOBAYASHI YUKI 《Alcohol and alcoholism (Oxford, Oxfordshire)》1994,29(6):719-727
Genetic factors related to the development of alcoholic liverand pancreatic diseases (ALD and APD) and of alcohol-inducedasthma were analyzed. The development of ALD is geneticallycontrolled and is directly associated with the polymorphismsof the genes of acetaldehyde (Ac-CHO) and ethanol-metabolizingenzymes, aldehyde dehydrogenase-2 (ALDH2) and cytochrome P4502E1.The development of ALD and APD may also be genetically linkedwith the induction of gamma-glutamyl transferase (GTT) by alcohol.Alcohol-induced asthma is related to the genotypes of ALDH2and is caused by rapid elevation of blood Ac-CHO. ALDH1 playsa very important role in the oxidation of Ac CHO in blood. 相似文献
16.
THE IMPACT OF DIABETES MELLITUS ON THE PROGNOSIS OF ALCOHOLICS 总被引:1,自引:0,他引:1
YOKOYAMA AKIRA; MATSUSHITA SACHIO; ISHII HIROMASA; TAKAGI TOSHIKAZU; MARUYAMA KATSUYA; TSUCHIYA MASAHARU 《Alcohol and alcoholism (Oxford, Oxfordshire)》1994,29(2):181-186
In this study, the mortality of clinically treated Japanesealcoholics with diabetes mellitus was analysed. Fifty-one diabeticalcoholics without liver cirrhosis (DM), 23 diabetic and cirrhoticalcoholics (DM LC), 44 cirrhotic alcoholics without diabetes(LC), and 354 alcoholics without either complication (AL) admittedto the National Institute on Alcoholism in 1985 were studied.Thirty-seven diabetics required insulin treatment, and 12 oralhypoglycemic agents. The 4.4-year survival and drinking statusafter discharge were studied in 1990. Stepwise logistic regressionanalysis showed that the estimated odds for death increased8.10, 4.38, 3.70, and 3.27 times for the subjects with the alcoholmisuse after discharge, DM, DM LC, and LC, respectively. The4.4-year survival rate of alcoholics who continued misusingalcohol was much lower in DM (26%, P < 0.0005) and LC (35%,P < 0.0001) than in AL (73%). The survival rate of thosewho stopped misusing alcohol was significantly higher in DM(90%, P < 0.0001), LC (88%, P < 0.0001) and AL (94%, P< 0.0005) than those who continued misusing alcohol. Therewas no significant difference in the survival rate between thealcoholics with DM LC who continued misusing alcohol (50%) andthose who stopped misusing alcohol (73%). In the dead patients,56% of DM died unexpectedly or suddenly, whereas 71% of LC diedof liver failure after hospitalization. These results suggestthat diabetic alcoholics should be intensively educated forabstinence. 相似文献
17.
A Comparison of Women and Men Undergoing Catheter Ablation for Sustained Monomorphic Ventricular Tachycardia
下载免费PDF全文
![点击此处可从《Journal of cardiovascular electrophysiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
18.
19.
Keratinocyte growth factor is an endogenous stimulant of rabbit gastric epithelial cell proliferation and migration in primary culture 总被引:2,自引:0,他引:2
MORIO TAKAHASHI SHINICHI OTA SHUJI NISHIMURA KEIJI OGURA SHIN MAEDA NOBUO TODA EIJI HAMADA AKIRA TERANO MASAO OMATA 《Journal of gastroenterology and hepatology》1996,11(11):1089-1096
Mesenchymal-epithelial interactions are important in the gastric mucosal repair. However, specific factors responsible for such interactions have not been established. In the present study, keratinocyte growth factor (KGF) significantly stimulated proliferation of gastric epithelial cells dose dependently and synergistically with hepatocyte growth factor (HGF), epidermal growth factor (EGF) and insulin. Restitution of gastric epithelial monolayers was also assessed, using a round wound restitution model. Keratinocyte growth factor facilitated the restitution of gastric epithelial cells significantly but did not have any effects on gastric fibroblasts. Keratinocyte growth factor receptor mRNA was expressed by gastric epithelial cells, indicating that these effects were elicited by the specific receptor mediated pathway. Northern blot analysis revealed the expression of KGF mRNA in gastric fibroblasts but not in gastric epithelial cells, indicating the production of KGF. These results suggest that KGF might be involved in gastric mucosal repair, through mesenchymal-epithelial interaction. 相似文献
20.
SHUICHIRO SHIINA YUTAKA KOMATSU TAKAO KAWABE KENTA YOSHIURA EIJI HAMADA YASUO HATA YASURO NIWA TAKAO KAWASE SHINICHI OTA YASUSHI SHIRATORI AKIRA TERANO TSUNEAKI SUGIMOTO 《Journal of gastroenterology and hepatology》1992,7(2):154-156
Cholestatic hepatocellular carcinoma, which grows into the bile duct and causes obstructive jaundice, is rare and difficult to diagnose. A case is presented in which cholestatic hepatocellular carcinoma was detected by deposit of Lipiodol. This is also the first case that was successfully treated by endoscopic retrograde biliary drainage and transcatheter arterial embolization. 相似文献