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排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
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R Mandeville F Dumas A Amarouch S Sidrac-Ghali M C Walker M Zelechowska I Ajdukovic B Grouix 《Hybridoma》1987,6(5):441-451
This study reports the purification and characterization of a high molecular weight human breast cancer-associated antigen identified by a previously described (1,2) murine monoclonal antibody, BCD-B4. Immunohistochemical analysis indicated that BCD-B4 recognizes an antigen expressed in an altered form on the human breast carcinoma cell line, BT-20, compared to the non-malignant human mammary epithelial cell line, HBL-100. Chemical treatments and enzymatic digestions suggested that the recognized moiety was a protein. The antigenic determinant was resistant to neuraminidase and periodate treatments but was sensitive to trypsin and proteinase K. The antigen was purified by affinity chromatography and its molecular weight, determined by SDS-PAGE analysis under non-reducing conditions, was proven to be 250 Kd. Under reducing conditions, the molecule dissociated into two polypeptides of 125 and 45 Kd, respectively. Both subunits could be isolated from normal HBL-100 and neoplastic BT-20 cellular protein extracts by affinity chromatography. The higher molecular weight subunit showed; however, qualitative and quantitative differences between the two cell lines: it was expressed in greater quantity on BT-20 cells and its molecular weight was 15 Kd higher. Both subunits could also be identified by immunoblots of BT-20 cells. 相似文献
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Physicians as gatekeepers. A complex challenge 总被引:1,自引:0,他引:1
M D Reagan 《The New England journal of medicine》1987,317(27):1731-1734
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F. Berndsen D. Arvidsson L.-K. Enander C.-E. Leijonmarck U. Wingren C. Rudberg S. Smedberg G. Wickbom A. Montgomery 《Hernia》2002,6(2):56-61
Interest in inguinal hernia surgery has increased significantly with the introduction of new operating techniques during
the past decade. This multicenter study compared short-term results in patients treated by the laparoscopic transabdominal
preperitoneal patch technique (TAPP; n=518) and the Shouldice technique (n=524). We evaluated demographics, operative data, complications, hospital stay, postoperative pain, use of analgesics, functional
status, sick leave, and complaints up to 3 months postoperatively. The median operating time was shorter in the Shouldice
group (55 vs. 65 min), but there were no significant differences in complication rates, and major complications were rare.
The hospital stay was 1 day or less in over 98% of cases in both groups, but more operations were performed on outpatient
basis in the Shouldice group. In the TAPP group postoperative pain and analgesic consumption were less, postoperative functional
status was better, and sick leave was shorter (10 vs. 14 days). These results show that the two methods are equally safe and
have few major complications. The TAPP operation is associated with less postoperative pain, better postoperative functional
status, and shorter sick leave, but at the price of a longer operating time.
Electronic Publication 相似文献
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