波形纤维蛋白在胎肺及肺腺癌的免疫组化表达

<正>取13至30周正常胎儿肺组织24例肺腺癌16例和新生儿肺8例,用三种抗中间丝蛋白(角蛋白Ker-atim、波形纤维蛋白Vimentin和结蛋白Desmin)单克隆抗体,经免疫组化ABC法进行实验观察.结果显示:胎肺发育分化阶段及肺腺癌组织中这些蛋白组分的表达均与正常成体细胞中的中间丝蛋白有高度特异性的特征绝然不同.胎肺13周首先出现角蛋白,15周出现波形纤维蛋白,16周才有结蛋白阳性表达,而且25周以前波形蛋白是最为反应活跃的蛋白组分.波形纤维蛋白除了在结缔组织、软骨、间充质等出现阳性反应并在上皮与角蛋白,在肌组织中与结蛋白表现出协同表达的特征.在16例肺腺癌组织中除角蛋白阳性表达以外,有     

胃镜活检诊断并经手术证实的胃原发性淋巴瘤

本文报道8例胃原发性恶性淋巴瘤并将胃镜活检与术后病理诊断相对比,提出胃原发性恶性淋巴瘤的临床及病理特征。8例淋巴瘤患者中,男性3例,女性5例,平均年龄为40.1岁。临床表现虽不特异,轻者仅为上腹部饱胀感,重者可出现恶心呕吐,但以腹部痛疼为常见症状。该瘤累及部位多在胃窦部,本组8例中7例累及胃窦。胃镜所见,常以大小不等的丘     

胎肝细胞质液对重症病毒性肝炎患者外周血TL－CFU及mIL－2R影响的实验观察

采用半固体一步单层琼脂培养法和单克隆荧光抗体技术分别观察重症肝炎外周血ＴＬ－ＣＦＵ和ｍＩＬ－２Ｒ，发现重症肝炎患者ＴＬ－ＣＦＵ（１０４．４±３２．６）及ｍＩＬ－２Ｒ（３５．６±８．６）较正常人明显降低。在培养体系中加胎肝细胞质液后，无论在重症肝炎组还是在正常组均不能明显地提高ＴＬ－ＣＦＵ，说明胎肝细胞质液不含具生物佐的促ＴＬ－ＣＦＵ因子。但对ｍＩＬ－２Ｒ表达的影响，在重症肝炎组病人，只有在ＰＨＡ存在条件下才能促进ｍＩＬ－２Ｒ的表达，说明胎肝细胞质波含有某种（些）物质能协同ＰＨＡ促进重症肝炎患者外周血淋巴细胞ｍＩＬ－２Ｒ表达。     

120例教师血液流变学观察分析

血液流变学的检测广泛应用于心脑血管病、外周血管病、血液系统病、代谢性疾病等的诊断、预防、治疗及群体普查。笔者对120例教师进行血液流变性检测观察 ,发现教师的血液黏度有显著增高 ,现将检测结果与分析报道如下。材料与方法1对象(1)本院120例参加体检的教师 ,其中男70例 ,年龄20～65岁 ,平均年龄35.3岁 ;女50例 ,年龄20～60岁 ,平均年龄34.5岁。(2)健康对照组117例 ,系我院健康体检者 ,无心脑血管病史、肝肾功能、血糖、血脂均正常 ,其中男65例 ,年龄19～64岁 ,平均年龄35.5岁 ;女52例 ,年龄20～61岁 ,平均年龄35.4岁。2方法早晨空腹…     

中国十一个少数民族的皮纹研究

<正> 本文是对多民族皮纹学研究,通过不同人群间相互比较分析,探索皮纹的遗传规律,并为研究人类微进化提供依据。用油量法分别印取指纹和掌纹样本,在放大镜下观测并纪录。观测分析主要根据Cnminns等人(1929)修订的方法以及国内通用方法和标     

Proteolysis-independent regulation of PI3K by Cbl-b-mediated ubiquitination in T cells

Cbl-b, a ring-type E3 ubiquitin protein ligase, is implicated in setting the threshold of T lymphocyte activation. The p85 regulatory subunit of phosphatidylinositol 3 kinase (PI3K) was identified as a substrate for Cbl-b. We have shown that Cbl-b negatively regulated p85 in a proteolysis-independent manner. Cbl-b is involved in the recruitment of p85 to CD28 and T cell antigen receptor zeta through its E3 ubiquitin ligase activity. The enhanced activation of Cbl-b(-/-) T cells was suppressed by the inhibition of PI3K. The results suggest a proteolysis-independent function for Cbl-b in the modification of protein recruitment.     

Persistence and amplification of St. Louis encephalitis virus in the Coachella Valley of California, 2000-2001

The introduction of a St. Louis encephalitis virus (SLE) genotype new to southeastern California during 2000 was followed by focal enzootic amplification in the Coachella Valley that was detected by seroconversions of 29 sentinel chickens in five of nine flocks of 10 chickens each, isolations of virus from 30 of 538 pools of 50 Culex tarsalis Coquillett females, and collection of 30 positive sera from 2,205 wild birds. This SLE strain over wintered successfully and then amplified during the summer of 2001, with 47 sentinel seroconversions in eight of nine flocks, 70 virus isolations from 719 pools of Cx. tarsalis and Cx. p. quinquefasciatus Say, and 40 positive sera from 847 wild birds. Human illness was not detected by passive case surveillance, despite issuance of a health alert during 2001. Virus amplification during both years was associated with above average temperatures conducive for extrinsic incubation and below average precipitation during spring associated with below average vector abundance. Seroconversions by sentinel chickens provided the timely detection of virus activity, with initial conversions detected before positive mosquito pools or wild bird infections. Vertical infection was not detected among Cx. tarsalis adults reared from immatures collected during the fall-winter of 2000, even though SLE over wintered successfully in this area. Early seroconversions by a sentinel chicken during February 2001 and a recaptured Gambel's quail in April 2001 provided evidence for transmission during winter and spring when ambient temperatures averaged below 17 degrees C, the threshold for SLE replication.     

乙型肝炎病毒正链转录及调节的研究进展

乙型肝炎病毒正链转录体(HBV asRNA),通过与HBV负链转录产生的mRNA互补结合成双链RNA(dsRNA),在核内或胞质中下调病毒的转录与表达,是病毒复制表达的重要自身调节分子。HBV通过抑制正链的转录,保护负链的转录产物作为逆转录及翻译相应病毒蛋白的模板,促进自身的复制与表达。因此,研究HBV正链的转录与调节,能深入对HBV分子病毒学机制的探索,为今后的抗病毒治疗提供新的思路。     

超声刀在腹腔镜下直肠癌根治术中的应用

自开展腹腔镜下右半结肠癌手术以来，超声刀及手术器械不断改进，腹腔镜技术逐渐成熟，使腹腔镜结直肠癌根治术更为安全。我院2006年5月至2007年8月应用超声刀在腹腔镜下行结直肠癌根治术14例，取得较好效果。现报告如下。     

Association of the CTLA-4 gene with rheumatoid arthritis in Chinese Han population

Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) is important for downregulation of T-cell activation, and CTLA-4 gene polymorphisms have been implicated as risk factors for rheumatoid arthritis (RA). Previous studies of the association between the +49 polymorphism of the CTLA-4 gene in RA have provided conflicting results. In order to determine association of the CTLA-4 gene with RA in Chinese Han population, we used denaturing gradient gel electrophoresis (DGGE) to genotype polymorphisms of four SNPs (MH30, +49, CT60 and JO31) of the CTLA-4 gene in 326 RA patients and 250 healthy controls. Furthermore, meta-analysis of all available studies relating +49 polymorphism to the risk of RA was performed to confirm the disease association. Among the SNPs examined, the genotype frequencies of CTLA-4 +49 and CT60 in RA patients differed significantly from controls (P=0.028 and 0.007). In addition, the distribution of four haplotypes constructed by these two SNPs was significantly different between patients and controls (chi(2)=10.58, d.f. =3, P=0.014). The meta-analysis also revealed that in both European and Asian populations, the CLTA-4 +49 G allele was associated with the risk of RA. These results suggested that the CTLA-4 gene might be involved in the susceptibility to RA in the Chinese Han population and both +49 and CT60 of CTLA-4 gene might be the causal variants in RA disease.