牙龈卟啉单胞菌对心血管内皮细胞黏附和入侵的研究

目的: 牙龈卟啉单胞菌(Porphyromonas gingivalis，P.g)作为牙周炎致病菌，同时也能引起心血管系统的疾病。在本研究中，比较4种P.g对人牙龈上皮细胞，人心血管内皮细胞，和人脐静脉内皮细胞的黏附和入侵。探讨P.g对人牙龈上皮细胞和心血管内皮细胞的黏附和入侵能力。方法: 通过抗菌素保护实验检测细菌对各种细胞的黏附和入侵能力。结果: 各种细菌的黏附和入侵能力是不同的，相同细菌对不同细胞的黏附和入侵也各有不同，P.g对人牙龈上皮细胞，人心血管内皮细胞的黏附和入侵明显高于对人脐静脉内皮细胞的黏附和入侵。结论: P.g菌株对牙龈上皮细胞和人心血管内皮细胞有着相似的黏附和入侵能力。     

兔动静脉短路环法诱导组织工程骨支架材料血管生成的初步研究

Objective To compare the effects of 2 vascular carriers, arteriovenous loop and arteri-ovenous bundle, on inducing angiogenesis in coral scaffold of vascularized tissue-engineered bone in animal models.Methods Thirty-six adult male New Zealand rabbits were randomized into 2 even groups.In group A, an arteriovenous loop (AVL) was formed by microsurgical anastomosis at the proximal ends between the femoral poptiteal artery and vein, and placed in the circular side groove of the coral block (6 mm × 8 mm × 10 mm) .In group B, flow-through vessels bundles of both femoral artery and vein were placed in the side grooves of the coral block.All the implants in 2 groups were wrapped by a micro-porous expand-ed-polytetrafluoroethylene (ePTFE) membrane, and fixed subcutaneously by suturing.Evaluation methods included gross morphological observations, histological examinations, India ink perfusion and vascular casting after 2, 4, 6 weeks.The density of blood vessels was analyzed by the statistical software SPSS 10.0.Results All the corals were encased by newly formed fibrovascular tissues in 2 groups.Ink-stained vessels distributed the surfaces and side grooves, and invaded the interspaces of corals.The degree of vascularization increased over the course of experiment.Blood vessel density demonstrated a significant continuous increase between 2 and 6 weeks after implantation in group A.The mean value of blood vessel density in group A (2 weeks 276.60±4.67, 4 weeks 517.20±10.66, 6 weeks 707.00 ±11.87) was significantly higher than in group B (2 weeks 153.60 ±7.16, 4 weeks 269.40±6.80, 6 weeks 279.20±6.53) (P <0.01).Vascular casting showed that in group A, significant blood vessels sprouted from all areas of the loop, espe-cially at the entrance of the arteriovenous pediele where the small tubes were densely interconnected.In group B, however, no blood vessels sprouted from the arteriovenous bundles and only some small vessels grew from the entrance and exit.Conclusions A vascularized coral model can be constructed by inserting an ar-teriovenous loop or an arteriovenous bundle, useful in vascular bone tissue engineering.The former, however, have stronger abilities to induce angiogenesis than the latter.     

新型三维钛网在眶底缺损重建整形手术中的应用

目的:介绍三维钛网在眶底缺损整复中的应用.方法:对4例因上颌骨鳞癌行上颌骨全切或扩大切除的患者以三维钛网行眶底及眶下缘的重建,评价术后眶部外形及眼的功能.结果:4例手术均获得满意效果,眶部外形恢复好,眶下区无明显塌陷,眼球运动好,无复视、感染等并发症.结论:采用新型三维钛网重建上颌骨肿瘤术后眶底及眶下缘缺损畸形能获得满意的眶部外形;防止术后复视;利于术后观察和复诊;术式简单、创伤小.三维钛网具有易成形、可塑性强、组织相容性好等特点.是理想的眶底重建整复材料,值得在临床推广.     

异种脱细胞真皮基质修复口腔颊部肿瘤切除后组织缺损的临床观察

目的:探讨异种脱细胞真皮在口腔颊部肿瘤切除后组织缺损修复中的应用方法、效果及长期观察的评价。方法:收集2006年4月～2009年5月口腔颊部肿瘤切除后组织缺损应用异种脱细胞真皮基质修复膜进行修复的19例患者资料。术后进行追踪随访和功能评价。结果:术后随访6～24个月,平均15个月。19例患者的组织缺损修复均获得成功,术后7～10天均未见修复膜脱落,愈合良好,植入异种生物膜部位逐渐为自体膜组织长入。长期观察,活化后的修复膜在软组织部位受植床存在一定的收缩。结论:生物膜修复组织缺损的机理与传统的植皮方法不同,是生物支架功能,异种脱细胞真皮基质适合口腔颊部肿瘤切除后浅、中层组织缺损的修复。     

对中美口腔医学本科课目大纲的比较分析

通过对中国口腔医学院校与美国牙学院校的教学大纲、课程设置、实习时间等的比较研究发现,中国口腔医学教育从各方面与美国比较既有共同点也有很大不同。借鉴国外的经验,提高我国口腔医学教育的水平,对提高我国口腔卫生健康水平有重要意义。     

Sonic hedgehog参与人胰岛素样生长因子-1信号通路促使下颌骨髁突过度生长的实验研究

目的:通过活体动物实验观察人胰岛素样生长因子-1(insulin-like growth factor,IGF-1) 与Sonic hedgehogxinh (Shh)介导的信号通路促进了髁突过度生长的作用。方法:制备IGF-1高表达表型转基因小鼠模型。将转基因鼠随机分为为5组:Cyclopamine刺激组;NVP-AEW541刺激组;IGF-1刺激组;Cyclopamine+IGF-1刺激组;生理盐水刺激组:空白对照,每组3只。随机选取一侧关节腔自31 d起隔天连续进行上述药物注射,共14 d,注射结束后收获实验侧髁突,固定,比较两侧髁突大小,分析差异性。结果:Cyclopamine刺激组及生理盐水刺激组实验侧与对照侧髁突大小比较差异无统计学意义。NVP-AEW541刺激组、IGF-1刺激组以及Cyclopamine+ IGF-1刺激组实验侧髁突小于对照侧髁突(P<0.05)。结论:IGF-1介导的信号通路促进了髁突肥大髁突的过度生长。     

脂肪细胞型脂肪酸结合蛋白与代谢综合征相关

Serum adipocyte fatty acid-binding protein (A-FABP) was raised in metabolic syndrome (MS)patients (n= 121) as compared with age-matched healthy subjects [n = 120, (14.7±4.8 vs 6.8 ±3. 0) μg/L,P<0.001]. It reached higher level in MS subjects with visceral obesity [(15.7±4.2 vs 12.6±5.1) μg/L, P<0.001]. Serum A-FABP concentration was positively correlated with body mass index, waist circumference, waist-tohip ratio, fasting insulin, homeostasis assessment for insulin resistance (HOMA-IR), fasting glucose, triglycerides,total cholesterol,and mean arterial blood pressure, whereas negatively correlated with HDL-C (r =-0. 448, P< 0.001).     

脂肪细胞型脂肪酸结合蛋白与代谢综合征相关

Serum adipocyte fatty acid-binding protein (A-FABP) was raised in metabolic syndrome (MS)patients (n= 121) as compared with age-matched healthy subjects [n = 120, (14.7±4.8 vs 6.8 ±3. 0) μg/L,P<0.001]. It reached higher level in MS subjects with visceral obesity [(15.7±4.2 vs 12.6±5.1) μg/L, P<0.001]. Serum A-FABP concentration was positively correlated with body mass index, waist circumference, waist-tohip ratio, fasting insulin, homeostasis assessment for insulin resistance (HOMA-IR), fasting glucose, triglycerides,total cholesterol,and mean arterial blood pressure, whereas negatively correlated with HDL-C (r =-0. 448, P< 0.001).     

下颌骨节段性缺损三种髂骨重建方法的疗效比较

下颌骨节段性缺损三种髂骨重建方法的疗效比较马秦毛天球刘宝林顾晓明下颌骨常因外伤、炎症、肿瘤切除术后遗留骨的部分缺损，尤其是节段性缺损，会引起不同程度功能丧失和面部畸形。近年来临床上应用自体髂骨修复下颌骨缺损的报道很多。本文对应用三种自体髂骨修复下颌骨...