脂肪细胞型脂肪酸结合蛋白与代谢综合征相关

Serum adipocyte fatty acid-binding protein (A-FABP) was raised in metabolic syndrome (MS)patients (n= 121) as compared with age-matched healthy subjects [n = 120, (14.7±4.8 vs 6.8 ±3. 0) μg/L,P<0.001]. It reached higher level in MS subjects with visceral obesity [(15.7±4.2 vs 12.6±5.1) μg/L, P<0.001]. Serum A-FABP concentration was positively correlated with body mass index, waist circumference, waist-tohip ratio, fasting insulin, homeostasis assessment for insulin resistance (HOMA-IR), fasting glucose, triglycerides,total cholesterol,and mean arterial blood pressure, whereas negatively correlated with HDL-C (r =-0. 448, P< 0.001).     

可吸收珊瑚／聚乳酸植骨材料的实验研究

目的：研究珊瑚／聚乳酸复合人工骨在体内、外的降解速度和力学性能。方法：测定珊瑚、聚乳酸和珊瑚／聚乳酸复合人工骨在１０ｍｌ、０．２ｍｏｌ／Ｌ缓冲液及小鼠背部皮下的降解吸收率,观察它们抗压强度的变化情况,统计学方法进行分析。结果：３种材料的体外降解吸收经无显著差异;珊瑚在体内的降解率与另两种材料同期比较,降解速度明显要快,存在显著差异;在植入前,两赫兹且间抗压强度存在显著差异,复合了聚乳酸的珊瑚抗压强     

兔动静脉短路环法诱导组织工程骨支架材料血管生成的初步研究

Objective To compare the effects of 2 vascular carriers, arteriovenous loop and arteri-ovenous bundle, on inducing angiogenesis in coral scaffold of vascularized tissue-engineered bone in animal models.Methods Thirty-six adult male New Zealand rabbits were randomized into 2 even groups.In group A, an arteriovenous loop (AVL) was formed by microsurgical anastomosis at the proximal ends between the femoral poptiteal artery and vein, and placed in the circular side groove of the coral block (6 mm × 8 mm × 10 mm) .In group B, flow-through vessels bundles of both femoral artery and vein were placed in the side grooves of the coral block.All the implants in 2 groups were wrapped by a micro-porous expand-ed-polytetrafluoroethylene (ePTFE) membrane, and fixed subcutaneously by suturing.Evaluation methods included gross morphological observations, histological examinations, India ink perfusion and vascular casting after 2, 4, 6 weeks.The density of blood vessels was analyzed by the statistical software SPSS 10.0.Results All the corals were encased by newly formed fibrovascular tissues in 2 groups.Ink-stained vessels distributed the surfaces and side grooves, and invaded the interspaces of corals.The degree of vascularization increased over the course of experiment.Blood vessel density demonstrated a significant continuous increase between 2 and 6 weeks after implantation in group A.The mean value of blood vessel density in group A (2 weeks 276.60±4.67, 4 weeks 517.20±10.66, 6 weeks 707.00 ±11.87) was significantly higher than in group B (2 weeks 153.60 ±7.16, 4 weeks 269.40±6.80, 6 weeks 279.20±6.53) (P <0.01).Vascular casting showed that in group A, significant blood vessels sprouted from all areas of the loop, espe-cially at the entrance of the arteriovenous pediele where the small tubes were densely interconnected.In group B, however, no blood vessels sprouted from the arteriovenous bundles and only some small vessels grew from the entrance and exit.Conclusions A vascularized coral model can be constructed by inserting an ar-teriovenous loop or an arteriovenous bundle, useful in vascular bone tissue engineering.The former, however, have stronger abilities to induce angiogenesis than the latter.     

不同区域颅底肿瘤的组织来源特点及手术方案的选择

目的:讨论不同区域颅底肿瘤的发生和组织来源的关系以及治疗方法的选择.方法: 收集232例与颅底解剖区域有关的良、恶性肿瘤病例资料,分析哪些组织来源的肿瘤易接近或易侵犯颅底的哪些区域,不同区域的肿瘤选择了何种手术进路和治疗方法.结果: 达颅前窝的肿瘤40例,其中上颌骨鳞癌23例、肉瘤7例、粘液瘤3例、造釉细胞瘤7例;达卵圆孔至茎突连线以外区域(包括颞下窝)70例,其中下颌骨升支部恶性肿瘤43例、腮腺恶性肿瘤复发者9例(腺样囊性癌4例)、耳颞区皮肤癌复发者3例、髁状突骨瘤及骨软骨瘤10例、神经来源肿瘤5例,达卵圆孔至茎突连线以内斜坡外侧区域114例,其中神经鞘瘤60例、脑膜瘤 9例、肉瘤 12例、低分化硬癌 4例、颈动脉体瘤16 例(指抵达颅底的部分病例)、腮腺来源肿瘤13例;枕骨大孔附近8例,其中脑膜瘤2例、脊膜瘤3例、神经鞘瘤3例.结论: 在能够接近或破坏颅底组织的肿瘤中发现,肿瘤组织来源不同,易发区域也有所不同.因此根据肿瘤发生的区域,可以初步判断肿瘤的组织来源,有利于诊断和鉴别诊断,同时可以帮助选择较为合理的手术进路.     

脂肪细胞型脂肪酸结合蛋白与代谢综合征相关

Serum adipocyte fatty acid-binding protein (A-FABP) was raised in metabolic syndrome (MS)patients (n= 121) as compared with age-matched healthy subjects [n = 120, (14.7±4.8 vs 6.8 ±3. 0) μg/L,P<0.001]. It reached higher level in MS subjects with visceral obesity [(15.7±4.2 vs 12.6±5.1) μg/L, P<0.001]. Serum A-FABP concentration was positively correlated with body mass index, waist circumference, waist-tohip ratio, fasting insulin, homeostasis assessment for insulin resistance (HOMA-IR), fasting glucose, triglycerides,total cholesterol,and mean arterial blood pressure, whereas negatively correlated with HDL-C (r =-0. 448, P< 0.001).     

BMP珊瑚植于下颌骨表面后的早期组织学表现

目的 研究复合BMP的珊瑚人工骨贴附移植于下颌骨表面后局部成骨及与下颌骨的结合情况。方法 12只杂种犬被随机分为4组，每组3只。将复合rhMP-2的珊瑚块植于下颌骨表面，用钛钉将其固定，令其与下颌骨表面尽量贴合。术后于不同时间点取材。制作组织学切片，光学显微镜观察。结果 1W，脱钙后局部残留大量空隙，材料周围包绕纤维结缔组织，组织内可见细胞增生聚集，间克质细胞增殖分化，大量炎细胞浸润。2W，材料内部细胞增生聚集更加明显，血管成分增多，可见新生骨样组织形成。3W，大量新生骨样组织形成，并有片状钙化的新骨沉积，编织骨形成。6W，珊瑚内大量成骨，新骨融合生长并改建成成熟的板层骨，与颌骨形成完全的骨界面。结论 复合BMP的珊瑚人工骨贴附移植于下颌骨表面后早期即可成骨，并可以与下颌骨形成骨结合。     

Experiment of non-vascularized iliac bone grafts with the simultaneous placement of titanium nnplants

AIM: To investigate the osseointegration process of titanium implant and non-vascularized iliac bone grafts. METHODS: 12 mongrel were divided into 4 groups randomly. Bone grafts were resected from iliac crest and then transplanted to the other side. animals were skilled in different time after surgery, X-ray pictures were taken, then histological observation were done. RESULTS: At 3rd week, bone grafts dissolved, resorbed or necrosis partly; At 6th week, new bone began to regenerated; At 9th week, the amount of new bone increased; At 12nd week, bone interface around implant formed, without soft tissue interrupt CONCLUSION: Osseointegration can formed between non-vascularized iliac bone grafts and titanium implant.     

透明质酸、TGF-β1对下颌骨髁突软骨增殖分化的影响

目的:研究透明质酸(HA)、TGF-β1因子对下颌骨髁突软骨增殖分化的影响.方法:取新生小鼠的下颌骨髁突软骨体外进行组织培养,按培养液内添加因子不同分为对照组、HA(0.5 mg/ml)、TGF-31(5 ng/ml)组,于培养1、2、4、6、8周后进行形态学观察、软骨面积测量、茜素红染色以及碱性磷酸酶染色研究.结果:对照组中髁突软骨在培养4周后软骨内开始出现高密度光阻射区,茜素红染色、碱性磷酸酶染色提示软骨基质出现了钙化、软骨内成骨的过程;HA组中髁突软骨内未出现高密度光阻射区,而髁突软骨面积却显著增大(P ＜0.05);TGF-β1组中髁突软骨在培养2周后提前出现了高密度光阻射区,然软骨面积无显著改变(P＞0.05).结论:在体外培养下,HA可以促进髁突软骨的增殖,对软骨细胞的肥大分化有一定的抑制作用,TGF-31在早期可显著促进髁突软骨细胞的肥大分化.     

下颌骨矢状劈开后退术对颞下颌关节的影响

目的:评价下颌骨矢状劈开后退术对颞下颌关节(TMJ)的影响.方法:研究我院50 例术前有颞下颌关节病(TMD),且行下颌骨矢状劈开后退术的患者,从关节压痛、弹响、张口度、张口型等方面量化评估术前、术后颞下颌关节症状变化情况.结果:从关节压痛、弹响、张口度、张口型等各方面评估均显示术后颞下颌关节症状评分均显著低于术前(P<0.05).结论:对于术前有颞下颌关节病的患者,行下颌骨矢状劈开后退术可以有效改善颞下颌关节症状.     

牙龈卟啉单胞菌红细胞凝集素A黏附和入侵人牙龈上皮细胞的研究

目的:检测牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)膜表面红细胞凝集素A(hemagglutinin A,HagA)黏附和入侵人牙龈上皮细胞的功能。方法:构建P.g381hagA基因的变异菌株,连接hagA基因到pYA292质粒上,并克隆到无毒性的沙门杆菌x4072菌中,通过比较变异菌株、野生菌株和表达hagA基因的沙门杆菌对人牙龈上皮细胞的黏附和入侵功能,检测HagA在此过程中的作用。结果:P.g381hagA基因变异菌株和野生菌株在对细胞的黏附和入侵过程中没有显著差别,但沙门杆菌x4072HagA表达菌株对细胞的黏附性和入侵性比对照组分别提高了3倍和4倍。结论:HagA参与了P.g381黏附和入侵牙龈上皮细胞的过程。