人晶状体上皮细胞HGF与c-Met的表达对增殖和凋亡的影响

目的：探讨肝细胞生长因子(hepatocyte growth factor,HGF)在人晶状体上皮细胞(lens epithelial cell,LEC)的表达及其对晶状体上皮细胞的促增殖和抑制凋亡的作用。方法：取原代培养人晶状体上皮细胞,应用RT-PCR,Western blot检测HGF,C-Met的mRNA及蛋白的表达,应用MTT法检测HGF对晶状体上皮细胞增殖的影响,Western-blot检测：Bcl-2的蛋白表达,以揭示其对晶状体上皮细胞凋亡的影响。结果：HGF和c-Met在人晶状体上皮细胞有表达,HGF有促进晶状体上皮细胞增殖的作用,并能抑制凋亡产生。结论：HGF参与人晶状体上皮细胞的增殖代谢过程,与晶状体上皮细胞的增殖相关。     

子痫前期子宫螺旋动脉血流与孕妇血清血管内皮生长因子表达相关研究

目的应用彩色多普勒超声技术检测子宫螺旋动脉,与孕妇血清血管内皮生长因子(VEGF)检测相结合,分析子痫前期子宫螺旋动脉血流动力学参数改变和其病情严重程度的关系。方法对30例子痫前期孕妇及38名健康孕妇,在未经任何药物治疗前,应用彩色多普勒超声技术检测子宫螺旋动脉,并对结果进行记录和比较。用酶联免疫吸附试验(ELISA)法测定血清VEGF含量。结果①子宫螺旋动脉血流动力学参数子痫前期组较对照组搏动指数(PI)值、阻力指数(RI)值、收缩期峰值流速与舒张末期血流速度之比值(S/D)增高,差异有统计学意义(P<0.01)。子痫前期重度组与轻度组比较各血流动力学参数值均增高,差异有统计学意义(P<0.01)。②血清VEGF含量子痫前期组较对照组降低,差异有统计学意义(P<0.01),子痫前期重度组低于轻度组,差异有统计学意义(P<0.01)。③子痫前期血清VEGF水平分别与子宫螺旋动脉血流动力学参数PI(r=-0.79,P<0.01)、RI(r=-0.78,P<0.01)、S/D(r=-0.83,P<0.01)呈显著负相关。结论①彩色多普勒超声技术检测子宫螺旋动脉血流可以预测子痫前期严重程度。②子痫前期孕妇血清VEGF表达与子宫螺旋动脉血流动力学参数之间呈显著负相关。     

负压泪小管钻切术的远期疗效观察

我们于1993—02～1996-10采用负压泪小管钻切术治疗非创伤性泪小管阻塞，疗效满意。现将随访观察时间超过2年的32例（35眼）报告如下。l材料与方法1．1临床资料采用本方法治疗的32例（35眼）患者中，男性13例（14眼），女性19例（ZI眼）。年龄32～65岁。溢泪史l～8年，均否认创伤史。术前经泪道冲洗及泪道探针探查证实泪小管阻塞，其中15例（16眼）经上泪小点冲洗泪道通畅。手术时，眼局部无活动性炎症。1．2手术方法采用l％ncain注入泪小管行表面麻醉。用泪点扩张器扩大下泪小点至lmrn，负压泪小管钻切：将自制的钻切针头与sml注射器相接…     

以现代战争为背景,强化卫生员训练实战功能

现代高技术条件下的局部战争对后勤保障的要求极高,后勤保障的质量将直接制约战争的进程和结局。军队卫生员培训必须以作战需求为牵引,以提高应急保障能力为前提,不断深化教学改革,努力强化培训功能,在保证专业技能培训质量的基础上,     

Analysis of TGFBI gene mutation in a Chinese family with atypical Reis-Bückler corneal dystrophy

Objective To identify the TGFBI gene mutation and the relationship between genotype and phenotype of a Chinese family with atypical Reis-Bückler corneal dystrophy (RBCD). Methods Four patients, two non-carrier relatives of the family were enrolled in the present study. In addition to ophthalmologic examinations, PCR amplification and DNA sequencing of exons 4, 11, 12, and 14 of the TGFBI gene were carried out. Exon 14 was also sequenced in 100 healthy controls. Results A G to A transition at eodon 623 in all affected members was identified. This mutation resulted in a substitution of glyeine (GGC) to aspartic acid (GAC) at the protein level. None of the healthy family members, or any of the 100 control subjects carried this mutation. Conclusion The G623D mutation of the TGFBI gene caused an atypical Reis-Buckler corneal dystrophy in this family. This mutation is reported in Chinese for the first time.