无继发感染的泛发型湿疹122例皮肤菌群的测定和药敏分析

目的无继发感染的泛发型湿疹患者皮损处进行皮肤菌群的测定和药敏分析。方法选取符合湿疹诊断标准的2008年1月—2009年8月的住院患者,并剔除继发感染的病例,取分泌物和/或皮屑,进行细菌培养、菌种鉴定和药敏试验。结果临床无继发感染的湿疹患者(122例)皮损总的金葡菌检出率为27.05%,细菌检出率为42.62%。除金葡菌以外的细菌中表皮葡萄球菌检出率为13.93%。其中耐甲氧西林的金葡菌为42.6%,金葡菌对万古霉素和替考拉宁的敏感性高均达到100%,其次为利福平、四环素、庆大霉素、,复方新诺明、氯霉素、环丙沙星,但对克林霉素、红霉素、青霉素的敏感性均低于40%。结论本试验显示不同地区菌群分布的情况存在一定的差异性,且药敏结果变化较大。     

天津市公安特警甲真菌病致病菌调查

甲真菌病是皮肤科的常见疾病,某些特殊人群的发病率显著高于正常人群,2009年4月—2010年12月我科对我市公安特警进行了甲真菌病筛查,并对病甲标本进行了真菌学检查和分析,现报道如下。     

扁平苔藓样角化病1例

临床资料患者男,69岁。因双耳廓、臀部出现斑块伴鳞屑1年就诊。患者1年前无意中发现骶尾处有一指甲大角化性丘疹,不痛不痒,未予重视,随后,皮损缓慢增大,且于坐骨处、两侧髋骨处、右大腿内侧及双耳廓陆续出现类似丘疹及斑块,上覆痂皮,无自行破溃及压痛,不伴发热、关节痛、淋巴结肿大等,无口腔黏膜破溃。     

整合素α6单克隆抗体对人乳头瘤病毒6/11病毒颗粒体外感染HaCaT细胞的影响

目的 探讨整合素α6单克隆抗体(GoH3)对人乳头瘤病毒(HPv)6/11病毒颗粒(VP)体外感染HaCaT细胞的影响.方法 ①采用4组不同浓度的HPV6/11 VP体外感染HaCaT细胞;②加入6组不同浓度的GoH3后,以106拷贝/ml HPV6/11 VP体外感染HaCaT细胞;③加入稀释倍数为1∶100的GoH...     

Influence of an anti-integrin α6 monoclonal antibody on the in vitro infection of human HaCaT keratinocytes with human papillomavirus (HPV) 6/11 virus particles

Objective To investigate the influence of an anti-integrin α6 monoclonal antibody (GoH3) on the in vitro infection of a human keratinocyte cell line HaCaT with HPV6/11 virus particles (VP). Methods HaCaT cells were infected in vitro with 4 different concentrations of HPV6/11 VP alone, HPV6/11 VP of 106 copies/ml after incubation with 6 different dilutions of GoH3, or 8 clinical isolates of HPV6/11 VP of 106 copies/ml before or after the incubation with 1∶ 100 dilution of GoH3. After additional culture, the infected HaCaT cells were collected and fluorescence quantitative (FQ)-PCR was performed to detect the HPV DNA load in cells. The inhibition rate of CoH3 on the infection was calculated. Results The viral load was different among the HaCaT cells infected with different concentrations of HPV6/11 VP (P < 0.01). The inhibition rate on the infection positively correlated with the concentration of CoH3 when the dilution was more than 1∶ 100; however, when the dilution was less than 1∶ 100, the increase in CoH3 concentration had no influence on the inhibition rate. The average viral load in HaCaT cells infected with clinical isolates of HPV6/11 VP was (5.81 ± 2.51) × 104 copies/ml in the absence of GoH3, (3.02 ± 1.21) × 104 copies/ml with the presence of CoH3, and the average inhibition rate of GoH3 was (46.9 ± 4.7)%. Conclusions GoH3 could partially suppress the adhesion of HPV6/11 VP to HaCaT cells, hinting that integrin a6 is an important HPV6/11 VP receptor on host cells.     

Influence of an anti-integrin α6 monoclonal antibody on the in vitro infection of human HaCaT keratinocytes with human papillomavirus (HPV) 6/11 virus particles

Objective To investigate the influence of an anti-integrin α6 monoclonal antibody (GoH3) on the in vitro infection of a human keratinocyte cell line HaCaT with HPV6/11 virus particles (VP). Methods HaCaT cells were infected in vitro with 4 different concentrations of HPV6/11 VP alone, HPV6/11 VP of 106 copies/ml after incubation with 6 different dilutions of GoH3, or 8 clinical isolates of HPV6/11 VP of 106 copies/ml before or after the incubation with 1∶ 100 dilution of GoH3. After additional culture, the infected HaCaT cells were collected and fluorescence quantitative (FQ)-PCR was performed to detect the HPV DNA load in cells. The inhibition rate of CoH3 on the infection was calculated. Results The viral load was different among the HaCaT cells infected with different concentrations of HPV6/11 VP (P < 0.01). The inhibition rate on the infection positively correlated with the concentration of CoH3 when the dilution was more than 1∶ 100; however, when the dilution was less than 1∶ 100, the increase in CoH3 concentration had no influence on the inhibition rate. The average viral load in HaCaT cells infected with clinical isolates of HPV6/11 VP was (5.81 ± 2.51) × 104 copies/ml in the absence of GoH3, (3.02 ± 1.21) × 104 copies/ml with the presence of CoH3, and the average inhibition rate of GoH3 was (46.9 ± 4.7)%. Conclusions GoH3 could partially suppress the adhesion of HPV6/11 VP to HaCaT cells, hinting that integrin a6 is an important HPV6/11 VP receptor on host cells.     

甲型H1N1流感病人的护理

[目的]探讨甲型H1N1流感的观察与护理。[方法]回顾性总结190例甲型H1N1流感病人的护理,即加强病区管理,做好标本采集、用药护理、病情观察、发热护理、心理护理及健康教育。[结果]190例病人病程1d～10d,均治愈出院,无死亡病例。[结论]掌握甲型H1N1流感发生发展规律,提供系统全面的护理,可有效预防甲型H1N1流感并发症的发生,提高治愈率。     

耐多药结核病患者焦虑抑郁与服药依从性的相关性研究

目的 探讨耐多药结核病（MDR-TB）患者焦虑抑郁与服药依从性的相关性。方法 选取2020年12月至2021年5月在深圳市第三人民医院就诊的91例耐多药结核病患者作为研究对象。使用医院焦虑抑郁量表测评患者的焦虑/抑郁得分情况,采用Morisky服药依从性量表测评患者的服药依从性得分情况,根据患者焦虑/抑郁得分情况分为焦虑/抑郁无症状、可疑症状、明显存在症状三组并使用方差分析比较三组不同焦虑/抑郁症状患者的服药依从性得分情况,使用Pearson线性相关分析探讨患者焦虑/抑郁与服药依从性的相关性。结果 MDR-TB患者服药依从性平均得分为（6.15±1.09）分,服药依从性好者有12例（13.2%）,中等者37例（40.7%）,差者42例（46.1%）;患者焦虑状况平均得分为（9.11±3.26）分,抑郁状况平均得分为（10.06±3.26）分。三组不同焦虑状况患者依从性得分比较,差异有统计学意义（P<0.05）;三组不同抑郁状况患者依从性得分比较,差异有统计学意义（P<0.05）。多重比较结果显示,三组不同焦虑/抑郁状况患者在依从性得分上两两均存在统计学差异（P<0.0...     

第7版《WHO儿童基本药物示范目录》在儿童医院的使用情况分析

目的:基于样本儿童医院药品使用情况,为制定中国儿童基本药物目录提供参考.方法:利用18家三级儿童专科医院的药品采购数据,比较分析第7版《WHO儿童基本药物示范目录》(EMLc)与样本儿童医院用药在品种和剂型上的异同,根据欧洲药品管理局推荐的儿童用药剂型,分析不同年龄儿童适宜用药剂型的分布情况.结果:儿童医院用药与《WH...     

我国儿童用药立法的必要性和可行性研究

目的:探讨我国儿童用药立法的必要性和可行性。方法:通过对儿童用药现状及存在的主要问题进行分析,论述立法的必要性;通过对现行儿童用药相关的立法基础进行分析,结合欧盟和美国的立法经验探讨立法可行性。结果:我国儿童用药保障体系存在的问题日益突出,但现有政策缺乏强制性,因此立法是系统解决儿童用药问题的客观需要。目前儿童用药立法所必需的现实基础、法律基础、先行探索和经验借鉴等基本条件也已比较成熟,立法具有可行性。结论:儿童用药立法必要且可行,因此应加快儿童用药立法。