腹主动脉灌注法分离胰腺星状细胞及其培养、鉴定

目的:通过腹主动脉插管灌注胶原酶分离胰腺星状细胞(pancreatic stellate cell,PSC),建立简单易行的PSC分离方法,并对PSC进行培养和鉴定.方法:大鼠腹主动脉插管灌注后,经胶原酶消化、Nycodenz密度梯度离心,分离得到PSC;通过观察细胞形态、胞质内脂滴和免疫细胞化学方法检测结蛋白(desmin)、神经胶质酸纤维蛋白(glial fibrillary acidic protein,GFAP)、α平滑肌肌动素(α-smooth muscle actin,α-SMA)的表达来鉴定PSC.结果:腹主动脉灌注法分离得到PSC,产率、活力和纯度分别为:(15.3±4.6)×103/g体重、(95.0±3.5)%、＞80%;培养24 h后大多数细胞已贴壁,呈星状或多角形,48 h后表达desmin、GFAP,并在96 h后表达α-SMA.结论:通过腹主动脉灌注法较容易分离得到PSC,其产率、活力和纯度较高,经鉴定后能够满足体外实验要求.     

肩膀疼了半年 “元凶”竟不是肩周炎

正半年前,姚女士开始觉得肩膀隐隐作痛,因为年逾50,她判断自己患上了"五十肩"。听人建议后,姚女士开始常常伸胳膊、拉吊环、甩手臂锻炼,希望能缓解肩周炎的症状。可是一段时间之后,她却觉得肩痛愈发严重,背部也有种牵扯般的疼痛感。尤其是晚上,躺在床上,疼痛加倍,折磨得姚女士难以入睡。在这之后,姚女士频繁求医,换了两家医院,做了多     

吉西他滨耐药人胰腺癌细胞株的建立及其与肿瘤干细胞的相关性研究

Objectives To establish a gemcitabine-resistant pancreatic cancer cell line SW1990/ GZ,and to explore the relationship between drug-resistant cell line SW1990/GZ and pancreatic cancer stem cell. Methods Gemcitabine-resistant pancreatic cancer cell line SW1990/GZ was obtained by treating parental cell line SW1990 in vitro with increasing dosage of gemcitabine in culture medium intermittently for 24 weeks. Stable cultures were obtained which were 77. 2-fold increased in resistance relative to parental cells. Gene expressions of ABCB1/MDR1, ABCC1/MRP and ABCG2/BCRP were determined by real-time PCR. Tumorigenic potential was performed by nude mice xenograft transplant experiments. Side population analysis and CD24CD44 positive cells explore were determined by flow cytometry to examine cancer stem cell proportion. Results Gemcitabine-resistant cell line SW1990/GZ underwent obvious morphological and functional changes. Compared with the parental cell line,SW1990/GZ cell was small and turned into round shape. SW1990/GZ had a higher gene expression level of ABCB1/MDR1, ABCC1/MRP and ABCG2/BCRP than SW1990(P <0. 01). Nude mice xenograft transplant experiments showed that only 1 x 105 SW1990/GZ cells were sufficient for tumor formation, whereas an injection of 1 x 105 SW1990 cells did not initiate tumors. Flow cytometry analysis showed that SP proportion in SW1990/GZ was (11.0±1.0)%, whereas in parental SW1990 it was ( 4. 6 ± 0. 9 ) % , CD44CD24 positive cells was (8. 73±0. 81) % in SW1990/GZ, whereas (1.1±0. 4)% in SW1990. Conclusions Gemcitabine-resistant cell line SW1990/GZ has a higher proportion of pancreatic cancer stem cells compared to its parental cell line SW1990. CD44 is mainly responsible for acquired drug resistance,which can be a potential target to overcome acquired drug resistance in pancreatic cancer.     

气大伤身是真的

<正>生气对很多人来说是家常便饭,经常有人说"气死我了"、"我快气炸了"、"太气人了"之类的话。不过,你可知道常常生气的后果吗?那可是大大的吓死人。比如,长期火爆脾气,经常处于发怒状态的人,大多数会造成秃顶;生气的程度轻一点的,则会在额头两侧形成双尖的M字形的微秃……这种外表上的痕迹算是轻的了。     

慢性胰腺炎诊断、分类、分级标准的回顾与思考

常见的致病因素、典型临床症状、影像学、病理组织学证据是慢性胰腺炎(chronic pancreatitis,CP)诊断的基本要素1-2.国际上,涉及到这些要素的具体诊断标准,以及用于判断疾病严重程度,进行分类分级、指导治疗、提示预后信息的分类标准则存在多种意见,极不统一.     

保留中段胰腺的胰腺切除术治疗胰腺神经内分泌肿瘤1例报道

病例资料患者,女,50岁,因＂体检发现胰腺占位3个月余＂入院。查体无特殊。CT检查示：胰头钩突及胰尾部各见一低密度灶,其内可见钙化,动脉期呈均匀明显增强（图1）,静脉期密度仍高于正常胰腺组织（图2）;胰颈及胰体部见数个类圆形低密度灶,增强未见强化。CA-199为46.07 KU/L。遂行保留幽门的胰十二指肠切除术（PPPD）加胰体尾切除术（保留脾脏及脾动静脉）。术中扪及胰头部一直径约4.0 cm大的质硬肿块,肿块周围可扪及小囊肿;     

胰腺坏死感染的临床和实验研究现状

<正>经过两个半世纪尤其是近10年来的研究,急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)治愈率已稳步上升,但病死率仍在20％～30％左右,目前主要死因为感染性胰腺坏死(infected pancreatic necrosis,IPN)及多脏器功能不全综合征(MODF)。由此可见,IPN是ANP致命性并发症及导致死亡的直接原因。因此,研究IPN的发生机制、诊断及防治,成为当今研究ANF,进一步提高其治愈率的重要课题,本文现就IPN的研究进展作一综述。     

囊性淋巴管瘤的CT诊断

目的　探讨CT对囊性淋巴管瘤的诊断价值。方法　搜集了经手术病理证实的 14例囊性淋巴管瘤 ,男 6例 ,女 8例 ,年龄1～ 5 7岁。所有病例均行CT扫描。回顾性分析CT表现并与手术病理对照。结果　根椐囊性淋巴管瘤的发生部位分为头颈 4例、躯干 3例、内脏 7例 ,除 1例伴出血者外 ,病灶密度均匀 ,CT值均类似于水 ,在± 10HU之间。病灶大小直径 5～ 15cm ,边界清楚 ,相邻的组织受压移位。病灶呈圆形或类圆形 ,囊内可有分隔 ,囊壁薄 ,可强化。所有病例病程 1～ 10年 ,平均 ( 4 .5± 1.6)年。除伴出血1例外 ,其余病例均无疼痛 ,3例稍有躯干不适。结论　CT可以清晰地了解囊性淋巴管瘤的发生部位以及病灶累及范围 ,对指导临床治疗有十分重要的价值