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61.
微生态学是人们从宏观生态学向微观层次进行研究而发展起来的,是研究微观生态平衡、生态失衡和生态调整的一门新兴科学.我国微生态学家李兰娟院士提出微生态的定义为研究正常微生物群与其宿主相互关系的生命科学分支[1].随着器官移植在世界范围内的广泛应用,移植器官与宿主微生态之间的平衡关系成为了关注的焦点.了解器官移植患者微生态的变化,并采取措施减少微生态的紊乱,促进新的微生态平衡的建立将有助于改善移植患者的预后.肝移植使宿主微生物面临了新的器官,改变了微生物生存的环境,本文就此时微生物如何改变及新移植的肝脏如何影响微生物作一综述. 相似文献
62.
目的 探讨胆囊癌组织中P53和P -gP的表达 ,与生物学行为的关系及二者之间的相互关系。方法 应用免疫组织化学SP法对41例胆囊癌组织中的P53和P-gP的表达情况进行检测。结果 胆囊癌组织P53和P-gP的阳性率分别为46.3% ,56.1%。P53和P-gP的表达与胆囊癌的组织类型 ,分化程度 ,浸润转移无相关性。结论 P53,P-gP在胆囊癌中有一定的表达。P-gP是胆囊癌耐药的重要因素之一。P53与P -gP的表达与胆囊癌的组织类型 ,分化程度 ,浸润转移无关。P53与P -gP在胆囊癌中的表达具有相关性 相似文献
63.
目的:观察长链多不饱和脂肪酸花生四烯酸(AA)和/或二十二碳六烯酸(DHA)对体外培养新生大鼠海马神经元脂肪酸构成的影响。方法:采用无血清培养液培养新生大鼠海马神经元,实验组分别在对照组的无血清培养液中添加4μmol/LAA,4μmol/LDHA或总浓度为4μmol/L、比例为1:2~16:1的AA和DHA。采用毛细管气相色谱法分析神经元中脂肪酸的构成。结果:培养液中AA和DHA总浓度为4μmol/L、比例为1:2~16:1,海马神经元中AA百分含量、AA/DHA比值与培养液中AA和DHA比例均呈正相关(P均<0.001)。结论:培养液中海马神经元中AA百分含量、AA/DHA比值与培养液中AA和DHA比例均呈正相关。 相似文献
64.
电针对大脑中动脉闭塞大鼠脑内神经元特异性烯醇化酶及胶质纤维酸性蛋白表达的影响 总被引:1,自引:0,他引:1
目的:研究电针治疗对脑梗死病灶周围及海马神经元特异性烯醇化酶(NSE)及胶质纤维酸性蛋白(GFAP)表达的影响。方法:采用易卒中型。肾性高血压大鼠(RHRSP),电凝法凝闭大脑中动脉(MCAO)。MCAO后给予电针治疗,在治疗5d、2周后分别处死,行免疫组化染色,计算NSE、GFAP的阳性单位值。结果:梗死边缘NSE表达在梗死5d时处于低水平,梗死2周组增高,电针治疗2周后海马的NSE表达明显增加;梗死5d时病灶周围GFAP阳性细胞数目增多,染色加深,胞体增大,突起增粗、增长,2周时变化更明显。电针治疗5d后,梗死边缘GFAP阳性单位值明显增高。结论:早期电针治疗能够提高海马区NSE及梗死灶周围GFAP的表达,促进了缺血后脑的可塑性变化,构成了脑梗死功能恢复的物质基础。 相似文献
65.
Objective To evaluate the efficacy and safety of tacrolimus exposure in stable liver transplant recipients converted from FK506 twice a day to Advagraf (tacrolimus extended-release capsules) once daily. Methods This was an open-label, random, control and multi-center study.Eligible patients were 19 to 70 years of age, 6 months post-transplant with stable renal and hepatic function and receiving stable doses of tacrolimus twice a day for 2 weeks prior to enrollment. There were 86 patients in the experimental group and the control group, separately. The average age of experimental group and control group was 46 ± 10 and 49 ± 9, respectively. Patients in experimental group received Advagraf, once daily, and the dose was adjusted according to the drug concentration,and the drug concentration was between 2 to 10 μg/L. The control group given tacrolimus, twice daily, and the drug concentration was between 2 to 10 μg/L. Results The incidence of acute rejection reaction was 1.20 % and 1.18 % respectively in experimental group and control group, and the 95 %confidence interval was -3.25% ~3.31 % and -3.26% ~ 3.34 %, individually. There was 1 case of acute rejection reaction in experimental group and control group, respectively. The patient and organ survival rate was 100%. Sixteen adverse events occurred in 15 patients (17.65 %) of the experimental group, and 10 adverse events occurred in 10 patients (11.63 %) of control group. Severe adverse events relating to the test drug in experimental group occurred in 4 patients (4. 71 %). and 2 patients (2. 33) in control group.Conclision Clinical trials indicated that Advagraf has efficacy and safety profiles similar to those of tacrolimus. The drug is safe and may improve patient compliance. 相似文献
66.
肝移植进展 在院庆60周年“院士讲学周”活动中的专题讲座 总被引:1,自引:1,他引:0
0 引言
肝移植手术的设想最早于1956年提出.在动物实验的基础上,1963年,美国Starzl施行了第1例人类肝移植.1989年澳大利亚Strong对1例先天胆道闭锁的患儿成功实施了世界上第1例成人对儿童左外测叶活体肚移植(living donor liver transplantation,LDLT). 相似文献
67.
肝细胞肝癌(简称肝癌)是肝移植的主要适应证之一,但肝移植术后肝癌的复发和转移严重制约了肝移植治疗的效果.如何降低移植术后肝癌的复发率和提高长期生存率成为各个移植中心非常关心的问题.对于肝癌肝移植受者的选择,世界上各个肝移植中心纷纷提出了各自的标准. 相似文献
68.
Hepatic ischemia-reperfusion (I/R) injury occurs in many clinical procedures, including liver transplantation, hepatic trauma, and liver resection. The molecular mechanisms responsible for hepatic I/R damage however remain unknown.
There is increasing evidence that sphingholipids, in particular ceramide, play a role in stress and death receptor-induced hepatocellular death, contributing to the progression of several liver diseases including liver I/R injury. In order to further define the role of sphingolipids in hepatic I/R, systemic analysis of sphingolipids after reperfusion is necessary. In the present study, we investigated the lipidomic changes of sphingolipids in a rat model of warm hepatic I/R injury, by delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry (DE MALDI-TOF-MS). The total amounts of ceramide and sphingomyelin and the intensity of most kinds of spingolipids, mainly sphingomyelin, significantly increased 1 hr post-reperfusion (p<0.05) and reached peaks at 6 hrs post-reperfusion (p<0.01) compared to controls. Six new forms of ceramide and sphingomyelins appeared 6 hrs post-reperfusion, (m/z) 537.8, 555.7, 567.7, 583.8, 683.5, 731.4. Interestingly, a ceramide- monohexoside (m/z) 804.4 (CMH[d18:1C22:1+Na]+) also increased post-reperfusion and correlated with extent of liver injury after reperfursion. Three main forms of sphingolipids, ceramide, sphingomyelin and ceramide- monohexoside, are related to hepatic I/R injury and provide a new perspective in understanding the mechanism(s) responsible for hepatic I/R injury. 相似文献
69.
Objective To evaluate the efficacy and safety of tacrolimus exposure in stable liver transplant recipients converted from FK506 twice a day to Advagraf (tacrolimus extended-release capsules) once daily. Methods This was an open-label, random, control and multi-center study.Eligible patients were 19 to 70 years of age, 6 months post-transplant with stable renal and hepatic function and receiving stable doses of tacrolimus twice a day for 2 weeks prior to enrollment. There were 86 patients in the experimental group and the control group, separately. The average age of experimental group and control group was 46 ± 10 and 49 ± 9, respectively. Patients in experimental group received Advagraf, once daily, and the dose was adjusted according to the drug concentration,and the drug concentration was between 2 to 10 μg/L. The control group given tacrolimus, twice daily, and the drug concentration was between 2 to 10 μg/L. Results The incidence of acute rejection reaction was 1.20 % and 1.18 % respectively in experimental group and control group, and the 95 %confidence interval was -3.25% ~3.31 % and -3.26% ~ 3.34 %, individually. There was 1 case of acute rejection reaction in experimental group and control group, respectively. The patient and organ survival rate was 100%. Sixteen adverse events occurred in 15 patients (17.65 %) of the experimental group, and 10 adverse events occurred in 10 patients (11.63 %) of control group. Severe adverse events relating to the test drug in experimental group occurred in 4 patients (4. 71 %). and 2 patients (2. 33) in control group.Conclision Clinical trials indicated that Advagraf has efficacy and safety profiles similar to those of tacrolimus. The drug is safe and may improve patient compliance. 相似文献
70.
一直以来,浙江大学医学院附属第一医院秉承“严谨求实、科教强院、规范服务、管理增效”的理念,力求不断进步,为成就“值得信赖的医院”而努力。近十年来,我院以科技创新提高医疗质量,在实施科教强院战略方面进行了较为成功的探索,尤其在医院信息化建设方面,基本实现“无纸”和“无片”化,促进了医疗质量和服务质量的提高,在省内乃至全国建立了良好的品牌和广泛的影响力。2008年,获得了“卫生部数字化试点示范单位”的荣誉称号,在卫生部公布的首批20家数字化试点示范医院中,我院是浙江省唯一获此殊荣的医院。 相似文献