塞来昔布对人鼻咽癌CNE-2细胞生长抑制及放射增敏研究

Objective To investigate the growth inhibition and radiosensitization of Celecoxib in hu-man nasopharyngeal carcinoma cell line CNE-2. Methods CNE-2 growth inhibition by Celecoxib was eval-uated by MTT method. Apoptosis-related changes in morphology were observed by transmission electron mi-croscopy (TEM). Cell cycle distribution and apoptosis rate were measured by flowcytometry (FCM). The ex-pression of COX-2 protein was observed by SP method after the treatment of Celecoxib. Cells were randomly planted into four groups: irradiation control(Ci), drug group(Cd), irradiation group(R), and Celecoxib plus irradiation group(D+R). Single irradiation of 2,4,6,8,and 10 Gy were administered for colonogenic assay. Cell cycle distribution and apoptosis rate were analyzed at 6 Gy irradiation. Results The growth of CNE-2 cell was inhibited by celecoxib in a dose-and time-dependent manner, the IC50 was 80 μmol/L After the treatment, cell ratio of GO and G, phases was increased (47.03±2.76 vs 56.17±1.95, t=4.68, P= 0.010), whereas the ratio of S and G2/M phases was decreased (33.07±1.86 vs 24.87±1.76, t=5.54, P = 0.010; 19.30±0.53: 17.73±0.83, t=2.75, P=0.050), and the apoptosis rate was increased (1.57±0.47:10.47±0.31, t = 27.39, P = 0.000) in a dose-dependent manner. Apoptosis with nuclear chromatin condensation, fragmentation and cell shrinkage was found by TEM. SP method showed that Celeib decreased COX-2 expression (17.48±0.34 vs 12.82±0.51,t=13.20,P =0.00). The sensitivity ratio(D0) was 1.15. FCM showed that the percentage of cells in G2/M phase was significanty more in R and D+R groups than in Ci and Cd groups (68.00±1.65,54.27±5.74,17.60±0.80,14.86±1.23, t=47.70,P=0.000; t=11.63, P=0.000), and also significantly different between R group and D + R group (t=3.99, P= 0.020). The apoptosis rate was higher in R and D + R groups than Ci and Cd groups(4.83±0.97,9.50± 1.35,1.33±0.86 and 2.28±0.42,t=4.67,P=0.010;t=8.81, P=0.000), D + R group than R group(t =4.85,P=0.010). Conclusions Celecoxib can markedly inhibit the growth and induce apoptosis in CNE-2 cells,which may depend on COX-2 pathway. Celeeoxib potently enhances the radiosensitivity of CNE-2 cells,which may due to the repair inhibit of radiation-induced DNA damage, inhibit of cell proliferation,and enhancement of cell apoptosis after irradiation.     

鼻咽癌干细胞研究进展

鼻咽癌(nasopharyngealcarcinoma,NPC)是我国南方及东南亚各国常见肿瘤,其发病率男性高达27.49/万,女性达10.51/万.即使在我国北方地区,鼻咽癌也属于头颈部恶性肿瘤之首位,严重威胁人民健康和生命.鼻咽癌的治疗主要以放射治疗为主.     

丝裂霉素C对增生性瘢痕成纤维细胞增殖的影响

目的 探讨丝裂霉素C(MMC)对增生性瘢痕成纤维细胞(FB)增殖的影响及机制,为增生性瘢痕的治疗提供理论依据.方法 用组织块法体外培养人增生性瘢痕FB,应用MTT方法、Western blot技术结合光密度扫描分析,观察MMC对增生性瘢痕FB增殖活性的影响及Smad7蛋白的表达情况.结果 在24、48、72 h这3个时间段内,随着培养时间的增加,细胞生长抑制率呈现明显的上升趋势,并且MMC浓度为2.5～200 μg/mL时,对细胞生长有明显的抑制效应,与对照组比较存在统计学差异(P＜0,05),显示明显的时间剂量依赖关系.经2.5～200μg/mL不同浓度的MMC作用增生性瘢痕FB24 h,Smad7蛋白表达量增加.结论 MMC对增生性瘢痕FB的增殖起到抑制作用,同时诱导Smad7蛋白的表达.     

利用磁频率交互抑制在VVI型起搏器更换时的应用

病例:男性,65岁,退休干部.因原发性扩张型心肌病,Ⅲ度房室传导阻滞、反复晕厥,于1980年安置国产埋藏式VVI 型心脏起搏器,导管电极经左侧头静脉插入右心室,起搏器埋藏于左上胸部.术后恢复良好,多次用胸壁皮肤刺激法了解自搏心律,其心室停搏时间可达8秒以上.6年间共更换同类型起搏器5台.1985年1月15日最后一次更换     

伊班膦酸钠治疗转移性骨肿瘤临床观察

骨是癌症常见的转移部位。Jaffe 1958年报道25％～80％的癌症患，尸解时发现骨转移。其中疼痛是转移性骨肿瘤的主要临床表现，也是疼痛的常见原因。临床上以乳腺癌、前列腺癌、肺癌、甲状腺癌、肾癌等实体肿瘤最易出现骨转移，占转移性骨肿瘤的80％。2002年3月至2003年9月，我院采用国产伊班膦酸钠(艾本)治疗转移性骨肿瘤患42例，效果显，现报告如下：     

丝裂霉素C对瘢痕疙瘩成纤维细胞TGF-β和Smads表达的影响

目的探讨丝裂霉素C(MMC)对瘢痕疙瘩成纤维细胞(KFB)转化生长因子β(TGF-β)/Smads通路作用及机制。方法采用不同浓度的MMC作用于体外培养的KFB,通过采用RT-PCR方法,检测MMC对体外培养的KFB TGF-β1mRNA表达的影响,采用Western blot技术检测MMC作用下体外培养KFB中Smad2/3、Smad4、Smad7蛋白的表达。结果 RT-PCR方法显示:MMC干预组TGF-β1mRNA相对表达量明显低于正常对照组,从12.5μg/L MMC开始具有显著的统计学差异(P<0.05),随MMC浓度增大,MMC干预组TGF-β1mRNA相对表达量呈逐渐降低趋势。Western blot技术检测显示:12.5μg/LMMC开始对KFB中Smad7蛋白的表达具有明显增强效应(P<0.05),50μg/L MMC对Smad2/3蛋白的表达开始有减弱效应(P<0.05),而对Smad4蛋白的表达无明显变化。结论 MMC对TGF-β1mRNA的表达具有明显减弱效应;加入不同浓度的MMC能明显增加KFB中Smad7蛋白的表达,减弱KFB中Smad2/3蛋白的表达,而对Smad4蛋白的表达无变化。     

鞍区原发性绒毛膜上皮癌1例

患者女,16岁。因多饮、多尿10个月,伴停经、视力下降5个月于2009年2月就诊于当地医院,考虑"抑郁症",未行特殊处理;后出现进行性视力下降,2009年3月转我院行CT检查,提示鞍区占位,遂转入武汉同济医院治疗;2009年3月4日同济医院鞍区MRI提示鞍区占位性病变(图1),大小约2.5 cm×3.2 cm,考虑为垂体瘤或颅     

肺腺癌腹膜转移1例并文献复习

肺癌是世界范围内发病率及死亡率最高的肿瘤之一,其中非小细胞肺癌(non-small cell lung cancer , NSCLC)约占80％[1].相对于小细胞肺癌,NSCLC的治疗手段可选择性相对较多(手术、化疗、放疗、靶向、免疫) ,使得 NSCLC 患者的总生存期(overall survival ,OS)及无疾病进展生存期(progression-free survival ,PFS )明显延长.但是,仍有一部分患者出现远处转移从而影响预后.现报道1例肺腺癌广泛腹膜转移(peritoneal carcinomatosis ,PC)病例.