一个遗传三代的t(5;8)(p15;p21)家系报告

在遗传咨询门诊中发现一对夫妇中的女方有5号和8号染色体异常,为t(5;8)(P15;P21)平衡易位携带者,文献未见报告。经家系调查,12人中5人具有此易位染色体。现报告如下: 方法和结果对先证者及家系主要成员进行外周血常规培养,制片,G显带。每例计数30个分裂     

夏花蜂贝汤联用化疗治疗颈淋巴结转移癌的临床观察

目的观察夏花蜂贝汤联用化疗治疗颈淋巴结转移癌的疗效和不良反应。方法将70例病人随机分为两组,对照组予单纯化疗,综合组予夏花蜂贝汤联用化疗治疗,比较两组近期疗效、肿瘤进展时间(TTP)、化疗通过率、毒副反应、生活质量(QOL)。结果综合组客观有效率(RR)与对照组相仿,肿瘤进展时间(TTP)、化疗通过率、G3/4血液学毒性、周围神经毒性、生活质量(QOL)等方面明显优于对照组,差异有统计学意义(P值均小于0.05)。结论自拟夏花蜂贝汤与化疗药物联用可以改善和稳定颈淋巴结转移癌患者的生活质量,一定程度上降低化疗的毒副反应。     

塞来昔布对人鼻咽癌CNE-2细胞生长抑制及放射增敏研究

Objective To investigate the growth inhibition and radiosensitization of Celecoxib in hu-man nasopharyngeal carcinoma cell line CNE-2. Methods CNE-2 growth inhibition by Celecoxib was eval-uated by MTT method. Apoptosis-related changes in morphology were observed by transmission electron mi-croscopy (TEM). Cell cycle distribution and apoptosis rate were measured by flowcytometry (FCM). The ex-pression of COX-2 protein was observed by SP method after the treatment of Celecoxib. Cells were randomly planted into four groups: irradiation control(Ci), drug group(Cd), irradiation group(R), and Celecoxib plus irradiation group(D+R). Single irradiation of 2,4,6,8,and 10 Gy were administered for colonogenic assay. Cell cycle distribution and apoptosis rate were analyzed at 6 Gy irradiation. Results The growth of CNE-2 cell was inhibited by celecoxib in a dose-and time-dependent manner, the IC50 was 80 μmol/L After the treatment, cell ratio of GO and G, phases was increased (47.03±2.76 vs 56.17±1.95, t=4.68, P= 0.010), whereas the ratio of S and G2/M phases was decreased (33.07±1.86 vs 24.87±1.76, t=5.54, P = 0.010; 19.30±0.53: 17.73±0.83, t=2.75, P=0.050), and the apoptosis rate was increased (1.57±0.47:10.47±0.31, t = 27.39, P = 0.000) in a dose-dependent manner. Apoptosis with nuclear chromatin condensation, fragmentation and cell shrinkage was found by TEM. SP method showed that Celeib decreased COX-2 expression (17.48±0.34 vs 12.82±0.51,t=13.20,P =0.00). The sensitivity ratio(D0) was 1.15. FCM showed that the percentage of cells in G2/M phase was significanty more in R and D+R groups than in Ci and Cd groups (68.00±1.65,54.27±5.74,17.60±0.80,14.86±1.23, t=47.70,P=0.000; t=11.63, P=0.000), and also significantly different between R group and D + R group (t=3.99, P= 0.020). The apoptosis rate was higher in R and D + R groups than Ci and Cd groups(4.83±0.97,9.50± 1.35,1.33±0.86 and 2.28±0.42,t=4.67,P=0.010;t=8.81, P=0.000), D + R group than R group(t =4.85,P=0.010). Conclusions Celecoxib can markedly inhibit the growth and induce apoptosis in CNE-2 cells,which may depend on COX-2 pathway. Celeeoxib potently enhances the radiosensitivity of CNE-2 cells,which may due to the repair inhibit of radiation-induced DNA damage, inhibit of cell proliferation,and enhancement of cell apoptosis after irradiation.     

塞来昔布对人鼻咽癌CNE-2细胞生长抑制及放射增敏研究

Objective To investigate the growth inhibition and radiosensitization of Celecoxib in hu-man nasopharyngeal carcinoma cell line CNE-2. Methods CNE-2 growth inhibition by Celecoxib was eval-uated by MTT method. Apoptosis-related changes in morphology were observed by transmission electron mi-croscopy (TEM). Cell cycle distribution and apoptosis rate were measured by flowcytometry (FCM). The ex-pression of COX-2 protein was observed by SP method after the treatment of Celecoxib. Cells were randomly planted into four groups: irradiation control(Ci), drug group(Cd), irradiation group(R), and Celecoxib plus irradiation group(D+R). Single irradiation of 2,4,6,8,and 10 Gy were administered for colonogenic assay. Cell cycle distribution and apoptosis rate were analyzed at 6 Gy irradiation. Results The growth of CNE-2 cell was inhibited by celecoxib in a dose-and time-dependent manner, the IC50 was 80 μmol/L After the treatment, cell ratio of GO and G, phases was increased (47.03±2.76 vs 56.17±1.95, t=4.68, P= 0.010), whereas the ratio of S and G2/M phases was decreased (33.07±1.86 vs 24.87±1.76, t=5.54, P = 0.010; 19.30±0.53: 17.73±0.83, t=2.75, P=0.050), and the apoptosis rate was increased (1.57±0.47:10.47±0.31, t = 27.39, P = 0.000) in a dose-dependent manner. Apoptosis with nuclear chromatin condensation, fragmentation and cell shrinkage was found by TEM. SP method showed that Celeib decreased COX-2 expression (17.48±0.34 vs 12.82±0.51,t=13.20,P =0.00). The sensitivity ratio(D0) was 1.15. FCM showed that the percentage of cells in G2/M phase was significanty more in R and D+R groups than in Ci and Cd groups (68.00±1.65,54.27±5.74,17.60±0.80,14.86±1.23, t=47.70,P=0.000; t=11.63, P=0.000), and also significantly different between R group and D + R group (t=3.99, P= 0.020). The apoptosis rate was higher in R and D + R groups than Ci and Cd groups(4.83±0.97,9.50± 1.35,1.33±0.86 and 2.28±0.42,t=4.67,P=0.010;t=8.81, P=0.000), D + R group than R group(t =4.85,P=0.010). Conclusions Celecoxib can markedly inhibit the growth and induce apoptosis in CNE-2 cells,which may depend on COX-2 pathway. Celeeoxib potently enhances the radiosensitivity of CNE-2 cells,which may due to the repair inhibit of radiation-induced DNA damage, inhibit of cell proliferation,and enhancement of cell apoptosis after irradiation.     

环氧化酶-2对鼻咽癌血管生成及预后的影响

Objective： The purpose of this study was to evaluate cyclooxygenase-2 （COX-2） expression in nasopharyngeal carcinoma （NPC） and its correlation with clinicopathologic features, angiogenesis, and prognosis. Methods： The expressions of COX-2 and vascular endothelial growth factor （VEGF） and microvascular density （MVD） were determined with immunohistochemical methods in eighty-six NPC patients followed up over 5 years. Results： Sixty-three tumors （73.3%） were classified as COX-2 positive. COX-2 expression was positively related to VEGF expression （r=0.438, P〈0.01） and correlated with the tumor pathological grade, extent of primary lesion, lymph node metastasis, distant metastasis and shorter survival. Conclusion： Our results suggest that COX-2, being highly expressed and strongly correlated with angiogenesis in nasopharyngeal carcinoma, is apt to be used as a predictor of prognosis, including local recurrence and distant metastasis.     

肺癌血浆、瘤组织中内皮素表达及其临床意义

 目的　探讨内皮素 (endothelin ,ET)在肺癌患者血浆、瘤组织中的表达特征及其与病理、临床各因素之间的关系。方法　应用放射免疫法、免疫组化和图像分析技术 ,检测了 6 6例肺癌患者血浆和石蜡标本中ET表达 ,并将所有病例随访至 2年以上。结果　肺癌血浆ET含量明显高于正常对照组 (P <0 .0 1) ,瘤组织中ET阳性表达率高达 73% ,其含量显著高于支气管良性病变 (P <0 .0 1) ,并且分期愈晚、分化愈差、转移范围愈广 ,血浆和瘤组织中ET水平愈高 ,生存率愈低。结论　ET可作为判断肺癌预后的一项有价值指标 ,血浆和瘤组织中ET表达具有一致性 ,在肺癌临床监测中具有同等价值。     

保尔佳在复发性非霍奇金淋巴瘤再化疗中使用效果的研究

探讨保尔佳在复发性中、高度恶性非霍奇金淋巴瘤再化疗中的使用效果。选取有明确病理诊断且既往用过ＣＨＯＰ方案化疗的复发性非霍奇金淋巴瘤共４８例，随机分为两组，治疗组再次启用ＣＨＯＰ方案化疗，并在化疗前５天开始加用保尔佳针剂３０μｇ肌肉注射，１次／日×２０日，对照组应用ＰｒｏＭＡＣＥ－ＣｙｔａＢＯＭ方案，分别评定近期疗效、毒副反应、Ｔ细胞亚群分布状态。治疗组ＣＲ率达３７．５％，对照组为５０．０％，差异无显著性（Ｐ＞０．０５）；两组共有的毒副反应为骨髓抑制、消化道反应及心肌毒性，均能耐受。但治疗组Ⅲ度以上消化道反应低于对照组（Ｐ＜０．０５）；治疗组化疗后Ｔ细胞亚群中ＣＤ＋３、ＣＤ＋４水平明显高于对照组（Ｐ＜０．０１）。保尔佳在复发的ＮＨＬ再化疗中有明显的增敏作用，且能提高机体的细胞免疫功能     

高聚金葡素联合化疗治疗恶性肿瘤

我科于 1998年 12月至 2 0 0 1年 4月间观察 2 0例全身化疗联合高聚金葡素 (ＨＡＳ)治疗恶性肿瘤取得较好的疗效。临床资料　 2 0例患者均有病理学或细胞学诊断证实为恶性肿瘤 ,具有全身化疗指征 ,无肝肾功能及造血系统损害 ,卡氏评分 70～ 90分。其中肺癌 8例 ,胃癌 6例 ,食管癌 4例 ,乳腺癌1例 ,白血病 1例 ;男性 16例 ,女性 4例 ,年龄 3 2～ 70岁 ,预期生存 >3个月 ,同种疾病及个人方案固定 ,具有良好的可比性。　附表　白细胞下降情况 (各组ｎ =2 0 )白细胞下程程度× 1 0 9/ＬＡ组例数 (% )Ｂ组例数 (% )4 .0～ 3 .0 6例 (30 % ) 4例 (…     

多学科团队模式在居家老年糖尿病患者血糖管理中的应用

目的探讨多学科团队模式对居家老年糖尿病患者进行血糖管理的应用效果。方法选取60例居家老年2型糖尿病患者为研究对象,对照组患者按照出院后常规随访管理,干预组在常规随访基础上按照多学科团队模式干预。选择糖化血红蛋白、空腹血糖、餐后两小时血糖、老年抑郁量表(GDS)、自我管理行为量表(SDSCA)作为评价指标。结果 (1)干预组空腹血糖、餐后2小时血糖和糖化血红蛋白均低于对照组,差异具有显著性(P0.05);(2)干预组抑郁得分低于对照组,差异具有显著性(P0.05);(3)干预组自我管理行为得分总分以及足部护理、正确服药方面得分差异有显著性(P0.05);饮食控制、运动管理及血糖监测方面无显著性差异(P0.05)。结论多学科团队干预模式可改善居家老年糖尿病患者的血糖控制水平,维护心理健康,能够在一定程度上提高自我管理能力。