不同方法对家蝇抗菌物质诱导的比较

目的比较不同方法对家蝇幼虫抗菌物质的诱导效果。方法室内饲养的家蝇三龄幼虫,分别经针刺诱导、沾取金黄葡萄球菌针刺诱导、沾取溶壁微球菌针刺诱导处理24 h和48 h,提取血淋巴,做抑菌试验,观察对溶壁微球菌和金黄葡萄球菌的抑菌效果。结果各诱导组与对照组之间抑菌环直径差异均有统计学意义(P<0.01),对溶壁微球菌的抑菌环直径明显大于对金黄葡萄球菌的抑菌环直径。3种处理方法之间比较,针刺诱导组处理后48 h提取的血淋巴对金黄葡萄球菌的抑菌环直径与金黄葡萄球菌诱导组和溶壁微球菌诱导组的差异有统计学意义(P<0.05),其他各组间差异均无统计学意义(P>0.05)。结论 3种诱导方法均能诱导家蝇幼虫体内抗菌物质的表达,表达的抗菌物质对溶壁微球菌的抑菌活性强于对金黄葡萄球菌的抑菌活性,抗菌物质对诱导方法可能无特异性。     

家蝇室内种群产卵动态观察

目的观察家蝇室内种群在整个产卵期内的产卵量动态变化规律。方法观察家蝇室内种群第3代及第4代成虫整个产卵期内的每天的产卵量,并进行分析。结果第3代成虫于产卵期第7天达产卵高峰,前10天产卵量相对较高,产卵期后期产卵量稳定在较低水平;第4代成虫于产卵期第4天出现产卵高峰,前7天产卵较多,后期产卵相对较少。结论家蝇室内种群产卵主要集中在产卵期前期,后期产卵能力较低,产卵量较少。     

丝光绿蝇室内种群孵化动态观察

目的观察丝光绿蝇室内种群的孵化特征。方法每天观察丝光绿蝇室内种群第7代及第8代幼虫孵化数量,未孵出数量,计算每天的孵化率,并进行分析。结果在产卵期前期,孵出的第7代及第8代幼虫数量相对较多,孵化率相对较高且稳定。后期孵出的幼虫数量相对较少,孵化率较低。结论丝光绿蝇室内种群产卵前期幼虫孵化率较高且稳定,转种应在前期进行。     

蜂胶对金黄色葡萄球菌的抑菌作用

[目的]了解产自河南的蜂胶对金黄色葡萄球菌的抑菌作用.[方法]采用琼脂平板扩散法,将实验分为8个不同pH组（蜂胶浓度31%）,以及在pH 6.5下设置10个不同浓度组即将31%蜂胶溶液倍比稀释至0.0621%（1：512）及空白组（0%）,每组3次重复,每张滤纸片（直径6 mm）滴加蜂胶溶液7μl,记录蜂胶对金黄色葡萄球菌24h的抑菌效果.[结果]随pH增加,河南蜂胶对金黄色葡萄球菌的抑菌活性呈阶段性减弱.随浓度降低,对金黄色葡萄球菌的抑菌活力减弱,在pH 6.5下,产生抑菌环的最小浓度为0.1242%（即实验剂量为8.47μg）.[结论]河南蜂胶对金黄色葡萄球菌有明显的抑菌活性.     

两组丝光绿蝇室内连续5代成虫形态观察比较

目的　观察比较两种饲料对丝光绿蝇室内连续饲养的各代成虫形态的影响。　方法　将丝光绿蝇引入室内 ,分成两组 ,即鱼粉组和麦麸组 ,分别采用两种幼虫饲料在室内进行连续饲养 ,分别随机测量两组室内第 1～ 5代成虫头宽 ,进行比较观察。　结果　成虫头宽经 t检验 ,组间比较 ,第 1、4、5代成虫头宽两组间雄性以及雌性均无显著性差异 ,第 2、3代鱼粉组成虫头宽显著性大于麦麸组 ;组内比较 ,两组内雄性以及雌性成虫头宽第 1代均显著性大于其第 2～ 5代。　结论　可能提示幼虫饲料添加鱼粉有利于丝光绿蝇的生长发育 ,将丝光绿蝇引入室内连续饲养 ,成虫形态有减小的趋势。     

Toll样受体与疟原虫侵染关系的研究进展

为深入了解疟原虫在侵染、繁殖过程中的分子机制,本综述从Toll样受体(Toll like receptor,TLR)与疟原虫的相互作用关系入手,探讨疟原虫在突破机体防御屏障后如何被TLR识别,并启动下游炎症反应的机制。其中涉及到的部分重要结构保守的生物大分子有成为新型药物靶分子的潜在特性。     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

河南蜂胶对溶壁微球菌的抑菌作用

目的探讨河南蜂胶对溶壁微球菌的抑菌效果.方法采用琼脂平板扩散法,以溶壁微球菌为实验菌种,设置不同的蜂胶稀释度组,每组3次重复,记录不同稀释度的蜂胶对溶壁微球菌24 h的抑菌效果.结果河南蜂胶原液从31.0%倍比稀释至1:512,9个稀释处理组对溶壁微球菌24 h的抑菌环直径依次分别为:(22.83±3.51)、(17.67±2.08)、(16.33±1.44)、(16.75±1.06)、(12.67±2.89)、(9.67±1.15)、(7.83±1.44)mm、0、0.结论河南蜂胶稀释度降低,抑菌环直径变小,最小可观察到抑菌环的稀释度为1:128.     

丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.