间期荧光原位杂交技术检测人/山羊嵌合模型

目的：建立一种高度灵敏特异的间期灾光原位杂交（interphase fluorescence in situ hybridization,IFISH）技术，以检测人／山羊嵌合模型中低比例的人细胞。方法：采用人特异Y染色体卫星DNA探针CEPY和17号染色体卫星DNA探针p17H8对两头移植人男性造血干细胞（hematopoietic stem cell,HSC）的人／山羊嵌合模型、1头正常阴性山羊和1名正常人男性的外周血进行IFISH分析。为评价FISH检测效率的正确性，将正常人男性与正常阴性山羊细胞按1／100、1／500和1／1000混合进行IFISH分析。并确定一套信号计数规则，以保证方法稳定可靠。结果：人／山羊混合细胞中人细胞阳性率与原比例相近。人细胞阳性率平均值为正常人男性98．60％（CEPY）和100．00％（p17H8）、正常阴性山羊为0、人／山羊嵌合模型为0．23％（CEPY）和0．11％（p17H8）。结果表明人／山羊嵌合模型中有低比例的人细胞存在，且为男性细胞。结论：本研究建立的IFISH技术高度灵敏特异，能真实反映检测细胞的比较，为人／山羊嵌合模型中低比例的人细胞检测提供了直观、灵敏、特异的途径。     

3种腋臭治疗方法的护理

腋臭是整形外科门诊常见病,我国发病率达6.41%,一般认为与大汗腺异常分泌有关,虽然不影响病人的身体健康,但身体明显的异味,常给病人造成心理压力,影响日常社交及生活[1].我科2006年9月-2009年4月共诊治腋臭病人350例,分别采用腋窝皱襞小切口大汗腺切除、肿胀麻醉下大汗腺吸刮治疗、腋窝A型肉毒毒素局部注射治疗3种方法,术后行3个月～24个月随访及护理,现回顾总结如下.     

不同扩增方法和探针长度对植入前单个卵裂球比较基因组杂交检测结果的影响

Objective To investigate the effect of different amplification methods and probes with various length on the results of comparative genomic hybridization (CGH) analysis of pre-implanted single blastomere and to establish the basis for preimplantation genetic diagnosis.Methods Twenty blastomeres of embryo at 6-8 cells stage were randomly divided into A and B group with 10 in each.Twenty peripheral blood lymphocytes from a healthy man were similarly divided into C and D group with 10 in each.Degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR) was used to amplify whole genomic DNA in group A and C,and multiple displacement amplification (MDA) was used in group B and D for whole genome amplification (WGA).The specificity of resultant products was confirmed by amplification of TBX1 gene exon 2.CGH was performed respectively with 250-750 bp and 750-2000 bp probes prepared from the amplified whole genomic DNA.The result of CGH was verified by sex-determining region of Y (SRY).Results (1) Nine of the 10 samples in group A and all in group C were amplifiable by DOP-PCR,but there were multiple non-specific bands in the amplification of TBX1 exon 2 when WGA products were used as templates.When 250～750 bp probe was used in CGH,1 of the 5 blastomeres was failed and another one had different karyotype from that analyzed by SRY.(2) All samples in group B and D were successfully amplified by MDA,and the non-specific bands were significantly less in the amplification of TBX1 exon 2.All 5 blastomeres were successful in CGH with the 250～750 bp probe.Moreover,the karyotype was in agreement with that of SRY.(3) When 750 ～ 2000 bp probe was used,the CGH results were suboptimal.Conclusions In WGA of single blastomere,MDA is superior to DOP-PCR in the stability and specificity.The karyotype image detected by CGH with the 250～750 bp probe is clear and homogenous.     

腰椎间盘突出症手术病人的术后健康教育路径

腰椎间盘突出症是骨科常见病和多发病,是腰腿痛最常见的原因。病人痛苦,严重者可致大小便功能障碍甚至截瘫。手术治疗是其主要手段之一,手术后卧床时间长,易发生严重并发症以至终生残废。健康教育路径是为满足病人对健康教育需求,依据标准健康教育计划为某一类疾病患者制定的在住院期间进行健康教育的路线图或表格。本科自2001年1月以来,运用健康教育路径     

多层螺旋CT三维重组及二维多平面重组技术在胃部疾病中的应用

随着计算机技术的发展,三维重组技术在胃部疾病中的应用逐渐增多。就多种胃部疾病在二维多平面重组及三维重组技术(包括仿真胃镜及透明技术)中的影像学表现以及该技术的应用价值及局限性予以综述。     

双氯芬酸钠缓释片致严重过敏反应1例

1病例资料 患者男,50岁,4月前因反复腰背部疼痛,在当地医院查骶髂关节CT,提示强直性脊柱炎,入院诊断为强制性脊柱炎,治疗具体用药不详,好转后出院。出院后,患者于2012年6月21日开始服用双氯芬酸钠缓释片（北京诺华制药有限公司,规格：75 mg/片,批号：X0377）75 mg, po, qd抗炎镇痛治疗,期间未服用其他药物。     

构建反义RNA表达载体对β-地中海贫血进行基因治疗研究

β地中海贫血(简称β地贫)是常见的、严重危害人民身体健康的遗传性血液病,在我国南方(两广地区)的发病率约为1％。该病目前尚无有效的根治方法,患者只有依赖定期输血才能维持其生命,但输血过度将引起铁质大量沉积,最终导致心力衰竭而过早地夭亡。因此,探索β地贫治疗途径是疾病防治工作的一项重要任务。     

1例Bowen's病复发患者外阴切除术的护理

对4例乳癌乳房切除术后患者应用腹壁下动脉穿支皮辩进行乳房再造术，结果移植皮辩成活良好，乳房柔软度好，形态满意。提出加强术前心理护理，术后密切监护移植皮辩，做好出院指导，是保证手术成功的主要措施。     

Rehabilitation therapy centralized on facilitation and acupuncture on upper extremities spasm after stroke

AIM:To observe the therapeutic effect of rehabilitation therspy centralized on facilitation and acupuncture on upper extrmilies spasm after stroke.METHODS:56 cases of stroke patients with upper extremities spasm were divided into two groups by random.28 patients in treatment group were received rehabilitation therapy centralized on facilitation and acupuncture treatment.Others in control group were received traditional method.RESULT:All patients were evaluated by muscle tone evalustion of Ashworth Scale.The outcomes of two groups has significant different(P&;lt;0.01).CONCLUSION:We could archive the satisfactory effects with acupuncture and rehabilitation therapy centralized on facilitation on upper extremities spasm of stroke patients.