腹腔镜与开腹手术治疗卵巢子宫内膜异位囊肿的对比研究

目的观察腹腔镜手术治疗卵巢子宫内膜异位囊肿的应用效果。方法将76例子宫内膜异位囊肿患者随机分为观察组和对照组组各38例。观察组行腹腔镜卵巢子宫内膜异位囊肿剥除术,对照组行常规开腹子宫内膜异位囊肿剥除术。观察2组术中出血量、手术时间、术后排气时间及住院时间。结果观察组术中出血量少于对照组,手术时间、术后排气时间及住院时间均短于对照组,差异均有统计学意义(P<0.05)。结论腹腔镜手术治疗卵巢子宫内膜异位囊肿效果显著,优于开腹手术治疗。     

前药热化疗对裸鼠转基因结肠癌肝转移的靶向作用

目的 探讨组织特异性胞嘧啶脱氨酶/5-氟胞嘧啶(CD/5-FC)系统热化疗对裸鼠结肠癌肝转移的靶向治疗效果.方法 脂质体法转CD基因至人结肠癌LoVo细胞株.45只BALB/c裸鼠采用经门静脉注射转CD基因LoVo细胞(LoVo-CEACD,0.2 mL,107/L)至裸鼠体内建立模型后,随机分为3组,每组15只,即对照组、前药治疗组及前药热疗组.3组分别经腹腔注射42℃生理盐水和38℃5-FC及42℃5-FC.5-FC剂量为500 mE/(kg·d),连续21 d后,待动物自然死亡,观察自然生存期、肝脏转移率、肝脏转移结节数;切取肝脏转移瘤组织,正常肝组织及胃、肺、胰腺、小肠、大肠组织,行RT-PCR检测各组织CD基因表达,观察各组织病理学变化、肿瘤细胞凋亡指数.结果 目的基因在肝脏转移瘤组织中能有效表达,在正常肝组织及胃、肺、胰腺、小肠、大肠组织中无表达.3组的自然生存期分别为(25.80±3.65),(34.27±4.08).(41.87±3.91)d;肝脏转移率分别为100.0%(15/15).40.0%(6/15),13.3%(2/15);平均肝脏转移结节数分别为(2.93±1.16),(0.80±1.01).(0.20±0.56)个.前药治疗组和前药热疗组的自然生存率明显长于对照组(P<0.05);3组间的肝脏转移率两两比较,差异均有统计学意义(均P<0.05).肿瘤细胞凋亡指数(AI)分别为4.59%,9.87%,17.4%,各组比较,差异均有统计学意义(P<0.05).结论 组织特异性CD/5-FC系统热化疗对裸鼠结肠癌肝转移有明显的靶向抑制作用.     

慢性胰腺炎并发门静脉高压症

目的 探讨慢性胰腺炎并发门静脉高压症的诊断和治疗.方法 回顾性分析该院1990年3月至2005年10月收治的慢性胰腺炎所致的胰源性门静脉高压症21例,其中左侧门静脉高压症16例,肝前型门静脉高压症5例.其诊断依据主要依靠多普勒超声、CT和MR等影像学检查和上消化道内镜检查,肝前性门静脉高压症有时须行MRA或肠系膜上动脉血管造影.治疗左侧门静脉高压症可行脾切除术,肝前型门静脉高压症有出血史者可行肠系膜上静脉、下腔静脉分流术,必要时加行脾切除术.结果 21例均获随访,随访时间1～5年,病人恢复良好,脾亢纠正,15例行上消化道内镜复查,曲张静脉消失或仅有轻度曲张,10例有出血史者未再发生出血.结论 注意慢性胰腺炎所致的胰源性左侧门静脉高压症的诊断治疗的同时应注意SMV-PV阻塞所致的肝前性门静脉高压症的诊治.     

全氟辛烷磺酸对大鼠仔鼠学习记忆能力的影响

Objective To study the effects of prenatal and postnatal perfluorooetane sulfonate (PFOS) exposure on spatial learning and memory, N-methyl-D-aspartate receptor 2B (NR2B) mRNA and protein level in frontal cortex and hippoeampus of rat pups and to explore the mechanism of developmental neurotoxicity induced by PFOS. Methods Twenty-eight pregnant mrs were randomly divided into three groups in proportion of 3: 2: 2,including control group (C), low dose group (L) and high dose group (H) by means of randomized number table, which respectively received 0,7.2, 14.4 mg/kg PFOS feed from pregnancy day 0 to postnatal day(PND)30 by free feedings. The animal models of prenatal and postnatal non-exposure (CC), prenatal exposure (LC and HC), postnatal exposure (CL and CH), and prenatal and postnatal exposure (LL and HH) to PFOS were established by cross-fostering method. The spatial learning and memory were measured by water maze experiment,the NR2B mRNA levels in frontal cortex of rat pups was determined with semi-quantitative RT-PCR, NR2B protein express in cerebral cortex (frontal and tempera]cortex) and hippocampus (CAI, CA3, CA4 and DG regions) of rat pups was detected by immunohistochemistry. Results The escape latency of CL, CH, LL and HH groups pups in water maze experiment were (99. 83±25.77) s, (111.30±17. 82) s, (106. 40±18. 71) s, (107.70±16. 85) s, and longer as compared with CC group[(54.90±26.69)s](q value were 4.349,4.773,6.026 and 5.641, respectively,P<0. 01). The number of errors of HH group rat pups entering dead end was (22. 30±7.56) at the training day 4,and it was significantly higher than that of CC group (9. 80±4. 64) (q=5. 173,P<0. 01). The NR2B mRNA levels of frontal cortex of pups in HC group at PND1 ,and LC group, HC group and HH group at PND14 were (0. 167±0. 008), (0. 364±0. 035), (0. 341±0. 030) and (0. 328±0. 045) respectively,which were significantly lower than CC group (0.271±0.060) and (0.465±0.067) (q values were 3. 547, 3. 739, 4. 597 and 5. 006, respectively, P<0.05) . The results of immunohistochemistry indicated that NR2B protein express of the hippocampus CA1 region of pups in LC group was (0.091±0.005),and showed significant lower than CC group which was (0. 123±0.009) at PND1 (q=5. 209 ,P<0. 05). At PND14, the effect of PFOS extended to cerebral cortex and hippocampus regions. At PND28, the effects of PFOS were showed in hippocampus CA1, CA3 and temporal cortex regions. Conclusion Prenatal and postnatal exposure to PFOS should result in the spatial learning and memory damage,and the mechanism might be possibly involved in the decrease of NR2B level in cerebra lcortex and hippocampal formation regions.     

S100钙结合蛋白A4在大鼠动脉粥样硬化中的作用

目的探索钙离子拮抗剂(CCB)和干预S100钙结合蛋白A4(S100A4)表达对SD大鼠下肢动脉硬化性闭塞症(ASO)动物模型发病进程的影响。方法将56只健康SD大鼠采用随机数字表分组法等分为8组, 每组7只。A组为对照组, B组为假手术组, C、D、E、F、G、H组则为通过采用手术联合高脂饮食的方式构建大鼠经典ASO模型后, 鼠尾静脉注射CCB、Lv-S100A4、S100A4小干扰RNA(siRNA)。通过茜素红染色评估股动脉中Ca2+浓度的改变, 苏木精-伊红(HE)染色评估股动脉内膜、中膜增生情况, 免疫组织化学检测股动脉S100A4的蛋白表达。计量资料两组间比较用独立样本t检验;多组间比较用单因素方差分析。计数资料采用χ2检验。等级资料采用秩和检验。结果 16周后成功构建大鼠ASO模型。F组中S100A4的表达显著低于E组(0.24±0.03比0.42±0.05, t=-6.774, P<0.01)且F组股动脉内膜及中膜厚度明显低于E组[(82.98±4.76) μm比(101.26±3.18) μm, t=-8.451, P<0.01]。H组中S100A4的表达...     

国医大师王烈教授从肾脑论治儿童抽动障碍经验

抽动障碍是一种以不随意的、突然发生的、快速的、反复出现的、无明显目的的、非节律性的一个或多个部位运动抽动和（或）发声抽动为主要特征的综合征，王烈教授认为抽动障碍的病机关键在于脑、肾不足，发病与五脏六腑功能失调相关，治疗上应注重安脑滋肾，治病求本，同时强调家庭精神调护，临床上取得良好的疗效。