钙黏素在某些皮肤病发病机制中的意义

钙黏素是细胞与细胞间黏附的主要成分,对皮肤组织的生长、分化及维持其完整性有重要作用。目前的研究表明钙黏素在一些皮肤病中的表达及活性均有异常。综述3种钙黏素在一些皮肤病中表达特征及在发病机制的作用,为皮肤病的诊断和治疗提出了新思路。     

Construction of Prokaryotic Expression Plasmid of mtrC Protein of Neisseria gonorrhoeae and Its Expression in E.Coli

Gonorrheais one of the most common venerealdiseases.The wild use of antibiotic againstNeisse-ria gonorrhoeaehas made the drug resistance a bigproblemin the prevention and treat ment of gonor-rhea.Besides the drug resistance mediated bychange in structure of penicillin-binding protein,mutation of DNAgyrase A,chromosome plasmid,another non-specificity multi-drug resistant mecha-nismofNeisseria gonorrhoeae,multiple transfera-ble resistance efflux system,has been paid closeattention to increasin…     

核转录因子NF-κB和血管内皮生长因子在不同病程尖锐湿疣中的表达及其意义

目的:探讨核转录因子NF-κB和血管内皮生长因子(vascular endothelial growth factor,VEGF)在不同病程尖锐湿疣(CA)中的表达情况及其临床意义.方法:将确诊的尖锐湿疣患者40例分为短病程组20例、长病程组20例,同时以20例正常人包皮组织为对照组,用RT-PCR和Western Blot的方法分别检测不同病程CA以及正常皮肤组织中的核转录因子NF-κB和VEGF的mRNA及蛋白表达.结果:(1)短病程组CA疣体中NBκB mRNA、YEGFmRNA的表达水平分别为1.16±0.17、1.01±0.11,长病程组中NF-κB mRNA、VEGF mRNA的表达水平分别为0.75±0.10、1.11±0.10,均明显高于对照组的0.47±0.11和0.61±0.12(均P＜0.05);同时短病程组CA疣体中NF-κB mRNA、VEGF mRNA的表达水平与长病程组比较差异有显著性(P＜0.05).(2)短病程组CA疣体中NF-κB蛋白、VEGF蛋白表达水平分别为0.78±0.12、0.81±0.12,长病程组中NF-κB蛋白、VEGF蛋白表达水平分别为0.66±0.11、0.91±0.11,显著高于对照组的0.31±0.08和0.29±0.09(均P＜0.05).NF-κB蛋白以短病程组表达最高,且短病程组与长病程组比较差异有显著性(P＜0.05).结论:核转录因子NF-κB和血管内皮生长因子在尖锐湿疣疣体中高表达并与病程相关,可能共同参与了CA的发病以及转归.     

孕酮在淋球菌引起的中性粒细胞炎症反应中的调节作用

目的 研究孕酮在淋球菌引起的中性粒细胞炎症反应中的作用。方法 提取正常人外周血中性粒细胞,根据是否加入孕酮,将其分为孕酮组、淋球菌组、干预组（孕酮 + 淋球菌）及对照组。荧光定量RT-PCR分别测定在0、3、8、12 h各组中性粒细胞中诱导型一氧化氮合酶（iNOS）、TNF-α、IL-1β mRNA含量,并用Western印迹测定iNOS蛋白水平。结果 淋球菌组和干预组中iNOS、TNF-α、IL-1β mRNA表达水平均升高;淋球菌组在8 h达到高峰,以后逐渐下降;干预组三者水平明显低于淋球菌组（P < 0.05）。而孕酮组、对照组各因子含量无明显变化。Western印迹结果显示,淋球菌组和干预组iNOS蛋白表达水平亦升高,前者明显高于后者（P < 0.05）。结论 孕酮下调中性粒细胞iNOS、TNF-α及IL-1β的表达,抑制淋球菌引起的中性粒细胞炎症反应,这一机制可能在女性无症状感染中起作用。     

水疱型毛母质瘤1例

患者女,16岁.右上臂单个皮下结节2个月.表面出现水疱样外观1个月,起初局部有压痛感,现无不适,至就诊时"水疱"一直未破.发病前局部无外伤史.既往体健,患者家族中无类似疾病患者.     

NB-UVB辐射对HUVEC分泌sE-selectin及sICAM-1的影响

目的研究NB-UVB辐射对TNF-α刺激人脐静脉血管内皮细胞(HUVEC)表达可溶性E选择素(sE-se-lectin)及可溶性细胞间黏附分子(sICAM-1)的影响。方法体外培养人脐静脉血管内皮细胞(HU-VEC),将HUVEC接种于96孔塑料培养板,分为对照组,TNF-α组,TNF-α+NB-UVB60mJ/cm2剂量组,TNF-α+NB-UVB40mJ/cm2剂量组,TNF-α+NB-UVB20mJ/cm2剂量组。TNF-α刺激浓度为10ng/mL;NB-UVB高,中,低剂量组剂量分别为20,40,60mJ/cm2。采集上清,ELISA法检测可溶性E选择素(sE-selectin)和可溶性细胞间黏附分子1(sICAM-1)含量。结果 NB-UVB能剂量依赖性地抑制TNF-α刺激后HUVEC分泌sE-selectin和sICAM-1。结论 NB-UVB可能通过抑制血管内皮细胞表达黏附分子在治疗炎症性皮肤病中发挥免疫调节作用。     

银屑病患者抑郁情绪与皮损中SP和IL-23水平的相关研究

目的:确定银屑病患者抑郁情绪的发生率及与皮损中P物质(SP),白介素-23(IL-23)水平的相关性.方法:采用抑郁他评量表中HAMD及抑郁自评量表中SDS两种量表评定银屑病患者心理状况;分离培养银屑病患者皮损和正常人表皮中角质形成细胞,ELISA方法检测SP,IL-23水平;同时将心理因素与SP和IL-23水平进行Pearson 相关分析.结果:在HAMD 量表中,实验组抑郁发生率为59.26%(32/54),重度抑郁为16.67%(9/54);SDS量表中,实验组的抑郁发生率为53.70%(29/54),重度抑郁为18.52%(10/54).实验组患者的SP和IL-23水平显著高于对照组(P<0.05).HAMD量表各因子及SDS得分与SP和IL-23水平均呈显著正相关.结论:抑郁可能通过影响某些神经免疫通道参与银屑病发病.     

米诺地尔外用对化疗后小鼠ICAM-1和ELAM-1的影响

目的: 评价米诺地尔对小鼠血清和毛囊ICAM-1和ELAM-1水平的影响.方法: 环磷酰胺静脉注射建立C57BL/6小鼠化疗后脱发模型.米诺地尔酊涂抹小鼠背部皮肤后,切取皮肤标本观察毛囊组织学变化;应用ELISA法和免疫组化法检测小鼠血清及毛囊内ICAM-1和ELAM-1水平.结果: 小鼠于注射环磷酰胺后4天出现明显的弥漫性脱毛,组织学上毛囊多为退行期,米诺地尔组多为生长期毛囊.造模后小鼠血清ICAM-1和ELAM-1的水平分别为(46.57±10.25)ng/mL和(28.57±6.03)ng/mL,高于对照组(P<0.05);米诺地尔涂抹后血清ICAM-1和ELAM-1水平分别为(27.21±5.62)ng/mL和(17.21±4.80)ng/mL,明显低于模型组(P<0.05).结论: 米诺地尔可减缓环磷酰胺导致的生长期毛囊细胞凋亡和退行性变,并降低血清和毛囊局部ICAM-1和ELAM-1水平,对环磷酰胺所致的脱发有一定的防治作用.     

Association of rs10954213 polymorphisms and haplotype diversity in interferon regulatory factor 5 with systemic lupus erythematosus: A meta-analysis

The rs10954213 polymorphism and the haplotype diversity in interferon regulatory factor 5 (IRF5) play a special role in systemic lupus erythematosus (SLE) but with inconclusive results. We conducted a meta-analysis integrating case-control and haplotype variant studies in multiple ethnic populations to clearly discern the effect of these two variants on SLE. Eleven studies on the relation between rs10954213 polymorpisms in IRF5 and SLE were included and we selected a random effect model to calculate the pooled odds ratios (ORs) and the corresponding 95% confidence interval (95% CI). A total of 6982 cases and 8077 controls were involved in the meta-analysis. The pooled results indicated that A allele was significantly associated with increased risk of SLE as compared with the IRF5 rs10954213 G allele (A vs. G, P<0.00001) in all subjects. The same pattern of the results was also obtained in the European, African American, and Latin American. Asian population had a much lower prevalence of the A allele (49.1%) than any other population studied, and Europeans had the highest frequency of the IRF5 rs10954213 A allele (62.1%). The significant association of increased SLE risk and TCA haplotype was indicated in the contrast of TCA vs. TTA as the pooled OR was 2.14 (P=0.002). The same result was also found in the contrast of TCA vs. TTG as the pooled OR was 1.45 (P=0.004). This meta-analysis suggests that the A allele of rs10954213 and TCA haplotype (rs2004640-rs2070197-rs10954213) in IRF5 is associated with the increased risk of SLE in different ethnic groups, and its prevalence is ethnicity dependent.     

树突状细胞分型进展与抗白念珠菌特异性免疫

树突状细胞(DC)因其特有的捕捉、吞噬、提呈抗原的能力在其被发现的初期就引起人们极大的研究热情。近年来随着DC培养、分离技术的突破,对DC的认识也日益深入。目前认为DC亚群的多样性及其功能的可塑性是其在免疫应答和免疫调节中多样化作用的重要基础。