高糖危象患者血清瘦素和氧化应激的关系

Objective To investigate the relationship between the levels of serum leptin and oxidative stress in patients with hyperglycemia crisis. Methods A total of 96 patients with diabetic ketoaeidosis (DKA) and nonketotic hyperglycemia (NKH) were treated on a low-dose insulin protocol using intravenous infusion of insulin with the established rate of 0.1U·kg-1·h-1,with the patients on intravenous fluids and receiving nutrition by mouth and vein. The levels of serum leptin, 8-iso-prostaglandin F2α (8-iso-PGF2α, the activities of superoxide dismutase (SOD), total antioxidant capacity (TAC) and the contents of malondialdehyde (MDA) in 96 patients with hyperglycemia crisis on admission and after insulin therapy with resolution of hyperglycemia and ketoacidosis (72 hours) were measured. Another 35 healthy individuals served as normal control. Results The activities of SOD, TAC and the levels of leptin before treatment were lower in patients with hyperglycemia crisis than in normal controls, and the levels of MDA and 8-iso-PGF2a were more markedly elevated than those in normal controls (all P<0. 05). The activities of SOD, TAC and the levels of leptin in patients after treatment were significantly higher than those in patients before treatment, and the levels of MDA and 8-iso-PGF2a. were significantly lower than those in patients on admission (all P<0. 05). There was significant positive correlation between leptin and MDA in patients before treatment (r=0. 38, P<0. 05), and the level of leptin was negatively correlated with MDA and 8-iso-PGF2a in patients after treatment (r1 = - 0. 35, r2= - 0. 37, both P < 0. 05). In stepwise regression analysis, MDA and 8-iso-PGF2α showed a significant association with leptin. Conclusion The levels of leptin are significantly lowered in patients with hyperglycemia crisis. Oxidative stress may participate in determining the leptin level in hyperglycemia crisis.     

高糖危象患者血清瘦素和氧化应激的关系

Objective To investigate the relationship between the levels of serum leptin and oxidative stress in patients with hyperglycemia crisis. Methods A total of 96 patients with diabetic ketoaeidosis (DKA) and nonketotic hyperglycemia (NKH) were treated on a low-dose insulin protocol using intravenous infusion of insulin with the established rate of 0.1U·kg-1·h-1,with the patients on intravenous fluids and receiving nutrition by mouth and vein. The levels of serum leptin, 8-iso-prostaglandin F2α (8-iso-PGF2α, the activities of superoxide dismutase (SOD), total antioxidant capacity (TAC) and the contents of malondialdehyde (MDA) in 96 patients with hyperglycemia crisis on admission and after insulin therapy with resolution of hyperglycemia and ketoacidosis (72 hours) were measured. Another 35 healthy individuals served as normal control. Results The activities of SOD, TAC and the levels of leptin before treatment were lower in patients with hyperglycemia crisis than in normal controls, and the levels of MDA and 8-iso-PGF2a were more markedly elevated than those in normal controls (all P<0. 05). The activities of SOD, TAC and the levels of leptin in patients after treatment were significantly higher than those in patients before treatment, and the levels of MDA and 8-iso-PGF2a. were significantly lower than those in patients on admission (all P<0. 05). There was significant positive correlation between leptin and MDA in patients before treatment (r=0. 38, P<0. 05), and the level of leptin was negatively correlated with MDA and 8-iso-PGF2a in patients after treatment (r1 = - 0. 35, r2= - 0. 37, both P < 0. 05). In stepwise regression analysis, MDA and 8-iso-PGF2α showed a significant association with leptin. Conclusion The levels of leptin are significantly lowered in patients with hyperglycemia crisis. Oxidative stress may participate in determining the leptin level in hyperglycemia crisis.     

高糖危象患者血清瘦素和氧化应激的关系

目的 探讨高糖危象患者血清瘦素水平变化及与氧化应激的关系.方法 选择糖尿病酮症酸中毒和非酮症高血糖患者96例,静脉泵入胰岛素0.1 U·kg1·h1,同时给予补液、对症治疗以及胃肠和静脉营养.检测高糖危象患者治疗前及病情缓解时(72 h)血清瘦素、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、总抗氧化能力(TAC)和8-异前列腺素F2α(8-iso-PGF2α)水平.另外选择35例健康体检者作为对照组.结果 高糖危象患者治疗前SOD、TAC和瘦素水平均显著低于健康对照组,而MDA和8-iso-PGF2a均显著高于健康对照组(P均<0.05);高糖危象患者治疗后SOD、TAC和瘦素均显著高于治疗前,而MDA和8-iso-PGF2a均显著低于治疗前(P均<0.05).高糖危象患者治疗前瘦素与MDA呈显著正相关(r=0.38,P<0.05),治疗后瘦素与MDA和8-iso-PGF2a呈显著负相关(r1=-0.35,r2=-0.37,P均<0.05).多元逐步回归分析显示MDA和8-iso-PGF2α均为影响血清瘦素水平的显著因素.结论 高糖危象患者血清瘦素水平明显下降,且与氧化应激有关.     

糖尿病大鼠肋间肌微量元素与氧化应激变化的研究

目的：了解糖尿病肋间肌微量元素与氧化应激的关系。方法:采用原子吸收光谱法,测定糖尿病28 d大鼠肋间肌组织中铜(Cu)、锰(Mn)、锌(Zn)、铁(Fe)、镉(Cd)、铬(Cr)、钴(Co)、钼(Mo)和锂(Li)的含量,并同时测定超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:糖尿病肋间肌Cu、Zn、Fe、Cr、Li含量明显低于对照组, Cd明显高于对照组,而Mn、Co 、Mo 与对照组无明显差别。糖尿病肋间肌组织SOD活性明显低于对照组，MDA含量明显高于对照组。糖尿病肋间肌组织Zn和Cu含量与其SOD活性呈正相关, Cd含量与SOD 活性呈负相关，Zn与MDA含量呈负相关。结论:糖尿病28 d 肋间肌组织存在Cu、Zn、Fe、Cd、Cr、Li 含量的变化及氧化应激增加,Zn、Cu和Cd 在糖尿病肋间肌组织氧化应激变化中具有重要意义。     

糖尿病大鼠膈肌酶组织化学研究

目的:观察糖尿病早期膈肌酶组织化学变化,以了解糖尿病膈肌的损害。方法:应用酶组织化学方法观察糖尿病4周大鼠膈肌组织脱氢酶、水解酶和氧化酶活性变化。结果:糖尿病4周大鼠膈肌组织琥珀酸脱氢酶、苹果酸脱氢酶、异柠檬酸脱氢酶、谷氨酸脱氢酶、辅酶Ⅰ黄递酶、葡萄糖-6-磷酸脱氢酶、酸性磷酸酶和酸性-α-萘酸性酯酶活性强于对照组,乳酸脱氢酶和细胞色素氧化酶活性弱于对照组,辅酶Ⅱ黄递酶无明显改变,5'-核苷酸酶不着色。图像分析显示糖尿病大鼠膈肌组织琥珀酸脱氢酶、苹果酸脱氢酶、异柠檬酸脱氢酶、谷氨酸脱氢酶、辅酶Ⅰ黄递酶、葡萄糖-6-磷酸脱氢酶、酸性磷酸酶和酸性-α-萘酸性酯酶着色深度(A)明显高于对照组,而乳酸脱氢酶和细胞色素氧化酶明显低于对照组,辅酶Ⅱ黄递酶无明显差异。结论:糖尿病4周大鼠膈肌组织有氧氧化能力增强、糖酵解能力减弱、脂肪及能量代谢紊乱。     

高糖危象患者血清瘦素和氧化应激的关系

Objective To investigate the relationship between the levels of serum leptin and oxidative stress in patients with hyperglycemia crisis. Methods A total of 96 patients with diabetic ketoaeidosis (DKA) and nonketotic hyperglycemia (NKH) were treated on a low-dose insulin protocol using intravenous infusion of insulin with the established rate of 0.1U·kg-1·h-1,with the patients on intravenous fluids and receiving nutrition by mouth and vein. The levels of serum leptin, 8-iso-prostaglandin F2α (8-iso-PGF2α, the activities of superoxide dismutase (SOD), total antioxidant capacity (TAC) and the contents of malondialdehyde (MDA) in 96 patients with hyperglycemia crisis on admission and after insulin therapy with resolution of hyperglycemia and ketoacidosis (72 hours) were measured. Another 35 healthy individuals served as normal control. Results The activities of SOD, TAC and the levels of leptin before treatment were lower in patients with hyperglycemia crisis than in normal controls, and the levels of MDA and 8-iso-PGF2a were more markedly elevated than those in normal controls (all P<0. 05). The activities of SOD, TAC and the levels of leptin in patients after treatment were significantly higher than those in patients before treatment, and the levels of MDA and 8-iso-PGF2a. were significantly lower than those in patients on admission (all P<0. 05). There was significant positive correlation between leptin and MDA in patients before treatment (r=0. 38, P<0. 05), and the level of leptin was negatively correlated with MDA and 8-iso-PGF2a in patients after treatment (r1 = - 0. 35, r2= - 0. 37, both P < 0. 05). In stepwise regression analysis, MDA and 8-iso-PGF2α showed a significant association with leptin. Conclusion The levels of leptin are significantly lowered in patients with hyperglycemia crisis. Oxidative stress may participate in determining the leptin level in hyperglycemia crisis.     

肥胖男性患者血浆8异前列腺素F2α与细胞黏附分子的关系

目的 探讨男性单纯肥胖患者血浆8异前列腺素F2α水平与细胞黏附分子的关系.方法 测定36例男性肥胖患者和30例健康非肥胖男性个体的血8异前列腺素F2α(8～iso-PGF2α),可溶性细胞间黏附分子1(sICAM-1)和E选择素.结果 肥胖患者体质量指数(BMI)、腰围/臀围(WHR)、空腹胰岛素、胰岛素抵抗指数(HOMA-IR)、血浆8-iso-PGF2α、sICAM-1和E选择素均显著高于正常个体(P＜0.05或P＜0.01).男性肥胖组相关分析:8-iso-PGF2α与BMI、E选择素和HOMA-IR呈正相关(相关系数分别为0.28,0.33,0.31,P＜0.05).多元逐步回归分析:校正年龄和BMI后,偏相关分析显示,血浆8-iso-PGF2α仍与E选择素呈正相关(r=0.265,P＜0.05).结论 血浆8-iso-PGF2α可能是男性单纯肥胖患者发生细胞黏附分子变化的重要因素之一.     

2型糖尿病高血压患者血浆内源性分泌型晚期糖基化终末产物受体和氧化应激的关系

探讨2型糖尿病高血压患者血浆内源性分泌型晚期糖基化终末产物受体(esRAGE)与氧化应激的关系.结果 显示2型糖尿病患者esRAGE与体重指数(BMI,r=-0.33)、舒张压(r=-0.38)、氧化应激均显著相关(均P相似文献