2004～2005年广西HIV-1耐药横断面调查

目的 了解广西壮族自治区未接受抗病毒治疗的HIV感染者和已经接受抗病毒治疗一段时间的AIDS病人中的耐药情况.方法 收集我区2004～2005年部分HIV感染者的伞血标本201份,其中未接受抗病毒治疗的56人(27.86%),已接受抗病毒治疗的145人(72.14%),分别对他们进行问卷调查,并采集10毫升的抗凝血,分...     

广西地区未感染HIV婴儿T淋巴细胞及亚群的研究

目的探讨广西地区未感染艾滋病病毒（HIV）婴儿T淋巴细胞及亚群的基本特征,为初步排除并辅助诊断HIV阳性产妇所生婴儿是否感染HIV提供依据。方法收集68例未感染HIV婴儿（未感染组）和13例感染HIV婴儿（对照组）的抗凝全血,用四色荧光流式细胞术检测外周血CD3＋、CD4＋、CD8＋T淋巴细胞绝对数及其百分比,并计算CD4/CD8比值;用Nuclisens EasyQ方法检测血浆HIV-1病毒载量。结果 （1）未感染组外周血CD3＋、CD4＋、CD8＋T淋巴细胞绝对数（细胞/mm3）的中位数分别为4 5702、8181、410;CD3、CD4、CD8的百分比中位数分别为61.42%、39.39%、18.40%;CD4/CD8比值的中位数为2.25;在未感染组中,男性婴儿与女性婴儿的T淋巴细胞及亚群的比较,各检测指标差异没有统计学意义（P〉0.05）;血浆HIV-1病毒载量均为未检测出水平。（2）对照组外周血CD3＋、CD4＋、CD8＋T淋巴细胞绝对数的中位数分别为4 930、1 4603、326（细胞/mm3）;CD3、CD4、CD8百分比的中位数分别为69.95%、13.77%、54.69%;CD4/CD8比值的中位数为0.25;血浆HIV-1病毒载量的中位数为800 000（拷贝/ml）。（3）除CD3＋T淋巴细胞绝对数之外,未感染组与对照组的CD4＋、CD8＋T淋巴细胞绝对数、CD3、CD4、CD8百分比及CD4/CD8比值的比较,差异有统计学意义（P〈0.05）。结论在资源有限的情况下,参考婴儿T淋巴细胞及亚群的异常变化,可初步判断HIV阳性产妇所生婴儿是否感染HIV,T淋巴细胞及亚群的检测,可以作为一种辅助诊断HIV感染的手段。     

中国南方四省区流行的HIV-1 CRF01_AE病毒株基因特征研究

Objective To analyze the genetic characteristics of HIV-1 CRF01_AE strains prevailing in. the four provinces of southern China. Methods Plasma samples were collected from the newly diagnosed HIV-1 individuals reported in 2006 in Guangdong, Guangxi, Jiangxi and Hunan province. The gag and env gene fragments were amplified from RNA template extracted from plasma using RT and nested PCR methods. CRF01_AE sequences were analyzed by phylogcnetie methods and characterized by calculating the genetic distance and Entropy analysis. Results Two main epidemic clusters were found to exist in the CRF01 AE strains from 210 HIV-1 CRF01 AE infected individuals collected in the 4 provinces, southern China. It was found that no international reference strain was closely correlated with cluster Ⅰ , which including 123 samples. The strains in cluster Ⅱ, consisting 57 cases of samples, were closely related with the strains identified in Vietnam. Genetic distance analysis of gag and env genes showed that the diversity of cluster Ⅰ was obviously less than that of cluster Ⅱ. Data on nucleotide polymorphism showed that nucleotides compositions of 42 sites in gag and 40 sites in env wer esignificantly different between the two clusters. When compared with cluster Ⅱ , the polymorphism decreased at 61 nucleotide sites but increased at 21 sites in cluster Ⅰ. Conclusion This was the first report describing that two main epidemic clusters were existed in CRF01_AE strains prevailing in the 4 provinces, Southern China. The vires in cluster Ⅰ was the dominant strain in this region, with shorter period of circulation and higher proportion seen in the HIV-infected population, which might belong to CRF01_AE strain with certain features facilitating the spread of the virus. The virus in cluster Ⅱ was highly homology with the CRF01_AE strains from Vietnam, and seemed to have had several events of epidemics in populations in border regions of China and Vietnam.     

中国南方四省区流行的HIV-1 CRF01_AE病毒株基因特征研究

Objective To analyze the genetic characteristics of HIV-1 CRF01_AE strains prevailing in. the four provinces of southern China. Methods Plasma samples were collected from the newly diagnosed HIV-1 individuals reported in 2006 in Guangdong, Guangxi, Jiangxi and Hunan province. The gag and env gene fragments were amplified from RNA template extracted from plasma using RT and nested PCR methods. CRF01_AE sequences were analyzed by phylogcnetie methods and characterized by calculating the genetic distance and Entropy analysis. Results Two main epidemic clusters were found to exist in the CRF01 AE strains from 210 HIV-1 CRF01 AE infected individuals collected in the 4 provinces, southern China. It was found that no international reference strain was closely correlated with cluster Ⅰ , which including 123 samples. The strains in cluster Ⅱ, consisting 57 cases of samples, were closely related with the strains identified in Vietnam. Genetic distance analysis of gag and env genes showed that the diversity of cluster Ⅰ was obviously less than that of cluster Ⅱ. Data on nucleotide polymorphism showed that nucleotides compositions of 42 sites in gag and 40 sites in env wer esignificantly different between the two clusters. When compared with cluster Ⅱ , the polymorphism decreased at 61 nucleotide sites but increased at 21 sites in cluster Ⅰ. Conclusion This was the first report describing that two main epidemic clusters were existed in CRF01_AE strains prevailing in the 4 provinces, Southern China. The vires in cluster Ⅰ was the dominant strain in this region, with shorter period of circulation and higher proportion seen in the HIV-infected population, which might belong to CRF01_AE strain with certain features facilitating the spread of the virus. The virus in cluster Ⅱ was highly homology with the CRF01_AE strains from Vietnam, and seemed to have had several events of epidemics in populations in border regions of China and Vietnam.     

基于分子网络的广西壮族自治区HIV传播热点和跨地区传播特征分析

目的 基于分子网络分析广西壮族自治区（广西）HIV传播热点和跨地区传播特征,为优化艾滋病精准防控策略提供证据。方法 整合1997-2020年采集的5 996条广西HIV pol区序列和165 534条公开发表的非广西HIV pol区序列,使用HIV-TRACE工具以0.5%成对基因距离阈值构建分子网络。结果 进入广西HIV传播热点分子网络的序列比例为31.5%（1 886/5 996）。在HIV跨地区传播分子网络中,省内连接占51.6%（2 613/5 062）,国内连接占48.0%（2 430/5 062）,国际连接占0.4%（19/5 062）。与广西跨地区连接的主要地区为广东省（49.5%,1 212/2 449）、北京市（17.5%,430/2 449）、上海市（6.9%,168/2 449）、四川省（5.7%,140/2 449）、云南省（4.2%,102/2 449）、陕西省（3.8%,93/2 449）、浙江省（2.8%,69/2 449）、海南省（2.0%,49/2 449）、安徽省（1.5%,37/2 449）和江苏省（1.3%,33/2 449）,其余地区与广西跨地区连接比例均<1.0%。进入广西HIV传播热点分子网络风险较高的影响因素包括≥50岁（相比于25~49岁,aOR=1.68,95%CI：1.46~1.95）、男性（相比于女性,aOR=1.21,95%CI：1.05~1.40）、未婚（相比于已婚,aOR=1.18,95%CI：1.00~1.39）、高中及以上文化程度（相比于初中及以下,aOR=1.21,95%CI：1.04~1.42）和男男性传播（相比于异性性传播,aOR=1.77,95%CI：1.48~2.12）。跨地区连接风险较高的影响因素包括男性（相比于女性：aOR=1.74,95%CI：1.13~2.75）,高中及以上文化程度（相比于初中及以下,aOR=1.96,95%CI：1.43~2.69）、自由职业/待业/退休（相比于农民,aOR=1.50,95%CI：1.07~2.11）、男男性传播（相比于异性性传播,aOR=3.28,95%CI：2.30~4.72）。结论 广西存在HIV传播热点,广西与国内地区组成复杂的跨地区传播网络。后续研究应针对分子网络分析推断的高风险人群开展社会网络调查,及时识别隐匿传播链,减少HIV二代传播。     

广西壮族自治区2020年新报告≥50岁男性HIV感染者的HIV-1基因亚型分布及其与首次CD4+T淋巴细胞的关联

  目的  分析2020年广西壮族自治区(简称广西)新报告50岁及以上男性(简称中老年男性)HIV感染者的HIV-1基因亚型构成特点及不同基因亚型与首次CD4+T淋巴细胞(简称CD4)计数的关联。  方法  以市为抽样单位,采用分层随机抽样法选取广西2020年新报告中老年男性HIV感染者为研究对象,对其血浆进行病毒RNA提取、逆转录扩增、测序、HIV-1基因亚型和亚簇判定,使用多因素模型评价CD4计数可能存在统计学关联的影响因素。  结果  共纳入分析606例HIV感染者,主要流行毒株有CRF01_AE、CRF07_BC及CRF08_BC三种,其构成比分别为57.6%(349/606)、17.2%(104/606)及22.1%(134/606),其首次CD4中位数分别为145(51, 271) 个/μL、212(110, 330) 个/μL及178(100, 307)个/μL。CRF01_AE感染者首次CD4值低于CRF07_BC感染者(Z=-3.363, P=0.002),也低于CRF08_BC感染者(Z=-2.983, P=0.009)。二元logistic回归分析模型分析显示,CRF01_AE为广西中老年人群CD4≤200个/μL的独立危险因素(aOR=1.453, 95% CI: 1.047~2.017)。  结论  CRF01_AE、CRF07_BC和CRF08_BC为广西2020年新报告中老年男性HIV感染者主要毒株,其构成以CRF01_AE为主,CRF01_AE为CD4≤200个/μL的独立危险因素,提示感染CRF01_AE毒株中老年男性的疾病进展较快,对广西艾滋病流行危害较大。     

一种国产HIV-1病毒载量试剂与进口试剂的比较研究

正血浆病毒载量检测是艾滋病防治工作中一项重要的实验室检测指标,与疾病的发生、发展及预后有密切关系,主要用于评估艾滋病病毒(HIV)感染进程、抗病毒治疗效果监测及HIV感染的辅助诊断~([1])。目前国内主要使用3种进口试剂进行病毒载量(VL)检测,进口试剂的敏感性及特异性均较好,但价格昂贵~([2-4])。随着治疗就是预防艾滋病策略被广泛地接受~([5-6]),我国免费抗艾滋病病毒治疗的病人数大幅度     

中国南方四省区流行的HIV-1 CRF01_AE病毒株基因特征研究

Objective To analyze the genetic characteristics of HIV-1 CRF01_AE strains prevailing in. the four provinces of southern China. Methods Plasma samples were collected from the newly diagnosed HIV-1 individuals reported in 2006 in Guangdong, Guangxi, Jiangxi and Hunan province. The gag and env gene fragments were amplified from RNA template extracted from plasma using RT and nested PCR methods. CRF01_AE sequences were analyzed by phylogcnetie methods and characterized by calculating the genetic distance and Entropy analysis. Results Two main epidemic clusters were found to exist in the CRF01 AE strains from 210 HIV-1 CRF01 AE infected individuals collected in the 4 provinces, southern China. It was found that no international reference strain was closely correlated with cluster Ⅰ , which including 123 samples. The strains in cluster Ⅱ, consisting 57 cases of samples, were closely related with the strains identified in Vietnam. Genetic distance analysis of gag and env genes showed that the diversity of cluster Ⅰ was obviously less than that of cluster Ⅱ. Data on nucleotide polymorphism showed that nucleotides compositions of 42 sites in gag and 40 sites in env wer esignificantly different between the two clusters. When compared with cluster Ⅱ , the polymorphism decreased at 61 nucleotide sites but increased at 21 sites in cluster Ⅰ. Conclusion This was the first report describing that two main epidemic clusters were existed in CRF01_AE strains prevailing in the 4 provinces, Southern China. The vires in cluster Ⅰ was the dominant strain in this region, with shorter period of circulation and higher proportion seen in the HIV-infected population, which might belong to CRF01_AE strain with certain features facilitating the spread of the virus. The virus in cluster Ⅱ was highly homology with the CRF01_AE strains from Vietnam, and seemed to have had several events of epidemics in populations in border regions of China and Vietnam.     

中国三省份未治疗HIV-1感染者临床毒株分离及其独特重组型的特征

目的分析未治疗的经性传播和注射吸毒感染HIV-1患者的病毒分离特征和独特重组型特征, 为了解不同传播途径感染HIV-1的病毒生物学特性和精准防控提供依据。方法根据不同的HIV-1传播风险, 筛选北京、广西、四川3个省市新诊断未治疗的HIV-1患者, 静脉采血检测病毒载量、CD4+ T细胞计数, 以及分离外周血淋巴细胞进行病毒分离, 从病毒培养上清中提取RNA扩增全长序列, 对序列进行分析。结果 65名HIV-1感染者中, 经男男性行为、异性性行为和注射吸毒感染分别为32(49.2%)、20(30.8%)和13(20.0%)例;亚型主要包括:26例(40.0%)CRF07_BC、23例(35.4%)CRF01_AE、9例(13.8%)独特重组型。共分离到46株HIV-1临床毒株, HIV-1分离阳性率与CD4+T细胞显著负相关(χ2=4.22, P=0.04), 而与病毒载量正相关(χ2=22.4, P<0.001);HIV-1的P24抗原含量多因素广义估计方程模型分析呈现类似结果。此外, 广义估计方程模型显示P24抗原含量与培养时间正相关(...     

2011年广西部分地区HIV耐药株发生情况分析

目的了解广西部分地区已接受高效抗反转录病毒治疗(HAART)的病人中,艾滋病病毒(HIV)耐药株的发生情况。方法对南宁市和崇左市2011年已接受HAART6个月的病人,进行横断面调查并采集血液样本检测HIV-1病毒载量,对病毒载量1000拷贝/mL的样本进行耐药基因型分析。结果两市共调查并成功采集了1472例病人的血液样本,病毒未抑制率为4.6%(68/1472),最终获得64份序列用于耐药分析。发现对非核苷类反转录酶抑制剂(NNRTIs)和核苷类反转录酶抑制剂(NRTIs)最主要耐药突变位点分别为G190A和M184V,未发现蛋白酶类抑制剂(PIs)有耐药突变,3类药物的耐药率分别为1.9%(28/1468)、1.6%(24/1468)和0.0%(0/1468),总耐药率为3.5%(52/1468)。52例病人出现了耐药,其中43.8%(28/64)病毒抑制失败病人对NNRTIs耐药,37.5%(24/64)对NRTIs耐药,29.7%(19/64)对NNRTIs和NRTIs双重耐药,81.3%(52/64)至少对某类药物有耐药。结论两个地区2011年已接受HAART人群中,HIV耐药株发生率较低,但出现了对NNRTIs和NRTIs双重耐药,值得关注。