慢性心力衰竭患者心型脂肪酸结合蛋白与超敏C-反应蛋白的变化及其相关性

Objective To examine clinical significance and relativity of heart-type fatty acid binding protein (H-FABP) and high-sensitivity C-reactive protein (hs-CRP) in patients with chronic heart failure. Methods Ser-um concentrations of H-FABP and hs-CRP were measured in 60 patients with chronic heart failure and 30 control subjects. Left ventricular ejection fraction (LVEF) was examined by Doppler echocardio graphic in all subjects. Re-sults Serum concentrations of H-FABP and hs-CRP were higher in patients with chronic heart failure than in con-trol subjects[(6.11±1.49)μg/L vs (4.24±1.40)μg/L,and (12.77±3.65)mg/L vs(4.85±1.35) mg/L,t=5.746 and 7.543,P<0.01] but LVEF was lower in patients with chronic heart failure than in control subjects [(42.13±6.55) % vs (61.50±3.89) %,t=-14.902,P<0.01]. In CHF subgroups,H-FABP and hs-CRP lev-el increased with advancing NYHA class (F=26.288 and 351.784,P<0.01) but LVEF decreased (F=252.834,P<0.01). The serum H-FABP concentrations had a positive correlation with serum hs-CRP concentrations (r=0.801,P<0.01),and a negative correlation with LVEF (r=-0.718,P<0.01) ;serum hs-CRP concentrations had a negative correlation with LVEF(r=-0.881,P<0.01). Conclusion Serum H-FABP and hs-CRP levels are in-creased with the worsening of CHF. H-FABP and hs-CRP level are pnsitiviely related. The quantitative determination of serum concentrations of H-FABP and hs-CRP is valuable for risk stratification in patients with chronic heart fail-ure.     

非诺贝特对高胆固醇血症兔脂肪细胞摄取及降解氧化型低密度脂蛋白的影响

目的　观察兔脂肪细胞通过CD36摄取及降解氧化型低密度脂蛋白(OxLDL)的作用和非诺贝特对高胆固醇血症兔脂肪细胞OxLDL代谢的影响。方法　10只新西兰白兔给予高胆固醇饲料饲养8周后分为: (1)高胆固醇血症组:继续饲以高胆固醇饲料4周; (2)非诺贝特治疗组:在饲以高胆固醇饲料的基础上给予(30mg·kg-1·d-1 )非诺贝特4周。另选饲以普通饲料12周兔5只作为对照组。实验结束后,取皮下脂肪组织行脂肪细胞培养,放射配基法测定脂肪细胞对OxLDL的摄取及降解,RT PCR测定脂肪细胞CD36mRNA的表达。结果　喂饲胆固醇组血清总胆固醇、低密度脂蛋白胆固醇水平明显高于对照组(均P<0. 01),非诺贝特干预4周未对血脂产生影响,但能降低体重( -19%,P<0. 05 )。RT PCR示CD36在脂肪细胞分化过程中被诱导表达。放射配基实验发现兔脂肪细胞呈浓度依赖饱和型的摄取及降解OxLDL,细胞最大结合为2 065ng/mg细胞蛋白,解离常数(Kd)为4. 2mg/L;抗CD36抗体明显抑制脂肪细胞摄取(56% )及降解(54% )OxLDL;当125I OxLDL浓度为75mg/L时,对照组,高胆固醇组,非诺贝特治疗组脂肪细胞摄取125I OxLDL分别为3. 5, 2. 1, 2. 7μg/mg细胞蛋白, 降解125I OxLDL分别为2. 2, 1. 2,1. 7μg/mg细胞蛋白, 3组间差异有统计学意义(P<0. 05)。结论　CD36介导脂     

抑郁对原发性高血压患者血脂的影响

目的探讨原发性高血压患者抑郁发生状况及其与血脂水平之间的关系。方法检测每位入选者的血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL—C）、低密度脂蛋白胆固醇（LDL—C）。用抑郁自评量表（SDS）对每位高血压患者进行抑郁评分,根据评分结果再分为抑郁组、无抑郁组2个亚组,将所有结果进行统计学分析。结果与对照组比较,抑郁组及无抑郁组TG、TC、LDL—C水平均明显升高（均P〈0．05）;HDL—C低于对照组但差异没有统计学意义。在高血压抑郁组、无抑郁组间Tc差异亦有统计学意义[（5．03±1．13）比（5．65±1．69）mmol／L,P〈0．05）];TG、LDL—C水平均差异无统计学意义。相关分析显示SDS评分与TC、TG、LDL—C有负相关趋势但无统计学意义（均P〉0．05）。结论抑郁可能影响血脂代谢,可能是高血压的危险因素。