超临界二氧化碳萃取缬草油气相指纹图谱研究

目的建立超临界二氧化碳萃取缬草油的指纹图谱分析方法。方法采用气相色谱法,以DB-WAX石英毛细管柱为色谱柱,以高纯氮为载气,程序升温法,记录时间为50 min,以氢火焰检测器检测,并对主要共有峰进行了归属。结果用程序升温法得到了各色谱峰较好的分离,确定了27个共有峰。结论为更好地控制缬草药材的内在质量提供了可靠的方法。     

二陈汤配方颗粒与传统煎剂的化学成分比较

目的：采用色谱法对二陈汤配方颗粒剂与传统煎剂中的化学成分进行比较，建立配方颗粒剂的定性鉴别方法。方法：用不同溶剂提取的部位，采用TLC、GC、HPLC及氨基酸分析仪对其配方颗粒与传统煎剂中化学成分进行定性鉴别比较。结果：配方颗粒剂与传统煎剂的色谱图基本一致。结论：此法准确、简便，可作为配方颗粒剂与传统煎剂的鉴别。     

冠状动脉慢性完全闭塞合并远端弥漫性硬化患者的血运重建治疗

Objective To evaluate the effect of off-pump coronary endarterectomy (CE) plus off-pump coronary artery bypass grafting (off-pump CABG) on patients with chronic total occlusion (CTO) combined with diffuse distal atherosclerosis. Methods From October 2006 to August 2009,65 CTO patients with 176 angiographically confirmed vascular stenosis or occlusive lesions, 70 of which were complete occlusion, underwent off-pump CABG. During the operation, diffuse intimal thickening distal to occlusive lesion was found, and blood flow of the bridges was unfavorable.Results Therefore endarterectomy was performed, followed by CABG. The blood flow in the bridges were 2-10 ml/min versus 14-37 ml/min before versus after endarterectomy. Pulsatility index (PI) was 5.1-15.6 versus less than 5 before versus after endarterectomy. Left ventricular ejection fraction was also improved significantly [before operation: (0.47±0.12)%, after operation: (0. 52±0.15)%, t=2.17, P＜0.05]. Peri-operative myocardial infarction occurred in 2 cases, but without significant cardiac homodynamic changes. And 23 patients underwent coronary angiography to evaluate graft patency 3-18 months after operation, all of them had favorable blood flow. Conclusions It is feasible to perform off-pump CABG plus coronary endarterectomy for patients of chronic coronary total occlusion combined with diffuse distal atherosclerosis. This treatment is safe and effective.     

替硝唑药膜治疗智齿冠周炎疗效观察

目的对比观察替硝唑药膜在智齿冠周炎治疗中的疗效。方法选择184例急性智齿冠周炎患者，按是否辅助全身抗生素治疗分为A、B两组，每组分别再随机分为两个小组，一组局部使用替硝唑药膜，另一组常规治疗上碘甘油作为对照组，每组治疗均为1次／d，观察1—5d局部用药的疗效。结果无论是否辅助全身抗生素治疗，替硝唑药膜组疗效明显优于碘甘油组（P〈0．05）。结论替硝唑药膜用于急性智齿冠周炎的治疗疗效可靠。     

高血压患者血TNFα、sIL-2R水平与肾脏早期损害

目的：为探讨高血压患血TNFα.SIL-2R水平与肾脏早期损害的关系。方法：采用ELISA法及化学显色终点法，分别检测40例高血压患血清中肿痛坏死日子(TNFα)、可溶性白细胞介素2受体(sIL-2R)值和尿氨基葡萄糖苷酶(NAG)活力。结果：高血压组TNFα，sTL-2R及尿NAG均明显高于正常对照组，且TNFα、sIL-2R值与NAG活力呈正相关(r=0.84和0.82)。结论：提示高血压患TNFα与肾脏早期损害可能有关．sIL-2R是否参与尚待研究。     

采用分子动力学模拟方法探究Ca~(2+)对VWF-A2结构域稳定性的影响

目的探究Ca~(2+)对VWF-A2结构域稳定性的影响。方法 A2和A2/Ca~(2+)的晶体结构取自PDB数据库。通过恒力拉伸分子动力学模拟,比较分析Ca~(2+)结合引起的构象变化、解折叠路径的差异以及酶切位点的暴露程度。结果 A2结构域的解折叠路径和酶切位点的暴露过程是力依赖的。Ca~(2+)结合不影响A2结构域的前期解折叠,但由于α3β4-环链局部构象重排所致柔性降低,约束了β1-β4-β5片层的运动,导致进一步解折叠受阻而停留在中间稳态,影响酶切位点的充分暴露。结论力可诱导A2结构域中β5片层的解折叠使酶切位点暴露,而Ca~(2+)的结合则通过稳定疏水核心结构,阻碍酶切位点的暴露,最终降低ADAMTS13的酶切效率。研究结果有助于加深对ADAMTS13酶切VWF-A2结构域进而调控VWF止血能力过程的理解,并为相关抗血栓药物设计提供指导。     

臭氧对外科切口杀菌作用的实验研究

为探讨臭氧对外科切口的抗感染作用及对创面愈合的影响,本实验建立动物肛周及背部切口模型,用臭氧水、双氧水及碘伏消毒切口,对比三种消毒剂的消毒效果及对创面愈合的影响。结果显示,臭氧水与双氧水及碘伏相比,其消毒效果更强,创面愈合时间更短。结果表明,臭氧水是一种相对更好的消毒制剂。     

血红素氧合酶1/一氧化碳系统对球囊损伤后内膜增殖及内皮功能的影响

目的 探讨血红素氧合酶1/一氧化碳((HO-1)/CO)系统对兔颈动脉球囊损伤后内膜增殖及内皮功能的影响及其可能的作用机制.方法 家兔随机分为对照组、胆固醇组、血红素组、卟啉锌组和假手术组5组,每组10只.对照组予普通饮食,其余4组喂饲含1.5%胆固醇饲料,血红素组和卟啉锌组同时分别予氯化血红素或锌原卟啉9腹腔内注射,2周后实验组行颈总动脉球囊损伤术,术后续原喂养给药8周.结果 高胆固醇饮食血脂(总胆固醇、甘油三酯、低密度脂蛋白、氧化型低密度脂蛋白)水平显著升高(P<0.01).与对照组比较,胆固醇组颈动脉一氧化氮生成量、cNOS活性显著降低,而一氧化碳生成量、血红素氧合酶活性显著增加(P均<0.01),内膜面积和内膜/中膜面积比值为(0.586±0.090比1.381±0.180).与胆固醇组比较,氯化血红素干预显著增加血红素氧合酶活性、一氧化碳生成量,显著降低内皮素1水平,内膜面积和内膜/中膜面积比值最小(0.386±0.076比0.862±0.164;P均<0.01);锌原卟啉9显著抑制血红素氧合酶活性、一氧化碳生成量,显著增高内皮素1水平,内膜面积和内膜/中膜面积比值最大(0.734±0.096比1.843±0.212),差异均有显著性(P<0.01).结论 高胆固醇饮食加球囊损伤严重损害颈动脉NOS/NO系统,血红素氧合酶1/一氧化碳系统通过代偿和调节NOS/NO系统及降低内皮素1表达从而改善内皮功能,抑制血管损伤后内膜增殖和不良重塑.     

冠状动脉慢性完全闭塞合并远端弥漫性硬化患者的血运重建治疗

Objective To evaluate the effect of off-pump coronary endarterectomy (CE) plus off-pump coronary artery bypass grafting (off-pump CABG) on patients with chronic total occlusion (CTO) combined with diffuse distal atherosclerosis. Methods From October 2006 to August 2009,65 CTO patients with 176 angiographically confirmed vascular stenosis or occlusive lesions, 70 of which were complete occlusion, underwent off-pump CABG. During the operation, diffuse intimal thickening distal to occlusive lesion was found, and blood flow of the bridges was unfavorable.Results Therefore endarterectomy was performed, followed by CABG. The blood flow in the bridges were 2-10 ml/min versus 14-37 ml/min before versus after endarterectomy. Pulsatility index (PI) was 5.1-15.6 versus less than 5 before versus after endarterectomy. Left ventricular ejection fraction was also improved significantly [before operation: (0.47±0.12)%, after operation: (0. 52±0.15)%, t=2.17, P＜0.05]. Peri-operative myocardial infarction occurred in 2 cases, but without significant cardiac homodynamic changes. And 23 patients underwent coronary angiography to evaluate graft patency 3-18 months after operation, all of them had favorable blood flow. Conclusions It is feasible to perform off-pump CABG plus coronary endarterectomy for patients of chronic coronary total occlusion combined with diffuse distal atherosclerosis. This treatment is safe and effective.     

双侧锁骨下静脉变异一例

患者女性,77岁.因"胸闷、心悸、乏力8 h",于2009年4月10日入院.7年前我院心电图提示完全性右束支传导阻滞,3年前在我院行选择性冠状动脉造影未见明显异常.入院心电图提示间歇性三度房室传导阻滞,连续多次心肌坏死标记物检测均为阴性.