除翳明目片联合更昔洛韦眼用凝胶治疗单纯疱疹性角膜炎70例

目的评价分析除翳明目片联合更昔洛韦眼用凝胶治疗单纯疱疹性角膜炎的临床疗效。方法将139例单纯疱疹性角膜炎患者随机分成2组:治疗组78只眼,除翳明目片口服联合更昔洛韦眼用凝胶治疗。对照组77只眼,更昔洛韦眼用凝胶治疗。结果治疗组治愈率85.90%,总有效率96.15%,复发率6.41%,疗程平均(12±5.86)d。对照组治愈率70.13%,总有效率88.31%,复发率16.88%,疗程平均(15±10.12)d。两组治疗结果差异有统计学意义(P0.05)。结论除翳明目片联合更昔洛韦眼用凝胶治疗单纯疱疹性角膜炎疗程短,治愈快,有效率明显增高,复发率明显降低。     

HPLC法测定酸枣仁黄酮片中斯皮诺素

目的建立酸枣仁黄酮片剂中斯皮诺素的测定方法。方法 ODS C18柱(150 mm×3.9μmm,5μm);流动相:乙腈-水(0.1%醋酸),梯度洗脱;体积流量:0.8 mL/min;柱温:25℃;进样量:20μL,检测波长:335 nm。结果斯皮诺素在4~20μg/mL呈良好的线性关系,平均加样回收率为97.98%,RSD为2.58%。结论该方法快速简便、精密度好、灵敏度高,可用于测定酸枣仁黄酮片中斯皮诺素。     

代谢综合征及其组分与前列腺癌发病风险的关系

目的:探讨代谢综合征(metabolic syndrome, MS)及其组分与男性前列腺癌发病风险的关系。方法:基于2014年1月至2016年12月在四川省人民医院健康管理中心进行团队健康体检的在职及离退休男性单位职工作为研究对象,构建健康体检动态队列,通过问卷调查、体格检查、实验室检测等方法收集基线社会人口学特征、MS及其组分指标,随访前列腺癌发病结局,采用Cox比例风险回归模型分析MS及其组分与男性前列腺癌发病风险的关系。结果:293 872例研究对象中,累积随访1 616 296人年,中位随访时间6.15年,随访期间新发前列腺癌病例425例,总体发病密度为26.29/10万人年。在调整年龄等混杂因素后,MS组分分组中,相较于BMI、血压、血脂正常者,超重和(或)肥胖、高血压及血脂紊乱的体检人群其前列腺癌的发病风险明显升高[aRR(95%CI)分别为：1.621(1.330～1.976)、1.868(1.086～3.215)、1.992(1.370～2.896)],同时与非MS患者相比,MS患者前列腺癌的发病风险也明显升高[aRR(95%CI)为：1.998(1.353～2.951...     

杭州市肝癌危险因素的病例对照研究

目的 :探讨原发性肝癌 ( PHC)的危险因素。方法 :对杭州市 10 0例 PHC进行 1∶ 1配对病例对照研究 ,通过条件 L ogistic回归多因素和单因素分析筛选主要危险因素。结果 :结果显示乙型肝炎史与 PHC发生最为密切( OR=18.2 6、95 % CI为 4.40～ 75 .77)。另外 ,肿瘤家族史 ( OR=2 .75 )、心理因素 ( OR=2 .5 1)、胆囊炎 ( OR=5 .0 0 )为 PHC危险因素。结论 :PHC与乙型肝炎史、肿瘤家族史、胆囊炎史和心理因素有关     

玻璃体积血一例误诊

【病例】　男,４１岁。右眼被火炮炸伤１０天,伴视力下降于１９９７年２月１３日到当地医院就诊。查体：右眼视力：指数／１０ｃｍ,左眼１．２;右眼球混合性充血,角膜清,角膜后见白色尘状沉着物,前房深浅正常,房水Ｔｙｎｄａｌｌ现象（＋＋）,瞳孔圆,晶体透明,裂隙灯下见玻璃体内大量色素、灰色颗粒及黄色絮状混浊,眼底窥不见。眼压：右眼５．５／１０＝７．１ｍｍＨｇ,左眼５．５／５＝１７．３０ｍｍＨｇ。诊断：右眼外伤性色素膜炎、视网膜剥脱？２月１４日转某上级医院诊治。先后２次行双眼Ｂ超检查提示：右眼玻璃体内探及…     

刺激声时程对有无噪声暴露史听力正常青年人言语声诱发听性脑干反应的影响北大核心CSCD

目的分析不同时程言语声诱发听性脑干反应(speech evoked auditory brainstem responses,s-ABR)在有无噪声暴露史听力正常青年人中应用的差异,探讨其用于隐性听力损失诊断的临床应用价值。方法选取18~40周岁听阈正常、有噪声暴露史的男性工人15例(接噪组,平均年龄31.53±6.56岁)以及听阈正常、无噪声暴露史的男青年25例(对照组,年龄23.20±4.60岁)进行40 ms/da/音和170 ms/da/音的s-ABR测试,比较两组两种时程刺激声s-ABR潜伏期、波间期和幅值。结果接噪组170 ms/da/音s-ABR的O波潜伏期明显较对照组延长(P<0.05),其余V、A、D、E、F波潜伏期及各波振幅、波间期两组间差异均无统计学意义(均为P>0.05);两组40 ms/da/音s-ABR各波潜伏期、波间期及振幅差异均无统计学意义(P>0.05);对照组170 ms/da/音s-ABR各波潜伏期明显较40 ms/da/音s-ABR延长,各波波间期明显较40 ms/da/音s-ABR增宽(均P<0.05);接噪组170 ms/da/音s-ABR V、A、F、O波潜伏期显著延长(P<0.05),而两种时程刺激声s-ABR各波间期差异无统计学意义(P>0.05)。结论不同时程言语声诱发s-ABR测试可以用于不同的听觉处理机制分析;40 ms时程/da/音的s-ABR各波潜伏期可能对于隐性听力损失的诊断更有价值。     

两种膨润土细胞毒性的体外试验研究

Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite.Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay,neutral red uptake(NRU) assay,lactate dehydrogenase(LDH) leakage assay,apoptosis assay and hemolysis assay.In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0,0.3125,0.6250,1.2500 and 2.5000 mg/ml for ten min.In other four assays,human B lymphoblast cells (HMy2.CIR) served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10,20,30,60,120 and 180 μg/ml for four h.Results In hemolysis assay,the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control (P＜0.05);in CCK-8 assay,the cellular activities in acid bentonite group at the doses ≥30 μg/ml and in organic bentonite group at the doses ≥20μg/ml were significantly lower than that of control (P＜0.01);the similar results appeared in NRU assay and LDH assay,and the dose-effect relationship was observed in above 4 assays.In apeptosis assay,the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control (P＜0.05).Moreover,the results of five in vitro assays indicated the eytotoxicity induced by organic bentonite was higher than that induced by acid bentonite.Conclusion Two kinds of bentonite could induce cytotoxicity,such as apoptosis and damage of cell membrane.The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.     

自制异物钩裂隙灯下摘除角膜深层异物的临床观察

随着乡镇企业迅速崛起，角膜深层异物这个眼科常见病近年来有上升趋势。1991--2004年笔者共收治深层角膜异物103眼，取得较好疗效，现报道如下。