放射线联合p53基因及内皮抑素治疗小鼠前列腺癌皮下移植瘤

Objective To test the hypothesis that p53 gene therapy combined with endostatin can enhance tumor response to radiation therapy of RM-1 mouse xenograft prostate cancer and to investigate its mechanism. Methods A mouse prostate cancer model was established. Then mice with xenograft tumor were randomly divided into group A (control), B (radiation), C (radiation and rAdp53), D (radiation and rh-endostatin) and E (radiation and rAdp53 and rh-endostatin). On day 1, rAdp53 was injected intra-tumorously with 1 × 1010 vp per animal to group C and E. From day 1 to 14, rh-endostatin was given 15 mg/kg intraperitoneally daily to group D and E. On day 4 single fraction of 15 Gy was given to tumors in groups B, C, D and E. Normal saline was injected intra-tumorously or intraperitoneaUy accordingly as control. No treatment was done to group A. Tumor volume was measured daily. Samples were collected on Days 5, 10 and 15. Ki67, CD31, p53 and VEGF were detected by means of immunohistochemistry. Results (1) Radiation alone, radiation combined with intra-tumorous injection of Adp53 and/or intraperitoneal injection of rh-endostatin resulted in tumor growth arrest of RM-1 cells in vivo (P = 0.000). Radiation combined with both rAdp53 and rh-endostatin was the most effective treatment (P < 0.05). (2) All the four treatment groups had a decreased expression of mutant type P53 (P = 0.000). The expression of Ki67 in groups B and C were equal (P 0.05) and increasing (P = 0.000), respectively. Group D had a up-down-up curve (P < 0.05), but group E had a up-down one. On day 5 the expresion of VEGF in group E was the lowest (P < 0.05). An increased expression of MVD compared with the control was shown, and MVD in groups C, D and E were always higher than that in the control (P < 0.05). Conclusions The limitation of radiotherapy could be overcome by combination with beth p53 gene therapy and endostatin on the growth of mouse prostate cancer cell. Radiation, rAdp53 and endostatin have their own role but they can be interacted with each other.     

椎体成形术治疗骨质疏松性椎体压缩骨折的疗效评价

目的 研究椎体成形术在治疗骨质疏松性椎体压缩骨折中的临床效果.方法 选取2018年6月至2020年6月文山州人民医院收治的88例骨质疏松性椎体压缩骨折患者为研究对象,进行回顾性研究.按照随机数字表法将患者分对照组和观察组,各44例.对照组接受常规保守治疗,观察组接受椎体成形术治疗.比较两组治疗总有效率;比较两组患者的骨...     

用侧卧位直接前方入路全髋关节置换术治疗髋关节疾病的早期疗效

目的:探讨采用侧卧位直接前方入路全髋关节置换术治疗髋关节疾病的早期疗效.方法:选取2016年10月至2018年10月期间在文山壮族苗族自治州人民医院进行单侧初次全髋关节置换术的104例髋关节疾病患者为研究对象.根据其手术入路的不同将其分为研究组和对照组.采用侧卧位直接前方入路全髋关节置换术对研究组患者进行治疗,采用后外...