牛蒡子不同炮制品中牛蒡苷含量的HPLC测定

牛蒡子为菊科植物牛蒡Arctium lappa L.的干燥成熟果实。其性辛、苦、寒，归肺、胃经。具有疏散风热，宣肺透疹，解毒利咽等功效。中医临床应用有生品与炮制品之分。牛蒡子传统炮制方法有单炒、酒炒、酒蒸法等，现代以清炒为主。牛蒡苷是牛蒡子的主要有效成分，具有抗肿瘤、抗菌、抗病毒作用及扩     

大鼠脂肪干细胞复合胶原-壳聚糖-硫酸软骨素三维支架构建组织工程软骨

Objective To evaluate the character of the collagen-chitosan-chondroitin sulfate scaffold seeded with rat adipose tissue-derived stromal cells. Methods A dipose tissue were harvested from 6 weeks old Wistar rats and the stromal cells were harvested by type Ⅰ collagenase and then cultured in vitro. Type Ⅰ collagen was fully mixed with chitosan, freeze-dried and cross-linked with chondroitin sulfate, then freeze-dried again and sterilized by ethylene oxide. The pore diameter, water content, porosity of the scaffold were tested. The adipose tissue-derived stromal cells were digested, seeded into the plates, scaffold, and cen-trifuged into pellet, and then induced into cartilage. MTT detection for cell proliferation was done. After 3 weeks, the cell morphology, and cell proliferation and adhesion were observed, and chondrngenic differenti-ation was also analyzed. Results The pore diameter, water content, porosity tested for the scaffold showed an appropriate form. Cell proliferation showed faster in the scaffold and pellet culture system after 5 day, there was still cell proliferation in the scaffold system after 14 days but no obvious changes in the pellet cul-ture system; ceils on the scaffold proliferated densely showed by histological staining, but there was a scaf-fold structure residues in the inner layer. The finding of type Ⅱ immunohistochemistry stain showed that cells express strong positive for type Ⅱ collagen in the scaffold and pellet culture system whereas it was weakly positive in the plate culture system; the specific mRNA for cartilage, type Ⅱ collagen, aggrecan and SOX-9 were expressed in all three systems showed by RT-PCR, but type X collagen was expressed continu-ously in the plate culture system and expressed after 21 days in the pellet culture system, whereas it was not detected in the collagen-chitosan-chondroitin sulfate scaffold system. Conclusion The parameters of the collagen-chitosan-chondroitin sulfate scaffold were suitable in our study. The results suggested that it can promote the adipose tissue-derived stromal cells proliferation and chondrogenic differentiation better than the plate and pellet culture systems and maintain the phenotype of chondrocytes well; it is the optimal choice for cartilage tissue engineering in the future.     

4例颈静脉球瘤的影像学诊断

目的：分析颈静脉球瘤的各种影像学表现。重点讨论此瘤各种影像学检查的价值与限度。方法：报道4例经手术病理证实的颈静脉球瘤，并结合文献对其影像学表现及其各种检查方法进行分析。结果：颈静脉球瘤的影像学检查方法颇多；其表现各具特点，尤其是高分辨率薄层CT及MR表现颇具特征性，能为此瘤术前诊断提供可靠依据。结论：颈静脉球瘤的各种影像学检查方法都有一定的价值和限度。应恰当选择，合理应用，可取得理想的诊断结果。尤其是高分辨率薄导CT及MR检查具有明显的优势，应作为术前常规和必需的检查项目。     

夏津县机动车驾驶员4355名健康体检结果分析

了解我县机动车驾驶员的健康状况 ,为交通职业安全管理提供依据 ,笔者对 1998～ 2 0 0 2年本县 43 5 5名机动车驾驶员的职业健康检查结果进行了分析 ,现将结果报告如下。1　内容与方法按照《山东省机动车驾驶员职业健康检查试行方案》的要求进行。检查对象为本县各种机动驾驶员。2　结果与分析2 1　职业禁忌证检出情况　全县共查机动车驾驶员 43 5 5人 ,全部为男性 ,查出职业禁忌证 3 0 2人 ,检出率为 6 93 %。其中高血压 78人 ,色觉异常 70人 ,心电图异常 69人 ,视力不良 3 6人 ,听力下降 3 5人 ,其他职业疾患 14人 ,检出率依次为 1 79%、…     

雷尼替丁加大剂量维生素C治疗过敏性紫癜

过敏性紫癜是一种主要侵犯毛细血管的变态反应性疾病,是血管炎综合征中的最常见类型。过敏性紫癜的临床特点除皮肤紫癜外,常有皮疹、关节肿痛、腹痛、便血和血尿等。传统治疗方法是用苯海拉明、钙剂、肾上腺皮质激素等抗过敏治疗。1998-2000年,用雷尼替丁加大剂量维生素C治疗过敏性紫癜37例,收到显著疗效,现报道如下。     

颈椎间盘突出症的髓核突出类型及其临床意义

目的对颈椎间盘突出症进行分型,并探讨其临床意义。方法手术治疗颈椎间盘突出症110例,根据MRI特点分为Ⅰ型,Ⅱ型和Ⅲ型,并就分型和临床的关系,选择手术方式。结果两节段以下的各型突出,含有Ⅱ型或Ⅲ型突出的多节段颈椎间盘突出,伴动力性颈椎不稳的各型突出,均应首选前路手术;三节段以上的Ⅰ型突出,发育性颈椎管狭窄合并Ⅱ型或Ⅲ型突出,应选择后路手术。结论颈椎间盘突出症的分型对手术入路选择有指导意义。     

脑结核瘤延误诊断二例

1 病例资料 [例1] 女,18岁.1993年3月不明原因出现头痛,呈进行性加重,伴乏力、恶心、呕吐,呕吐呈喷射状,呕吐物为胃内容物,无发热、盗汗、咳嗽、咳痰.     

膀胱三角区注射肉毒素A治疗膀胱过度活动症不会引起膀胱输尿管返流

膀胱过度活动症是一种以尿急症状为特征的症候群,常伴尿频和夜尿症状,可伴或不伴急迫性尿失禁.抗胆碱能药物抑制逼尿肌收缩是主要的治疗手段,但疗效不理想,且不良反应较大.膀胱镜下于膀胱内逼尿肌散在注射肉毒素A是一种新的治疗方法,但目前认为注射区域不应该包括三角区,以避免术后膀胱输尿管返流.