ERβ调节海马依赖性高级认知功能的研究进展

<正>雌激素受体(estrogen receptor,ER)是核受体转录因子超家族的成员。经典ER有两种亚型,由于它们在结构、功能及表达上具有很多相似性,组成上有一定程度的同源性,故习惯上按发现时间定义为ERα和ERβ。雌激素受体可与特异性配体结合发挥生物学作用。海马位于人类和其他灵长     

三亚疗养地疗养因子对亚健康状态康复调节的作用

本院对来我院疗养的215例亚健康疗养员观察,三亚疗养地疗养因子对亚健康状态康复效果良好,现报告如下。1资料与方法1.1一般资料对2008年1月-2012年4月来武警三亚疗养院疗养的疗养者入院后进行健康体检的同时进行亚健康评估,随机抽取215名亚健康者为观察组。其中     

慢性乙型肝炎患者中医证型与生存质量及心理障碍相关性分析

目的:探讨慢性乙肝中医证型与患者生存质量及心理障碍的相关性,为慢性乙肝的中医辨证施治和心理干预工作的开展提供科学依据。方法:随机抽取168例诊断为慢性乙肝患者作为研究对象进行中医辨证分型,并采用慢性肝病问卷(CLDQ)进行生存质量测评,研究各证型分布特点,以及与抑郁自评量表(SDS)、焦虑自评量表(SAS)评分差异。结果:(1)中医证型分布以肝郁脾虚证和肝胆湿热证比例最高分别为36.9%和28.6%。(2)CLDQ问卷以肝郁脾虚、肝胆湿热两型评分最低(P0.05)。(3)中医各证型神经症评分比较,肝胆湿热证总积分最高、肝郁脾虚证次之(P0.05);且不同证型在症状积分分布上具有明显的差异性(P0.01或P0.05)。(4)SDS、SAS量表评分比较:SDS评分以肝郁脾虚最高(P0.01),SAS评分以肝胆湿热证最高(P0.01)。结论:慢性乙肝中医证型分布与患者生存质量、心理障碍有一定的相关性,以肝胆湿热和肝郁脾虚型心理障碍最为突出。     

加强门诊部监督管理 确保人民安全用药

随着经济发展和社会文化水平的不断提高，市民用药知识的不断丰富，自己根据病情到社会门诊部（所）诊治取药逐渐增多。但是，社会门诊部分散，进药渠道杂乱，药品量少，增加了药品监督管理工作难度。近几年我们根据本市的具体情况（我市其中有三个区无药检所）采取了一些...     

二氮嗪预处理联合低温对大鼠海马神经元缺氧复氧时Bcl-2和Bax表达的影响

Objective To investigate the effects of diazoxide preconditioning combined with hypothermia on the expression of Bcl-2 and Bax during anoxia-reoxygenation in rat hippocampal neurons. Methods The hippocampal neurons isolated from newborn SD rats ( < 24 h, weighing 5-6 g) were inoculated in the culture dish or 96 well plates. The hippocampal neurons were randomly assigned into 8 groups and each group contained 36 wells or 12 dishes of neurons: normal temperature .group (group NT), diazoxide preconditioning (DP) + NT group (group DP+ NT), mild hypothermia group (group MiH) , DP + MiH group (group DP + MiH) , moderate hypothermia group (group MoH), DP + MoH group (group DP + MoH), deep hypothermia group (group DeH) and DP+DeH group (group DP+ DeH). In group DP + NT, DP + MiH, DP+ MoH and DP + DeH, diazoxide was added to the culture media, the final concentration was 100 μmol/L,and the neurons were incubated for 1 h once a day for 2 d, and then subjected to 4 h of hypoxia at 37, 34, 30 and 22℃ , respectively, followed by 48 h of reoxygenation at 37℃ . The neuronal viability, apoptotic rates and expression of Bcl-2 and Bax were determined and the ratio of Bcl-2/Bax was calculated. Results The neuronal viability was significantly higher, apoptotic rate lower, Bcl-2 expression higher, Bax expression lower and Bcl-2/Bax ratio higher in group DP + NT, MiH, MoH and DeH than in group NT ( P < 0.05). The neuronal viability was significantly higher, early apoptotic rate lower, Bcl-2 expression higher, Bax expression lower and Bcl-2/Bax ratio higher in group DP + MiH, DP + MoH and DP+ DeH than in group DP + NT ( P < 0.05), but there was no significant difference in the late apoptotic rate between group DP + MiH, DP + MoH, DP + DeH and DP + NT ( P > 0.05). The neuronal viability was significantly higher, early apoptotic rate lower, Bcl-2 expression higher, Bax expression lower and Bcl-2/Bax ratio higher in group DP+ MiH and DeH than in group MiH (P < 0.05), but there was no significant difference in the late apoptotic rate between group DP + MiH, DeH and MiH, and no significant difference in the above indices between group MoH and MiH (P > 0.05) . The neuronal viability was significantly higher, early apoptotic rate lower, Bcl-2 expression higher, Bax expression lower and Bcl-2/Bax ratio higher in group DP+ DeH than in group DP+ MiH ( P < 0.05). Conclusion Diazoxide preconditioning combined with hypothermia can attenuate the anoxia-reoxygenation injury in rat hippocampal neurons possibly through correcting the imbalance of Bcl-2 and Bax and inhibiting the early apoptosis of hippocampal neurons.     

二氮嗪预处理联合低温对大鼠海马神经元缺氧复氧时Bcl-2和Bax表达的影响

Objective To investigate the effects of diazoxide preconditioning combined with hypothermia on the expression of Bcl-2 and Bax during anoxia-reoxygenation in rat hippocampal neurons. Methods The hippocampal neurons isolated from newborn SD rats ( < 24 h, weighing 5-6 g) were inoculated in the culture dish or 96 well plates. The hippocampal neurons were randomly assigned into 8 groups and each group contained 36 wells or 12 dishes of neurons: normal temperature .group (group NT), diazoxide preconditioning (DP) + NT group (group DP+ NT), mild hypothermia group (group MiH) , DP + MiH group (group DP + MiH) , moderate hypothermia group (group MoH), DP + MoH group (group DP + MoH), deep hypothermia group (group DeH) and DP+DeH group (group DP+ DeH). In group DP + NT, DP + MiH, DP+ MoH and DP + DeH, diazoxide was added to the culture media, the final concentration was 100 μmol/L,and the neurons were incubated for 1 h once a day for 2 d, and then subjected to 4 h of hypoxia at 37, 34, 30 and 22℃ , respectively, followed by 48 h of reoxygenation at 37℃ . The neuronal viability, apoptotic rates and expression of Bcl-2 and Bax were determined and the ratio of Bcl-2/Bax was calculated. Results The neuronal viability was significantly higher, apoptotic rate lower, Bcl-2 expression higher, Bax expression lower and Bcl-2/Bax ratio higher in group DP + NT, MiH, MoH and DeH than in group NT ( P < 0.05). The neuronal viability was significantly higher, early apoptotic rate lower, Bcl-2 expression higher, Bax expression lower and Bcl-2/Bax ratio higher in group DP + MiH, DP + MoH and DP+ DeH than in group DP + NT ( P < 0.05), but there was no significant difference in the late apoptotic rate between group DP + MiH, DP + MoH, DP + DeH and DP + NT ( P > 0.05). The neuronal viability was significantly higher, early apoptotic rate lower, Bcl-2 expression higher, Bax expression lower and Bcl-2/Bax ratio higher in group DP+ MiH and DeH than in group MiH (P < 0.05), but there was no significant difference in the late apoptotic rate between group DP + MiH, DeH and MiH, and no significant difference in the above indices between group MoH and MiH (P > 0.05) . The neuronal viability was significantly higher, early apoptotic rate lower, Bcl-2 expression higher, Bax expression lower and Bcl-2/Bax ratio higher in group DP+ DeH than in group DP+ MiH ( P < 0.05). Conclusion Diazoxide preconditioning combined with hypothermia can attenuate the anoxia-reoxygenation injury in rat hippocampal neurons possibly through correcting the imbalance of Bcl-2 and Bax and inhibiting the early apoptosis of hippocampal neurons.     

脑缺血神经元凋亡的机制

脑缺血（cerebral ischemia）是危害人类生命健康的疾病，病理机制十分复杂，近几年对其机制的探讨已不断深化到细胞、分子水平。越来越多的论据表明，细胞凋亡（apoptosis）在脑缺血中发挥着重要的作用。     

miR-196a在下咽鳞状细胞癌血浆中的表达及其临床意义

目的探讨mi R-196a在下咽鳞状细胞癌(HSCC)患者血浆中的表达水平及其与下咽癌临床因素的关系,明确mi R-196a的诊断价值及临床意义。方法利用57例临床确诊HSCC患者血浆配对样本,健康体检者血浆样本50例,从血浆标本中提取总RNA,应用实时荧光定量PCR(quantitative real time PCR,q RT-PCR)方法检测mi R-196a在血浆中的表达水平,收集57例HSCC患者病理、临床资料,统计分析血浆mi R-196a水平和临床病理特征的关系。构建受试者工作特征曲线(receiver operating characteristic curve,ROC),探讨血浆mi R-196a的诊断价值。结果在HSCC患者中术前mi R-196a的水平9.11(5.69,12.43)显著高于术后12.69(9.16,14.84)及健康体检者12.85(11.18,14.91)(P<0.01);血浆标本中HSCC患者术后mi R-196a水平与健康体检者比较无显著差异(P>0.05)。mi R-196a血浆中的表达水平和分化程度(P<0.05)、淋巴结转移(P<0.01)、临床分期(P<0.05)有显著相关性;与年龄、吸烟史无关(P>0.05)。ROC曲线下面积为0.755,截断(cut-off)值为△Ct=10.84,95%CI:0.66~8.85(P<0.01)。结论血浆mi R-196a血浆表达水平与HSCC患者的肿瘤分期、淋巴结转移、病理类型等若干临床特征相关。mi R-196a有望作为HSCC微创诊断分子标志物。     

rFN／CDH—BCP组织工程骨的制备和体内融合效应的初步研究

目的制备复合bMSCs的rFN／CDH—BCP组织工程骨,并对其体内融合效应进行初步观测。方法制备复合bM-SCs的rFN／CDH—BCP组织工程骨,利用细胞离心粘附实验、MTT实验和成骨诱导分化等方法观察组织工程骨界面对骨种子细胞的粘附和分化的影响。建立兔腰椎横突间植骨融合模型,以复合bMSCs的rFN／CDH-BCP组织工程骨植于预备的骨槽内,利用X线影像学和组织学技术观察融合部位新骨生成状况及种子细胞分布、转归状况。结果bMSCs复合24h后rFN／CDH—BCP表面和内部有大量细胞粘附,生长良好,成骨诱导10d后,rFN／CDH-BCP表面ALP活性显著升高（P〈0．05）,21d后,钙结节广泛分布。bMSCs复合rFN／CDH-BCP植入兔植骨融合区12周后X线和组织学显示有明显新生骨生成,对照组没有。结论rFN／CDH—BCP组织工程骨在体内外均有良好的生物学效应,对新骨形成和骨成熟具有显著的促进作用和良好修复作用,具有较广阔的临床应用前景。     

奥曲肽联合普萘洛尔治疗肝硬化上消化道出血患者效果及对炎性指标水平和门、脾静脉血流影响

目的观察奥曲肽联合普萘洛尔治疗肝硬化上消化道出血患者的效果及对炎性指标水平和门、脾静脉血流的影响。方法选取肝硬化上消化道出血78例,根据治疗方法不同将其分为奥曲肽联合普萘洛尔组(43例)和奥曲肽组(35例)两组。奥曲肽联合普萘洛尔组采用醋酸奥曲肽联合盐酸普萘洛尔治疗,奥曲肽组仅采用醋酸奥曲肽治疗。观察比较两组治疗3 d后临床效果,治疗前及治疗3 d后血清炎性指标水平及门静脉血流量(PVF)、脾静脉血流量(SVF),以及治疗期间不良反应发生情况。结果治疗3 d后,奥曲肽联合普萘洛尔组总有效率高于奥曲肽组,差异有统计学意义(P0.05)。治疗前,两组血清炎性指标水平及PVF、SVF比较差异均无统计学意义(P0.05)。治疗3 d后,两组血清C反应蛋白(CRP)、白细胞介素6(IL-6)水平及PVF、SVF均较治疗前降低,血清白细胞介素10(IL-10)水平均较治疗前升高;且奥曲肽联合普萘洛尔组血清CRP、IL-6水平及PVF、SVF低于奥曲肽组,血清IL-10水平高于奥曲肽组,差异有统计学意义(P0.01)。治疗期间,两组不良反应总发生率比较差异无统计学意义(P0.05)。结论奥曲肽联合普萘洛尔治疗肝硬化上消化道出血患者可提高临床效果,减轻炎症反应,改善门、脾静脉血流情况,且安全性良好。