神经信息学与针刺机理研究

针灸的理论基础是经络，针灸临床治疗时的辨证归经、循经取穴、针刺补泻等，无不以经络理论为依据。近几十年来，国内外许多专家学者结合现代多种学科知识，综合运用各种现代科技手段，从不同的角度对针刺机理及经络的实质提出了种种假说，进行了有益的探索。但是，目前对针灸治病的作用机理尚不完全清楚，经络的实质问题尚未得到解决。神经科学和信息科学是当今自然科学研究的两大热点。     

益肾达络饮对实验性自身免疫性脑脊髓炎IFN-γ、IL-4的影响

采用ELISA方法,观察益肾达络饮对PLP139151诱导的高敏感品系SJL小鼠自身免疫性脑脊髓炎的症状、中枢神经系统内细胞因子IFNγ、IL4的影响。结果发现益肾达络饮对自身免疫性脑脊髓炎小鼠保护作用可能与抑制局部炎症,促进Th1/Th2比例平衡,有一定关系。     

"数字化北京老年疾病防控体系"的建立

“数字化北京老年疾病防控体系”是一套比较完善的老年健康协同服务体系，是以促进老年健康和老年社会和谐为宗旨、以中国老年康保网为平台、以老年健康档案与诊疗信息动态管理系统为主线、以专家预约挂号系统为特点、以市或区县级老年病医院及社区卫生服务中心（站）为试点的老年医疗保健信息化管理体系，是由北京老年医院、国家医药卫生数据共享工作组、天健公司和新奥博为公司共同创建的老年疾病三级防控体系。     

老年功能性失眠整体辨治心得

功能性失眠是大多数老年人都会经历的睡眠——醒觉模式的生理变化，现代医学指排除心脑疾患、躯体及情感原因，患者连续3周以上入睡难、易醒及（或）多梦、晨醒早等表现的睡眠障碍，多伴见头晕头痛、心悸乏力、健忘等症状。     

脑疏宁对脑损伤大鼠脑微血管保护作用的实验研究

目的：研究脑疏宁对脑微血管的保护作用。方法：建立大鼠脑外伤模型，观察脑微血管通透性、超微结构及分布密度的变化。结果：大鼠脑伤后6h开始，脑微血管结构破坏，通透性增加、密度下降；中药治疗组微血管通透性降低，密度增高，结构较完整。结论：脑疏宁保护损伤区脑微血管结构和功能完整性，增加皮质微血管的数目。     

补阳还五汤治疗缺血性中风的研究进展

补阳还五汤是清代名医王清任根据其气虚血瘀理论而创立 ,全方由 7味药组成 ,君药黄芪生用四两大补元气之虚 ,而奏益气行瘀之功 ;臣药以当归尾 2钱 ,活血祛瘀而不伤正 ,轻用桃仁、红花、川芎、赤芍、地龙为佐使 ,以活血化瘀通络。全方配伍严谨 ,气血并顾 ,标本兼顾 ,独具匠心。近年来本方的临床应用及实验研究不断深入 ,丰富了本方的治疗领域 ,尤其是本方临床治疗中风的广泛应用 ,无疑为补阳还五汤作用的研究注入了新的生命力。1　临床研究对于缺血性中风的认识大多数学者认为 ,气虚血瘀为重要病机 ,气虚是本病之本 ,血瘀乃本病发生发展的核…     

肝气郁结证相关脑区的正电子发射断层成像

目的:观察肝气郁结证整体水平上脑功能变化的特点,获得肝气郁结证中枢病变的定位信息.方法:采用配对研究的原则,应用正电子发射断层扫描成像技术,选择典型肝气郁结证患者60例,应用SPM图像分析软件,对比分析肝气郁结证与正常志愿者脑功能影像学的不同.结果:左侧额下回(BA 47)、扣带回前部、扣带回后部、颞中回(BA 21)、额中回(BA 6)、双侧颞上回(BA 37,38)、中央前回(BA 4,6)等表现为葡萄糖低代谢状态(P＜0.01);脑干中脑、延桥交界、右侧楔叶(BA 18)、左侧小脑表现为葡萄糖高代谢状态(P＜0.01).结论:肝气郁结证相关脑区多呈现葡萄糖利用率降低,整体水平上为中枢参与肝气郁结证病变提供了客观的可视性影像学依据.     

Effects of free radicals and amyloid β protein on the currents of expressed rat receptors in Xenopus oocytes

Objective To investigate the effects of free radicals (FRs) and amyloid β protein 1-40 ( Aβ(1-40)) on the functions of expressed neurotransmitter receptors (NRs) i n Xenopus oocytes.Methods Total RNA and messenger RNA (mRNA) was prepared from 3-month-old Wistar rat br ain tissues with Promega kits and microinjected into maturated Xenopus oocyt es (stages Ⅴ-Ⅵ) with 50 nl (50 ng) for each oocyte. The microinjec ted oocy tes were incubated with modifiedBath’s solution at 19.0℃±1.0℃ for receptor expression and their currents were recorded with double electrode voltage clamp technique. Superoxide anion free radicals (SAFRs) were produced via a reaction system (HPXXO) with hypoxanthine (HPX, 0.05 mol/L) and xanthine oxidase (XO, 0.1 U/L). In order to observe the effects of Aβ and SAFRs on the e xpressed glutamate receptor, HPX/XO and Aβ(1-40) were added to incubation solution at 12 h, 24 h and 96 h before recording.Results The results showed that the oocytes expressed functional NRs originating from ra t brain tissues. These NRs included muscarinic acetylcholine (mACh), glutamate (Glu), dopamine (DA), serotonin (5-HT) and γ-aminobutyric acid (GABA). T he c urrent characteristics of expressed receptors were inward currents carried by ch loride ion with their equibrilium potentials close to -22 mV. The extent of ef fect on the current of expressed glutamate receptor from rat brain was different among different Aβ concentrations and incubation times. Aβ(1-40) at a c oncentration of 20 nmol/L had little effect on the currents of expressed rat br ain glutamate receptors up to 24 h of incubation period; but the currents of gl utamate receptor were significantly decreased (25% off, P<0.01) in the trea tment of 60 nmol/L Aβ(1-40) over 24 h. Moreover, when 20 nmol/L Aβ (1-40) was co-incubated over 12 h with SAFRs produced by the reaction syste m of HPXXO, it was found that the currents of expressed rat bra in glutamate rec eptors had been changed markedly. When the oocytes were co-treated with 60 nm ol/L Aβ(1-40) and SAFRs over a period of 12 h, the currents of glutamate receptor significantly decreased (21% off, P<0.05), and the decreased perce ntage reached 52% over 24 h co-treatment with 60 nmol/L Aβ(1-40) and SA FRs. In addition, vitamin E had a partial effect against this inhi bitory effect.Conclusion The results suggest that Aβ has a kind of inhibitory effect upon the current of the glutamate receptor, similar to the effects of free radicals. The effects c an be antagonized by vitamin E. These imply that Aβ may play a role via inhibi ting receptor function in the pathophysiology of Alzheimer’s disease.     

血糖水平及再灌流对大鼠局部脑缺血影响的实验研究

本文用Ｗｉｓｔａｒ大鼠８０只，尼龙线堵塞法建立可再灌流的急性脑缺血动物模型，随机分为闭塞和再灌流两大组。根据血糖水平不同，每组又分为５个亚组。术后测定神经功能及梗塞灶体积。发现再灌流组高血糖和重度低血糖大鼠较正常血糖大鼠神经功能评分低，梗塞灶大，统计学均有显著差异。非再灌流组，只有重度低血糖大鼠较正常血糖大鼠神经功能评分低，梗塞灶大，其它各组之间神经功能评分和梗塞灶体积均无显著差异。结果表明，高血糖和重度低血糖加重脑缺血性损伤，而且血糖与局部脑缺血的影响与再灌流密切相关     

人脑初级体感皮质M20反应的性别差异-脑磁图研究

目的应用脑磁图(MEG)研究健康受试者初级体感皮质反应M20的潜伏期、波幅及位置的性别差异.方法对40例受试者给予双侧腕部正中神经电刺激,固定电流脉冲0.3ms,刺激间期0.5s,诱发初级体感皮质兴奋.脑磁图检查后进行MR扫描,层厚1.5mm.大脑初级体感皮质反应由等电流偶极(ECD)评价.将脑磁图所获得的m20反应叠加到MRI上,形成磁源性影像(MSI).结果依照给予电刺激后反应潜伏期不同分别称为M20、M35及M60,因M35及M60易受刺激间期(ISI)的影响,只对M20的潜伏期、波幅及反应位置的性别差异进行分析.结果为(1)M20左、右半球潜伏期男性20.6±1.0ms和20.7±1.2ms,女性20.1±0.9ms和20.2±0.9ms,男女性同侧半球潜伏期无显著性差异(P＞0.05);(2)M20左、右半球波幅男性23.6±11.2nAm和25.4±11.2nAm,女性25.8±10.5nAm和27.6±7.8nAm,男女性同侧半球波幅无显著性差异(P＞0.05);(3)左半球男女性M20偶极位置在X轴位存在显著性差异(P＜0.05),显示男性X轴上与女性相比稍偏外侧,右半球男女性M20偶极位置在X轴位无显著性差异(P＞0.05),y,Z轴无显著性差异(P＞0.05);(4)磁源性影像显示所有受试者M20反应峰值均位于中央后回.结论脑磁图可明确不同性别的大脑初级体感皮质反应M20潜伏期、波幅及位置.磁源性影像显示M20峰值位于中央后回手区体感皮质.