内皮依赖性超极化因子的研究进展

内皮依赖性超极化因子是由内皮细胞释放并作用于平滑肌细胞的非一氧化氮、非前列环素途径的血管舒张因子,其作用机制复杂,可通过钙激活钾通道开放、缝隙连接传递电化学信号以及内皮释放等方式作用于平滑肌细胞,最终导致血管舒张。高血压、糖尿病等血管性疾病的发生发展可能与内皮依赖性超极化因子相关,其有望成为心血管疾病治疗的新靶点。     

针药结合治疗重度神经性聋1例

神经性聋是指内耳听觉神经、大脑的听觉中枢发生病变,而引起听力减退甚至听力消失的一种病症,常常伴有耳鸣.一般病程短者治疗效果明显,病程超过3个月则预后较差.兹将针药结合治疗重度神经性聋1例临证经验介绍如下.     

针刺人迎穴调控RhoA/ROCK1通路改善自发性高血压大鼠血压及血管损伤的机制研究

[目的]基于RhoA/ROCK1通路探讨针刺人迎穴对自发性高血压大鼠血压及血管损伤的作用机制。[方法] 11周龄雄性自发性高血压大鼠,随机分为模型组、针刺组和西药组,选取同周龄雄性Wistar大鼠作为正常组。针刺组和西药组分别给予针刺人迎穴和厄贝沙坦灌胃给药。观察大鼠血压变化情况,酶联免疫吸附实验(ELISA)检测血管紧张素Ⅱ(AngⅡ)和ROCK1的表达水平,逆转录聚合酶链反应(RT-PCR),蛋白免疫印迹法(Western Blot)检测内皮素-1(ET-1)和人血小板衍化生长因子-AA(PDGF-AA)的mRNA和蛋白表达水平,免疫荧光检测颈动脉组织中基质金属蛋白酶2(MMP2)和RhoA的定位及表达水平。[结果] 1)与模型组比较,西药组和针刺组在治疗3周,治疗6周时的平均动脉压有降低(P＜0.05)。2)与模型组比较,西药组和针刺组在治疗6周时ET-1和PDGF-AA的mRNA和蛋白的表达均降低(P＜0.05)。3)与模型组比较,西药组和针刺组在治疗6周时RhoA、AngⅡ和ROCK1的蛋白表达均降低(P＜0.05),针刺组在治疗6周时MMP2的蛋白表达降低(P＜0.05)。4)与西药组比较,针刺组在治疗6周时AngⅡ和ROCK1的蛋白表达均降低(P＜0.05)。[结论]针刺人迎穴可能通过下调颈动脉RhoA/ROCK1通路来改善自发性高血压大鼠血压及血管损伤。     

“醒脑开窍”针刺法对脑缺血大鼠脑皮质UCH-L1表达的影响

目的：探讨脑缺血大鼠皮质泛素C末端水解酶L1（UCH—L1）的表达以及“醒脑开窍”针刺法的干预作用。方法：采用大鼠大脑中动脉阻塞（MCAO）模型并通过western blotting方法检测醒脑开窍针对组、对照组、模型组,假手术组UCH—L1的表达。结果：6h时,醒脑开窍针刺组、对照组和假手术组与模型组相比较UCH—L1的表达都降低,醒脑开窍针刺组减低最明显;24h时．醒脑开窍针刺组和对照组与模型组比较仍有显著的降低。结论：UCH—L1可能参与脑缺血损伤,“醒脑开窍”针刺法能够拮抗大鼠脑缺血后UCH—L1的高表达．效果优于传统针刺法。     

“醒脑开窍”针刺法对脑缺血大鼠脑皮质Peroxiredoxin Ⅵ表达的影响

目的 探讨脑缺血大鼠皮质抗氧化蛋白Ⅵ(peroxiredoxin Ⅵ)的表达以及"醒脑开窍"针刺法的干预作用.方法 采用大鼠大脑中动脉阻塞(MCAO)模型,通过western blotting方法 ,检测醒脑开窍针刺组、对照组(传统针刺组)、模型组,假手术组Peroxiredoxin Ⅵ的表达.结果 6h时,醒脑开窍组与模型组比较表达明显升高(P<0.05);24h时,醒脑开窍组和对照组与模型组比较都有显著的升高(P<0.05).结论 结果 提示"醒脑开窍"针刺法能够提高大鼠脑缺血后Peroxiredoxin Ⅵ的表达,在缺血早期效果优于传统针刺法,对提高大鼠脑缺血后的抗氧化能力有积极意义.     

膜片钳技术在高血压研究中的应用

膜片钳技术（patch-clamp technique）是1976年由Nehetr和Sakroann在电压钳的基础上发展而来的一种记录细胞膜离子通道电生理活动的技术。通过微电机与细胞膜之间形成紧密接触,采用电压钳或电流钳技术对生物膜上离子通道的电活动进行记录。膜片钳技术的应用将细胞水平和分子水平的生理学研     

当归羊肉生姜汤在肝癌介入治疗后的应用体会

我们在肝癌介入术后立即应用当归羊肉生姜汤治疗，能够明显改善症状、提高患者生存质量，同时也增强了患者对进一步治疗的耐受性。     

miR-9通过调控β-tubulin Ⅲ、GFAP对神经干细胞增殖的影响

目的 探究miR-9是否通过调控β-微管蛋白-Ⅲ(β-tubulinⅢ)、胶质纤维酸性蛋白(GFAP)影响神经干细胞(NSCs)的增殖.方法 对健康孕鼠进行NSCs分离培养并将分离出的细胞均分为NO组(无转染NSCs细胞株)、NN组(NSCs转染miR-9-NC)、NM组(NSCs转染miR-9 mimics)、NI组...     

电针水沟穴抗轻、中度休克:多中心随机对照研究

OBJECTIVE: To probe into the effect of electroacupuncture (EA) at Shuigou (GV 26) on mild and moderate shock. METHODS: With 3-center randomized control study method, 276 cases were assigned to an EA plus medicine group and a medication group, 138 cases in each group. They were treated respectively with western medicine plus EA at Shuigou (GV 26), and simple western medicine. Their curative effects were observed after treatment for 6 hours. RESULTS: The blood pressure was immediately increased in the EA plus medicine group and the increase of blood pressure was earlier than that in the medication group (P<0.001). The markedly effective rate of 52.9% in the EA plus medicine group was significantly higher than 18.1% in the medication group (P<0.001). CONCLUSION: The therapeutic effect of EA at Shuigou (GV 26) plus western medicine on mild and moderate shock is better than that of simple western medicine.     

World Integrated Medicine Master SHI Xuemin (石学敏)

"It is my eternal responsibility to inherit acupuncture and moxibustion well"Shi Xuemin—Academician Shi Xuemin,National Master of Traditional Chinese Medicine,representative inheritor of Representative Projects of National Intangible Cultural Heritage"Some people say that I am a magic needle with a ghost hand,while others say that the silver needle diplomacy.In fact,what I want to do most is to pass on the traditional Chinese acupuncture and moxibustion technology and make this Traditional Chinese Medicine(TCM)skills benefit more people."In the face of so many accolades,Shi Xuemin values the word"inheritance"most."To carry forward acupuncture and moxibustion well,so that TCM can better serve the Chinese people and the people all over the world,"Shi said.Inheritance is also the source of the continuous development of representative intangible cultural heritage projects.A small silver needle is passing from Academician Shi Xuemin to the younger generations.These silver needles,relayed through his disciples,so that more patients'health is protected.In the process of passing on,we will let such a heart of love light up the lives of more people.