乏氧HeLa细胞的超微结构

实体瘤的血管网来之于邻近正常组织,因此,自瘤表面至瘤体中心随着血管密度减低而呈现氧浓度梯度。通常瘤体中心细胞处于无氧状态而坏死,坏死灶边缘细胞处于极度乏氧状态,称为乏氧细胞。这种常处于静止期的乏氧肿瘤细胞对物理、化学治疗皆不敏感,在一定条件下,可重入细胞周期活跃增殖,致使肿瘤复发。本文报道乏氧的人体子宫颈癌HeLa细胞系细胞的超微结构特征,为探讨乏氧对人癌细胞     

THE LABORATORY AND CLINICAL STUDIES OF SULFONAMIDES

This paper reports the result.s of ant.ibac- terial, pharmacologic and clinical studies on the sulfonamides, trimethoprim (TMP) and sulfona- mide-TMP combinations manufactured in China. Bacterial sensitivity tests of these drugs were carried out on 3,549 strains of pathogens, belong- ing t0 18 bacterial species, isolated clinically. When combined with TMP, the antibacterial ac- tivities of the sulfonamides were greatly eh- hanced particularly on Staph. au'reus, E. coli, Enterobacter aerogenes and proteus spp, which were approximately similar to those of kana- mycin or gentamycin. Under similar conditions the blood and urine concentrations of 7 different kinds of sulfonamides and TMP administered orally, intramuscularly, and intravenously were determined in a group of healthy volunteers. The data indicate that sulfadiazine (SD), which for a long time had been considered as a short-acting drug and its dosage was greater than actually needed, is a medium-acting sul- fonamide. In our study, SD was used in re- duced dosage in the treatment of various infec- tions, and favorable clinical results were obtain- ed with much fewer side effects. Sulfonamides alone and their combination with T'MP were used in 2,486 patients of typhoid fever, pneumo- nia, bacillary dysentery, acute and chronic urinary infections etc., the effective rates being 83.3To and 92.5To respectively. It is better to use sulfonamide compounds in patients suffering from various infections with the exception of meningococcal meningitis. In more severe ca.ses, it is advisable to take the drug by parenteral routes at the start, in order that effective serum levels may be reached as early as possible. Pre- liminary data indicated that SD compound used instead of SMZ compound in various clinical sitiuations is just as effective if not mor'e so. Both the two sulfonamide compounds are com- monly used in our clinics at present.     

棉酚对大鼠~(42)K代谢影响的研究

本实验用同位素~(42)钾(~(42)KGl)示踪放射自显影定位以及定量测定方法研究棉酚对大鼠钾代谢的影响及其与低血钾的可能关系。动物连续喂服棉酚(剂量为5～10毫克/日/鼠)4-17周后按体重从舌下静脉注射50～100微居里~(42)KGl溶液。于注射后不同时距冷冻动物制成放射自显片观察,另取心、肾、骨骼肌、血和尿进行γ线闪烁定量测定。实验结果表明:(1)~(42)K注入体内后迅速与体内钾交换而摄入于脏器组织中,其中肾、心、脾、胰及胃肠道管壁出现放射活性高峰的时间最早,摄入速度最快。以下依次为软骨、肌肉、肝、皮下脂肪、肺、皮肤及睾丸,而以红细胞和脑脊组织的摄入速度最慢。血浆~(42)K的放射强度在注入体内30分钟后即降至较心、肾和肌肉为低的相对恒定水平。(2)大鼠服棉酚后,各脏器和血液中~(42)K的单位放射强度、摄入速度和分布动态,一般与对照组无明显差异,但肾脏的尿排~(42)K率则有增高倾向。其中少数动物在尿~(42)K排出率增高的同时,血浆和脏器~(42)K均明显下降。提示棉酚引起肾性失钾的可能性。本文对棉酚影响K代谢及引起肾性失钾的可能机理予以分析和讨论。     

动物Na—K—ATP酶活性变化与棉酚相关的研究——Ⅱ、长期喂服棉酚对大鼠骨Na—K—ATP酶影响的电镜观察

<正> 棉酚是我国首创的一种男性节育药,其抗生育作用已在临床及动物实验中得到证实。但有少数人服药后出现低血钾肌无力症。各实验室从不同方面观察了棉酚与低血钾的关系,并阐明了各自的观点。我们曾分别观察了对猴、大鼠、豚鼠喂服不同剂量棉酚的急性实验,而本文报道长期喂服棉酚对大鼠肾Aa-K-ATP酶影响的电镜观察。     

高血压脑出血术后二次脑损伤因素与脑组织氧及预后的关系研究

目的研究高血压脑出血术后合并二次脑损伤因素对患者脑组织氧及预后的影响。方法对2006年1月-2013年6月收治的120例高血压脑出血患者行手术治疗,术后对影响二次脑损伤的血压、体温、动脉血氧分压、血糖、血钠等多项指标进行监测,其中10例同时行脑组织氧监测,将患者按是否存在二次脑损伤因素分为阳性组和阴性组,并比较两组术后格拉斯哥预后分级。结果 73例阳性组患者格拉斯哥预后分级为Ⅴ级13例,Ⅳ级23例,Ⅲ级11例,Ⅱ级0例,Ⅰ级26例;47例阴性组分级为Ⅴ级19例,Ⅳ级16例,Ⅲ级4例,Ⅱ级2例,Ⅰ级6例;阳性组预后差于阴性组,差异有统计学意义(Z=-3.241,P=0.001)。脑组织氧监测显示脑灌注压、动脉血氧分压均与脑组织氧分压呈正相关(P<0.001)。结论二次脑损伤因素的异常改变,影响患者的脑组织氧代谢,可造成二次脑损害,继而影响预后。     

大鼠肺缺血再灌注组织microRNA-451与蛋白激酶B表达

目的探讨大鼠肺缺血再灌注组织中microRNA-451及蛋白激酶B（p-AKT）的表达及二者之间的相关性。方法将Wistar大鼠随机分为对照组、缺血组、再灌注1h组、再灌注3h组及再灌注6h组,每组10只,采用实时荧光定量PCR（RT-qPCR）及蛋白免疫印迹法（Western blot）检测各组microRNA-451及p-AKT蛋白的表达。结果与对照组比较,缺血组、再灌注1h组、再灌注3h组、再灌注6h组microRNA-451的相对表达量呈现先上调后下调趋势,其中再灌注3h组microRNA-451表达量最高,差异有显著性（F=17 244.32,P〈0.05）。各组p-AKT蛋白相对表达量比较,对照组及再灌注6h组较高,再灌注3h组最低,差异有显著性（F=136.02,P〈0.05）。结论大鼠肺缺血再灌注过程中microR-451可能通过调节AKT蛋白通路促进细胞凋亡。     

广州市2001-2010年婴儿死亡率趋势分析及预测

婴儿死亡率(IMR)是衡量一个国家或地区国民健康和经济发展水平的重要指标.本文通过趋势分析探讨广州市2001 -2010年IMR的变化趋势并预测未来10年的水平,旨在为制定进一步降低IMR的政策措施提供依据.