表达端粒酶逆转录酶siRNA的溶瘤腺病毒对裸鼠肾癌移植瘤的治疗作用

Objective To investigate the antitumor effect of oncolytic adenovirus armed with small interference RNA targeting hTERT gene for renal cancer therapy. Methods Nude mice were divid-ed randomly into 4 groups (8 mice/group),and were treated by intratumoral injections of ZD55-hTERT ( an oncolytic adenovirus armed with small interference RNA targeting hTERT gene) ,ZD55-EGFP ( an on-colytic adenovirus) and Ad-hTERT (replication-defective adenovirus armed with small interference RNA targeting hTERT gene) with three consecutive daily at 7 × 108 pfu/day or treated with PBS as a control. The expression of E1A and hTERT, and apoptosis of tumor xenografts were assessed by immunohistochemi-cal technique at the 7th day after injections. The tumor volume was measured at the 50th day after injec-tions. Results The tumor volume in ZD55-hTERT treatment group ( 124.1±27.5) was significantly less than that in ZD-EGFP (499.8±77.1 ) and Ad-hTERT ( 609.0±102.5 ) treatment groups. The E 1A pos-itive expression in ZD55-hTERT treatment group was significantly higher than that in Ad-hTERT treatment group. The hTERT positive expression in ZD55-hTERT treatment group was significantly lower than that in Ad-hTERT treatment group. ZD55-hTERT treatment of tumor xenografts resulted in an increased apoptotie cell death as compared with ZD55-EGFP and Ad-hTERT treatment. Conclusion The antitumor effect of ZD55-hTERT was more potent than oneolytie adenovirus ZD55-EGFP and Ad-hTERT.     

硬膜外麻醉后神经并发症

自 Pages(1921)及 Dogliotti(1927)经腰椎行硬膜外麻醉后,30年未发现严重神经后遗症,曾有作者建议用它替代脊麻。但1952年以后,陆续发表一些麻醉后截瘫的病例报道,1975年经 Usubiaga 收集,共有69例硬膜外麻醉后严重神经并发症,致病原因有:损伤,注射药物刺激,感染,因脊髓血管病变等。总的说,硬膜外麻醉后神经并发症仍属罕见,Dawkins复习大量文献,暂时性轻瘫发生率约0.1%,永久性截瘫为0.02%。下文分别讨论几种常见的硬膜外麻醉后神经并发症。     

面中骨折术后眶下神经损伤恢复的临床观察

目的：观察面中骨折后眶下神经损伤的恢复状况。方法：对28例面中骨折后眶下神经损伤的患者,术后通过患者自述、针剌检测、两点辨别觉及直流感应电测仪测定眶下神经的恢复状况及恢复时间。采用SPSS12．0软件包对数据进行t检验。结果：25例患者的眶下神经损伤得到恢复,神经恢复时间在4-6个月,平均25周：3例患者未能恢复．成为永久性损伤。未发现慢性神经性疼痛患者。结论：大部分骨折后眶下神经损伤是暂时的、可恢复的,极少数为永久性损伤。对伤后6个月神经功能仍未恢复的患者,可考虑行眶下神经减压术。     

不同椎间隙硬膜外术后镇痛效果的比较

随着对术后疼痛病理认识的提高,人们已将术后镇痛视为提高生活质量,促进患者术后早日康复的重要环节。与传统肌内注射麻醉性镇痛药相比,患者硬膜外自控镇痛技术(PCEA)具有镇痛效果好,用药量少,血药浓度维持恒定,可根据个体对镇痛药的不同需求而投放用药等优点,在临床上已广泛开展,取得了良好的镇痛效果。本研究通过对2010年1月至2011年5月140例不同椎间隙穿刺留置硬膜外导管术后镇痛的效应进行了探索比较,报告如下。     

沙利度胺联合氟尿嘧啶、表阿霉素治疗中晚期肝癌临床观察

原发性肝癌在我国有较高的发病率及死亡率,日前临床多为中晚期患者,总体疗效有限.解放军一五九中心医院自2008年2月至2010年1月对30例中晚期原发性肝癌患者应用抗血管生成药物沙利度胺Thd联合表阿霉素、亚叶酸钙、氟尿嘧啶(FE方案)静脉化疗,疗效较理想.资料与方法一、临床资料选取解放军一五几中心医院2008年2月至2010年1月30例原发性肝癌患者,均为不能或不愿行手术、介入治疗,或手术或介入治疗后疾病进展者.年龄31～65岁,平均45岁;男21例,女性9例;初治10例,复治20例;Ⅲ期22例,Ⅳ期8例,其中6例伴肝 门淋巴结肿大,5例伴 门静脉癌栓,4例肺转移;1例伴脑转移;4例反复发作低血糖反应;10例并发腹水.