Inhibitory Effect of Extract of Fungi of Huaier on Hepatocellular Carcinoma Cells

This study investigated the inhibitory effect of the extract of fungi of Huaier (EFH) on the growth of hepatocellular carcinoma (HCC) cells.Hep-G2 cells,a human HCC cell line,were cultured in DMEM containing 10% fetal bovine serum and treated with EFH of different concentrations (1,2,4,8 mg/mL) for 24,48 and 72 h respectively.The apoptosis rate of the cells was flow cytometrically measured.Thirty-six tumor-bearing New Zealand rabbits were randomly divided into 3 groups:group A (control group),in which the rabbits were infused with 0.2 mL/kg normal saline via the hepatic artery;group B (transhepatic artery chemoembolization [TACE] group),in which the rabbits were given lipiodol at 0.2 mL/kg plus MMC at 0.5 mg/kg via the hepatic artery;group C (TACE +EFH group),in which EFH (500 mg/kg) were orally administered after TACE.Two weeks after TACE,the rabbits were sacrificed and the implanted tumors were sampled.The tumor volume and the necrosis rate were determined.The tumor tissues were immunohistochemically detected for the expressions of factor Ⅷ,VEGF,P53,Bax and Bcl-2.The microvessel density (MVD) was calculated by counting the factor Ⅷ-positive endothelial cells.Our results showed that after treatment with EFH,the apoptosis rate of Hep-G2 cells was enhanced in a concentration- and time-dependent manner.Two weeks after the treatment,the average tumor volume,the necrosis rate and the growth rate of the implanted tumor in group C were significantly different from those in groups A and B (P<0.05).MVD and VEGF expressions were significantly decreased in the group C when compared with those in groups B (P<0.05 for all).The Bax expression was weakest in group A and strongest in group C.The expressions of P53 and Bcl-2 were minimal in group C and maximal in group A.There were significant differences in the expressions of P53,Bax and Bcl-2 among the 3 groups (P<0.05 for all) and there was significant difference between group B and group C (P<0.05).It was concluded that EFH could suppress not only the growth of HCC cells but also tumor angiogenesis and it can induce the apoptosis of HCC cells.EFH serves as an alternative for the treatment of HCC.     

载药栓塞微球经肝动脉化疗栓塞术联合阿帕替尼治疗不可切除肝癌后二线追加卡瑞利珠单抗的安全性和有效性分析

目的 分析在载药栓塞微球经肝动脉化疗栓塞术(DTACE)联合阿帕替尼治疗不可切除肝癌后,二线追加卡瑞利珠单抗的安全性和有效性。方法 回顾性分析2019年12月—2020年12月于郑州大学第一附属医院就诊的89例二线追加卡瑞利珠单抗的肝癌患者资料。主要观察终点是使用卡瑞利珠单抗后总生存期(OS)和无进展生存期(PFS),次要终点包括客观缓解率(ORR)、疾病控制率(DCR)和治疗相关不良事件(TRAE)。采用Kaplan-Meier法估算生存曲线,基线特征亚组分层分析采用Log-rank检验进行比较,分析影响患者预后的相关因素。结果 本研究共筛选并随访了89例患者。随访至2021年12月,中位随访时间为16个月,中位OS为17.0(95%CI:15.3～18.7)个月,中位PFS为7.0(95%CI:6.2～7.8)个月。不同ECOG-PS、肝功能Child-Pugh分级、门静脉侵犯、进展模式、DTACE次数、口服阿帕替尼时长和应用卡瑞利珠单抗时长的患者OS及PFS之间差异具有统计学意义(P值均<0.05)。应用卡瑞利珠单抗后3个月与6个月ORR分别为39.3%和22.4%,DCR...     

1259例河南省Budd-Chiari综合征分布分析

目的了解河南省Budd-Chiari综合征患者的临床流行病学特点,探讨该病病因及发病机制。方法收集2003年1月~2010年5月郑州大学第一附属医院、第二附属医院及河南省人民医院连续诊治的1 259例Budd-Chiari综合征的临床资料,进行描述性统计学分析。结果河南省Budd-Chiari综合征患者分布有以下特征：男女构成比为58.9∶41.1;平均年龄为（41.14±11.88）岁;各年龄段均有患病,不论男女均以青壮年患病为主;在职业构成上,农民占绝大多数（82.3%）;农村明显多于城镇;常年发病,无明显的季节波动;豫东多,豫西、豫北少,平原多于山区;病变类型以混合型（75.9%）居多。结论河南省Budd-Chiari综合征形成的病因可能与环境因素有关。     

DSA引导下经T管调管并二次置入胆道引流管治疗肝胆术后胆汁漏4例

目的探讨利用导丝调整异常T管的位置并二次置入胆道引流管治疗肝胆手术术后胆汁漏的有效性及安全性。 方法回顾性分析2018年1月至2020年1月于我院在DSA引导下经T管调管并二次置入胆道引流管治疗肝胆术后胆汁漏的4例患者资料。经T管引入导丝导管,导丝导管配合,调整异常的T管并进入肝内胆管内,经导丝引入8.5F COOK胆道外引流管置于肝内胆管,行胆道负压外引流。总结评价治疗效果。 结果术后4例患者负压引流管引流通畅,胆汁漏逐渐停止,腹腔内感染得到控制。术后1个月复查造影,见引流管位置正常,无造影剂外溢至腹腔,2个月拔除T管及胆道引流管后,患者无特殊不适。随访1年,患者病情未再反复。 结论DSA引导下经T管调管并二次置入胆道引流管是治疗肝胆手术术后胆汁漏的安全有效的方法,可避免再次外科手术,减轻患者痛苦。     

CT导引下经皮胸部穿刺活检术的技术研究

目的：讨论总结CT导引下经皮胸部穿刺活检术的技术特点及临床应用。方法：采用Picker1200SX专家型CT扫描仪，SIEMENS Somaton Balance螺旋CT扫描仪，各种型号抽吸针及切割针对72例胸部占位病变患者行CT导引下经皮活检术，病变直径1.5～15.6cm。结果：穿刺成功率97．2%(71／72)，活检阳性率87．5％％(63／72)并发症：气胸11．0％(8／72)血胸5．6％(4／72)咯血4．2％(3／72)均无须作特殊处理。结论：CT导引下经皮胸部穿刺活检术穿刺成功率及活检阳性率较高，并发症较少、症状轻，对胸部占位病变的诊断、鉴别诊断具有重要的临床意义，值得临床推广应用。     

经皮肝穿刺胆道腔内射频消融联合胆道支架治疗恶性胆道梗阻的临床观察

目的：探讨经皮肝穿刺胆道腔内射频消融技术姑息性治疗恶性梗阻性黄疸的安全性及可行性。方法选取2013年1月－2014年3月接受治疗的20例无法切除的恶性梗阻性黄疸患者作为实验组,其中9例不能外科手术切除患者透视下行经皮肝穿刺胆道腔内射频消融术,并留置金属胆道支架;同期随机纳入11例相似病情患者作为对照组,经皮肝穿刺胆道造影（PTC）成功后仅行胆道支架置入。观察两组手术并发症及黄疸缓解情况,密切随访并比较术后3和6个月支架的通畅性。结果所有患者术后通过门诊或电话均获得完整随访。实验组及对照组术后3个月支架通畅率分别为100％,72．7％（χ2＝2．888,P ＝0．218）,术后6个月支架通畅率分别为：87．5％、27．3％（χ2＝6．739,P ＝0．02）。随访期内射频组1例患者于术后113 d 死于消化道出血,对照组1例患者于57 d 死于肝功能衰竭,1例患者于术后142 d 死于弥散性血管内凝血。结论经皮肝穿刺胆道腔内射频消融对恶性胆道梗阻的治疗是安全和可行的,在延长自膨式金属支架通畅性方面初步疗效令人满意,但尚需大量样本随机对照研究进一步验证。     

短视频在介入放射学教学中的应用

【摘要】 目的 评价短视频在介入放射学教学中的效果。 方法 研究对象为来自郑州大学医学院影像系2019级本科生82人,实验组41人,对照组41人。实验组在常规PPT基础上联合短视频完成授课,对照组采用传统PPT授课。用随堂测验和学生满意度调查评估教学效果。结果 实验组随堂测试分数高于对照组,为（84.9 ± 6.3）分比（78.5 ± 5.8）分（P＜0.01）,问卷显示在授课方法、学习兴趣、知识接受度、图像生动性等方面,实验组学生与对照组比较,差异均有统计学意义（均P＜0.05）。结论 以短视频教学能够丰富教学手段,配合传统教学方法,可获得良好教学效果。