Survivin反义核酸对拓扑替康诱导SKOV3细胞凋亡的影响

目的 :探讨Survivin反义核酸对拓扑替康 (TPT)诱导卵巢癌细胞SKOV3凋亡的影响。方法 :在光镜下观察Survivin反义核酸与TPT联合应用后SKOV3凋亡的形态学改变 ,通过DNA电泳检测SKOV3细胞凋亡的情况 ;应用MTT法检测SKOV3细胞对TPT的敏感性。结果 :Survivin反义核酸可明显促进TPT诱导的SKOV3凋亡 ,呈现出特征性的DNA梯状带 ;TPT对SKOV3、SKOV3/neo、SKOV3/SVVas细胞的IC50 值分别为 (1 6 0 .0 0± 6 .36 ) μg/L、(1 5 8.0 0±6 .32 ) μg/L、(4 2 .0 0± 2 .31 ) μg/L ,其差异具有统计学意义 (P <0 .0 1 )。 结论 :Survivin反义核酸可明显促进TPT诱导的卵巢癌细胞SKOV3凋亡 ,提高卵巢癌细胞对TPT的敏感性     

宫颈鳞癌组织中EphA2基因的表达

目的探讨宫颈鳞癌组织中EphA2的表达.方法应用免疫组化法观察EphA2在206例FIGO Ⅰ a-Ⅳb期的宫颈鳞癌组织中的表达,从中选择20例不同EphA2蛋白表达的病例,应用激光捕获显微切割技术(Laser cap-ture microdissection,LCM)从冰冻组织切片中选切大约50个宫颈鳞癌细胞,应用RT-PCR方法检测EphA2mRNA的表达.结果206例宫颈鳞癌组织中,EphA2蛋白阴性表达23例(11.2%),弱阳性表达94例(45.6%),阳性表达72例(35.0%),强阳性表达17例(8.3%);EphA2蛋白与其mRNA表达水平不完全一致;EphA2蛋白表达与宫颈鳞癌患者的年龄、FIGO分期及淋巴结转移无关,总生存期在EphA2染色强度0～3级之间差异有统计学意义(P＜0.05).结论EphA2在宫颈鳞癌中的高表达预示患者的生存差.EphA2有望成为抗宫颈癌治疗的新靶点.     

5-氟尿嘧啶诱导人淋巴细胞免疫耐受的作用观察

药物诱导免疫耐受中,用环磷酰胺（ｃｙｃｌｏｐｈｏｓ－ｐｈａｍｉｄｅ,ＣＰ）诱导最具代表性,它是一种选择性杀伤增殖细胞ＤＮＡ的抗细胞有丝分裂药物。将供体小鼠的脾细胞（１０８）注射到受体小鼠体内,２天后将２００ｍｇ／ｋｇ的ＣＰ注射给受体小鼠,就可以成功地...     

高危型人乳头瘤病毒载量与子宫颈病变的关系

Objective To determine the association between viral load of high risk human papillomavirus (HR-HPV) and cervical intraepithelial neoplasia (CIN). Methods Cervical exfoliated cells were collected from 18 186 women aged 17 -59 from six urban areas and eight rural areas when they were screened in the cross-sectional population-based studies from 1999 to 2008. HR-HPV was detected by the Hybrid Capture 2 (hc2) system, and viral load was measured by the ratio of relative light units to standard positive control (RLU/PC). RLU/PC was categorized for analysis into four groups: negative [0, 1.00),low viral load [1.0, 10.00), moderate viral load [10.00, 100.00), and high viral load 100. 00. Cervical lesions were diagnosed by biopsies as normal, CIN 1, CIN 2, CIN 3 and squamous cervical cancer (SCC). Association between HR-HPV viral load and CIN was evaluated by unconditional multinomial logistic regression. Results The HR-HPV infection rate of the population was 14. 51% (2515/17 334). 100. 00% (29/29) of SCC,97. 63% (206/211) of CIN 3,93.43% (199/213) of CIN 2,75.04% (421/ 561) of CIN 1 and 10. 17% (1660/16 320) of normal women were positive for HR-HPV DNA. The median RLUs for the HR-HPV positive women with SCC,CIN 3,CIN 2,CIN I and normal were 320. 85,158. 05, 143. 70,125.34 and 9. 64, respectively. There were significant differences among the distributions of viral loads in each lesion (X2=6190. 40,P<0. 01). The severity of CIN increased with the viral load (X2=5493. 35 ,P<0. 01). Compared with the risks of CINs in HR-HPV negative population,the risks of CINs in low,moderate and high viral loads were increased gradually [OR(95% CI) : CIN 1 : 9. 01 (6. 31 -12. 87), 24.96(18.23 -34. 17) and 68.42(51.40 -91.08); CIN 2:26.44(12.07 -57.95),98. 53 (49. 54 -195.98) and 322. 88(168.62 -618. 27) ; CIN 3 + : 72. 89(24.02 -221.18) ; 343. 58(121.81 -969.09) Was 3115.05,2413.95 and 3098.57, respectively. P<0.01) . In each age group of the HR-HPV positive population,the risks of CIN 2 + in the women with moderate or high viral load were higher than the one with low viral load [OR(95%CI):<35: 4. 71(1.23-18.09) and 15.06(4.40-51.49); 35-: 4.01 (1.62-9.90) and 14.09(6. 15 -32.28); 40-: 3.06(1.52 -6. 16) and 7.78(4.05 -14.95); ≥45: 3. 50(1.36 -9. 01) and 7. 57 (3. 13 -18. 30)], and there was a positive correlation between the risk of CIN 2 + and the viral load (Xtrend2was 51. 33,66. 28,53. 64 and 51.00,respectively. P<0. 01). The risk of CIN 2 + was highest among the women aged 40 -with high viral load [0R(95% CI):2.02 (1.15 -3. 52)]. Conclusion There is strong correlation between the HR-HPV viral load and the severity of CIN, and so is the correlation between the HR-HPV viral load and the risk of CIN 2 +. A moderate to high viral load of HR-HPV should be the major risk factor for the cervical cancer and CIN 2 and CIN 3,and there is a higher risk in the women aged 35 or older than the younger ones. Considering both the age and viral load could help the doctors to manage the screening women more effectively.     

树型分类及其应用

目的 探讨树型分类方法在流行病学队列资料分析中的应用价值。方法 依据人群的同质性将总人群根据某一变量（在流行病学分析中,通常为危险因素）分成不同的亚人群（在不同的亚人群中疾病的发生根率不同）。结果 各危险因素在疾病发生中的作用、作用方式、危险因素间的交互作用的估计;疾病高危人群的筛选。结论 树型分类对多元分类资料进行分析时,不涉及参数推断,原理简单,尤其适用于有共线性的资料处理。在疾病的危险因素分     

山西省榆社县改善生育卫生需求评估——参与性农村评估调查总结

１９９８年１１月,国家卫生部改善生育卫生服务山西课题组运用参与性农村评估（ＰＲＡ）对山西省榆社县经济状况好,中、差的３个行政村进行了参与性生育卫生服务需求评估调查。调查结果表明：（１）产前检查、新法接生、产后访视等常规孕产期保健服务的利用较差,妇女生育健康水平较低;（２）孕产妇及其丈夫,家属均缺乏孕产期保健、妇女生殖道感染等生育健康知识,同时她／他们也迫切需要了解这些知识。建议：（１）加强健康教育     

酒精摄入量与云锡(YTC)矿工肺癌的队列研究

为了解酒精摄入量与云锡９ＹＴＣ）矿工肺癌危险性的关系,在食物频率问卷（ＦＦＱ）资料中饮酒重复性和有效性研究的基础上,计算出云锡矿工肺癌高危人群酒精摄入量;用ｘ＾２检验、单因素、多因素Ｃｏｘ回归分析了云锡矿工肺癌高危人群不同酒精摄入量与其发生肺癌相对危险性的关系。结果表明：酒精摄入量与云锡矿工肺癌发病率呈“Ｊ形”关系,即不饮酒和中等量以上饮酒者（酒精摄入量≥５０ｇ／ｄ）,肺癌发病率显著高于少量饮酒（     

214例腰椎间盘脱出症手术远期疗效观察

目的:观察腰椎间盘脱出症手术的远期疗效.方法:对手术后3年以上的214例进行随访分析.结果:术后优良率92.1%.存在的主要问题是腰腿疼痛、麻木,性功能障碍,排尿排便无力.结论:(1)选择合适的手术方式以减少腰椎不稳、硬膜外及神经根周围纤维化.(2)早期功能练习,提高腰背肌功能.     

有移位髌骨骨折的术中分型及分型标准

目的：提出有移位髌骨骨折的术中分型及分型标准,探讨其与内固定方法的选择。方法：通过对３３２例有移位髌骨骨折患者的手术观察,按术中骨折部位及骨折块碎裂大小程度,将其分为Ⅰ－Ⅴ种类型,根据术中分型选择合理的内固定方法。结果：术后随访６个月－７年,按宁氏疗效评定标准评定,优良率达９１％。结论：有移位髌骨骨折的术中分型及分型标准具有全面,准确,简明和实用等优点,利于术中内固定方法的选择及预后判定,具有重要     

胃镜营养管留置在胃切除术后重度吻合口水肿中的运用及评价

目的 :探讨胃镜营养管留置治疗胃大部切除术后重度吻合口水肿。方法 :采用 Olympus GIF- P2 0前视胃镜 ,对 42例重症吻合口水肿患者 ,进行十二指肠内营养管留置疗法。结果 :41例患者取得疗效 ,其中 ,留置一次治疗成功者 39例 ( 92 .2 % ) ,留置二次治疗成功者 2例 ( 4.7% ) ,1例患者 ( 2 .3% )自行拔管而失败。结论 :十二指肠营养管留置 ,治疗胃切除术后重度吻合口水肿引起的呕吐、营养障碍等疗效可靠 ,并发症少 ,优越性强 ,值得推广