Metabolism of [14C]arachidonic acid by polymorphonuclear leukocytes in patients with psoriasis

The formation of LTB4 and its omega-oxidation products 20-hydroxy- and 20-carboxy-LTB4 from exogenous [14C]arachidonic acid (AA) by neutrophils from 12 psoriatic patients and 10 healthy controls was investigated. Only a slight difference was detected in the mean amount of [14C]LTB4 produced. In contrast, the amounts of [14C]omega-oxidation products obtained from psoriatic PMN were 2.4-fold higher than the amounts from PMN of healthy controls. We conclude that in vitro, psoriatic PMN synthesize more LTB4 from exogenous AA than do PMN of healthy individuals and due to an efficient omega-oxidation system, the net release of LTB4 in both groups appears to be similar.     

嗜铬细胞瘤患者围手术期处理的改进

目的：探讨提高嗜铬细胞瘤患者围手术期安全性的措施。方法：对15例嗜铬细胞瘤患者、术前采用选择性α1受体阻滞剂多沙唑嗪控释片(可多华)控制高血压；11例患者采用3D DCEMRA进行肿瘤定位；术中均采用联合高容量血液稀释(AHH)和术中患者自体血回输纠正血容量。结果：除1例持续性高血压患者需联合钙离子拮抗剂和ACEI药物降压外．其余患者血压控制均为满意；根据3D DCEMRA定位选择手术径路者均顺利完成手术；AHH联合术中自体血回输使14例患者避免了异体输血。结论：本文围手术期处理改进．可多华可明显减少药物的不良反应；采用3D DCEMRA进行肿瘤定位，可增加手术安全性；AHH联合术中自体血回输可减少医疗成本和异体输血，以及由此而引起的并发症。     

BIOEQUIVALENCE BETWEEN rhGH FOR RECONSTITUTION AND READY-TO-USE rhGH IN TWO LIQUID FORMULATIONS

Objective To evaluate the bioequivalence between recombinant human growth hormone （rhGH） for reconstitution, and two dosages of liquid formulation of rhGH [ （151U） 5mg or （301U） lOmg per 3ml ]. Methods The study drugs were tested in a randomized, single-blind and three-period crossover studies in 24 healthy male subjects. The three drugs were administered by subcutaneous injection at a dose of O. 21U/kg body weight. A continuous somatostatin infusion was given in order to suppress the secretion of endogenous GH. The ve- nous blood samples were drawn at different time points to test the serum concentration of GH. The pharmacokinetic parameters were analyzed by statistical methods. Results 90% confidence intervals （CI） of AUC0-24h among three products were all within 80% - 125% interval （ 103. 4% - 116. 5%, 105. 7% - 119. 6% and 91.9% - 103. 7%, respectively）, and the Cls of C,~ among three products were all within 70% - 143% interval （91.9% - 114. 0%, 103. 7% -127. 2% and 81.6% -97. 4%, respectively）. There was no statisitical difference of tmax among all the three products. Conclusion These data demonstrate that there is bioequivalence between rhGH for reconstitution and two liquid formulations of rhGH.     

硫酸锌对大鼠移植胰腺再灌注损伤的保护作用

Objective:To study the protective effects of the heat shock protein 70 （HSP70）induced by zinc sulfate on reperfusion injury following pancreaticoduodenal transplantation in rats.Methods :The homologous male Wistar rat model of heterotopic total pancreaticoduodenal transplantation was used. The ZnSO4 treated rats received the intravenous injection of Zn2+5 mins before and after operation at a dose of 5 mg/kg (Zn 1 group),10 mg/kg(Zn 2 group) and 15 mg/kg(Zn 3 group), and the control group with the same volume of saline. The tissue concentration of HSP70 was determined using Western Blot. In addition，blood sugar (BG) and serum concentration of amylase and lipase were examined 24h after transplantation, and the activity of myeloperoxidase (MPO) in the pancrease graft was measured at the same time. Histological observation was performed.Results:Light microscopic studies showed that histomorphological changes of pancreas in Zn 2 group and Zn 1 group were much less than those in control group and Zn 3 group. The value of BG and serum lipase and MPO in Zn 2 group ＜ Zn 1 group 相似文献   

Caspase-3反义寡核苷酸抗凋亡作用的研究

目的 研究caspase-3反义寡核苷酸对6-OHDA诱导大鼠中脑多巴胺神经元凋亡的保护作用.方法 向四组SD大鼠的中脑黑质部位注入反义、错义、正义寡核苷酸及NS,然后再注入6-OHDA,取中脑做连续切片,原位杂交及免疫组化检测黑质caspase-3的表达及TH的表达,原位末端标记法(Tunel)检测黑质细胞的凋亡.结果 反义组Tunel阳性细胞数为82±8.6,方差分析显示反义组阳性细胞数显著性低于其他各组(P＜0.05);反义组手术侧TH阳性细胞数为168.6±11.4,与对侧阳性细胞比值为63%±11.3%,显著性高于其余各组(P＜0.05).结论 有效阻断caspase-3的表达可减轻6-OHDA诱导的多巴胺神经元凋亡,caspase-3可作为保护性治疗帕金森病的靶点.     

尿液分析标准化在临床上的应用

目的：建立尿液分析的标准化。方法：NCCLS和CCCLS推荐方法。结果：按标准化的要求进行尿液分析，以获得有临床价值的结果。结论：尿液分析是临床最常用的化验指标之一，在临床诊治疾病中起着非常重要的作用，因此在实际工作中必须遵照标准化的操作规程进行操作，更好地为临床服务。     

急性心肌缺血的动态关联维数

已有的研究成果表明，冠心病患者心脏各导联心电图(ECG)的关联维数D2比正常人均有不同程度的下降。为了进一步研究心肌缺血情况、导联位置和关联维数变化的关系，本研究设计了6只兔子急性心肌缺血实验模型，计算了其不同时间段的12导同步ECG，作出关联维数一时间(D2-T)曲线，结合电生理和解剖学知识，研究了实验结果，发现急性心肌缺血初期ECG的D2值具有动态变化特性，其原因在于心血管中存在代偿作用。ECG的D1的变化反映了单一化的心脏传输网络系统的非线性复杂性的变化。将此结论由动物推广到人，可以为临床上运用D2值分析心肌缺血病症严重程度提供理论指导。     

上腹部不对称联体双胎一例

患儿男,4 d.因联体畸形于2008年3月17日入院.体检:体重共3 kg.正常侧婴儿无明显外部畸形,其胸骨柄的下方剑突部至脐的上方有一畸形体与之相连(图1).畸形体(寄生体)仅有四肢、腹部、臀部、男性外生殖器,有尿从阴茎自主排出.寄生体与正常侧婴儿相连接处,长12.0 cm,宽5.5 cm,周长30.0 cm,连接处两腹腔相通.寄生体四肢无自主运动,对刺激无反应,能做被动关节运动.     

肢体负压对周围动脉闭塞性病变犬皮肤SP免疫反应阳性神经纤维影响

目的　观察肢体负压对周围动脉闭塞性病变犬皮肤中 SP免疫反应阳性神经纤维的影响 .方法　犬 17只 ,随机分治疗组 10只、非治疗组 5只和正常对照组 2只 ,治疗组和非治疗组均将动物制作左后肢缺血模型 ,治疗组在模型制作后 14d,开始行患肢负压治疗 10 d(15 min·次 - 1 ) ;非治疗组不做负压治疗 ;正常对照组不行缺血模型制作及负压治疗 . 3组均行左后肢趾皮肤免疫组化染色 ,检测 SP免疫反应阳性纤维 .结果　非治疗组皮肤中 SP免疫反应阳性神经纤维均较正常对照组明显增多 (P<0 .0 1) ,治疗组较非治疗组减少(P<0 .0 1) ,但仍较正常对照组增多 .结论　肢体负压疗法可促进皮肤感觉神经纤维中 SP的释放     

针刺合针刀治疗周围性面神经麻痹疗效观察

目的　观察针刺合针刀治疗周围性面神经麻痹的疗效。方法　应用针刺合针刀治疗 4 0例 ,并与单纯针刺治疗 4 0例进行对照比较。结果与结论　治疗组和对照组总有效率无明显差异 ,但治疗组治愈率明显高于对照组。