The remedial effect of soluble interleukin-1 receptor type Ⅱ on endometriosis in the nude mouse model

Objective： Recent studies have shown that the local expression of soluble interleukin （IL） -1 receptor type Ⅱ （slL-1 R Ⅱ ） in endometrial tissue of women with endometriosis is decreased, and the depression of IL-1 R Ⅱ was more significant in infertile women than that in fertile women with endometriosis. In this research, we investigated the remedial effect of slL-1-R Ⅱ administration on endometriosis in the nude mouse model. Methods： Nineteen nude model mice with endometriosis were randomly divided into three groups： group A was treated by intraperitoneal administration with only slL-1 R Ⅱ for two weeks, group B was similarly treated with only IL- 1, and group C （control） was administered saline. After 2 weeks, the size of the ectopic endometrial lesions was calculated, and the expression of vascular endothelial growth factor （VEGF） and B-cell lymphoma leukemia-2 （Bcl- 2） were detected by immunohistochemistry. The IL-8 and VEGF levels in the peritoneal fluid （PF） and serum were also measured by enzyme-linked immunosorbent assay （ELISA）. Results： The mean size of ectopic endometrial lesion did not differ between the three groups （P 〉 0.05）. Compared with the control, the expression of VEGF and Bcl-2 was significantly lower in group A, and higher in group B. In the three groups, the levels of IL-8 in the PF and serum were highest in group A, and lowest in group B. Conclusion： slL-1 R Ⅱ may suppresse hyperplasia of ectopic endometriosis, perhaps by reducing the expression of certain cytokines, such as VEGF, IL-8, and Bcl-2, which could provide a new clinical strategy for the treatment of endometriosis.     

不同浓度利巴韦林对家兔外周血细胞的影响

目的探讨不同浓度利巴韦林对家兔外周血细胞的影响规律。方法将雄性家兔30只随机分为A、B、C、D、E5组，每组6只，分别注入利巴韦林5mg／kg、15mg／kg、25mg／kg、35mg／kg及注射生理盐水2ml，由家兔耳缘静脉缓慢推注，1次／d。7d为1个疗程，共2个疗程。分别在6个时间点观察家兔外周血RBC、Hb、网织红细胞（Ret）、WBC及Plt变化。结果RBC、Hb随着利巴韦林注射浓度增高而降低，而Ret明显上升，注射后第15天除A组外各组与E组比较差异均有统计学意义（均P〈0．05）；WBC除D组轻微降低（P〈0．05）外余各组无明显变化（均P〉0．05）；Plt随着利巴韦林注射浓度升高而明显增多，注射后第15天除A、B组外各组Plt与E组比较差异均有统计意义（均P〈0．05）。RBC、Hb、WBC及Plt在停药第15天后均能逐渐恢复至正常水平。结论利巴韦林可使家兔出现Plt增多和溶血，并随着利巴韦林浓度增高两者变化更为明显.     

融合功能磁共振影像的神经导航在脑皮质运动区肿瘤术中的应用

目的　探讨功能磁共振成像 (fMRI)的血液氧饱和水平检测 (BOLD)技术在脑皮质运动区肿瘤神经导航术中的应用价值。方法　将 5 8例脑肿瘤随机分组为试验组和对照组 ,试验组 30例 ,对照组 2 8例。肿瘤涉及第一运动区 (PMA) 18例 ;运动前区 (PM) 18例 ;第一运动感觉区 (PMSA) 11例 ;第一感觉区 (PSA) 9例 ;辅助运动区 (SMA) 2例。试验组运用手运动激发模式 ,行BOLD成像定位皮质运动区 ,并与MR的导航序列影像融合 ,应用于神经导航 ;对照组采用常规MR影像导航。结果　试验组肿瘤全切率为 86 7% ,对照组为 6 0 7% ,P <0 0 5。试验组术后患侧肢体肌力为 4 3度±1 1度 ,对照组为 2 5度± 1 9度 ,P <0 0 0 1。试验组术后致瘫率为 2 3 3% ,对照组为 71 4 %。试验组远期预后Karnofsky评分 (KPS)为 88± 2 7,对照组为 6 5± 32 ,P <0 0 1。结论　应用融合BOLD影像的神经导航术 ,可实现个体化、实时、精确地定位肿瘤及毗邻的脑皮质运动区 ,提高全切率 ,降低术后致瘫率。     

异佛尔酮二异氰酸酯免疫毒性的实验研究

本文选用小鼠溶血空斑形成试验研究了异佛尔酮二异氰酸酯(IPDI)对Bal/C小鼠脾细胞的作用,选用ANAE染色法观察了IPDI对SD大鼠外周血T细胞的影响,并采用体外培养方法研究了IPDI对人外周血T细胞在PHA刺激下增殖转化的影响。结果表明,IPDI对小鼠脾脏抗体生成细胞的数量和功能均有抑制作用,并呈现出剂量-反应关系;而对细胞免疫反应在大鼠和人均表现为双向作用。     

医联体框架下各省市关于强基层举措的现状分析

目的 国内各省市陆续开展医联体框架的建设来加强基层医疗卫生服务水平.了解不同地区的不同做法,为更好落实分级诊疗制度提出意见建议.方法 运用文献分析法,筛选有关医联体框架下强基层的文献,归纳后采用对比分析法,得出结论.结果 总结得出以病人、核心医院、合作医院与政府四类不同角度下医联体工作推行当中面临的问题.各省市就实际情况也开展了如双向转诊绿色通道、优质医疗资源下沉等强基层的措施.结论 目前全国医联体还处于探索模式,需要因地制宜的制度设计.通过医联体来加强基层医疗服务能力,从而达到基层首诊、双向转诊、上下联动、急慢分治的就医格局.     

运动负荷试验预测高血压的意义及运动触发高血压生化因子的变化

对４０例２２～３５岁血压正常的原发性高血压病患者子女以及２０例无高血压病者子女进行运动负荷试验。结果：前者１４例出现运动触发性高血压（≥３０．０／１２．７ｋＰａ），运动触发高血压者肾素活性、血管紧张素Ａ_２、血浆５－羟色胺浓度运动后均较正常血压者升高（Ｐ＜０．０５）；内源性洋地黄因子静息状态显著升高（Ｐ＜０．００１），而运动后降低；血小板５－羟色胺静息时无显著变化，运动后降低。高血压患者子女运动后心钠素较无高血压者子女升高（Ｐ＜０．０５），其中运动触发高血压者升高的较显著。前列环素、血栓素Ａ_２运动后较运动前升高，但差异无显著意义。     

肾盏憩室误诊为肾囊肿导致术后漏尿的防治探讨（附8例报道）

目的探讨肾盏憩室去顶术后漏尿的治疗方法及预防措施。方法选择2003年至2013年我院收治的8例术后漏尿的患者,术前均误诊为肾囊肿,术后证实为肾盏憩室。在逆行插入D-J管的基础上,1例漏尿自然愈合;7例行手术治疗,其中1例采取微创顺行切开扩大憩室颈的方法,4例行开放手术,2例行腹腔镜手术,术中缝合憩室颈开口,灼烧憩室腔黏膜。结果所有患者漏尿均愈合,患者无发热,无腰痛,B超显示患肾周围无积液,其中6例憩室完全消失,2例可见憩室较术前明显减小。结论肾盏憩室行去顶减压后出现的漏尿不易自行愈合,应采用手术治疗,预后较好。而术前正确诊断肾盏憩室是减少术后漏尿的关键。     

二例外籍艾滋病感染者监护案例的分析

通过对二例外籍艾滋病病毒感染者的发现及采取的措施,结合相关法规进行分析,显示在监测、感染者控制等方面存在相应的操作误区和不足。这些因素是可导致上报疫情延误,感染者失控,影响部门间协作机制的主要原因。提示外籍人员艾滋病监测控制政策存在漏洞,同时警示口岸一线人员熟悉掌握相关艾滋病卫生法规是做好外籍人员艾滋病监测和后续管理工作的基础。     

Microstructures and Soft Magnetic Properties of Fe73.5−xCu1Nb3Si13.5B9Gdx (x = 0–1.5) Alloys

In this experiment, the rare earth Gd element was added to Finemet alloy to observe the microstructure and soft magnetic properties. The experimental results showed that the samples with the addition of 0.5% Gd and 1.0% Gd can be quenched and cast normally, and the M_S of Fe₇₃Cu₁Nb₃Si_13.5B₉Gd_0.5 alloy was 10.41% higher than that of Finemet. After annealing, crystal grains of about 10 nm were formed. The μ_i and μ_m values of Fe₇₃Cu₁Nb₃Si_13.5B₉Gd_0.5 alloy were 25.51% and 22.23% higher, respectively, and the coercivity H_C was reduced by 12.19% compared to Finemet. At 1 kHz, the μ_e value of Fe₇₃Cu₁Nb₃Si_13.5B₉Gd_0.5 alloy at room temperature was 14.57% higher than that of Finemet, while the μ_e reached 162.34 k and 142.42 k at 90 °C and 150 °C (24% and 29.51% higher, respectively). The Fe_72.5Cu₁Nb₃Si_13.5B₉Gd_1.0 alloy had the best performance at 100 kHz, with higher μ_e values than Finemet across the ambient temperature range of 30 °C to 150 °C. After tension annealing, the μ_e values of Fe_72.5Cu₁Nb₃Si_13.5B₉Gd_1.0 alloy were 20–30% higher than those of Finemet.     

Identification of a novel ene reductase from Pichia angusta with potential application in (R)-levodione production

Asymmetric reduction of electronically activated alkenes by ene reductases (ERs) is an attractive approach for the production of enantiopure chiral products. Herein, a novel FMN-binding ene reductase (PaER) from Pichia angusta was heterologously expressed in Escherichia coli BL21(DE3), and the recombinant enzyme was characterized for its biocatalytic properties. PaER displayed optimal activity at 40 °C and pH 7.5, respectively. The purified enzyme was quite stable below 30 °C over a broad pH range of 5.0–10.0. PaER was identified to have a good ability to reduce the C C bond of various α,β-unsaturated compounds in the presence of NADPH. In addition, PaER exhibited a high reduction rate (k_cat = 3.57 s⁻¹) and an excellent stereoselectivity (>99%) for ketoisophorone. Engineered E. coli cells harboring PaER and glucose dehydrogenase (for cofactor regeneration) were employed as biocatalysts for the asymmetric reduction of ketoisophorone. As a result, up to 1000 mM ketoisophorone was completely and enantioselectively converted to (R)-levodione with a >99% ee value in a space–time yield of 460.7 g L⁻¹ d⁻¹. This study provides a great potential biocatalyst for practical synthesis of (R)-levodione.

Asymmetric reduction of electronically activated alkenes by ene reductases (ERs) is an attractive approach for the production of enantiopure chiral products.