A Woman Needs to Know She Is a BRCA Carrier Before She Develops Breast Cancer

Annals of Surgical Oncology -     

MIF promoter polymorphism increases peripheral blood expression levels,contributing to increased susceptibility and poor prognosis in hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is one of the most common malignancies in the world. The etiology and pathogenesis of HCC remain unclear. Macrophage migration inhibitory factor (MIF) plays a critical role in the pathogenesis of hepatocellular carcinoma. The association between MIF polymorphisms and its expression level in HCC has rarely been demonstrated. In the present study, the peripheral blood of 202 patients with HCC (HCC group), 242 patients with chronic hepatitis B (CHB group), 215 patients with liver cirrhosis (LC group) and 227 healthy volunteers (normal group) were collected, DNA was extracted and the target fragment of MIF gene was amplified using PCR. The products were then sequenced, and the expression levels of MIF protein were tested using ELISA. The results showed that the MIF rs755622 polymorphism was associated with an increased susceptibility and metastasis of HCC, and that the genotypes GC and CC were associated with poor prognosis of HCC. Compared with the normal, CHB and LC groups, the expression of MIF in the peripheral blood of the HCC group was significantly increased, and the high expression was associated with to poor prognosis. In the HCC group, MIF protein levels for genotypes GC and CC were increased compared with those of genotype GG. The current study indicated that the MIF rs755622 polymorphism is associated with susceptibility and metastasis of HCC, and that the GC and CC genotypes may be indicators of poor prognosis, which may be ascribed to the MIF rs755622 polymorphism leading to elevated MIF protein expression in peripheral blood.     

Outcomes of Stage I and II Breast Cancer with Nodal Micrometastases Treated with Mastectomy without Axillary Therapy

Breast Cancer Research and Treatment - Studies that report equivalent oncologic outcomes of sentinel lymph node biopsy (SLNB) alone versus axillary lymph node dissection (ALND) for T1-2N1mi breast...     

Predictive Value of p62 Protein in the Recurrence of Oral Squamous Cell Carcinoma

Background: Tumor recurrence is one of the major problems that affect the postoperative efficacy of oral squamous cell carcinoma (OSCC) treatment. Autophagy is known to have a dual-sided effect on tumors. However, studies on autophagy and prognosis are limited. The purpose of this study is to analyze the relationship between autophagy and OSCC recurrence. Materials and methods: 72 patients with OSCC were followed for 5 years after curative surgery. Tissue specimens from each patient were divided into tumor, normal, and marginal groups. Autophagy protein expression was assessed by western blotting and immunofluorescence. Statistical significance was evaluated using the chi-square test. The Kaplan-Meier method was employed to construct a survival curve, and the log-rank test was used to compare survival rates. Cox regression was utilized to describe independent prognostic risk factors. ROC analysis was performed to evaluate the utility of p62 and LC3II expression in assessing OSCC recurrence. Results: Correlations were observed between survival rates and T stage (p = 0.00439), lymph node metastasis (p < 0.001), tumor differentiation (p = 0.00423), and changes in p62 expression (p < 0.001). Multivariate analysis showed lower p62 protein expression in the tumor group compared to the normal group with a higher risk of recurrence (RR = 3.713), and lower p62 protein expression was found in the marginal group compared to the tumor group, with a lower risk of recurrence (RR = 0.170). Conclusion: Expression of p62 protein is one of the important factors influencing relapse in OSCC patients and can be used as an early predictor of OSCC relapse.     

Nutlin-3-induced redistribution of chromatin-bound IFI16 in human hepatocellular carcinoma cells in vitro is associated with p53 activation

Aim:

Interferon-γ inducible protein 16 (IFI16), a DNA sensor for DNA double-strand break (DSB), is expressed in most human hepatocellular carcinoma cell (HCC) lines. In this study we investigated the re-localization of chromatin-bound IFI16 by Nutlin-3, a DNA damage agent, in HCC cells in vitro, and the potential mechanisms.

Methods:

Human HCC SMMC-7721 (wild-type TP53), Huh-7 (mutant TP53), Hep3B (null TP53) and normal fetal liver L02 cell lines were examined. DSB damage in HCC cells was detected via γH2AX expression and foci formation assay. The expression of IFI16 and IFNB mRNA was measured using RT-PCR, and subcellular localization and expression of the IFI16 protein were detected using chromatin fractionation, Western blot analysis, and fluorescence microscopy.

Results:

Treatment of SMMC-7721 cells with Nutlin-3 (10 μmol/L) or etoposide (40 μmol/L) induced significant DSB damage. In SMMC-7721 cells, Nutlin-3 significantly increased the expression levels of IFI16 and IFNB mRNA, and partially redistributed chromatin-bound IFI16 protein to the cytoplasm. These effects were blocked by pretreatment with pifithrin-α, a p53 inhibitor. Furthermore, Nutlin-3 did not induce ectopic expression of IFI16 protein in Huh-7 and Hep3B cells. Moreover, the association of IFI16 with chromatin and Nutlin-3-induced changes in localization were not detected in L02 cells.

Conclusion:

Nutlin-3 regulates the subcellular localization of IFI16 in HCC cells in vitro in a p53-dependent manner.     

西部高原地区基层中西医结合医疗卫生情况调查分析

目的:通过调查分析青海省西宁市大通县地区基层中西医结合医疗卫生实施情况,以期对西部高原多民族聚集地区的基层中西医结合医疗卫生发展有一定的指导作用。方法:根据现有文献资料,结合当地实际情况设计调查问卷,对青海大通地区居民进行基层中西医结合医疗情况问卷调查,统计分析数据。结果:目标人群对基层中西医结合医疗需要迫切,但需求未得到充分满足。结论:针对以青海大通县为代表的西部高原多民族聚集地区居民基层中西医结合医疗保障亟需加强的情况,应积极完善提高当地卫生医疗水平,推广中西医结合优势治疗,加大中医药治疗的力度等,以满足基层群众对高质量的中西医结合医疗卫生服务的迫切需求。     

p53 alpha-Helix mimetics antagonize p53/MDM2 interaction and activate p53

Overexpression or hyperactivation of MDM2 contributes to functional inactivation of wild-type p53 in nearly 50% of tumors. Inhibition of p53 by MDM2 depends on binding between an NH(2)-terminal (residues 16-28) p53 alpha-helical peptide and a hydrophobic pocket on MDM2, presenting an attractive target for development of inhibitors against tumors expressing wild-type p53. Here we report that novel p53 alpha-helical peptide mimics based on a terphenyl scaffold can inhibit MDM2-p53 binding in vitro and activate p53 in vivo. Several active compounds have been identified that inhibit MDM2-p53 binding in an ELISA assay with IC(50) of 10 to 20 micromol/L and induce p53 accumulation and activation in cell culture at 15 to 40 micromol/L. These results suggest that p53 alpha-helical mimetics based on the terphenyl scaffold may be developed into potent p53 activators.     

开展产学研合作教育培养应用型护理人才

产学研合作教育培养应用型人才,是高职高专教育在办学中努力去探索和实践的长期课程。通过全面提高对产学研合作教育观念、内涵的认识,开展产学研合作教育,培养高素质应用型人才,加强实训基地建设,改善实践教学的平台环境,在努力拓宽产学研合作教育的思路等方面进行了探索。经过系统的研究与教学实践,取得了满意的效果。