藏药大花龙胆正丁醇部位的化学成分研究

目的研究藏药大花龙胆Gentiana szechenyii花枝乙醇提取物的正丁醇部位的化学成分。方法通过制备高效液相色谱法分离纯化,利用各种谱学技术鉴定化合物的结构。结果从大花龙胆花枝乙醇提取物的正丁醇部位分离得到6个化合物,分别鉴定为大花龙胆酯苷A(1)、大花龙胆苷A(2)、异金雀花素-2″-O-吡喃葡萄糖苷(3)、异金雀花素(4)、乌奴龙胆苷D(5)和平龙胆酯苷(6)。结论化合物1为1个新的环烯醚萜苷类化合物,命名为大花龙胆酯苷A。     

前交叉韧带断裂和重建的临床流行病学分析

BACKGROUND: Anterior cruciate ligament (ACL) reconstruction is the primary treatment method for ACL rupture. Currently, studies on ACL reconstruction involve histology and embryology, anatomical structure, biomechanics, reconstruction materials, operating technology, and rehabilitation after reconstruction. However, clinical epidemiological studies describing ACL rupture and reconstruction remain scarce. OBJECTIVE: To analyze the clinical epidemiological characteristics of ACL rupture and reconstruction to provide guidance for prevention and treatment of ACL rupture. METHODS: Data of 352 patients for age, gender, cause and mechanism of injury, treatment time, and the impact of ACL rupture on menisci and articular cartilage were gathered. Meanwhile, the events during surgery, operation methods and reconstruction materials were analyzed. RESULTS AND CONCLUSION: ACL rupture mostly occurred in young men, and happened more often to the left knee; male patients got hurt in basketball, football and accidental injuries, while female patients got hurt in the accidental injuries, badminton and skiing injuries, internal rotation with valgus stress accounted for the predominant injury mechanism. The ACL reconstruction was mostly performed within 1-3 months after ACL rupture, often accompanied by meniscal and articular cartilage damage. Lateral meniscus injury incidence was relatively stable, medial meniscus injury incidence increased significantly over the half year after ACL rupture. Most articular cartilage injury occurred to patellar cartilage. A significant increase in medial condylar cartilage damage over 1 year after ACL rupture was often observed. Anatomic single-bundle ACL reconstruction was the primary surgical approach, the resident ridge and the lateral bifurcate ridge could be used to position bone tunnel and autogenous semitendinosus and gracilis tendon were the most commonly used reconstruction materials. Our results indicate that anatomic ACL reconstruction should be performed as early as possible in restore knee joint stability and prevent secondary injury of the medial meniscus and cartilage of medial femoral condyle. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

新型可塑形同种骨修复材料修复运动性关节软骨损伤

BACKGROUND: Currently, the components and preparation methods of plastic bone repair materials remain controversial, and rare studies focus on their repair effects on sport-related articular cartilage injury. OBJECTIVE: To analyze the effect of a novel plastic homogeneous bone repair material to repair sport-related articular cartilage injury. METHODS: Thirty-six New Zealand rabbits were selected to prepare articular cartilage injury models, and were randomized into two groups. Model rabbits were repaired with the novel plastic homogeneous bone material (a composite of demineralized bone matrix and collagen) as experimental group, while the others repaired with sodium hyaluronate gel as control group. At 3 weeks after repair, treadmill system was utilized to stimulate sports training after athlete injury, for five consecutive sessions of 30 minutes each. At 16, 18 and 20 weeks after repair, the defect region was observed histologically. RESULTS AND CONCLUSION: At 16 weeks after repair, the defect region healed well and integrated with the surrounding tissues, and no significant inflammatory reaction appeared around the material in the experimental group; in the control group, a fissure appeared at the defect region but with no inflammatory reaction, and the material integrated with the interstitial site. At 18 weeks after repair, closely arranged acellular bone matrix could be found in the defect region, fibrous connective tissues were fewer, and there was a new bone formation around the edge of host bone in the experimental group; the defect region healed, but new bones were fewer in the control group. At 20 weeks after repair, the defect region in the experimental group was filled with fibrous connective tissues, in which there were numerous new blood vessels, and a few of new tissues appeared around the edge of host bone; the defect region in the control group was improved and no neovascularization occurred. These findings suggest that the novel plastic homogeneous bone material can promote the repair of sport-related articular cartilage injury with less rejection reactions.     

MRI-三维重建动态测量颅脑创伤模型大鼠的空腔变化

BACKGROUND: Currently, morphological observations of brain cavity after traumatic brain injury (TBI) via cadavers or animal specimen are difficult to obtain dynamic changes.     

超微超顺磁性纳米氧化铁标记犬口腔黏膜上皮细胞与磁共振成像实验

BACKGROUND: Epithelial cells are commonly used as the seed cell in tissue engineering; however, there is still a lack of an effective in vivo noninvasive trace technology. OBJECTIVE: To investigate the feasibility of labeling canine oral epithelial cells with ultrasmall superparamagnetie iron oxide (USPIO) and magnetic resonance imaging (MRI) in vitro.  METHODS: Oral epithelial cells from beagles were primary cultured, and then labeled by 0.75 mg/L poly-L-lysine combined with USPIO (0, 5, 10, 25, 50 and 100 mg/L), respectively. To determine the optimal dosage, the intracellular iron expression was identified by Prussian blue staining, and the cell viability in different groups was detected by cell counting kit-8. Finally, 2×105 labeled cells were suspended with 1 mL PBS buffer, and were screened using 3.0 T MR on T2*WI sequences in vitro.  RESULTS AND CONCLUSION: USPIO prepared with 0.75 mg/L poly-L-lysine could successfully label dog oral epithelial cells. Prussian blue staining showed intracellular blue spots, and the intracellular blue spots became more with the concentration increasing and saturated at the concentration of 25 mg/L. Cell counting kit-8 indicated that the cell viability did not change when the concentration < 25 mg/L. Among the T2*WI sequences, the MRI signal intensity decreased with the concentration increasing. In conclusion, canine oral epithelial cells can be effectively labeled with USPIO making no impact on cell viability when the concentration < 25 mg/L, and MRI can be used to track these labeled cells in vitro．     

乳腺癌患者术后淋巴水肿与其相关症状的关系研究

目的 调查乳腺癌患者术后不同程度淋巴水肿发生状况及其相关症状情况,分析两者之间的关系。 方法 采用便利抽样及目的抽样方法,选择乳腺癌行腋窝淋巴结清扫术后患者 224 例, 采用肢体周径测量法判断淋巴水肿严重程度, 采用乳腺癌淋巴水肿症状体验指数（ Breast Cancer and Lymphedema Symptom Experience Index , BCLE鄄SEI ）评估患者淋巴水肿相关症状体验。 结果 224 例患者中,无水肿 94 例（ 42.0% ）,轻度水肿 46 例（ 20.5% ）,重度水肿 84 例（ 37.5% ）。 轻度淋巴水肿患者的症状个数、症状严重程度及症状困扰总分高于无淋巴水肿患者（ P=0.007 ）;重度淋巴水肿患者的症状个数、症状严重程度及症状困扰总分高于轻度淋巴水肿患者（ P=0.017 ）。 轻度水肿患者的上肢肿胀、患肢沉重、患肢疲乏、患肢紧绷、患肢僵硬、手臂受限、 肘部受限、 手腕受限的发生率高于无水肿患者。 结论 淋巴水肿早期就出现相应症状,且症状体验随着水肿加重而越发严重。 关注患肢肿胀、患肢沉重等淋巴液积聚症状,可早 期发现淋巴水肿并给予早期干预,阻止或延缓水肿程度的进展。     

Two novel phorbol esters from Croton tiglium L.

To investigate the phytochemical constituents of aboveground parts of Croton tiglium L. (family Euphorbiaceae), its ingredients were isolated by repeated chromatography on silica gel, Sephadex LH-20, ODS and preparative HPLC. Their structures were identified based on 1D, 2D NMR and mass spectral analysis. A total of 10 phorbol esters were obtained. Among them, compound 1 (12-O-(2-methyl)butyryl-4α-deoxyphorbol-13-isobutyrate) and compound 10 (20-formyl-4α-deoxyphorbol-13-acetate) were two new compounds, and the other eight were known compounds.     

肺泡样肺腺癌中端锚聚合酶的表达及其与WNT 信号通路的关系

摘要： 目的 探讨肺泡样肺腺癌中端锚聚合酶（TNKS）的表达情况及其与 WNT 信号通路的关系。方法 收集 72 例单一亚型肺泡样肺腺癌组织（肺腺癌组）和 67 例癌旁正常肺组织（癌旁组）标本, 应用免疫组化法检测 2 组 TNKS、 β-连环蛋白 （β-catenin） 和 c-myc 蛋白的表达情况, 并分析 3 种蛋白在肺腺癌组织中表达的相关性。Western blot 检测 TNKS 在肺腺癌组和癌旁组中表达的差异性。结果 TNKS 蛋白主要于细胞质表达; β-catenin 蛋白在肺腺癌组主要于细胞质和细胞核表达, β-catenin 在癌旁组主要于细胞质表达, 少量于细胞核表达; c-myc 蛋白主要于细胞核表达。TNKS、 β-catenin 和 c-myc 蛋白在肺腺癌组中的阳性表达率均高于癌旁组 （P＜0.05）。β-catenin 蛋白细胞质和细胞核中的表达与 TNKS 及 c-myc 的表达水平均呈正相关（均 P＜0.05）。Western blot 检测示 TNKS 在肺腺癌组中的相对表达水平高于癌旁组 （0.497±0.021 vs. 0.237±0.015,t=13.00, P < 0.01）。结论 TNKS 在肺腺癌中异常高表达, 可能是通过调控 WNT 信号通路, 从而促进肺腺癌的发生, 抑制 TNKS 表达或可成为治疗肺腺癌的新靶点。