IL－2基因转移的瘤苗与IL－1、低剂量环磷酰胺合用后的肿瘤治疗作用及其免疫学机理

经ＩＬ－２基因转移瘤苗治疗后荷瘤小鼠肺转移结节明显减少、存活期明显延长，与ＩＬ－１或低剂量Ｃｙ合用后，可使荷瘤小鼠的肺转移结节更少，存活期更长，特别是当ＩＬ－２基因转移的瘤苗、ＩＬ－１、低剂量Ｃｙ三者合用时抗肿瘤转移效果最强。对瘤苗治疗后荷瘤小鼠体内免疫功能的研究表明，小鼠脾脏ＣＴＬ活性、ＮＫ活性、ＩＬ－２导的ＬＡＫ活性均显著增强，脾脏细胞分泌ＩＬ－２和ＴＮＦ水平也显著升高，当与ＩＬ－１、低剂量Ｃｙ合用时，上述抗肿瘤免疫功能升高得更加明显，可见ＩＬ－２基因转移的瘤苗能通过有效地激活体内抗肿瘤免疫功能而具有显著的抗肿瘤转移效果，将其与ＩＬ－１或低剂量Ｃｙ联合应用时抗肿瘤效果更佳，当三者同时合用时抗肿瘤效果最佳。     

宫腔镜下输卵管双层插管治疗输卵管梗阻108例效果分析

目的 :为进一步提高宫腔镜下插管治疗输卵管严重梗阻的疏通率及术后妊娠率。对象和方法 :本文对 1 0 8例患有输卵管完全或部分梗阻导致不孕的妇女 ,在宫腔镜窥视下经第一次插管注液不能疏通者 ,进行双层插管疏通治疗。结果 :经第一次插管注液完全疏通的输卵管由术前的 3.30 %上升到 33.0 2 % (P<0 .0 5) ,在此基础上进行双层插管 ,完全疏通率 33.0 2 %上升到 57.59% (P<0 .0 5) ,经第二次宫腔镜检查 ,完全疏通率略有下降 ,但 P>0 .0 5。术后一年随访 ,怀孕 33例 (30 .56 % )。结论 :宫腔镜下输卵管双层插管对输卵管严重梗阻有较好疗效 ,可提高术后怀孕率     

Relation between responsiveness to neurotransmitters and complexity of epileptiform activity in rat hippocampal CA1 neurons

PURPOSE: Our previous works suggested that sensitivity of neurons with chaotic firing patterns to stimuli is significantly greater than that in neurons with periodic firing patterns, which shows that responsiveness of neurons may depend on the complexity of the firing series. This study was performed to determine the relation between responsiveness of the hippocampal CA1 neurons with epileptiform activity (EA) to neurotransmitters and their complexity of firing series. METHODS: Firing series of CA1 neurons were recorded extracellularly in rat hippocampal slice. Approximate entropy was used to describe the complexity of the interspike interval (ISI) series. EA was induced by local application of penicillin (1,000 IU/ml). The change of firing rate induced by neurotransmitters (glutamate and gamma-aminobutyric acid) was compared with that of the degree of complexity of ISI series in the process of EA. RESULTS: The excitatory responses to glutamate and the inhibitory responses to gamma-aminobutyric acid in CA1 neurons appeared to be decreased during the process of penicillin-induced EA. However, during this same process, the approximate entropy of the ISI series also was decreased significantly. CONCLUSIONS: The results suggest that the reduced responses to neurotransmitters of the CA1 neurons appear to be closely related to the onset of EA. Furthermore, these neurons show that the changes in responsiveness are closely parallel to the decrease of degree of complexity of firing series during penicillin epileptogenesis.     

Aplasia cutis congenita of the trunk with fetus papyraceus

Aplasia cutis congenita (ACC) is the absence of localized or widespread areas of skin at birth. A frequently cited classification schema is based on location and the presence of associated anomalies. Histologically it is characterized by dermal fibrosis and absence of adenexal structures. We present a newborn female with extensive truncal ACC associated with fetus papyraceus.     

CART in the dorsal vagal complex: sources of immunoreactivity and effects on Fos expression and food intake

CART-peptide (CARTp) has been shown to suppress food intake, particularly when injected into the 4th ventricle of rats, and the presence of CART in nodose ganglia suggested a role in satiation. Based on retrograde tracing from the DVC combined with CART immunohistochemistry and supranodose vagotomy, we found that CART immunoreactivity in varicose fibers of the dorsal vagal complex originates from vagal afferents, sparse projections from the medullary reticular formation and the arcuate/retrochiasmatic nucleus of the hypothalamus, and most likely also from local CART neurons in the area postrema and NTS. In the nodose ganglia, 17% of neurons with projections to the stomach and 41% to the duodenum express CART-IR. CART-IR vagal afferents significantly contribute to the rich fiber plexus in mainly the commissural NTS and the adjacent area postrema. Injections of CARTp into the 4th ventricle strongly suppressed sucrose drinking and stimulated expression of c-Fos in the NTS. Injections of CARTp directly into various subnuclei of the NTS were less effective in suppressing food intake. The findings suggest that the critical site for CART's suppression of food intake is not in the termination zone of CART-containing vagal afferents in the commissural NTS, and that CART release from vagal afferent terminals plays a minor role in satiation. The functional role of CART in vagal afferents and the site of food intake suppression by 4th ventricular CARTp remain to be determined.     

Effects ofcis-9,trans-11-conjugated linoleic acid on cancer cell cycle

Objectives To determine the effect of cis-9, trans-11-conjugated linoleic acid on the cell cycle of mammary cancer cells (MCF-7) and its possible mechanism of inhibition cancer growth. Methods Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A, B₁, D₁, p16^ink4a and p21^cip/wafl of MCF-7 cells which were treated with various c9, t11-CLA concentrations (25 mM, 50 mM, 100 mM and 200 mM) of c9, t11-CLA for 24 and 48 h, with negative controls (0.1% ethanol). Results The cell growth and DNA synthesis of MCF-7 cells were inhibited by c9, t11-CLA. MCF-7 cells, after treatment with various c9, t11-CLA doses mentioned above for 8 days, the inhibition frequency was 27.18%, 35.43%, 91.05%, and 92.86%, respectively and the inhibitory effect of c9, t11-CLA on DNA synthesis (except for 25 mM, 24 h) incorporated significantly less³H-TdR than did the negative control (P<0.05 andP<0.01). To further investigate the influence on the cell cycle progression, we found that c9, t11-CLA may arrest the cell cycle of MCF-7 cells. Immunocytochemical staining demonstrated that MCF-7 cells preincubated in media supplemented with different c9, t11-CLA concentrations at various times significantly decreased the expressions of PCNA, and Cyclin, A, B₁, D₁ compared with the negative controls (P<0.01), whereas the expressions of p16^ink4a and p21^cip/wafl, cyclin-dependent kinases inhibitors (CDKI), were increased. Conclusions The cell growth and proliferation of MCF-7 cells is inhibited by c9, t11-CLA by blocking the cell cycle, which reduces expressions of cyclin A, B₁, D₁ and enhances expressions of CDKI (p16^ink4a and p21^cip/wafl).     

F6H8对兔角膜内皮细胞影响的研究

目的 研究F6H8对角膜内皮细胞的影响。方法 成年新西兰白兔15只,实验组12只,对照组3只,均行前房穿刺术,注入F6H8（实验组）或BSS（对照组）0．15ml,手术前后定期行裂隙灯、角膜内皮摄像及组织学检查。结果 术后4周,实验组与F6H8相接触的下方角膜内皮细胞形态不规则,角膜内皮细胞数目下降,与术前比较差异有显著意义（F＝16．602,P＝0．002）。光镜下见上皮细胞空泡变性,术后4周有角膜后膜形成。透透电镜下见线粒体,内质网肿胀及细胞核变性。结论 F6H8作为一种硅油清洗剂使用时应避免进入前房,目前尚不能作为眼内长期填充物。     

Heterotrimeric GTP-binding proteins in the lacrimal acinar cell endomembrane system

Secretagogues accelerate traffic in the lysosomal and basal-lateral pathways, as well as in the regulated apical secretory pathway, of lacrimal acinar cells. It has been proposed that alterations of protein segregation in compartments where these traffic pathways intersect may influence autoimmune responses. Heterotrimeric GTP-binding proteins couple secretagogue receptor ligand binding to activation of intracellular signaling cascades, but they are also suggested to participate in endomembrane traffic phenomena. Distributions of G(o), G(i3), G(q), G(11), and two G(s)isoforms were mapped in reconstituted lacrimal acini by confocal immunofluorescence microscopy and in lysates of the reconstituted acini by analytical subcellular fractionation. All G proteins examined were detected at low levels in isolated compartments (blm(i,j)) believed to represent the basal-lateral plasma membrane. G(i3), G(11), and the G(s)isoforms were concentrated in a series of isolated compartments believed to be related to domains of a basal-lateral endosome with sorting and recycling functions (ble-s/r(i,j,k)), a distinct endosomal compartment with basal-lateral membrane-like composition (e-blml), and domains of the trans-Golgi network believed to be involved in traffic to and from the basal-lateral membrane (tgn-blmr). G(o)and G(q)were concentrated in compartments believed to represent a mixture of immature and mature secretory vesicle membranes (isvm and svm) and domains of the trans-Golgi network compartment believed to mediate traffic to secretory vesicles (tgn-svr) and to pre-lysosomes (tgn-lr). Confocal fluorescence microscopy confirmed the presence of both basal-lateral membrane and intracellular pools of the G proteins. Stimulation with 10 microM carbachol for 20min caused a component of the G(o)to redistribute away from the isvm+svm; components of the G(i3), G(q), and G(s)to redistribute away from the tgn-svr+tgn-lr; and a component of the G(i3)to redistribute away from the ble-blml+tgn-blmr. Thus, these proteins may participate in endomembrane traffic steps activated by cholinergic stimulation in addition to playing their classical roles in plasma membrane signal transduction.     

醋酸棉酚对大鼠成熟卵泡的颗粒细胞产生雌二醇及孕酮功能影响的离体观察

给30天幼龄大鼠皮下注射12IU 孕马血清,48小时后采集发育成熟的卵泡颗粒细胞进行离体培养,并在培养基中加入不同浓度的醋酸棉酚,以观察醋酸棉酚对卵巢甾体激素合成功能的影响。结果发现,棉酚对颗粒细胞分泌孕酮的能力以及对hCG 促进颗粒细胞生成孕酮的能力均有抑制作用。这种抑制作用随棉酚剂量的增加及作用时间的延长而增强。但同样剂量的棉酚对颗粒细胞利用睾酮转化为雌二醇的能力无明显抑制作用。这提示棉酚对颗粒细胞内孕酮生物合成系统有直接抑制作用,而对芳香化酶的活性似无明显干扰作用,表明棉酚对卵巢内甾体激素合成功能的抑制具有选择性。此外,本实验还发现,hCG 的作用在未被完全阻断时,能不同程度地削弱棉酚对颗粒细胞分泌孕酮功能的抑制作用。     

强啡肽A1-13在脑源性神经营养因子治疗新生大鼠缺氧缺血性脑损伤中的变化

目的 探讨强啡肽A1-13在脑内移植脑源性神经营养因子（BDNF）载体细胞治疗新生大鼠缺氧缺血性脑损伤（HIBI）中的作用。方法 7日龄新生大鼠随机分为HIBI BDNF组（A）,HIBI BDNF U50,488H组（B）,单纯HIBI组（C）和假手术组（D）,左侧颈总动脉结扎联合低氧吸入形成新生大鼠HIBI,伤后立即脑内植入BDNF载体细胞（A,B）或空白细胞（C）,植入后延髓池内注射阿片к受体激动剂U50,488H（0.5μg,B）,观察处理后0,1,3d左右侧皮层和海马强啡肽A1-13免疫活性物质（ir-DynA1-13)含量的变化及处理后1d脑含水量,丙二醛（MDA）含量和细胞凋亡情况。结果 左侧皮层ir-DynA1-13含量处理后不同时间A,B或C组显著高于D组,1,3dA或B组显著低于C组;左侧海马给药后当天（A,B,C）,1d(B,C),3d(C)显著高于D组,给药（1d(A),3d(B)显著低于C组,新生大鼠HIBI后1d,左脑含水量,MDA和细胞凋亡百分率,A,B或C组显著高于D组,A组显著低于C组。结论 强啡肽A1-13参与HIBI病理生理过程,植入BDNF载体细胞减轻HIBI的作用途径之一。是抑制了强啡肽A1-13与特异性阿片к受体相结合所产生的加重BIHI的效应。