肝癌组织中p27蛋白表达与肿瘤增殖凋亡活性的研究

研究肝细胞癌组织中p27表达对细胞增殖与凋亡活性的影响.采用Elivision法检测47例肝癌及相应癌旁肝组织中p27及PCNA的表达,TUNEL法检测原位细胞凋亡.结果显示肝癌组织中细胞增殖与凋亡指数明显高于癌旁肝组织.肝癌组织中p27染色指数明显低于癌旁肝组织与正常肝组织.低分化肝癌组织中p27表达和凋亡指数均明显降低.存在肝外转移的肝癌组织中p27表达降低而增殖指数明显增高.进一步研究显示,p27高表达组肝癌组织中凋亡指数明显高于低表达组,而增殖指数却显著降低.提示p27蛋白表达降低与肝癌的发生和进展有着密切关系,并可能是导致肝癌细胞增殖凋亡失衡的因素之一.     

基于医务人员视角的远程医疗服务使用意愿和关键问题研究

目的:分析我国医务人员远程医疗服务使用意愿和关键问题,以完善远程医疗服务体系。方法:2019年10—11月,对福建、海南、河南、湖南、贵州、四川和青海7个省份中已参与过远程医疗的邀请方和受邀方医务人员进行电子问卷调查,主要内容为医务人员对远程医疗的使用意愿、满意度情况和认知评价情况等。通过描述性统计分析和非参数检验,探...     

多系统萎缩的临床特征与疾病进展的特点

目的探讨多系统萎缩(MSA)的临床特征与疾病进展的特点。方法回顾性分析28例拟诊MSA患者的临床资料;用代表日常生活活动能力(ADL)的3个重要事件(辅助行走、依靠轮椅、卧床状态)对疾病进展进行评估;通过Kaplan-Meier曲线分析各亚组间的差异。结果3个以上系统受累26例(92.9%),普遍存在自主神经功能障碍。从起病进展为临床MSA平均2年;进展为辅助行走、依靠轮椅和卧床状态分别为3年、5年、7年。运动和自主神经系统同时受累的患者起病3年内疾病进展的危险性明显增加(P<0.01)。结论MSA是一种多系统受累的慢性进展性疾病,从起始症状到同时出现运动和自主神经功能障碍的间隔时间能够预测MSA功能衰退的进展。     

边防海岛战士心理健康状况及人格特征与应对方式的研究

目的了解边防海岛战士的心理健康状况,人格特征及应对方式的关系。方法采用症状自评量表(SCL-90)、艾森克个性问卷(EPQ)和简易应对方式问卷对960名边防海岛战士进行心理测试,了解他们心理健康状况,人格特征和应对方式特点及其影响因素。结果(1)边防海岛战士SCL-90各因子分均显著高于军人常模;(2)边防海岛战士应对方式与心理健康状况密切相关,积极应对方式与SCL-90某些因子相关,消极应对方式与SCL-90各因子均呈显著正相关(P<0.01);(3)边防海岛战士在面对应激时所采取的应对方式与人格特征密切相关,消极应对方式与人格问卷中的P分(精神质)和N分(神经质)有显著或高度显著正相关(P<0.01),积极应对方式与人格问卷中的P分(精神质)呈负相关,和E分(内外向)呈正相关。结论边防海岛战士心理健康状况差于军人总体水平,人格特征及应对方式对心理健康水平有较大影响。     

应对方式的干预对边防海岛军人心理健康的影响

目的通过改变边防海岛军人的应对方式来提高他们的心理健康水平,提高部队的战斗力。方法运用多种形式组合的综合心理干预对边防海岛军人的应对方式进行干预,选用症状自评量表(SCL-90)、简易应对方式量表对边防海岛军人行心理测量。结果采取综合心理干预的边防海岛军人的症状自评量表(SCL-90)各项因子分均低于未采取综合心理干预的边防海岛军人(P<0.01),与未采取综合心理干预的边防海岛军人相比较,采取综合心理干预的边防海岛军人更多地采取积极的应对方式(P<0.01)。结论通过综合心理干预,让边防海岛军人更多地采取积极的应对方式对待生活和对抗应激,对提高和维护边防海岛军人的心理健康水平,确保部队的战斗力具有积极的意义。     

疏肝健脾法对肝癌肝动脉化疗栓塞后免疫功能的影响

目的探讨疏肝健脾法对肝癌患者肝动脉化疗栓塞后免疫功能的影响。方法103例中晚期原发性肝癌患者随机分为治疗组(n=52)及对照组(n=51)。两组患者介入治疗后均予西药常规护肝治疗及对症处理,治疗组在介入治疗前7 d开始服用疏肝健脾中药,介入治疗后随症加减,对照组单纯行肝动脉化疗栓塞。两组患者分别在第1次介入前、第2次介入后4周检查T淋巴细胞亚群。结果第1次介入前治疗组和对照组的NK细胞、CD3、CD4、CD4/CD8比值差异无显著性,P均>0.05。第2次介入后4周对照组的NK细胞、CD3、CD4、CD4/CD8比值均下降;而治疗组的NK细胞、CD3、CD4、CD4/CD8比值均升高,与治疗前比较有统计学意义,P均<0.05。结论疏肝健脾法可提高肝癌患者TACE后的免疫功能。     

Type 2 Diabetes,Fasting Glucose,Hemoglobin A1c Levels and Risk of Primary Open-Angle Glaucoma: A Mendelian Randomization Study

PurposeTo evaluate the potential causal associations between type 2 diabetes and fasting glucose and HbA1c levels and the risk of primary open-angle glaucoma (POAG) in European and East Asian populations.MethodsWe selected genetic variants (P < 5 × 10⁻⁸) for type 2 diabetes (898,130 Europeans; 433,540 East Asians), fasting glucose, and HbA1c (196,991 Europeans; 36,584 East Asians) from three meta-analyses of genome-wide association studies (GWAS). The GWAS for POAG provided summary statistics (192,702 Europeans; 46,523 East Asians). Mendelian randomization (MR) analysis was accomplished using the inverse variance–weighted method, weighted-median method, MR Egger method, and MR-Pleiotropy RESidual Sum and Outlier test.ResultsGenetically predicted type 2 diabetes was potentially positively associated with POAG in the European ancestry (body mass index [BMI]–unadjusted: odds ratio [OR] = 1.07, 95% confidence interval [CI], 1.01–1.14, P = 0.028; BMI-adjusted: OR = 1.07, 95% CI, 1.01–1.15, P = 0.035), but not in the East Asian ancestry (BMI-unadjusted: OR = 1.01, 95% CI, 0.95–1.06, P = 0.866; BMI-adjusted: OR = 1.00, 95% CI, 0.94–1.05, P = 0.882). There was no evidence to support a causal association of fasting glucose (European: OR = 1.19, P = 0.157; East Asian: OR = 0.94, P = 0.715) and HbA1c (European: OR = 1.27, P = 0.178; East Asian: OR = 0.85, P = 0.508) levels with POAG.ConclusionsThe causal effect of type 2 diabetes on the risk of POAG is different in European and East Asian populations. The point estimates of fasting glucose and Hb1Ac with POAG are large but not statistically significant, which prompts the question of statistical power.     

Correction of RNA splicing defect in β654-thalassemia mice using CRISPR/Cas9 gene-editing technology

β⁶⁵⁴-thalassemia is a prominent Chinese subtype of β-thalassemia, representing 17% of all cases of β-thalassemia in China. The molecular mechanism underlying this subtype involves the IVS-2-654 C→T mutation leading to aberrant β-globin RNA splicing. This results in an additional 73-nucleotide exon between exons 2 and 3 and leads to a severe thalassemia syndrome. Herein, we explored a CRISPR/Cas9 genome editing approach to eliminate the additional 73-nucleotide by targeting both the IVS-2-654 C→T and a cryptic acceptor splice site at IVS-2-579 in order to correct aberrant β-globin RNA splicing and ameliorate the clinical β-thalassemia syndrome in β⁶⁵⁴ mice. Gene-edited mice were generated by microinjection of sgRNA and Cas9 mRNA into one-cell embryos of β⁶⁵⁴ or control mice: 83.3% of live-born mice were gene-edited, 70% of which produced correctly spliced RNA. No off-target events were observed. The clinical symptoms, including hematologic parameters and tissue pathology of all of the edited β⁶⁵⁴ founders and their offspring were significantly improved compared to those of the non-edited β⁶⁵⁴ mice, consistent with the restoration of wild-type β-globin RNA expression. Notably, the survival rate of gene-edited heterozygous β⁶⁵⁴ mice increased significantly, and live-born homozygous β⁶⁵⁴ mice were observed. Our study demonstrated a new and effective gene-editing approach that may provide groundwork for the exploration of β⁶⁵⁴-thalassemia therapy in the future.     

Nanofused Hierarchically Porous MIL-101(Cr) for Enhanced Methyl Orange Removal and Improved Catalytic Activity

Hierarchically porous MIL-101(Cr) (H-MIL-101(Cr)) with meso/macro-pores was directly prepared via nanofusion progress by using butyric acid as a modulating agent. In the methyl orange (MO) adsorption experiments, H-MIL-101(Cr) showed a high adsorption capability of 369.8 mg g⁻¹, which was 1.52-fold greater than that of pristine MIL-101(Cr) (P-MIL-101(Cr)). While in the oxidation reaction of indene and 1-dodecene tests, H-MIL-101(Cr) presented much higher catalytic efficiency, with turnover frequency (TOF) values of 0.7242 mmol g⁻¹ min⁻¹ and 0.1492 mmol g⁻¹ min⁻¹, respectively, which were 28% and 34% greater than that in the case of P-MIL-101(Cr). Thus, compared with P-MIL-101(Cr), H-MIL-101(Cr) exhibited better removal efficiency and higher levels of activity in the oxidation reactions of indene and 1-dodecene. The unique structure of H-MIL-101(Cr) also contributed to its superior performance in these processes.     

Evaluation of the Immunogenicity in Mice Orally Immunized with Recombinant Lactobacillus casei Expressing Porcine Epidemic Diarrhea Virus S1 Protein

Porcine epidemic diarrhea (PED), characterized by diarrhea, vomiting, and dehydration, is an acute enteric infectious disease of pigs. The disease is caused by porcine epidemic diarrhea virus (PEDV), which infects the intestinal mucosal surface. Therefore, mucosal immunization through the oral route is an effective method of immunization. Lactic acid bacteria, which are acid resistant and bile-salt resistant and improve mucosal immunity, are ideal carriers for oral vaccines. The S1 glycoprotein of PEDV mediates binding of the virus with cell receptors and induces neutralizing antibodies against the virus. Therefore, we reversely screened the recombinant strain pPG-SD-S1/Δupp ATCC 393 expressing PEDV S1 glycoprotein by Lactobacillus casei deficient in upp genotype (Δupp ATCC 393). Mice were orally immunized three times with the recombinant bacteria that had been identified for expression, and the changes of anti-PEDV IgG and secreted immunoglobulin A levels were observed over 70 days. The results indicated that the antibody levels notably increased after oral administration of recombinant bacteria. The detection of extracellular cytokines on the 42nd day after immunization indicated high levels of humoral and cellular immune responses in mice. The above results demonstrate that pPG-SD-S1/Δupp ATCC 393 has great potential as an oral vaccine against PEDV.