中国脑型疟患者恶性疟原虫分离株裂殖子表面蛋白MSP1 第16- 17 区基因和MSP2 基因的分子克隆与鉴定

目的: 为设计研制安全有效的人脑型疟疫苗提供理论依据。方法: 根据MAD20 株裂殖子表面蛋白1 (MSP1) 和FC27 株裂殖子表面蛋白2 (MSP2) 基因编码区高度保守碱基设计并合成两对引物, 应用多聚酶链反应(PCR) 技术对5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株CMH/YN 和云南省盈江县农场CYJ/YN 分离株基因组DNA MSP1 第13- 17 区基因和MSP2 基因进行扩增, 并将扩增产物分别经EcoRI 和KpnI, BamHI 和HindIII 双酶切后, 分子定向克隆M13mp18 和M13mp19 载体, 转染大肠杆菌(E. coli) TG1, 从含X-gal 和IPTG 的LB平板上, 将随机筛选得到的单个无色噬菌斑经E. coli JM 103 扩增, 用碱裂解法抽提重组子复制型DNA (RFDNA ) 后, 再分别经EcoRI 和KpnI, BamHI 和HindIII 双酶切鉴定。结果: 证实重组子为编码脑型疟患者恶性疟原虫CMH/YN 和CYJ/YN 分离株MSP1 第16- 17 区基因和MSP2 基因分子克隆M13 载体。结论: 首次报道确证脑型疟患者恶性疟原虫CMH/YN 和CYJ/YN 分离株MSP1 第16- 17 区基因和MSP2 基因分别与MAD20 株MSP1 和FC27 株MSP2 相应基因完全一致。这些发现对研究预防人脑型疟疫苗和建立一种新型脑型疟恶性疟原虫检测方法具有重要意义。     

Clinical significance of the serum IgM and IgG to SARS‐CoV‐2 in coronavirus disease‐2019

ObjectiveTo explore the clinical value of serum IgM and IgG to SARS‐CoV‐2 in COVID‐19.Methods105 COVID‐19 patients were enrolled as the disease group. 197 non‐COVID‐19 patients served as the control group. Magnetic chemiluminescent immunoassay (MCLIA) was used to detect the IgM and IgG.ResultsThe peak of positive rates of SARS‐CoV‐2 IgM was about 1 week earlier than that of IgG. It reached to peak within 15–21 days and then began a slowly decline. The positive rates of IgG were increased with the disease course and reached the peak between 22 and 39 days. The differences in sensitivity of the three detection modes (IgM, IgG, and IgM + IgG) were statistically significant. The largest group of test cases (illness onset 15–21 days) showed that the positive rate of IgG was higher than IgM. Also, the sensitivity of IgM combined with IgG was higher than IgM or IgG. IgM and IgG were monitored dynamically for 16 patients with COVID‐19, the results showed that serological transformation of IgM was carried out simultaneously with IgG in seven patients, which was earlier than IgG in four patients and later than IgG in five patients.ConclusionThe detection of SARS‐CoV‐2 IgM and IgG is very important to determine the course of COVID‐19. Nucleic acid detection combined with serum antibody of SARS‐CoV‐2 may be the best laboratory indicator for the diagnosis of SARS‐CoV‐2 infection and the phrase and predication for prognosis of COVID‐19.     

创伤后感染致多器官功能障碍综合征患者血清β-hBD-2、OPN、pro-ADM和NPt水平的变化

目的：探讨多器官功能障碍综合征（multipleorgandysfunctionsyndrome,MODS）患者的炎症感染病理过程中炎性介质水平的变化。方法：血清人类B防御素-2（humanB．defensin-2,β—hBD-2）、骨桥蛋白（osteopontin,OPN）和新蝶呤（neoptefin,NPt）采用酶联免疫分析法（ELISA）测定,肾上腺髓质素前体（pro-adrenomeduUin,pro—ADM）检测采用化学免疫发光法。结果：四项血清标志物测定结果分别显示,对照组和确诊时组B-hBD-2、OPN、pro-ADM、NPt四种血清炎症介质标志水平均显著高于正常人组（P〈0．05,P〈0．01）,出院前组β-hBD-2、OPN水平显著高于正常人组,另两项指标均与正常人组比较无显著性差异（P均〉0．05）;同时确诊时组与对照组比较β—hBD-2、OPN、pro-ADM三项指标水平也显著升高（P〈0．05,P〈0．01）,NPt水平患者组呈略高,但无统计学意义（P〉0．05）;出院前组β-hBD-2、OPN、pro-ADM、NPt四种血清炎症介质标志水平均显著低于确诊时组（P〈0．05,P〈0．01）。结论：MODS患者的炎症感染病理过程中炎性介质水平显著升高,其测定对于临床抗感染治疗提供重要价值。     

Synthesis and Photovoltaic Performance of a [1,2,3]Triazolo[4,5‐g]quinoxaline‐Based Low‐Bandgap Polymer

A narrow‐bandgap conjugated polymer, PFDTBTzQ‐2OC1, is prepared by alternating [1,2,3]triazolo[4,5‐g]quinoxaline and 9,9‐didodecyl‐fluorene. With a bandgap of 1.63 eV, this polymer has wide absorption ranging from 300–760 nm in film. Bulk heterojunction solar cells fabricated by blending PFDTBTzQ‐2OC1 with [6,6]‐phenyl‐C₇₁‐butyric acid methyl ester exhibit a maximum power conversion efficiency of 1.31%, with a short‐circuit current density of 1.98 mA cm^–2, an open‐circuit voltage of 0.74 V, and a fill factor of 0.47.

     

Carbazole‐Substituted Triphenylamine and Diketopyrrolopyrrole Alternating Copolymer for Photovoltaic Cells

A new alternating conjugated polymer (PTCDPP) of carbazole‐substituted triarylamine and diketopyrrolopyrrole is prepared and characterized in detail. The polymer exhibits two strong absorption bands at 345 and 600 nm. With highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO) energy levels of ?5.13 eV and ?3.67 eV, PTCDPP displays an energy gap of 1.66 eV. PTCDPP‐based bulk heterojunction solar cells with a structure of fluorinated tin oxide (FTO)/TiO₂/PTCDPP:[6,6]‐phenyl‐C61‐butyric acid methyl ester (PCBM)/MoO₃/Ag are fabricated. The devices are optimized by adjusting the composition of the PTCDPP:PCBM active layer, thermal treatment, and addition of processing additives. The device based on PTCDPP:PCBM (1:4, w/w) shows a power conversion efficiency (PCE) of 2.31%, with a short‐circuit current of 4.17 mA cm^?2, an open‐circuit voltage of 0.79 V, and a fill factor of 0.35. The best cell performance (2.65% PCE) is achieved by using 1,8‐diiodooctane (3%, v/v) as a processing additive and annealing the active layer at 80 °C.

     

Effect of lemon peel flavonoids on UVB-induced skin damage in mice

By establishing an effective ultraviolet B (UVB) radiation model of skin damage in mice, the effect of lemon peel flavonoids (LPF) on skin damage was explored. UVB skin damage in UV-irradiated mice was simulated, and animal models were established. Serum parameters were measured using kits, skin sections were stained with hematoxylin–eosin (H&E) and Masson, and quantitative polymerase chain reaction (qPCR) was used to detect the expression of skin tissue-related mRNA. The experimental results showed that LPF increased the activity of catalase (CAT) and superoxide dismutase (SOD) oxidases in serum of mice with UVB-induced skin damage and decreased MDA, interleukin-1β (IL-1β), IL-6, IL-10, and tumor necrosis factor-alpha (TNF-α) levels. Pathological observation indicated that LPF alleviated the skin tissue lesions caused by UVB. LPF upregulated the mRNA expression of SOD1, SOD2, CAT, nuclear factor erythroid-2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and inhibitor of NF-κB alpha (IκB-α) and downregulated the expression of nuclear factor kappa B (NF-κB), p38 MAPK, and cyclooxygenase-2 (COX-2) in the skin tissue of skin-damaged mice. There was a greater protective effect of LPF on the skin as compared to vitamin C (VC) at the same application concentration, and the effect of LPF was positively correlated with the concentration. High performance liquid chromatography (HPLC) analysis showed that LPF contained five flavonoid compounds, namely isomangiferin, rutin, astragalin, naringin, and quercetin. We demonstrated that flavonoids from LPF exhibit an excellent skin protection effect with satisfactory application value.

By establishing an effective ultraviolet B (UVB) radiation model of skin damage in mice, the effect of lemon peel flavonoids (LPF) on skin damage was explored.     

Penile aseptic abscess in the cavernous body at the base of the penis:a case report

We report a case of aseptic abscess in the cavernous body at the base of the penis.In our clinical observation,the patient underwent puncture and drainage of the corpus cavernosum abscess,followed by surgical resection of the abscess wall,with the incisions closed layer by layer with primary suture.In addition,we paid attention to strengthening the postoperative management by using elastic bandages to wrap the penis intermittently to prevent edema;the incision would not be covered with dressings from the third day after the operation,so as to keep the incision site dry in an open way.During the period of indwelling of the catheter after the operation,we noticed the care of the external orifice of the urethra to reduce the occurrence of catheter-related infections.Finally,the patient was diagnosed with a penile aseptic abscess in the cavernous body at the base of the penis.The patient recovered well after surgery and was discharged 1 week later.At 1.5 years after the operation,the shape of the penis returned to normal,and the erectile function was normal.It was seen that good nursing concept is of great help for prognosis,which could avoid infection and edema,and is conducive to wound healing.     

Atrial performance in healthy subjects following high altitude exposure at 4100 m: 2D speckle-tracking strain analysis

The International Journal of Cardiovascular Imaging - High altitude (HA) exposure has been considered as a cardiac stress and might impair ventricular diastolic function. Atrial contraction is...     

我国儿童新型冠状病毒感染防控/诊疗方案纵览

儿童是新型冠状病毒的易感人群,易发生隐性感染。由于儿童群体的特殊性,疾病防控及诊疗工作面临着挑战。截至2020年2月29日,国内不同机构和学术团体已发布儿童新型冠状病毒感染防控/诊疗相关方案/建议/共识/指南(方案)29篇,其中防控方案17篇,防控和诊疗方案1篇,诊疗方案11篇。概括起来,儿童新型冠状病毒感染防控和诊治主要措施是:(1)严格按照"控制传染源""阻断传播途径""保护易感人群"的原则,在医院内、诊室和居家等场所执行隔离及防护、消毒措施,以有效防控儿童院内感染及家庭聚集性传播。(2)采取对症治疗为主,并避免滥用抗菌药物。(3)抗病毒治疗应根据患儿年龄、体重、临床分型、合并疾病等因素权衡利弊,发病早期推荐试用干扰素。(4)中医药治疗应遵循辨证论治的原则。(5)重症患儿可酌情采用糖皮质激素、丙种球蛋白、无带毒血浆治疗。