首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   26817篇
  免费   2657篇
  国内免费   1895篇
耳鼻咽喉   168篇
儿科学   340篇
妇产科学   376篇
基础医学   3254篇
口腔科学   479篇
临床医学   3607篇
内科学   4325篇
皮肤病学   244篇
神经病学   1570篇
特种医学   916篇
外国民族医学   21篇
外科学   2700篇
综合类   4153篇
现状与发展   5篇
一般理论   1篇
预防医学   1997篇
眼科学   655篇
药学   2762篇
  23篇
中国医学   1465篇
肿瘤学   2308篇
  2024年   169篇
  2023年   516篇
  2022年   1178篇
  2021年   1521篇
  2020年   1136篇
  2019年   990篇
  2018年   1020篇
  2017年   949篇
  2016年   837篇
  2015年   1261篇
  2014年   1486篇
  2013年   1356篇
  2012年   1976篇
  2011年   2175篇
  2010年   1250篇
  2009年   982篇
  2008年   1368篇
  2007年   1276篇
  2006年   1286篇
  2005年   1280篇
  2004年   897篇
  2003年   721篇
  2002年   698篇
  2001年   559篇
  2000年   606篇
  1999年   698篇
  1998年   414篇
  1997年   402篇
  1996年   307篇
  1995年   273篇
  1994年   198篇
  1993年   156篇
  1992年   224篇
  1991年   179篇
  1990年   154篇
  1989年   153篇
  1988年   132篇
  1987年   123篇
  1986年   109篇
  1985年   98篇
  1984年   45篇
  1983年   33篇
  1982年   20篇
  1981年   18篇
  1980年   16篇
  1979年   18篇
  1978年   12篇
  1974年   9篇
  1971年   10篇
  1970年   9篇
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
71.
目的:观察CBN对脑缺血再灌注小鼠的线粒体损伤的影响。方法:分离提纯脑缺血再灌注小鼠脑的线粒体。用分光光度法检测线粒体的膜肿胀度,用激光拉曼光谱检测脑线粒体的生物大分子的变化。结果:小鼠脑缺血再灌注后。其线粒体出现膜肿胀,CBN50、100mg/kg iv对此有明显的抑制作用。各组脑线粒体的Raman和IR光谱基本一致。CBN组与假手术组比较接近。模型组只是在量上有一些差异。结论:CBN对脑缺血再灌注小鼠的线粒体损伤的有一定的保护作用。  相似文献   
72.
BACKGROUND AND PURPOSE: Anti-Helicobacter pylori triple therapy is effective for healing duodenal ulcer (DU) diseases and reducing disease recurrence. However, multifocal duodenal erosions or shallow ulcers may develop after triple therapy. The purpose of this study was to investigate the incidence and outcome of duodenal erosions that developed after triple therapy. METHODS: A total of 106 Taiwanese with active DU and with H. pylori infection were enrolled in this study. All patients received anti-H. pylori triple therapy (i.e., 2 weeks of antimicrobial agents combined with treatment for 4 to 6 weeks with acid suppression agents). Follow-up endoscopy was performed immediately after stopping treatment. The incidence of multifocal erosions or shallow ulcers over the bulb and/or second portion of the duodenum was studied. Additional acid suppression agent was given for 4 weeks whenever duodenal erosions or shallow ulcers were found. RESULTS: Out of 106 patients, 11 (10.4%) were found to have multifocal duodenal erosions and/or shallow ulcers on the duodenal bulb and/or second portion of the duodenum at the end of treatment. Ten of the 11 patients with newly developed erosions had healed DU in the S1 or S2 stage, and all 11 had successful H. pylori eradication. The duodenal erosions and/or shallow ulcers of these 11 patients were healed after an additional 4 weeks of histamine-2-receptor antagonist therapy. CONCLUSIONS: Multifocal duodenal erosions and/or shallow ulcers were noted in around 10% of Taiwanese DU patients who received anti-H. pylori triple therapy. An additional 4 weeks therapy with acid suppression agents healed these lesions.  相似文献   
73.
In the humoral immune response to an invasion of foreign antigens, B cells differentiate into low-affinity antibody-forming cells (AFCs) that mainly secrete IgM or, through germinal center (GC) formation, into high-affinity AFCs that secrete IgG-class antibodies with a higher affinity for the antigen. Previous studies have established the suppressive effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on low-affinity antibody responses to antigens. However, whether and how TCDD affects the high-affinity antibody response to antigens has not yet been clarified. In this paper we investigate the effects of TCDD on GC formation, high-affinity AFC generation, and high-affinity antibody production in the primary humoral immune response. C57BL/6 mice were orally administered 0 or 20 microg/kg of TCDD and subsequently immunized with alum-precipitated ovalbumin (OVA) on day 0. Then the GC formation in the spleen and OVA-specific antibodies in the plasma, was evaluated until day 14 postimmunization. TCDD exposure reduced the production of OVA-specific IgG1 on days 10 and 14. GC formation in the spleen was also suppressed by TCDD exposure, and the suppression persisted from day 7 until day 14. In TCDD-administered mice, on day 7, cellular proliferation in the GCs was significantly suppressed, although apoptosis was not markedly affected. In order to measure high-affinity antibody and high-affinity AFCs, the mice were administered TCDD followed by immunization with alum-precipitated (4-hydroxy-3-nitrophenyl) acetyl linked to chicken gamma-globulin (NP-CG). The frequency of high-affinity NP-specific AFCs that bind to low-haptenated antigen was clearly shown to be reduced in the spleen on days 10 and 14. Furthermore, the high-affinity anti-NP IgG1 levels on days 10 and 14 postimmunization were significantly reduced by TCDD exposure. Taken together, the results of this paper demonstrate that TCDD exposure inhibits the generation of high-affinity AFCs and high-affinity antibody production during the primary humoral immune response and suggest that these alterations were caused by the suppression of antigen-responding B-cell proliferation induced by TCDD during GC formation.  相似文献   
74.
The SH2 domain-containing inositol 5'-phosphatase (SHIP) is crucial in hematopoietic development. To evaluate the possible tumor suppressor role of the SHIP gene in myeloid leukemogenesis, we examined primary leukemia cells from 30 acute myeloid leukemia (AML) patients, together with eight myeloid leukemia cell lines. A somatic mutation at codon 684, replacing Val with Glu, was detected in one patient, lying within the signature motif 2, which is the phosphatase active site. The results of an in vitro inositol 5'-phosphatase assay revealed that the mutation reduced catalytic activity of SHIP. Leukemia cells with the mutation showed enhanced Akt phosphorylation following IL-3 stimulation. K562 cells transfected with the mutated SHIP-V684E cDNA showed a growth advantage even at lower serum concentrations and resistance to apoptosis induced by serum deprivation and exposure to etoposide. These results suggest a possible role of the mutated SHIP gene in the development of acute leukemia and chemotherapy resistance through the deregulation of the phosphatidylinositol-3,4,5-triphosphate (PI(3,4,5)P3)/Akt signaling pathway. This is the first report of a mutation in the SHIP gene in any given human cancer, and indicates the need for more attention to be paid to this gene with respect to cancer pathogenesis.  相似文献   
75.
76.
77.
为确立一种原发喉室的跨声门癌,选择肿瘤主体以喉室为中心经全喉切除手术的标本50例,火棉胶包埋,连续切片观察。结果发现①跨声门癌的肿瘤原发部位在喉室,50例中有T23例。②肿瘤以深层浸润为主占90%(45/50),易侵及声门旁间隙(82%,41/50)和甲状软骨(64%,32/50),临床分期与病理分期不符达48%(24/50)。结论:原发喉室的跨声门癌是声门上型癌的一种特殊类型。  相似文献   
78.
HPLC测定佛手增乳膏中阿魏酸的含量   总被引:4,自引:0,他引:4  
潘锡强  刘宇翔 《中成药》1998,20(11):12-13
佛手增乳膏经乙醚,2%碳酸钠液萃取后,用HPLC测定阿魏酸的含量。方法简便,快速,准确。  相似文献   
79.
喉近全切除喉功能重建术   总被引:14,自引:1,他引:14  
目的 为了减少喉全切除率并重建喉功能。方法 自1991 ̄1996年作喉近全切除喉功能重建术19例。男8例,女11例。年龄最大74岁,最小40岁,平均57.6岁。临床分期Ⅱ期2例,Ⅲ期9例,Ⅳ期8例。手术特点是:切除舌骨,保留环状软骨及一侧杓状软骨,将五状软骨前缘与舌根切缘吻合,增强了舌根对新喉口的遮盖作用,减轻了误咽.结果 全部病例术后发音功能良好,多数病例误咽不重。5例拔除套管经喉呼吸。14例新  相似文献   
80.
MEDIASTINAL enterogenous cysts are congen-ital anomalies rarely seen in the pediatric agegroups.Most children are first admitted tohospital due to respiratory symptoms.Neonates and littleinfants often have the symptom of respiratory distress,while a few a…  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号