纤维组织粘合剂的实验研究

本文应用“冷沉淀”法制备纤维组织粘合剂。对纤维组织粘合剂中主要成份和含量进行了测定,并测定了主要理化性质,建立了动物实验模型,观察了实验兔对纤维粘合剂的反应。     

Potential contribution of nuclear factor-kappaB to cerebral vasospasm after experimental subarachnoid hemorrhage in rabbits.

Nuclear factor-kappaB (NF-kappaB) plays a key role in inflammation, which is involved in the development of cerebral vasospasm after subarachnoid hemorrhage (SAH). In the present study, we assessed the potential role of NF-kappaB in regulation of cerebral vasospasm. Nuclear factor-kappaB DNA-binding activity was measured in cultured vascular smooth muscle cells (VSMCs) treated with hemolysate and pyrrolidine dithiocarbamate (PDTC, 80 micromol/L), an inhibitor of NF-kappaB. Forty-two rabbits were divided into three groups: control, SAH, and PDTC groups (n=14 for each group). The caliber of the basilar artery was evaluated. Nuclear factor-kappaB DNA-binding activity and the gene expression levels of cytokines and adhesion molecules in the basilar artery were measured. Immunohistochemical study was performed to assess the expression and localization of tumor necrosis factor (TNF)-alpha, intercellular adhesion molecule (ICAM)-1, and myeloperoxidase (MPO). It was observed that NF-kappaB DNA-binding activity was significantly increased by treatment with hemolysate in cultured VSCMs, but this increase was suppressed by pretreatment with PDTC. Severe vasospasm was observed in the SAH group, which was attenuated in the PDTC group. Subarachnoid hemorrhage could induce increases of NF-kappaB DNA-binding activity and the gene expression levels of TNF-alpha, interleukin (IL)-1 beta, ICAM-1, and vascular cell adhesion molecule (VCAM)-1, which were reduced in the PDTC group. Immunohistochemical study demonstrated that the expression levels of TNF-alpha, ICAM-1, and MPO were all increased in the SAH group, but these increases were attenuated in the PDTC group. Our results suggest that NF-kappaB is activated in the arterial wall after SAH, which potentially leads to vasospasm development through induction of inflammatory response.     

开放手术观察腰椎间盘突出症溶核失败45例分析

目的 通过开放手术观察分析椎间盘髓核化学溶解术治疗腰椎间盘突出症失败原因。方法 收集溶核失败的腰椎间盘突出症45例行开放手术治疗。结果 术中见45例硬膜外脂肪完全消失，43例髓核未见溶解，2例髓核溶解呈糊状但未被吸收，21例伴有侧隐窝狭窄，15例突出物与神经根粘连，20例黄韧带增厚，2例椎管骨性狭窄，14例突出物钙化。结论 腰椎间盘突出症病变间隙合并有侧隐窝狭窄、神经根粘连、椎管狭窄、突出物钙化等，不是溶核治疗的适应证。     

益母煎辅助早期妊娠药物流产50例

米非司酮配伍米索前列醇终止早孕药物流产已被广泛应用于临床，其完全流产率达90％以上，有效、安全、对身体损伤轻，许多妇女愿意选择药物流产。但药物流产存在阴道流血量多，持续时间长，易发生不全流产及致贫血、休克等弊端。笔者2003年8月～2006年10月，在口服米非司酮的第1天使用自拟中药益母煎治疗早期妊娠药物流产50例，起效快，排出孕囊完整，出血时间短，加速了流产时间，提高了疗效，现报道如下。     

Determination of the conjunctival goblet cell density in 43 normal subjects

The goblet cell density in the nasai inferior fornical conjunctiva was determined in 43 normal subjects (57 eyes). The mean number of goblets per 100 epithelial cells was found to be 10.17 +/- 2.81 in the young averaging 25 years and 5.25 +/- 3.38 in the aged averaging 62 years (P less than 0.01). The relation between the morphological features of the epithelial cells and the number of goblet cells was discussed.     

ΔNp63蛋白在膀胱移行上皮癌中的表达及其临床意义

目的 :探讨 p5 3基因家族新成员截短型p6 3(△Np6 3)在膀胱癌组织中的表达及其意义。 方法 :采用免疫组织化学SP法检测 4 0例膀胱移行上皮癌 (TCC)、6例膀胱内翻性乳头状瘤和 8例正常膀胱移行上皮中△Np6 3的表达 ,并分析△Np6 3表达与膀胱癌病理类型、临床分期的关系。 结果 :正常膀胱移行上皮、膀胱内翻性乳头状瘤、TCC中△Np6 3的阳性表达率分别为 37.5 % (3/ 8)、6 6 .7% (4/ 6 )、10 0 % (40 / 4 0 ) ,组间差异有统计学意义 (P <0 .0 1)。TCCG3 级与G2 级△Np6 3的强阳性、中度阳性表达率显著高于G1级 (P <0 .0 1)。Ta～T1期以△Np6 3弱阳性为主 (6 6 .7% ) ,随TCC浸润程度的增加 ,△Np6 3染色强度逐渐增强。T2 期△Np6 3强阳性表达率为 35 .3% ,T3 ～T4期增至 6 3.6 %。结论 :△Np6 3在TCC中高表达 ,与TCC病理分级、临床分期密切相关 ;△Np6 3可能参与TCC的发生、发展 ,是评估TCC预后的潜在因素之一。     

再谈提高肝门胆管癌切除率有关的手术策略与技术问题

肝外胆管癌近年来发现率有增长趋势。其中胆管上段癌，常称肝门部胆管癌，又名Klaskin＇s瘤，最为常见。由于所处位置特殊，易侵犯肝门主要血管，治疗困难，过去屡放弃根治手术。近年来由于手术技巧的改进和经验的积累，肝门部恶性肿瘤切除有了明显提高，预后亦有所改善。本文仅就提高肝门胆管癌切除的有关手术策略与肝门外科技术的问题讨论于后。     

前列腺素E1对大鼠肝脏缺血再灌注损伤的保护作用

目的 探讨前列腺素E1（PGE1）对肝脏 因再灌注损伤的保护作用。方法 制作常温下大鼠部分肝叶缺血再灌注模型，于缺血前经门静脉给予PGE1，45min后恢复血流灌注，并于1h后取门静脉血测定血清谷草转氨酶（GOT）、谷丙转氨酶（GPT）、乳酸脱氢酶（LDH）、肿瘤坏死因子－α（TNF－α）及内皮素1（ET－1），同时取缺血肝叶行病理组织学检查。结果 缺血再灌注组GOT、GPT、LDH及TNF－α和ET－1均明显高于正常对照组，PGE1组则明显低于缺血再灌注组。PGE1组的肝脏病理组织学改变明显轻于缺血再灌注组，并接近正常对照组。结论 PGE1对肝缺血再灌注具有保护作用。     

中国心理咨询发展的历史回顾与前景趋势

目的:回顾心理咨询在中国产生、发展的历史和现状,提出存在的问题和不足,展望21世纪心理咨询发展的前景和趋势。资料来源:通过整理专家专题讲座笔记和手工查阅与心理咨询相关的资料熏检索词“心理咨询”熏并限定文章语言种类为中文。资料选择:对资料进行初审,选取心理咨询文献开始查找全文。纳入与心理咨询相关的研究。资料提炼:根据国内外关于心理咨询的研究资料和印刷品资源中心理咨询相关资料进行归纳和概括,提取相关资料并进行整合。纳入与国外心理咨询历史发展和生态学有关,与中国心理咨询发展与现状有关的资料。资料综合:心理咨询在发达国家虽然已有近百年的历史,但在中国才刚刚起步阶段,广大心理咨询工作者经过近20多年的不懈地努力,心理咨询理论和方法虽然有了雏形,还需要进一步的完善。结论:借鉴国外心理咨询发展的新理论和新方法,是发展中国心理咨询理论与方法的有效途径。可以认为,生态观将成为21世纪心理咨询发展的大趋势之一。     

腹腔镜在胰腺外科的应用现状

在胰腺外科领域,由于解剖复杂、手术难度大等原因,使得腹腔镜手术发展相对缓慢,开展有限,现就其现状作一综述.