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991.
目的了解煤工尘肺患者下呼吸道产金属内酰胺酶的铜绿假单胞菌(PA)的感染状况及其耐药特性。方法分离下呼吸道感染的煤工尘肺患者痰液标本中的PA,根据药敏试验,筛选可疑产金属内酰胺酶PA,最后依据2-巯基丙酸双纸片法鉴定产金属内酰胺酶PA。结果40株PA中共筛选出10株可疑PA,最终确定产金属内酰胺酶PA6株。这些菌株均有很强的耐药性,对亚胺培南、头孢菌素、含酶抑制剂类抗菌药物全部耐药;对阿米卡星、环丙沙星、左氧氟沙星、庆大霉素等药物部分耐药。产金属内酰胺酶PA的耐药性远强于不产金属内酰胺酶的PA。结论产金属内酰胺酶PA的耐药性很强,应加强煤工尘肺患者产金属内酰胺酶PA的检测,以利于指导治疗和控制感染。 相似文献
992.
目的为了及时准确地掌握各类传染病的漏报情况,为制订传染病防治策略提供理论依据。方法每年对区、市、系统、乡级医疗机构疫情报告情况进行调查,计算漏报率。结果共调查传染病患者4069例,漏报130例.总平均漏报率为3.19%。年漏报率最低为0.53%;最高为10.39%。漏报病种最高为细菌性痢疾和病毒性肝炎.分别占总漏报数的53.85%和26.15%。结论领导重视、提高业务人员的能力和基层防保队伍的建设,是传染病疫情报告和管理工作顺利开展的保证。 相似文献
993.
[目的]了解出国劳务人员中传染病感染情况,为口岸疾病监测工作提供科学依据。[方法]对大连口岸13902名各类出国劳务人员按照《国际旅行健康检查证明书》逐项进行临床检查和实验室检验。[结果]检出各类疾病2742例,检出率为19.72%。其中传染性疾病548例,占病例总数的19.99%;非传染性疾病2194例,占病例总数的80.01%。[结论]出国劳务人员是国际旅行者中的特殊群体,由于长期的集体生活,极易患各种疾病,尤其是传染性疾病。应加强对出国劳务人员的传染病监测体检,并对劳务人员的健康状况进行评估,使他们了解自己的健康状况,防患于未然,提高自我保护意识,有效控制传染病在国内、国际间的传播。 相似文献
994.
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997.
目的:尝试利用科学性之方法探讨耳穴磁疗对老人失眠症之效果.方法:120位60岁或以上之老年患者,随机分配入三组.第一组及第二组为对照组,分别贴有灯芯或王不留行籽(不加压);第三组为实验组,贴有每粒含66高斯之磁珠(不加压).为针对老年患者常见之脏腑功能衰弱而致失眠,各人均选取相同之穴位:神门、心、肾、肝、脾、皮质下及枕,疗程共3周.结果:疗程完毕后,使用磁珠作耳疗之实验组于治疗前后的睡眠效果指数有明显之改进(P<0.05),显效17%,有效为65%,总有效率为82%.此外,利用多元回归之反向淘汱法分析女性参加者之数据,发现利用磁珠作耳疗及参加者年龄对预测睡眠效果指数达显著水准(F3,106=9.04,P<0.001,R2=0.20).结论:耳穴磁疗能有效地改善老人失眠症. 相似文献
998.
Epitopes of human brain acetylcholinesterase 总被引:4,自引:0,他引:4
The main purpose of the present work was to identify B-cell epitopes on human brain acetylcholinesterase (AChE) by the synthetic peptide approach. Five hundred and seventy-four decapeptides comprising amino acids No. n to n+9 (where n denotes the residue number of the 583 amino acids in the primary structure of human brain AChE and is an integer in the range 1-574) were synthesized, using the multipin combinatorial chemical synthesis technique, and biotinylated. Epitopes of human brain AChE were detected by enzyme-linked immunosorbent assay (ELISA) and compared with the predicted epitopes of human AChE by 'Goldkey' software. Among 574 synthetic decapeptides, 47 decapeptides at 11 antigenic regions showed immunoreactivity with mouse anti-human brain AChE polyclonal antibodies. The minimum sequence of epitope was defined at every antigenic region explored. The locations and sequences of the former ten continuous epitopes at the 11 antigenic regions of the human brain AChE had been identified as follows: TPVLVWIY (112-119), RTVLVSMNY (143-151), LLDQRLALQW (173-182), RRATQLAH (246-253), VFRFSFVPV (294 approximately 302), KDEGSYFLVY (332-341), RVYA (424-427), LMRY (476-479), KAPQWPPY (496-503), GLRAQACAFW (523-532). The rate of hits of the predicted epitopes from the software came out at 33%. In our work, the epitopes of human AChE have been mapped by purified polyclonal antibody at eleven distinct sites in the primary structure. 相似文献
999.
OBJECTIVE: Release of oxygen free radicals occurs following cerebral ischemia. Studies show that oxygen free radicals mediate ischemic brain injury. CoQ10 is a potent free radical scavenger and may offset brain injury associated with reperfusion. We tested exogeneous CoQ10 as a neuroprotectant in rats following both global and focal ischemic insults. METHODS: Rats were subjected to either 4-vessel occlusion ischemia (4-VO, 10 min occlusion, 7-day survival) or middle cerebral artery occlusion (MCAO, 120 min-occlusion, 22.5 h survival). Regional cerebral blood flows (rCBF) and physiological variables such as blood pressure, pO2, pCO2, plasma glucose and hematocrit were monitored and measured in focal ischemia. The animals were randomized to receive treatments of either phosphate buffered saline (PBS) vehicle or CoQ10 following global or focal ischemia. Injection times were at the end of ischemia and 3 h later for both models of ischemia. Histological outcomes are expressed as a percentage of hippocampal CA(1) cell injury in global ischemia or percentage of cortical infarct over that of non-ischemic hemisphere in focal ischemia. RESULTS: In global ischemia, animals treated with PBS vehicle and CoQ10 had 86+/-5% (n=8) and 83+/-10% (n=8), respectively, of hippocampal CA(1) cell injury (P>0.05). The percentage of infarct volumes in animals following focal ischemia were 23+/-9% (control, n=10) and 25+/-9% (CoQ10, n=10). There were no temperature or physiological differences between the two treatment groups. CONCLUSION: Acute treatment with CoQ10 via intraperitoneal injection does not prevent neuronal injuries following global and focal ischemia. 相似文献
1000.
Extensive studies have been performed to evaluate different factors that may affect on spinal interbody fusion, but the role of intervertebral disc tissue in the fusion process remains unclear. To study the influence of intervertebral disc tissue on osteoblast metabolism, we harvested disc tissue from patients who had undergone spinal surgery. The nucleus pulposus and annulus fibrosus were separately co-cultured with osteoblast-like cells SaOS-2 by means of culture inserts or organ culture. We assayed alkaline phosphoatase activity, 3H-thymidine incorporation into the DNA, and production of collagen type I, IL-1beta, IL-6, IL-10, and TNF-alpha. Exposure of the nucleus pulposus (NP) to osteoblast-like cells revealed stimulation of alkaline phosphatase production, 3H-thymidine incorporation and collagen type I production. Exposure of the annulus fibrosus (AF) stimulated 3H-thymidine incorporation and collagen type I production, but did not affect ALP activity. IL-6 was detected after application of NP and AF. Interleukin IL-10, IL-1beta and TNF-alpha were all below detection levels after application of disc tissue. Our findings show that frozen disc tissue stimulates the metabolism of osteoblast-like cells in vitro. 相似文献