原发性胆汁性肝硬化-自身免疫性肝炎重叠综合征临床病理研究

目的探讨并比较自身免疫性肝炎(AIH)、原发性胆汁性肝硬化(PBC)及原发性胆汁性肝硬化-自身免疫性肝炎重叠综合征(AIH-PBC重叠综合征)的临床、生化及病理特征。方法对具有肝穿刺标本的14例AIH-PBC重叠综合征、26例Ⅰ型AIH和29例PBC(Scheuer分期Ⅰ、Ⅱ期)患者进行比较,重点分析AIH-PBC重叠综合征的临床表现、生化特点及病理特点。结果 3组患者的性别、年龄及临床特点无显著差异;AIH-PBC重叠综合征血清碱性磷酸酶(ALP)、γ-谷氨酰转肽酶(GGT)、抗线粒体抗体(AMA)、AMA-M2阳性率明显高于AIH组(P<0.05);而丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、γ-球蛋白、免疫球蛋白IgG以及抗核抗体(ANA)或抗平滑肌抗体(SMA)阳性率明显高于PBC(P<0.05或P<0.01)。肝组织学示界面炎/碎屑样坏死、小叶内炎症及胆管病变。结论 AIH-PBC重叠综合征临床、血清学及组织病理学表现出AIH和PBC双重特征,不同于单纯的PBC和AIH。     

HLA-DM基因遗传多态性对系统性红斑狼疮易感性的影响

目的　探索 HL A- DM等位基因频率与基因型分布对系统性红斑狼疮 （SL E）易感性的影响。方法　用多聚酶链反应 -限制性片断长度多态性 （PCR- RFL P）确定 DM的基因型 ,用病例对照研究的方法探索 DM与 SL E的关联性。结果　检测到的 7种 DMA与 DMB等位基因和 4 2种单体型在 SL E组与正常对照组分布一致。DMB* 0 10 2 / 0 10 3基因型在对照组的分布频率明显高于 SL E组 （P <0 .0 5 ） ,但校正后差异无显著性 ,其余 13种基因型在两组差异均无显著意义。结论　 HL A-DM基因的遗传多态性对 SL E的发病没有直接影响     

海星甾醇抗实验性心律失常的作用

目的：研究海星甾醇C01对实验性心律失常的拮抗作用。方法：麻醉大鼠股静脉快速iv5?Cl2诱发室颤（VF）,记录大鼠的VF发生和死亡数,小鼠尾静脉ivCaCl2-Ach混合液（10ml/kg）诱发小鼠房颤（扑）,记录房颤（扑）的发生率;麻醉大鼠冠脉结扎再灌注诱发心律失常,记录结扎5min和再灌期各时间点的心律失常发生率,并比较ST段的变化,电刺激致家兔室颤,记录室颤阈值。结果：海星甾醇C801显著降低了CaCl2诱发VF的发生率,抑制小鼠房颤（扑）的发生,对大鼠结扎期 再灌期各个时间点都有显著的抗心律失常的作用,减轻结扎造成的心肌缺血及再灌所致的心肌损伤,中高剂量组可显著提高家兔室颤阈值,降低VF的发生。结论：C01能对抗多个性和房性心律失常的实验模型,提示C01具有广泛的抗心律失常作用。     

Effects of the tropical ginger compound, 1'-acetoxychavicol acetate, against tumor promotion in K5.Stat3C transgenic mice

ABSTRACT: The purpose of the current study was to determine whether a tropical ginger derived compound 1'-acetoxychavicol acetate (ACA), suppresses skin tumor promotion in K5.Stat3C mice. In a two-week study in which wild-type (WT) and K5.Stat3C mice were co-treated with either vehicle, ACA, galanga extract, or fluocinolone acetonide (FA) and tetradecanoyl phorbol acetate (TPA), only the galanga extract and FA suppressed TPA-induced skin hyperproliferation and wet weight. None of these agents were effective at suppressing pTyr705 Stat3 expression. However, ACA and FA showed promising inhibitory effects against skin tumorigenesis in K5.Stat3C mice. ACA also suppressed phospho-p65 NF-kappaB activation, suggesting a potential mechanism for its action.     

Suppression of Human Liver Cancer Cell Migration and Invasion via the GABAA Receptor

Objective To investigate the roles of theγ-aminobutyric acid(GABA) in the metastasis of hepatocellular carcinoma(HCC) and to explore the potential of a novel therapeutic approach for the treatment of HCC. Methods The expression levels of GABA receptor subunit genes in various HCC cell lines and patients’ tissues were detected by quantitative real-time polymerase chain reaction and Western blot analysis.Transwell cell migration and invasion assays were carried out for functional analysis.The effects of GABA on liver cancer cell cytoskeletal were determined by immunofluorescence staining. And the effects of GABA on HCC metastasis in nude mice were evaluated using an in vivo orthotopic model of liver cancer. Results The mRNA level of GABA receptor subunits varied between the primary hepatocellular carcinoma tissue and the adjacent non-tumor liver tissue.GABA inhibited human liver cancer cell migration and invasion via the ionotropic GABAa receptor as a result of the induction of liver cancer cell cytoskeletal reorganization.Pretreatment with GABA also significantly reduced intrahepatic liver metastasis and primary tumor formation in vivo. Conclusions These findings introduce a potential and novel therapeutic approach for the treatment of cancer patients based on the modulation of the GABAergic system.     

息烽县人民医院1135份住院病历抗菌药物应用分析

目的:了解我院住院患者抗菌药物使用情况,以便为临床提供抗菌药物信息。方法:以随机抽样法抽取2010年7月—2011年4月住院病历1 135份,采用回顾性分析方法,对病历中抗菌药物的使用情况进行归纳、分析、总结。结果:1 135份住院病历中,使用抗菌药物799份(占70.3%),其中单一用药542份(67.83%)、二联用药255份(31.92%)、三联用药2份(0.25%)。结论:我院抗菌药物使用工作需加强,特别是临床医师应加强对《抗菌药物临床应用指导原则》的学习,提高医院抗菌药物使用的安全性、合理性和有效性,减少细菌耐药性的产生,降低患者的医药费用。     

针刺阳陵泉结合刺络拔罐治疗肩周炎的临床观察

目的：观察针刺阳陵泉结合刺络拔罐法治疗肩周炎的临床疗效。方法：将80例患者随机分为治疗组42例,对照组38例,治疗组采用针刺阳陵泉、痛点刺络拔罐,对照组采用针刺肩三针的方法。结果：治疗组与对照组比较具统计学意义(P<0.05)。结论：针刺阳陵泉结合刺络拔罐治疗肩周炎疗效显著。     

慢衰灵口服液治疗心肾阳虚型心脏病左室舒张功能障碍患者的临床观察

目的：探讨慢衰灵口服液对心肾阳虚型心脏病左室舒张功能障碍的影响。方法：将90例心肾阳虚型心脏病左室舒张功能障碍患者随机分为常规治疗组(简称常疗组)和中药治疗组(简称中疗组),每组45例,常疗组每日给予强心、利尿、扩冠等常规抗心衰西药治疗,中疗组除常规治疗外每日予慢衰灵口服液200ml,早晚2次分服,服药4个月后,复查心力衰竭总积分(HFC)及进行心功能测定。结果：两组治疗后较治疗前HFC降低(P<0．05或P<0．01),且中疗组优于常疗组(P<0．05);左室舒张早期快速充盈峰值流速(Emas)加快,左房收缩期血流峰值流速(Amas)减慢以及Emas／Amas的比值(E／A)升高均有改善(P<0．05或P<0．01),中疗组优于常疗组(P<0．05)。中疗组治疗前后左室等容舒张时间(IVRT)缩短差异显著(P<0．01),明显优于常疗组(P<0．05)。62例舒张、收缩功能障碍的混合性心衰(LVSD+LVDD)患者中,中疗组在增加射血分数(EF)、心输出量(CO)和左心室后壁增厚率(△T)方面均明显优于常疗组(P<0．05)。结论：慢衰灵口服液能改善心肾阳虚型心脏病左心室舒张功能障碍患者的心功能,减轻临床症状。     

A chemical biology screen identifies glucocorticoids that regulate c-maf expression by increasing its proteasomal degradation through up-regulation of ubiquitin

The oncogene c-maf is frequently overexpressed in multiple myeloma cell lines and patient samples and contributes to increased cellular proliferation in part by inducing cyclin D2 expression. To identify regulators of c-maf, we developed a chemical screen in NIH3T3 cells stably overexpressing c-maf and the cyclin D2 promoter driving luciferase. From a screen of 2400 off-patent drugs and chemicals, we identified glucocorticoids as c-maf-dependent inhibitors of cyclin D2 transactivation. In multiple myeloma cell lines, glucocorticoids reduced levels of c-maf protein without influencing corresponding mRNA levels. Subsequent studies demonstrated that glucocorticoids increased ubiquitination-dependent degradation of c-maf and up-regulated ubiquitin C mRNA. Moreover, ectopic expression of ubiquitin C recapitulated the effects of glucocorticoids, demonstrating regulation of c-maf protein through the abundance of the ubiquitin substrate. Thus, using a chemical biology approach, we identified a novel mechanism of action of glucocorticoids and a novel mechanism by which levels of c-maf protein are regulated by the abundance of the ubiquitin substrate.     

应用Meta分析有效估计人群归因危险度百分比的探讨

人群归因危险度百分比 (populationattributableriskproportion ,PARP)是总体人群中某种疾病归因于某种因素的暴露所引起的发病 (死亡 )占全部发病 (死亡 )的百分比 ,反映该因素所引起的发病 (死亡 )占全部发病 (死亡 )的比重。通过PARP可了解各危险因素对人群中某疾病的发病所产生的影响 ,亦即消除某危险因素后 ,所产生的对预防该疾病的效果将占有多大比重。它能够为卫生政策的制订提供依据 ,有着重要的公共卫生的实际意义。目前常用的估计PARP的方法有两种 :一种是利用全国人群抽样调查获得的人群总暴露率来估计 ;另一种是利用某地区…