Effect of GABA on sperm acrosome reation in antisperm antibody positive patients

To investigate the effect of gamma-aminobutyric acid (GABA) on the rate of sperm acrosome reaction both in normal and antisperm antibody (AsAb) positive men. Methods: The sperm acrosome reaction was tested with triplestain technique in two groups of 18 men each. Results: (1) GABA increased the rate of sperm acrosome reaction both in normal and AsAb positive subjects (P<0.01); (2) GABA increased the Na~ -K~ -ATPase activity of sperm (P<0.01); (3)GABA increased the Ca~(2 )-ATPase activity of sperm (P<0.05); (4) GABA decreased the production of MDA and oxygen free radicals of sperm. Conclusion: GABA could regulate the rate of sperm acrosome reaction. (Chin J Andro12002; 16: 355)     

季节性面部接触性皮炎患者血清总IgE、特异性IgE及其皮肤划痕试验的相互关系

采用ＩＲＭＡ对季节性面部接触性皮炎患者进行血清总ＩｇＥ测定，同时用南京地区常见致敏花粉进行皮肤划痕试验及ＥＬＩＳＡ检测血清特异性ＩｇＥ。结果：血清总ＩｇＥ与特异性ＩｇＥ有明显相关（Ｐ＜０．０１）、符合率为７０．７％，血清总ＩｇＥ与皮肤划痕试验有明显相关（Ｐ＜０．００５）、符合率为７５．６％，皮肤划痕试验与血清特异。性ＩｇＥ有明显相关（Ｐ＜０．００５）、符合率为７５．６％。三者总符合率为６４．９％。     

Targeting NF-κB c-Rel in regulatory T cells to treat corneal transplantation rejection

The relevance of Tregs in the induction of tolerance against corneal allografts has been well established. Although it is well known that the conversion of Tregs into effector-like cells contributes to the loss of corneal immune privilege, the underlying mechanism is still not fully understood. Using heterologous penetrating keratoplasty model, we found that Tregs from corneal allograft rejected mice (inflam-Tregs) exhibit impaired function and characteristics of effector T cells. Further study showed that the expression of NF-κB c-Rel, a key mediator of effector T cell function, was significantly increased in inflam-Tregs. Mechanistic study revealed that elevated NF-κB c-Rel level in inflam-Tregs impaired Treg function through the promotion of inflammatory cytokine production and glycolysis. More importantly, we demonstrated that targeting NF-κB c-Rel was able to improve the immune suppressive function of inflam-Tregs in vitro and enhance the potential of them to suppress corneal transplantation rejection. Therefore, our current study identified NF-κB c-Rel as a key mediator of the conversion of Tregs into effector-like cells when under inflammatory environment.     

安达美在不同溶液中的稳定性研究

目的探讨安达美在不同浓度葡萄糖注射液及不同种类氨基酸注射液中的稳定性。方法分别测定10%葡萄糖注射液、5%葡萄糖注射液、3种氨基酸注射液的pH值,再分别测定安达美加入以上注射液中的pH值及变色现象,同时观察在上述溶液中添加安达美与电解质的先后顺序不同其pH值及变色情况。结果 10%葡萄糖注射液pH值平均3.46,5%葡萄糖注射液平均4.53;安达美加入葡萄糖注射液后溶液变色,pH值下降明显。3种氨基酸的平均pH值分别为5.50、6.70、6.61;安达美加入氨基酸注射液不变色,pH值变化较小。在氨基酸注射液中添加安达美与电解质的先后顺序不同未见明显颜色改变。结论安达美最好加入氨基酸注射液中,以避免其理化性质发生改变,保证配制后营养液的稳定性。     

雷米芬太尼控制性降压对颅脑手术患者血液动力学的影响

目的观察神经外科手术中雷米芬太尼控制性降压对血液动力学的影响。方法20例ASAⅠ~Ⅱ级择期行颅脑手术患者,采用全凭静脉复合麻醉,术中持续泵注雷米芬太尼行控制性降压,维持MAP 60~70 mmHg,观察降压前(T0)、降压达目标时(T1)、维持降压15 min(T2)、30 min(T3)、停降压15 min(T4)3、0 min(T5)6个时点的MAP、HR、心输出量(CO)、心脏指数(CI)、心室收缩加速度指数(ACI)、左心做功(LCW)、外周血管阻力(SVR)的变化。结果与T0比较,T1、T2、T3时点MAP、SVR、LCW均显著降低(P<0.01);HR亦明显减慢(P<0.05);CO、CI、ACI无明显降低。结论神经外科手术中行雷米芬太尼控制性降压安全、有效,具有对心功能影响小的优点。     

印象管理策略及其在护患沟通中的应用

患者对护理人员的看法直接影响到护患沟通的有效性.实施印象管理策略可对护患的有效沟通提供帮助,护理人员的印象管理策略有两种,一种是获得患者积极看待,包括完善自己、表现耐心和爱心、学会自我控制、自我展示、构建权威形象;一种避免患者消极看待,包括合理化理由、预告风险、承认错误,赔礼道歉.护理人员将印象管理策略运用于护患沟通中有助于改善护患关系,有助于使患者产生期望的行为模式,提高患者的依从性和满意度.     

Study of drug control over postoperative hemorrhage after selective caesarean section

Objective: To compare the efficacy and safety of carbetocin and oxytocin in the prevention of postpartum hemorrhage after selective caesarean section. Methods: Two hundred and sixteen pregnant women who were going to deliver by caesarean section were randomly divided into two paralleled and controlled groups, of which the oxytocin group(group A) contained 105 women and the carbetocin group(group B) 111 women. 20 IU of oxytocin was administrated during operation for both groups, 10 IU directly into uterus and 10 IU intravenously. When the operation was over, 20 IU of oxytocin was dripped for group A lasting for 8 hours, while for group B 100μg of carbetocin was immediately injected intravenously for 1 minute. Blood loss as well as hemoglobin alteration was measured for the comparison of the effect of the two drugs. Blood biochemical indicators were introduced for the evaluation of the safety of carbetocin including the function of liver and kidney and the fasting glucose.Results: No significant difference was revealed between the two groups concerning the postpartum hemorrhage within 24 hours, the postpartum hemorrhage incidence and the changes of hemoglobin. There was no significant difference in the change of liver and kidney function, fasting glucose and electrolyte changes as well.Conclusions: Carbetocin has a similar effect and safety of oxytocin in controlling the blood loss of selective caesarean section.     

严重继发性甲状旁腺功能亢进症临床分析

目的调查血液透析患者全段甲状旁腺激素（iPTH）、血清钙和血清磷的水平,了解严重的继发性甲状旁腺功能亢进症（SHPT）的发病情况。方法选择血液透析治疗超过1年,行3次以上iPTH、Ca和P检查的患者,以血iPTH〉800ng／ml为界定点,分析严重的SHPT发病率与年龄、性别、透析龄、原发病（糖尿病或非糖尿病）、血清钙和血清磷的关系。结果139例患者中有27例是严重的SHPT患者,发病率为19．4％（27／139）,它们拥有较长的透析龄（P〈0．05）;其糖尿病的发生率明显低于非糖尿病（P〈0．05）,而且存在明显的高磷血症和高钙磷乘积（P〈0．05）。Logistic回归分析显示,年龄、高钙磷乘积是严重的SHPT的独立危险因素（P〈0．05）。结论严重的SHPT的发病率与年龄、高钙磷乘积有关。因此,临床上必须早治疗,严格控制高磷血症。     

穿心莲内酯对TBHP诱导的椎间盘髓核细胞凋亡的作用及机制研究

目的：探讨穿心莲内酯（andrographolide,ANDRO）对叔丁基过氧化氢（tert-butyl hydroperoxide,TBHP）诱导的椎间盘髓核细胞（nucleus pulposus cells,NPC）凋亡的作用及其机制。方法：用TBHP诱导NPC建立细胞模型。将NPC分为5组：对照组、模型组（100μmol/L TBHP）、ANDRO低剂量组（100μmol/L TBHP+9μmol/L ANDRO）、ANDRO中剂量组（100μmol/L TBHP+18μmol/L ANDRO）和ANDRO高剂量组（100μmol/L TBHP+36μmol/L ANDRO）。流式细胞术检测细胞凋亡和活性氧（reactive oxygen species,ROS）水平,ELISA试剂盒检测氧化应激相关指标超氧化物歧化酶（superoxide dismutase,SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-PX）和过氧化氢酶（catalase,CAT）水平,JC-1探针法检测线粒体膜电位,Western blot检测动力相关蛋白1(dynam...     

糖尿病病人胰岛素泵应用及相关知识调查

[目的]探讨糖尿病病人应用胰岛素泵强化治疗的相关知识及需求情况.[方法]采用自行设计的问卷调查表,对60例糖尿病胰岛素泵强化治疗病人进行调查.[结果]病人对胰岛素泵了解甚少,相关知识缺乏,希望接受胰岛素泵知识教育.[结论]胰岛素泵应用现状存在较多问题,影响病人血糖的良好控制,应提高病人对胰岛素泵的认可程度和依从性,提高生活质量.