血清TSH和t-PINP/β-CTX比值对老年椎体骨折患者的诊断价值

目的 通过测定老年骨质疏松患者的促甲状腺素(thyroid stimulating hormone,TSH)水平及相关骨代谢生化标志物,研究其与椎体骨折的相关性.方法 选取2018年1月至2019年12月收治入院的年龄>60岁的符合骨质疏松与亚临床甲状腺功能紊乱诊断标准的老年骨科患者.依据是否合并椎体骨折将其分为无骨折...     

选择性血管内溶栓治疗急性周围动脉阻塞性疾病的临床应用

目的：探讨血管内溶栓治疗急性周围动脉阻塞性疾病的疗效及价值。方法：对14例血管阻塞性疾病进行国产尿激酶高剂量冲击疗法和短时间（平均5小时）局部灌注溶栓治疗。结果：14例急性血管阻塞性疾病完全或部分开通率达100％。无一例并发症。结论：急性周围动脉栓塞性疾病选择性血管溶栓治疗应是首选方法,注意掌握适应证及禁忌证,以及插管操作技巧。     

中药浓缩液坐浴对肛周脓肿术后创面愈合的影响

目的 观察中药浓缩液痔科洗剂坐浴对肛周脓肿一期根治术后创面愈合的临床疗效.方法 对198例住院患者随机分为两组,观察组103例采用中药浓缩液痔科洗剂坐浴,对照组95例采用1:5 000高锰酸钾(PP)溶液坐浴.观察两组患者术后创面的水肿、疼痛、渗血情况及创面愈合时间.结果 试验组疗效明显优于对照组,差异有统计学意义(P＜0.05).结论 中药浓缩液痔科洗剂坐浴中药坐浴可明显减轻术后切口疼痛、渗血,减轻水肿,缩短切口愈合时间.     

CircFAT1 sponges miR-375 to promote the expression of Yes-associated protein 1 in osteosarcoma cells

Background

There is an urgent need to identify new molecular targets for treatment of osteosarcoma. Circular RNAs are a class of endogenous RNAs that are extensively found in mammalian cells and exert critical functions in the regulation of gene expression, but in osteosarcoma the underlying molecular mechanism of circular RNAs remain poorly understood. Here we assessed the tumorigenesis properties of a circular RNA, circFAT1 in osteosarcoma.

Methods

The effects of circFAT1/miR-375/YAP1 was evaluated on human osteosarcoma cells growth, apoptosis, migration, invasion and tumorigenesis. Signaling pathways were analyzed by western blotting, qRT-PCR, fluorescence in situ hybridization, chromogenic in situ hybridization,RNA Binding Protein Immunoprecipitation and immunofluorescence. The consequence of circFAT1 short hairpin RNA combined or not with miR-375 sponge was evaluated in mice bearing 143B xenografts on tumor growth.

Results

In this study, we observed significant upregulation of circFAT1 originating from exon 2 of the FAT1 gene in human osteosarcoma tissues and cell lines. Inhibition of circFAT1 effectively prevented the migration, invasion, and tumorigenesis of osteosarcoma cells in vitro and repressed osteosarcoma growth in vivo. Mechanistic studies revealed that circFAT1 contains a binding site for the microRNA-375 (miR-375) and can abundantly sponge miR-375 to upregulate the expression of Yes-associated protein 1. Moreover, inhibition of miR-375 reversed attenuation of cell proliferation, migration, and invasion, which was induced by circFAT1 knockdown, and therefore promoted tumorigenesis.

Conclusions

Our findings demonstrate a novel function of circFAT1 in tumorigenesis and suggest a new therapeutic target for the treatment of osteosarcoma.

     

Phase-sensitive OCT imaging of multiple nanoparticle species using spectrally multiplexed single pulse photothermal excitation

We apply phase-sensitive optical coherence tomography to image multiple nanoparticle species with two excitation wavelengths matched to their distinct absorption peaks. Using different modulation frequencies, multiple species collocated within the sample can be distinguished. In addition, we characterize single-pulse excitation schemes as a method to minimize bulk heating of the sample. We demonstrate this new scheme with B-mode photothermal measurements of tissue phantoms.OCIS codes: (170.4500) Optical coherence tomography, (160.4236) Nanomaterials, (110.3000) Image quality assessment     

Detection of early colorectal cancer development in the azoxymethane rat carcinogenesis model with Fourier domain low coherence interferometry

Fourier domain low coherence interferometry (fLCI) is an emerging optical technique used to quantitatively assess cell nuclear morphology in tissue as a means of detecting early cancer development. In this work, we use the azoxymethane rat carcinogenesis model, a well characterized and established model for colon cancer research, to demonstrate the ability of fLCI to distinguish between normal and preneoplastic ex-vivo colon tissue. The results show highly statistically significant differences between the measured cell nuclear diameters of normal and azoxymethane-treated tissues, thus providing strong evidence that fLCI may be a powerful tool for non-invasive, quantitative detection of early changes associated with colorectal cancer development.     

荆州地区耐甲氧西林金黄色葡萄球菌的流行和耐药特征分析

目的 调查荆州地区社区相关性和医院相关性耐甲氧西林金黄色葡萄球菌(CA-MRSA和HA-MRSA)的流行和耐药特征.方法 连续收集2012年1月至2013年12月分离自荆州市中心医院门诊和住院患者送检样本的MRSA 159株.检测16种抗菌药物对所有菌株的最小抑菌浓度,采用多重PCR检测所有菌株的SCCmec分子型别,并分别以脉冲场凝胶电泳(PFGE)和耐药谱聚类分析对ICU的MRSA进行同源性分析.采用WHONET 5.6和SPSS 19.0软件对相关数据进行统计分析.结果 159株MRSA中,HA-MRSA 131株(82.4％),CA-MRSA 28株(17.6％).HA-MRSA和CA-MRSA在患者年龄、病区分布、来源样本类型、住院时间、抗感染治疗时间、感染类型和基础疾病的构成上差异有统计学意义(x2=19.103,31.372,59.756,71.703,54.153,59.756和54.232,P＜0.01).159株MRSA中未检出对万古霉素、利奈唑胺、替加环素和呋喃妥因耐药的菌株,但所有菌株对青霉素、头孢西丁和苯唑两林均耐药.HA-MRSA对莫西沙星、利福平、左氧氟沙星、环丙沙星和庆大霉素的耐药性高于CA-MRSA(x2=30.179,27.352,28.523,28.523和25.987,P＜0.01),但对红霉素和克林霉素的耐药性低于CA-MRSA(x2=13.106和11.743,P＜0.01).159株MRSA中,SCCmecⅡ型12株(7.5％),Ⅲ型113株(71.1％),Ⅳ型26株(16.4％),未能分型8株,CA-MRSA和HA-MRSA分别以Ⅳ(26/28,92.9％)型和Ⅲ型(113/131,86.3％)为主.ICU病区分离的49株HA-MRSA,经PFGE分型可分为6型,以Al(24株,49.0％)、A2亚型(9株,18.4％)和B型(9株,18.4％)为主;耐药谱聚类分析发现3群相关性很高的HA-MRSA,其对应的PFGE型别显示其分属于A1、A2亚型和B型.结论 荆州地区流行的MRSA以HA-MRSA为主,其与CA-MRSA在患者年龄、病区分布、感染类型以及耐药性等方面均显示出差异.HA-MRSA以SCCmecⅢ型为主,且在ICU病区存在HA-MRSA暴发流行.     

扩展经皮微波凝固肝癌组织范围的临床研究

 目的 探讨扩展经皮微波凝固疗法(PMCT)治疗空间的方法学。方法 运用自制HM-WIA型经皮微波肿瘤治疗仪,在彩超引导下对26个肝癌进行单电极微波凝固治疗36次。结果 单微波电极、单次凝固范围：长径4.52±0.77Cm,短径3.77±0.57cm;对直径2.5～3.0cm和直径3.0～4.0Cm的肝癌,一次覆盖率分别为100％和70％;对直径4.0cm以上的瘤体,优先凝固滋养血管区,再次PMCT的凝固体积平均为首次的2.36倍,最大短径为5.6cm。结论 该治疗系统有效扩大了单电极凝固治疗范围;采用中等功率(45、55W),单次凝固的范围于6～10min达到饱和;采用优先凝固瘤内滋养血管区策略,使血供减少,再次凝固范围倍增,使PMCT更适宜治疗较大肝癌。     

Structural basis for catalytic activation of protein Z-dependent protease inhibitor (ZPI) by protein Z

The anticoagulant serpin, protein Z-dependent protease inhibitor (ZPI), is catalytically activated by its cofactor, protein Z (PZ), to regulate the function of blood coagulation factor Xa on membrane surfaces. The X-ray structure of the ZPI-PZ complex has shown that PZ binds to a unique site on ZPI centered on helix G. In the present study, we show by Ala-scanning mutagenesis of the ZPI-binding interface, together with native PAGE and kinetic analyses of PZ binding to ZPI, that Tyr240 and Asp293 of ZPI are crucial hot spots for PZ binding. Complementary studies with protein Z-protein C chimeras show the importance of both pseudocatalytic and EGF2 domains of PZ for the critical ZPI interactions. To understand how PZ acts catalytically, we analyzed the interaction of reactive loop-cleaved ZPI (cZPI) with PZ and determined the cZPI X-ray structure. The cZPI structure revealed changes in helices A and G of the PZ-binding site relative to native ZPI that rationalized an observed 6-fold loss in PZ affinity and PZ catalytic action. These findings identify the key determinants of catalytic activation of ZPI by PZ and suggest novel strategies for ameliorating hemophilic states through drugs that disrupt the ZPI-PZ interaction.