In vitro activities of inulin fermentation products to HCT-116 cells enhanced by the cooperation between exogenous strains and adult faecal microbiota

Inulin was fermented by adult faecal microbiota and 10 exogenous strains for 24 or 48?h. The contents of acetate, propionate, butyrate and lactate were quantified in the fermented products, and the growth-inhibitory and apoptosis-inducing effects on a human colon cell line (HCT-116 cells) were assessed. Most of these strains increased contents of acetate, propionate and butyrate, and promoted lactate conversion. Correlation analysis suggested that butyrate and lactate in the fermentation products were positively and negatively correlated with the measured inhibition ratios (p?via inducing DNA fragmentation and increasing total apoptotic populations in the treated cells. Moreover, the fermentation products with higher growth-inhibitory activities demonstrated the increased apoptosis-inducing properties. In conclusion, these strains could cooperate with adult faecal microbiota to confer inulin fermentation products with higher anti-colon cancer activity.     

直丝弓矫治技术用于安氏骨性Ⅱ类错畸形治疗前后面型的改变

目的探讨直丝弓矫治技术对安氏Ⅱ类骨性错畸形患者骨面型的改变情况。方法在矫治前拍摄的头颅定位侧位片上,筛选出8例安氏Ⅱ类骨性错畸形患者,应用直丝弓矫治技术进行矫治,矫治结束后,在同样条件下再拍摄头颅定位侧位片,行头影测量分析,与治疗前数值进行比较,观察骨面型的改变情况。结果直丝弓矫治对安氏Ⅱ类骨性错畸形患者的面型略有改变,但患者的深覆及深覆盖的纠正更多是由下前牙唇侧倾斜来代偿完成。结论安氏Ⅱ类骨性错畸形患者在恒牙期早期或恒牙期直接采用直丝弓矫治技术进行矫治,对牙齿排列和咬合关系调整虽然有一定的治疗效果,但是对侧面型的改善程度有限,不能达到理想的矫治效果。     

玻璃离子水门汀与银汞粘接的剪切力测试

探讨了玻璃离子水门汀增强银汞充填体与牙体之间的粘结作用。取新鲜离体人磨牙制成3mm厚的牙块,在其中央制备直径3mm的圆柱形洞,分别以玻璃离子、银汞粘结剂涂于洞壁,再充填银汞合金,另设空白对照组。两周后测各组的剪切力并作统计学分析。结果表明:玻璃离子与银汞粘结剂组的剪切力比空白对照组大,并有显著差异;说明玻璃离子与银汞粘结剂均有相似的增强银汞修复体与牙体之间粘结力的作用。     

Attachment of gold nanoparticles to glassy carbon electrode and its application for the voltammetric resolution of ascorbic acid and dopamine

Gold nanoparticles have been attached on glassy carbon electrode surface through sulfhydryl-terminated monolayer and the gold nanoparticles-immobilized glassy carbon electrodes have been applied to the electrocatalytic oxidation of ascorbic acid, reducing the overpotential by about 200 mV with obviously increased current response. Due to its strong electrocatalytic activity towards ascorbic acid, the gold nanoparticles modified electrode can resolve the overlapped voltammetric waves of ascorbic acid and dopamine into two well-defined voltammetric peaks with peak-to-peak separation in potentials of about 300 mV. This can be used to allow the selective determination of ascorbic acid in the presence of dopamine. The catalytic current obtained from differential pulse voltammetry is linearly dependent on ascorbic acid concentration over the range of 6.5 × 10^?6 to 1.45 × 10^?4 M with correlation coefficient of 0.998 in the presence of dopamine. The detection limit (3σ) for AA was found to be 2.8 × 10^?6 M. The simultaneous determination of ascorbic acid and dopamine in their binary mixture has also been investigated. The modified electrode shows good selectivity, stability and anti-fouling properties. The proposed methods have been used for the selective determination of ascorbic acid in the presence of dopamine and for the simultaneous determination of both them in their mixtures with satisfactory results.     

用新型组合式矫治装置早期矫治安氏Ⅱ~1错

目的:用新型组合式矫治装置治疗替牙期及恒牙初期安氏Ⅱ1错牙合。方法:采用由口外弓、下颌唇挡及上颌斜面导板组成的矫治装置,治疗替牙期及恒牙初期安氏Ⅱ1错牙合66例(男3 0例,女3 6例) ,治疗前后进行X线头影测量分析。结果:66例患者的覆牙合、覆盖及颌关系调整时间为3～13个月,平均7.6个月。治疗后覆牙合平均减小2 .5mm ,覆盖减小4.3mm ,SNB角增加1.8°,ANB角减小1.9°,U 1 NA角减小8.8°,L1 NB角增加6.4°,IMPA角增加5 .7°,Z角增加4.5°,Wits值减小1.6,ANS Me增加4.0mm。结论:该组合式矫治装置对替牙期及恒牙初期安氏Ⅱ1错牙合具有良好的矫治效果。     

义齿修复工作模型超硬石膏用量分类初探

目的:通过测量义齿修复工作模型重量,计算各模型所含超硬石膏粉的用量,再按其粉用量,对义齿修复工作模型进行统计学分类.以期依据分类指导临床灌注模型,控制超硬石膏粉的用量和标准水粉比例,达到提高模型质量、节约材料的目的.方法:随机选取临床小号、中号、大号3种托盘制取的修复工作模型311例,计算出工作模型所需超硬石膏粉的用量,实验数据采用SPSS 13.0软件包进行分析,统计各型号托盘印模所需超硬石膏粉用量的均数值进行分类,并临床试用验证.结果:义齿修复工作模型按其超硬石膏的用量可分为3类,小号模型所需超硬石膏粉≤45g,中号为46～55g,大号为56～65g.验证样本均在超硬石膏粉用量分类范围之内.结论:义齿修复工作模型所需超硬石膏粉用量与托盘大小、牙弓大小基本对应,依此作为工作模型的分类指标和用量标准较为合理,用于临床可以适当计量超硬石膏粉用量和按标准水粉比例灌注工作模型,提高模型质量,节约材料.     

产黑色素普氏菌DNA探针的制备及鉴定

本研究旨在应用DNA探针杂交技术快速检测产黑色素普氏菌、利用产黑色素普氏菌经酚抽取全染色体DNA，并以缺口转移法标记32P，制备探针。结果证实，该探针能够检测到膜上2×104的纯菌；当产黑色素普氏菌和中间普氏菌以不同比例混合点膜时，该探针能够检测到占混合菌群17％的目的菌；与口腔感染常见菌株杂交，未见交叉反应。     

四种光固化复合树脂修复物边缘微渗漏的体外试验研究

目的:比较4种光固化充填材料修复体边缘微渗漏.方法:在新鲜拔除的40个人前磨牙的颊侧牙颈部制洞,将牙齿分为4组,每组10个.分别充填4种修复材料Restorative Z-350、Z-100(3M),Chafisma(Dentsply),Durafill(Kulzer)经冷热交替试验(4～60℃,循环600次),采用10 g/L碱性品红染料渗入法,在体视显微镜下观察修复体与牙体洞壁间边缘微渗漏.结果:4种材料中3M-Z350微渗漏最小,Durafill最大,DurafiU与其他3组相比有显著性差异(P＜0.05);3M-Z100和Chrisma无显著性差异(P＞0.05);3MZ350与其他3组相比有显著性差异(P＜0.01).结论:3M-Z350可能是临床修复牙体缺损的较好材料.