c-myc mRNA在成釉细胞瘤中的表达

目的:研究成釉细胞瘤(AB)和牙源性角化囊肿(OKC)中c-mycmRNA的表达,探讨c-myc在AB和OKC中的发生、发展及其生物学意义。方法:使用原位杂交法检测54例AB、16例OKC和7例口腔正常黏膜(NOM)组织中c-mycmRNA的表达,并将AB按原发、复发、恶变分组,结果使用χ2检验进行统计分析。结果:AB、OKC及NOM组织中c-mycmRNA的阳性表达率分别为81.5%(44/54)、75.0%(12/16)和14.3%(1/7),3组比较有显著性差异(χ2=15.488,P<0.05)。原发组AB中c-mycmRNA的阳性表达率为71.0%,复发组为94.7%,恶变组为100.0%,伴随原发、复发、恶变,差异有显著性(χ2=16.912,P﹤0.05)。结论:c-myc表达在AB的发生、发展中有重要作用;c-mycmRNA的表达与AB的临床生物学行为有关,伴随其生物学行为变化,c-mycmRNA表达增强;提示c-myc有可能成为评价预后的有效指标。     

义齿PMMA基托表面粗糙度影响的实验研究

目的：研究技工操作因素对义齿树脂基托表面粗糙度的影响。方法：在不同粉液比、充填时期、聚合温度、混合方式以及出盒温度成型的上颌半口义齿基托上选取1.5cm^2范围，按照统一的打磨顺序，由粗到细打磨抛光完成，浸入冷水中，7天后进行粗糙度测试，所得数据经t检验统计处理。结果：在单体含量降低组、丝状期充填组．热处理时直接放入70℃水中维持9h组与对照组相比，均存在显著差异（P〈0．05）。结论：影响PMMA树脂表面粗糙度的制作工艺因素有单体含量降低、丝状期充填和未经100℃高温热处理。     

Mechanism of uptake and retention of F-18 BMS-747 158-02 in cardiomyocytes: a novel PET myocardial imaging agent

Background BMS-747158-02 is a novel fluorine 18-labeled pyridazinone derivative designed for cardiac imaging. The uptake and retention mechanisms of F-18 BMS-747158-02 in cardiac myocytes were studied in vitro, and the biodistribution of F-18 BMS-747158-02 was studied in vivo in mice. Methods and Results Fluorine 19 BMS-747158-01 inhibited mitochondrial complex I (MC-I) in bovine heart submitochondrial particles with an IC₅₀ of 16.6±3 nmol/L that was comparable to the reference inhibitors of MC-1, rotenone, pyridaben, and deguelin (IC₅₀ of 18.2±6.7 nmol/L, 19.8±2.6 nmol/L, and 23.1±1.5 nmol/L, respectively). F-18 BMS-747158-02 had high uptake in monolayers of neonatal rat cardiomyocytes (10.3%±0.7% of incubated drug at 60 minutes) that was inhibited by 200 nmol/L of rotenone (91%±2%) and deguelin (89%±3%). In contrast, an inactive pyridaben analog, P-0 (IC₅₀ value>4 μmol/L in MC-1 assay), did not inhibit the binding of F-18 BMS-747158-02 in cardiomyocytes. Uptake and washout kinetics for F-18 BMS-747158-02 in rat cardiomyocytes indicated that the time to half-maximal (t_1/2) uptake was very rapid (approximately 35 seconds), and washout t_1/2 for efflux of F-18 BMS-747158-02 was greater than 120 minutes. In vivo biodistribution studies in mice showed that F-18 BMS-747158-02 had substatial myocardial uptake (9.5%±0.5% of injected dose per gram) at 60 minutes and heart-to-lung and heart-to-liver ratios of 14.1±2.5 and 8.3±0.5, respectively. Positron emission tomography imaging in the mouse allowed clear cardiac visualization and demonstrated sustained myocardial uptake through 55 minutes. Conclusions F-18 BMS-747158-02 is a novel positron emission tomography cardiac tracer targeting MC-I in cardiomyocytes with rapid uptake and slow washout. These characteristics allow fast and sustained accumulation in the heart.     

护理业务流程再造现状分析及对策

目的了解护士对护理业务流程再造的知晓度及需求,找出流程再造存在的问题,针对存在问题提出相应的对策。方法采用问卷调查方法,调查广东省16所县级以上医院287名护士对护理业务流程再造的认识情况。结果护士对护理流程再造的知晓现状与临床状况不尽人意;护士认为进行护理流程优化再造是必要的和重要的;现有的护理流程存在的问题是流程流于形式、不完善及实用性不强。结论要进行护理流程再造,必须转变观念,加强在职培训,设计精简、方便、实用的护理流程。     

原发性恶性骨肿瘤的治疗进展

1简介在人类全部肿瘤中,原发骨肿瘤相对少见,因此只有少数肿瘤中心可收治到数量比较多的病例。近年来在骨肿瘤的诊断和治疗领域已经取得了很大的进步。放射医师与病理医师和临床医师紧密地合作,应用大量先进的技术使我们对这种恶性疾病有了更加深入的了解,从而可以更好地对病人     

输尿管口囊肿的微创手术治疗

目的评价输尿管口囊肿的微创手术治疗方法及其疗效。方法回顾性分析25例行经尿道电切（TURU）手术治疗输尿管口囊肿患者资料。男9例，女16例；年龄18—61岁，平均38岁；单侧18例，双侧7例；单纯输尿管囊肿22例，异位输尿管囊肿3例。结果25例患者手术一次成功22例，手术平均时间15min，术后平均住院3d。术后定期B超、膀胱造影随诊，随诊时间1—9年，3例异位输尿管囊肿合并重复肾、输尿管畸形患者存在术后膀胱输尿管返流，2例再次行开放手术后痊愈。结论TURU是治疗成人输尿管口囊肿的有效方法；当合并存在重复肾输尿管畸形或术后膀胱输尿管返流时，应采用输尿管膀胱抗返流手术。     

腹腔镜与开放性肾囊肿去顶术的临床对照分析

目的评价腹腔镜与开放性肾囊肿去顶术的临床疗效。方法回顾性分析采用经后腹腔镜和经腹腔镜行肾囊肿去顶术21例，其中经腹腔5例，经后腹腔16例，与开放性手术16例比较。结果手术时间，术中平均出血量，术后下床活动时间，术后住院天数，术后并发症腹腔镜组均优于开放手术组，住院费用腹腔镜组略高于开放性手术组。结论腹腔镜肾囊肿去顶术治疗单纯性肾囊肿安全、有效。应成为治疗单纯性肾囊肿的首选方法。     

APTT测定值和APTT测定值比率的临床应用价值探讨

目的;探讨APIT测定值(秒)和APIT测定值比率中哪一种是更稳定、更可靠的临床应用指标。方法:将同一标本采用法国STA-Compat全自动血凝仪以及两种手工法同时测定,比较同组标本不同方法的APTT测定值(秒)和APTT测定值比率。结果:手工法(一)与法国STA-Compact全自动血凝仅比较,如APTT值以秒表示,对照组、口服抗凝剂组及肝病组病人同组标本不同方法之间存在显著性差异(P<0.01,P0.10,P>0.50,P>0.05);手工法(二)与法国STA-Compact全自动血凝仪在对照组、口服抗凝剂组及白血病组的比较中,也获得类似的结果。结论:将APTT测定值(秒)转化为病人与对照APTT测定值比率报告,使不同方法结果间更具可比性。在APTT报告的标准化方面,建议将APTT和APTT测定值比率同时报告。     

Angiopoietin1/Tie2 and VEGF/Flk1 induced by MSC treatment amplifies angiogenesis and vascular stabilization after stroke.

Bone marrow stromal cells (MSCs) increase vascular endothelial growth factor (VEGF) expression and promote angiogenesis after stroke. Angiopoietin-1 (Ang1) and its receptor Tie2 mediate vascular integrity and angiogenesis as does VEGF and its receptors. In this study, we tested whether MSC treatment of stroke increases Ang1/Tie2 expression, and whether Ang1/Tie2 with VEGF/ vascular endothelial growth factor receptor 2 (VEGFR2) (Flk1), in combination, induced by MSCs enhances angiogenesis and vascular integrity. Male Wistar rats were subjected to middle cerebral artery occlusion (MCAo) and treated with or without MSCs. Marrow stromal cell treatment significantly decreased blood-brain barrier (BBB) leakage and increased Ang1, Tie2, and occludin (a tight junction protein) expression in the ischemic border compared with MCAo control. To further test the mechanisms of MSC-induced angiogenesis and vascular stabilization, cocultures of MSCs with mouse brain endothelial cells (MBECs) or astrocytes were performed. Supernatant derived from MSCs cocultured with MBECs significantly increased MBEC expression of Ang1/Tie2 and Flk1 compared with MBEC alone. Marrow stromal cells cocultured with astrocytes also significantly increased astrocyte VEGF and Ang1/Tie2 expression compared with astrocyte culture alone. Conditioned media from MSCs alone, and media from cocultures of MSCs with astrocytes or MBECs, all significantly increased capillary tube-like formation of MBEC compared with control Dulbecco's modified Eagle's medium media. Inhibition of Flk1 and/or Ang1 significantly decreased MSC-induced MBEC tube formation. Knockdown of Tie2 expression in MBECs significantly inhibited MSC-induced tube formation. Our data indicate MSC treatment of stroke promotes angiogenesis and vascular stabilization, which is at least partially mediated by VEGF/Flk1 and Ang1/Tie2.     

质子磁共振波谱对创伤后应激障碍患者海马神经元异常的诊断价值

目的 探讨质子磁共振波谱（1HMRS）在诊断创伤后应激障碍（post traumatic stress disorder，PTSD）患者海马神经元异常中的价值。方法 对19例PTSD患者及19例健康对照组行常规MRI和1HMRS检查。结果 PTSD患者MRI检查未出现信号的异常。19例患者两侧海马均显示NAA／Cr明显减低，与健康对照组相比，差异有统计学意义（P〈0．01）；而患者两侧海马的Cho／Cr与健康对照组相比，差异无统计学意义（P〉0．05）。结论 1HMRS揭示了PTSD时海马存在着神经元的丢失或功能紊乱，而这些改变是构成PTSD的神经生物学基础之一。