分泌性中耳炎对内耳听觉功能的影响

为了解分泌性中耳炎(OME)对内耳听觉功能的影响,对OME220例、304耳分别进行纯音测听及声阻抗测定.结果表明,部分OME患者骨导听阈升高,除造成传音性耳聋外,合并感音性耳聋(SNHL)为31.8%,并随着病程延长,SNHL发病率增高.提示应积极有效地治疗本病,应用扩血管及营养神经药物可防治SNHL的发生.     

自身免疫性心肌炎发病机制和临床意义的研究进展

自身免疫受遗传、免疫、激素和环境等因素影响,病毒感染可能会触发自身免疫.在各种类型心脏病的发病过程中自身免疫可能扮演一个重要角色,被定义为由心肌功能受损引起的心肌炎症反应,是导致扩张型心肌病最常见的病因.心肌炎可以由感染和非感染因素引起,非感染因素包括伴随心肌炎的自身免性疾病.为了更好地了解自身免疫性心肌炎,该文就自身免疫性心肌炎的发病机制和临床意义作一综述.     

Toll‐like receptors elicit different recruitment kinetics of monocytes and neutrophils in mouse acute inflammation

Leukocyte recruitment is an important process in combating pathogens. The largest class of circulating leukocytes are neutrophils, which rapidly invade inflamed tissue, followed by inflammatory Ly6C⁺ monocytes. Ly6C^low monocytes patrol the endothelial wall routinely in the steady state. We recently reported early luminal recruitment of Ly6C^low monocytes, which preceded and orchestrated neutrophil arrival and extravasation in response to TLR7/8‐mediated vascular inflammation. Here we dissected the kinetics of recruitment of monocytes and neutrophils and examined the dynamics of Ly6C^low monocytes in response to several other Toll‐like receptor (TLR) agonists, using intravital confocal microscopy. We observed two types of kinetics in mesenteric veins. TLR2, TLR5 and TLR9 agonists caused early monocyte and neutrophil influx whereas TLR3 and TLR4 agonists rapidly recruited neutrophils and caused Ly6C^low monocytes to arrive at low levels later on. All TLR agonists, except TLR9, led Ly6C^low monocytes to meticulously patrol the vascular wall. Finally, these monocytes released pro‐inflammatory cytokines and chemokines implicated in neutrophil recruitment in response to TLR2, TLR4, and TLR9 stimulation but not to TLR3 and TLR5 agonists. These results refine our understanding of the early events in the leukocyte recruitment cascade, including the patrolling behavior of Ly6C^low monocytes, in TLR‐mediated acute vascular inflammation.     

尼莫地平对脑缺血再灌注小鼠保护机制的研究

目的 :观察尼莫地平对小鼠脑缺血再灌注损伤的保护作用。方法 :将 6 3只昆明种小鼠分为假手术组、手术组和尼莫地平组 ,后两组结扎双侧颈总动脉 ,阻断血流 30min后恢复血流灌注 ,分别给予尼莫地平治疗和生理盐水对照。检测各组手术前及手术后各时间段血小板选择素CD6 2 p、红细胞内钙(IECa2 + )、红细胞变形指数 (DI)和全血粘度 (WBV) ,并观察脑组织形态学改变。结果 :尼莫地平组术后第 1天和第 2天CD6 2 p、IECa2 + 明显高于假手术组(P <0 .0 5) ;但随着治疗天数的增加 ,CD6 2 p、IECa2 + 下降至假手术组水平。脑组织形态学病理改变以手术组较重 ,其神经细胞数量明显减少 ,残存的细胞大部分肿胀、固缩或者变性、崩解。尼莫地平组仅见少数散在神经细胞肿胀、固缩、变性。结论 :尼莫地平治疗对缺血再灌注小鼠脑有保护作用 ,有利于脑血循环、脑功能的恢复。     

家庭暴力施暴者的心理健康状况

目的 探讨家庭男性躯体施暴者的心理健康状况,为进一步干预家庭暴力提供帮助.方法 采用病例时照研究,对156例社区家庭男性躯体施暴者和44例社区正常对照组用社会人口学指标、症状自评量表进行评定.结果 一般资料显示,家庭暴力男性躯体施暴者较对照组有更多的低教育水平者;更多的嗜烟者,且每天抽烟量大;且他们的躯体化、人际关系敏感、抑郁、敌对和偏执分值显著高于对照组(P＜0.05).结论 家庭暴力男性躯体施暴者有较多的低教育水平者和有较多的心理问题.     

小牛和大鼠肝生长刺激因子的提取及其对大鼠肝部分切除后肝细胞增殖的影响

从新生小牛肝及大鼠肝大部切除后的肝中用酒精反复沉淀和超滤方法提取肝生长刺激因子(HSS),利用32%肝部分切除后30小时的大鼠为检测模型研究HSS对大鼠再生肝细胞增殖的影响,结果发现,牛和鼠HSS均可使再生肝细胞分裂指数提高2～3倍,提示HSS可以促进肝细胞的分裂。牛HGS的剂量增加,其促肝细胞分裂的作用也随之增强。采用超滤法证明,HSS活性成分的分子量低于50KD。     

miR-21靶向PDCD4调控非小细胞肺癌A549细胞的增殖与迁移

目的:探讨miR-21靶向程序性细胞死亡因子4(programmed cell death factor4,PDCD4)对非小细胞肺癌(non-smallcell lung cancer,NSCLC)A549细胞增殖和迁移的影响及其作用机制.方法:采用脂质体转染技术分别将miR-21 mimics、miR-21inhi...     

A novel microdeletion upstream of HOXD13 in a Chinese family with synpolydactyly

Synpolydactyly (SPD) is a digital malformation with the typical clinical phenotype of the webbing of 3/4 fingers and/or 4/5 toes, and combined with polydactyly. In this study, we investigated a Chinese family with SPD and genetic analysis found that all of the affected individuals in the family carry a heterozygous 11,451 bp microdeletion at chr2:176933872-176945322 (GRCh37), which is located upstream of HOXD13 gene, the known disease gene for SPD1. All the affected individuals in the family carry the heterozygous deletion variant, and the variant co-segregated with SPD in the family. Thus, we speculate that the 11,451 bp microdeletion is the disease-causing variant in the family. To date, the microdeletion associating with SPD1 which we identified is the smallest deletion upstream of the HOXD13 gene and not altering the sequence of the HOXD13 gene.     

基于CiteSpace的针灸治疗带状疱疹的研究图谱分析＊

目的 通过探索针灸治疗带状疱疹相关研究，总结针灸治疗带状疱疹的研究脉络、热点和发展趋势。方法 检索中国知网（CNKI）1957年1月1日至2019年4月16日针灸治疗带状疱疹的相关文献，利用CiteSpace软件生成相关文献的作者、研究机构和关键词的共现图谱，并进行分析。结果 共有670篇文献被纳入，其中共有64位作者被纳入，黄国付、张红星、林国华和杨运宽等4位作者被引频次≥5。共有37所机构被纳入，共有5所机构被引频次≥5。共有98个关键词被纳入，带状疤疹、针灸治疗、缠腰火丹、带状疱疹、针灸疗法、疱疹、围刺等7个关键词出现爆发点，爆发点时间最近的是“围刺”，持续时间为2009-2012年。中介中心值最高的为“带状疱疹后遗神经痛”的0.3。结论 针灸治疗带状疱疹的研究热点主要在带状疱疹、针灸和带状疱疹后遗神经痛，热点趋向带状疱疹后遗神经痛。该方向的深入研究需要影响力大的作者之间进行沟通交流，加强多中心的合作，完善相关的基础研究。