中药黄酮类的研究 Ⅷ.野菊花成分的研究(第一报)

我們所用的野菊花产于上海市郊区奉賢县,經本所鉴定为Chrysanthemum indicumL.,系菊科野生多年生草本植物,其药用部分为干燥的头状花序。野菊花收載于《本草綱目》,为調中、止洩破血、治痈肿疗毒、瘰癧眼瘜之用。最近用于治疗高血压。在日本民間,用花部揮发油治腹痛、創伤。关于其化学成分,文献記載有花青素(chrysanthemin)、木犀黃酮甙(luteolinglucoside)和揮发油等成分。我們把乙醇提取液浓縮后的黃色析出物,用乙醚、氯仿洗后,再用吡啶/水(1:1)复結     

国产甘草成分含量测定的研究

1.本文简述了前人分析甘草成分所用的方法,並對Houseman氏及Fahmy氏所用方法的主要步驟進行了比較研究,改進了他們的操作方法. 2.為了便利更完全地分離甘草根中所含的甘草酸,我們進行了下列修改:(1)將Houseman氏沉澱甘草酸的時間由2.5小時延長至24小時;(9)將Houseman氏沉澱甘草酸時所用的硫酸濃度由0.3%提高至1.4%;(3)將Fahmy氏沉澱甘草酸時的温度由常温降低至6—7C°. 4.由於非林氏試液很不穩定,應用、貯存均感不便,故採用班乃第氏定量試液.用轉化後的糖液滴定一定量的班乃第定量試液,以测定總還原糖量. 6.本文報告了對五種国產甘草成分分析的結果;水溶物26.17—35.59%,甘草酸5.49—10.04%,總還原糖4.70—10.97%,澱粉及膠質4.17—5.92%,水分8.04—8.93%,灰分3.06—4.24%,酸不溶性灰分0.25—0.65%.並證明上述樣品完全符合於中、蘇、英、日、美各国藥典規定的甘草品質標準.     

Setting up a clinical audit of paediatric morbidity in Hong Kong: Some early experiences

Abstract A method of clinical audit was devised to document paediatric morbidity patterns at the Prince of Wales Hospital, a new 1428 bed general hospital serving the eastern part of the Hong Kong New Territories (with a current population of about 750 000). The applicability of the method is illustrated by considering morbidity in 1654 children aged from 1 month to 12 years admitted to the hospital over a 13 month period in 1984–85.     

定点突变法显示在高龄SHR大鼠心脏组织中KCNE2第98位氨基酸可能存在磷酸化修饰

目的:探究在高龄SHR大鼠心室膜蛋白中,KCNE2蛋白是否发生了磷酸化修饰。方法:用碱性磷酸酶处理高龄SHR大鼠心室膜蛋白,以抗KCNE2的抗体(Ab2)为一抗进行免疫印迹;将在蛋白离体系统中翻译出的含c-myc的KCNE2融合蛋白经碱性磷酸酶处理后,分别以Ab2和抗c-myc的抗体为一抗的免疫印迹;以及一系列的c-myc-hKCNE2的单点(KCNE2Y96F和KCNE2S98A)和双点突变(KCNE2Y96F/S98A)实验。结果:高龄SHR大鼠心室膜蛋白样品经碱性磷酸酶处理后,极大地减弱了Ab2对KCNE2蛋白的识别能力;另外,蛋白离体系统中翻译出的含c-myc的KCNE2融合蛋白经碱性磷酸酶处理后,虽然极大地减弱了Ab2对其融合蛋白的识别能力,却没有影响抗c-myc抗体对KCNE2融合蛋白的识别能力。定点突变的结果显示,虽然以抗c-myc抗体为一抗的免疫印迹结果显示,蛋白质离体翻译系统成功地翻译出了单突变子KCNE2S96A及双突变子KCNE2Y96F/S98A蛋白,但不论样品被碱性磷酸酶处理与否,Ab2在这些样品中均检测出很弱的KCNE2蛋白免疫条带。结论:在高龄SHR大鼠心室组织中,KCNE2第98位丝氨酸可能发生了磷酸化修饰。然而该位点的磷酸化修饰是否会通过影响心脏的电生理,而在SHR大鼠心脏病变过程中起着一定的作用,有待于从电生理的水平上进一步研究。     

Vectorcardiographic criteria for acute right ventricular infarction

Based on serial vectorcardiographic and cardiac scintigraphicstudies of 62 patients with acute myocardial infarction, wepropose vectorcardiographic criteria for the diagnosis of acuteright ventricular infarction. These criteria are: (1) the directionof the maximal spatial ST vector points either to the right-anterior-inferioror to the right-posterior–inferior octant, and (2) themagnitude of the projection of the maximal spatial ST vectoris 0.15 mV in the horizontal plane. By using these criteriacorrelated with scintigraphic results, 92% sensitivity was achievedtogether with 98% specificity; the Kappa statistic was 0.90.In patients with acute inferior and right ventricular infarction,the serial maximal spatial ST vector swung to-and-fro like a‘tug of war’ between right-anterior-inferior andright-posterior-inferior octants during the acute stage. Inpatients with acute inferior-posterior and right ventricularinfarction, the serial maximal spatial ST vector pointed tothe right-posterior-inferior octant during the whole courseof the acute stage. Failure to recognize this electrical phenomenonmay make the clinician inaccurate when judging the clinicalcourse of acute right ventricular infarction or over-estimatethe result of therapeutic intervention.     

下颌骨骨密度与颅面形态关系的研究

目的：探索不同垂直面型与下颌骨骨密度之间的关系。方法：应用双能骨密度吸收仪测量３ 组不同垂直面型（高角组、正常组和低角组） 样本的下颌骨骨密度。结果：面部基本对称的个体其下颌骨左右两侧的骨矿含量和骨密度分布基本对称,３ 个不同垂直面型组之间骨矿含量和骨密度差异有极显著性。结论：下颌骨骨密度与颅面垂直形态之间存在着密切的关系;双能骨密度吸收测量仪为下颌骨骨密度的定量测定提供了精确、简便而实用手段。     

马兜铃属植物的化学(Ⅰ)土青木香成分之分离

最近几年来,在寻找治疗高血压药物时,经上海市公费医疗第五门珍部等单位临床试验,发现土青木香对于治疗高血压有很好效果,据称其疗效接近于蘿芙木(Rauwolfia sp.)制剂,而毒性则较蘿芙木为小。按土青木香系Aristolochia debilis, S. et Z. 的根,是马兜铃科马兜铃属植物,因此我们从土青木香开始,为了作较有系统的研究,扩大到马兜铃属同属植物的化学研究。     

Effect of androgen withdrawal on activation of ERKs and expression of cell cycle regulation molecules in human prostate carcinoma cells

Objective: To explore the possible mechanisms of growth regression of human androgen dependent prostate carcinoma cells caused by androgen withdrawal. Methods: After 24 h of treatment with 1 × 10-9 mol/L dihydrotestosterone (DHT), the expression of phosphorylated ERK proteins and cell cycle regulation molecules including CDK2, CDK4, CDK6 and P27kip1 in human androgen dependent prostate carcinoma cell line LNCaP was measured by Western blot analysis 0 h, 8 h and 24 h of after androgen withdrawal. Human androgen independent prostate carcinoma cell line PC-3 was also examined as control. Results: Down-regulation of phosphorylated ERK, CDK2, CDK4 and CDK6 and up-regulation of P27kip1 were found initially in LNCaP cell line 8 h after androgen withdrawal. The levels of phosphorylated ERK and CDKs decreased continuously and reached the lowest after 24 h, while continuous elevation of P27kip1 was detected thereafter to 24 h. No expression change of phosphorylated ERK, CDKs and P27kip1 were detected in PC-3 cel