后腹腔镜与经腰背途径输尿管切开取石手术疗效比较

目的 对后腹腔镜和经腰背部途径治疗输尿管上段结石的疗效指标进行比较,探讨两种术式的技术要点.方法 收治98例上尿路结石病例,其中42例行后腹腔镜输尿管切开取石术,56例行经腰背部直切口输尿管切开取石术.腹腔镜术式采用气管插管全麻,经腰背部途径采用腰硬联合麻.两组术中均留置双J管.结果 98例手术全部成功,两组术中均无需输血.两组在手术时间、术中出血及住院时间有统计学差异,术后复查未见结石残留,随访无并发症发生.结论 对输尿管上段结石的治疗,后腹腔镜输尿管切开取石术微创、安全、有效,较经腰背部途径术中出血少、术后恢复快.但它需要较长的手术时间和一定的腹腔镜外科训练.     

静吸复合全麻用于经DSA治疗颅内动脉瘤患者的麻醉

现将本院2006年1月-2006年12月54例经DSA全脑造影及栓塞术的麻醉处理总结如下。 1资料与方法 1．1一般资料 本组54例患者中，男29例，女25例，年龄11-70岁，ASAⅡ-Ⅲ级，急诊11例。术前昏迷5例，术中动脉瘤破裂2例。     

单细胞凝胶电泳技术在生殖细胞DNA损伤检测中的应用

单细胞凝胶电泳技术(SCGE)是一种快速检测单个细胞DNA损伤的实验技术,在生殖细胞DNA损伤的检测中广泛应用。本综述系统介绍了SCGE在睾丸生精细胞、支持细胞、间质细胞、卵巢细胞以及卵母细胞等生殖细胞DNA损伤检测中的应用现状,并对SCGE在生殖毒性检测中的发展提出了展望。     

噻吗洛尔与普萘洛尔治疗高血压病与心绞痛的疗效比较

劳累性心绞痛38例采用噻吗洛尔2.5-5mg, tid,共4wk;另12例采用普萘洛尔10-20mg, tid,共4wk。结果:前者显效率为79％,显著高于后者42％(P<0.05)。高血压病(Ⅰ,Ⅱ期)37例采用噻吗洛尔治疗,方法同上;另13例采用普萘洛尔20-40mg, tid,疗程亦4wk。结果:2组均有显著降压疗效(P<0.01),噻吗洛尔治疗后1h即获显效,普萘洛尔须24h才获显效。噻吗洛尔7％(5/75)、普萘洛尔4％(1/25)治疗后发生窦性心动过缓,前者被迫停药,后者未停药。     

QT间期离散度的软件实现方法

本文着重从软件方面介绍了QT间期离散度的实现方法,包括心电信号的采集、显示、数字信号处理、关键点的识别等,并且给出了具体的实现方法、部分关键源程序及程序流程图.     

肌电引导治疗仪的设计

介绍一种操作简单、成本较低、便于医生确定靶肌肉的肌电引导治疗仪.该仪器经过临床初步试验,效果良好.     

直视下经尿道内切开术治疗尿道狭窄

目的：探讨直视下经尿道内次切开术治疗尿道狭窄的有效性和安全性。方法：总结直视下经尿道内切开术治疗68例尿道狭窄和闭锁患者的疗效和经验，63例1次手术成功；3例行2次、2例行3次成功。结果：68例中，57例术后随访3～71个月，平均28．3个月，43例(75％)均排尿通畅；2例暂时性尿失禁者分别于术后3～6月内恢复。结论：直视下经尿道内切开术创伤小，并发症少，疗效确切，是尿道狭窄和闭锁的首选治疗方法。     

西洋参茎叶皂甙单体Rb_3对大鼠血流动力学及单钙通道活动的影响

单体皂甙Ｒｂ３自西洋参茎叶皂甙中提取。Ｒｂ３３０ｍｇ·ｋｇ－１可使麻醉大鼠在体心脏的ＭＡＰ，±ｄＰ／ｄｔｍａｘ和ＬＶＳＰ减少。用斑片钳的连细胞电压钳法证明．Ｒｂ３３００ｍｇ·Ｌ－１使Ｌ、Ｂ、Ｔ型钙通道的开放时间缩短、开放概率减少，其作用与异搏定３７．５ｍｇ·Ｌ－１相似，与ＢａｙＫ８６１１５μｍｏｌ·Ｌ－１作用相反。确切地证明Ｒｂ３对钙通道有阻滞作用。     

DNA flow-cytometric analysis in colorectal cancer: A comparison of metastasizing and non-metastasizing tumours

The most common cause of death in patients with colorectal cancer is metastatic liver disease. In order to identify patients at a high risk of developing hepatic secondaries from colorectal cancers, DNA content was measured in metastasizing colorectal primaries (Group I, n= 32) as well as in their subsequently resected liver secondaries and in sections of non-metastasizing colorectal cancers (Group II, n= 25). A modified interpretation system involving both a DNA index and percentage of cycling cells (those in S and G2 + M phases) was developed. DNA content was measured in paraffin-embedded sections by flow cytometry using internal controls (human peripheral blood mononuclear cells) and non-malignant tissue controls (19 patients with diverticular disease). In Group I there were significantly more tumours with both abnormal ploidy (aneuploid or abnormal tetraploid peak) and > 15% cycling cells compared with Group II (Chi-squared; P= 0.034). The combination of abnormal ploidy and > 15% cycling cells was superior to Dukes’ classification for identifying metastasizing tumours (Logistic Regression; P= 0.047). However, it was not possible to discriminate between the two groups using either DNA ploidy or the percentage of cycling cells alone. The metastasizing colorectal cancers exhibited similar DNA ploidy characteristics and had a similar percentage of cycling cells compared with their liver metastases. These results suggest that tumour DNA ploidy plus the percentage of cycling cells may predict the development of liver metastases and thus survival in patients with colorectal cancer.     

Cloning of Human α-defensin-1 (HNP-1) Gene and Construction of Its Eukaryotic Expression Vector

1 Introduction Defensins are small cationic antimicrobial peptides that function in the host's innate immune system. The human defensin family includes three small peptides from the azurophil granules of polymorphonuclear cells named human neutrophil peptide (HNP)-1, HNP-2, HNP-3,which consist 5%-7% of the protein of human neutrophil. HNP-4 is approximately one hundred times less abundant. They demonstrate antibacterial, antifungal and antiviral properties in vitro. HNPs are important component of nonoxidative mechanism in macrophages, and can direct inactivate the enveloped viruses. Because only special cells express defensins. And it is hard to extract them naturally and the production is few. So researcher expect to obtain defensins highly through heterogenous expression by gene engineering technology. In order to express HNP-1, we cloned the cDNA of HNP-1 from human polymorphonuclear cells in peripheral blood, and constructed its eukaryotic expression vector, which provided a base for the further study on its mechanism of antimicrobial effect.