薤白对大鼠血清抗坏血酸自由基和血清发光的影响

本文采用电子自旋共振和化学发光技术，研究了薤白鲜汁对大鼠血清抗坏血酸自由基和血清发光的影响。结果表明，薤白鲜汁能使血清抗坏血酸自由基自旋浓度降低，呈量效关系，和抗氧化剂谷光甘肽情况类同。薤白汁使血清发光最大值和积分值均显著降低，谷光甘肽使血清发光最大值显著降低积分值呈升高趋势。提示薤白汁和谷光甘肽对血清发光的作用机理可能不同。进一步实验的结果显示薤白鲜汁和羟自由基清除剂甘露醇对血清发光特征的作用情     

八株弯曲菌外膜蛋白的SDS—PAGE分析

The outer membrane proteins (OMPs) from 8 strains of Campylobacter, including 5 reference strains and 3 local strains, were isolated and examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that the population distributions of molecular weight of OMPs from different species and sources were alike based on the Rank Sum Test analysis. The number of bands of major OMP was around 2-3, the molecular weight of which varied from 33-68 kd. Moreover, three C. jejuni local strains isolated from patients in China showed common characteristics of OMPs to the reference strains. It is suggested that the OMPs of Campylobacter are of a conservative and stable structure.     

虚拟现实技术在心理焦虑治疗中的应用

目前很多学生存在心理焦虑现象，这不仅影响他们的学习成绩，而且影响着他们的身心健康。传统的方法在治疗心理焦虑时效果会受到患者个人因素的影响，利用虚拟现实技术设计一套治疗方案，可以降低在治疗过程中患者个人因素的参与，增加患者对客观因素的感受，从而提高治疗效果。     

Tenecteplase vs. alteplase for the treatment of patients with acute ischemic stroke: a systematic review and meta-analysis

Journal of Neurology - At present, studies regarding the efficacy and safety of tenecteplase for the treatment of patients with acute ischemic stroke (AIS) are still limited and inconsistent. The...     

Variable frequency stimulation of sacral neuromodulation in black-zone overactive bladder patients: a case report

Overactive bladder (OAB) is a common urological disease, reducing patient quality of life (QoL). Sacral neuromodulation (SNM) is a treatment option used when conservative treatment is inadequate. However, constant frequency stimulation-SNM (CFS-SNM) may not be sufficiently effective in achieving targeted symptom reduction in some patients. For such black-zone patients, a different treatment strategy is needed. Variable frequency stimulation (VFS) has been used for deep-brain stimulation treatment in patients with Parkinson’s disease with positive outcomes. Accordingly, in this study, we hypothesized the promising outcomes of VFS-SNM in black-zone OAB patients. Here, we evaluated the efficacy and safety of VFS-SNM viz-a-viz CFS-SNM in a black-zone patient with refractory OAB whose frequent micturition symptoms were not relieved after undergoing traditional conservative treatment. A 50-year-old male patient was treated with CFS-SNM at our hospital in October 2016, but his symptoms recurred after administering multiple medications and program-controlled parameter adjustments. We then treated the patient with VFS-SNM in March 2020. A 2-week follow-up through telephonic interviews was conducted; the improvements in voiding symptoms were evaluated by calculating the OAB symptom score (OABSS) and OAB-related QoL (OAB-QoL) score. We observed that OABSS was significantly lower after VFS-SNM than after CFS-SNM. Further, we observed that VFS-SNM significantly improved daytime and nocturnal micturition frequency, as evident from the reduced OABSSs from after CFS-SNM to after VFS-SNM. The main reason for patient dissatisfaction after CFS-SNM was the increased average daily micturition frequency and urgency. VFS-SNM controlled the micturition frequency to within the patient’s acceptable range, significantly improving the patient’s QoL (40% improvement in OAB-QoL score). To the best of our knowledge, this is the first case report on the use of VFS-SNM with positive outcomes in a black-zone OAB patient, suggesting that VFS-SNM is not inferior to CFS-SNM in the treatment of black-zone patients.     

电针内关对心肌缺血再灌注损伤大鼠心肌细胞内钙离子浓度的调节

目的:与电针神门和合谷进行比较,以观察缺血再灌注损伤大鼠电针内关后心肌细胞内钙离子浓度的变化。方法:实验于2003-11/2004-02在湖南中医药大学针灸推拿系完成。选用雄性Wistar大鼠50只,体质量200～250g,购自河南医科大学实验动物中心,随机分为假手术组、模型组、内关组、神门组及合谷组,每组10只。采用经典的冠脉结扎法建立大鼠心肌缺血再灌注模型,双波长荧光分光光度计检测心肌细胞内钙离子浓度。结果:大鼠50只全部进入结果分析。大鼠缺血再灌注后心肌细胞内钙离子浓度增加,以模型组最高,明显高于假手术组(P<0.01)。经电针治疗各组细胞内钙离子浓度降低,神门组和内关组均明显低于模型组(P<0.05,0.01);而合谷组与模型组比较,差异不明显(P>0.05)。内关组细胞内钙离子浓度明显低于神门组和合谷组(P<0.01)。结论:①抑制心肌细胞内钙超载可能是电针内关对缺血再灌注损伤心肌保护的作用机制之一。②内关与神门疗效的差异性提示手厥阴心包经和心之间有相对特异性联系。     

The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation

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Folic acid functionalized aggregation-induced emission nanoparticles for tumor cell targeted imaging and photodynamic therapy

Recently, molecules with aggregation-induced luminescence (AIE) characteristics have received more and more attention due to the fluorescence of traditional dyes being easily quenched in the aggregated state. AIE molecules have significant advantages, such as excellent light stability, bright fluorescence, high contrast, and large Stokes shift. These characteristics have aroused wide interest of researchers and opened up new applications in many fields, especially in the field of biological applications. However, AIE molecules or their aggregates have certain limitations in multifunctional biological research due to their low specific targeting ability, poor biocompatibility, and poor stability in physiological body fluids. In order to overcome these problems, a novel nanoparticle, FFM1, was fabricated and characterized. FFM1 displayed good water solubility, biocompatibility, and AIE emission properties. It could target HeLa cells specifically by recognizing their folate receptor. Reactive oxygen triggered by light irradiation induced tumor cell apoptosis. Summarily, FFM1 displayed excellent capacity in target imaging and photodynamic killing of HeLa cells. It has shown potential application value in targeted diagnosis and photodynamic therapy of tumors, and has important guiding significance for the treatment of malignant tumors. It paves a way for the development of a novel strategy for tumor theranostics.

Herein, a novel nanoparticle, FFM1, displays good water solubility, biocompatibility and AIE emission properties. It has shown potential application value in targeted diagnosis and photodynamic therapy of tumors by recognizing folate receptor.     

Sacral anterior root stimulated defecation in spinal cord injuries： An experimental study in canine model

AIM: To investigate whether there was a dominant sacral root for the motive function of rectum and anal sphincter, and to provide an experimental basis for sacral root electrically stimulated defecation in spinal cord injuries. METHODS: Eleven spinal cord injured mongrel dogs were included in the study. After L4-L7 laminectomy, the bilateral L7-S3 roots were electrostimulated separately and rectal and sphincter pressure were recorded synchronously. Four animals were implanted electrodes on bilateral S2 roots. RESULTS: For rectal motorial innervation, S2 was the most dominant (mean 15.2 kPa, 37.7% of total pressure), S1(11.3 kPa, 27.6%) and S3 (10.9 kPa, 26.7%) contributed to a smaller part. For external anal sphincter, S3 (mean 17.2 kPa, 33.7%) was the most dominant, S2 (16.2 kPa, 31.6%) and S1(14.3 kPa, 27.9%) contributed to a lesser but still a significant part. Above 85% L7 roots provided some functional contribution to rectum and anal sphincter. For both rectum and sphincter, the right sacral roots provided more contribution than the left roots. Postoperatively, the 4 dogs had electrically stimulated defecation and micturition under the control of the neuroprosthetic device. CONCLUSION: S2 root is the most dominant contributor to rectal pressure in dogs. Stimulation of bilateral S2 with implanted electrodes contributes to good micturition and defecation in dogs.